Immunosensor Based on LongPeriod Fiber Gratings for Detection of Viruses Causing Gastroenteritis Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus viruslike particles (VLPs) is reported. The sensor is based on highly sensitive longperiod fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ngmL norovirus VLPs in a 40min assay in a labelfree manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the userxe2x80x99s safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFGbased biosensor could be applied in both fast norovirus detection and in vaccine testing.

Multiplexed Remote SPR Detection of Biological Interactions through Optical Fiber Bundles The development of sensitive methods for in situ detection of biomarkers is a real challenge to bring medical diagnosis a step forward. The proofofconcept of a remote multiplexed biomolecular interaction detection through a plasmonic optical fiber bundle is demonstrated here. The strategy relies on a fiber optic biosensor designed from a 300 xc2xb5m diameter bundle composed of 6000 individual optical fibers. When appropriately etched and metallized, each optical fiber exhibits specific plasmonic properties. The surface plasmon resonance phenomenon occurring at the surface of each fiber enables to measure biomolecular interactions, through the changes of the retroreflected light intensity due to lightplasmon coupling variations. The functionalization of the microstructured bundle by multiple protein probes was performed using new polymeric 3Dprinted microcantilevers. Such soft cantilevers allow for immobilizing the probes in micro spots, without damaging the optical microstructures nor the gold layer. We show here the potential of this device to perform the multiplexed detection of two different antibodies with limits of detection down to a few tenths of nanomoles per liter. This tool, adapted for multiparametric, realtime, and label free monitoring is minimally invasive and could then provide a useful platform for in vivo targeted molecular analysis.