Improving Allergenic Protein Prediction Using Physicochemical Features on NonRedundant Sequences Despite extensive studies in allergen prediction, current approaches still have room for performance improvement and suffer from the problem of lack of interpretable biological features. Thus, developments of allergen prediction method from sequences have become highly important to facilitate in silico vaccine design. In this study, we propose a systematic approach to predict allergenic proteins by incorporating sequence and physicochemical properties in machine learning algorithms. In addition, predictive performance of previous studies could be overestimated due to high redundancy in the data sets. Therefore, we reduce sequence redundancy in the data set and experiment results show that we achieve better predictive performance when compared with other approaches. This study can help discover new prophylactic and therapeutic vaccines for diseases. Moreover, we analyze immunological features that can provide valuable insights into immunotherapies of allergy and autoimmune diseases in translational bioinformatics.

Computational Prediction of Human BodyFluid Protein Research on body fluid proteomes has led to the discoveries of contextdependent proteomics profiles and numerous novel disease biomarkers. Common challenges remain with current proteomics technologies about how to effectively handle the large variety of protein modifications in those fluids. To this end, computational efforts have shown early successes in identifying biomarker proteins in specific human diseases. In this article, we first reviewed published computational methods on this topic and then presented a new database system and a novel deep learningbased method for predicting bodyfluid proteins in 16 types of human fluids. The results show that our new system outperforms existing methods and provides a highly promising tool to facilitate clinical proteomic discovery.