Patent application EP20174958A (EP), filed 2020-05-15
Publication number: EP3748006A1
Title: METHOD FOR PRODUCING NATURAL RUBBER, TRANSGENIC PLANT, METHOD FOR PRODUCING PNEUMATIC TIRE, AND METHOD FOR PRODUCING RUBBER PRODUCT
Abstract: [0001]    To provide methods for producing a natural rubber which enable the production of a natural rubber with an increased molecular weight, and transgenic plants which are able to produce a natural rubber with an increased molecular weight. Included is a method for producing a natural rubber, the method including the steps of: introducing a gene coding for a cis-prenyltransferase (CPT) family protein derived from a rubber-producing plant A into a rubber-producing plant B to prepare a transgenic plant C; and producing a natural rubber using the transgenic plant C, the rubber-producing plant A producing a natural rubber having a higher molecular weight than a natural rubber produced by the rubber-producing plant B not transfected with the gene.
IPC codes: A01H   5/    00            A I
B60C   1/    00            A I
C12N  15/    82            A I
C12N   9/    10            A I
Claims: number: 1
text: A method for producing a natural rubber, the method comprising the steps of:
number: 2
text: The method for producing a natural rubber according to claim 1,
wherein the gene coding for a cis-prenyltransferase (CPT) family protein is a gene coding for a cis-prenyltransferase (CPT) family protein derived from a plant of the genus Hevea, Ficus, Taraxacum, or Parthenium.
number: 3
text: The method for producing a natural rubber according to claim 1,
wherein the gene coding for a cis-prenyltransferase (CPT) family protein is a gene coding for a cis-prenyltransferase (CPT) family protein derived from Hevea brasiliensis, Taraxacum kok-saghyz, or Parthenium argentatum.
number: 4
text: A method for producing a pneumatic tire, the method comprising the steps of:
number: 5
text: A method for producing a rubber product, the method comprising the steps of:
number: 6
text: A transgenic plant, produced by introducing a gene coding for a cis-prenyltransferase (CPT) family protein derived from a rubber-producing plant A into a rubber-producing plant B,
the rubber-producing plant A producing a natural rubber having a higher molecular weight than a natural rubber produced by the rubber-producing plant B not transfected with the gene.
Office action: search_report, dated 2020-12-09
Rejection reasons: novelty_epc_54
Prior art cited: ref_id: JPS59140885A
ref_type: patent
severity: background
ref_id: JPS6070080A
ref_type: patent
severity: background
ref_id: WO9400977A1
ref_type: patent
severity: background
ref_id: JPS60251887A
ref_type: patent
severity: background
ref_id: JP2606856B2
ref_type: patent
severity: background
ref_id: JP2517813B2
ref_type: patent
severity: background
ref_id: JPH11127025A
ref_type: patent
severity: background
ref_id: - J. RUBB. RES., vol. 2, no. 3, pages 150 - 159
ref_type: npl
severity: background
ref_id: - PHYTOCHEMISTRY, vol. 67, 2006, pages 2590 - 2596
ref_type: npl
severity: background
ref_id: - PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
ref_type: npl
severity: background
ref_id: - METHODS ENZYMOL., vol. 183, 1990, pages 63
ref_type: npl
severity: background
ref_id: - "Molecular Cloning", 1994, COLD SPRING HARBOR PRESS, article "Methods for General and Molecular Bacteriology"
ref_type: npl
severity: background
ref_id: - "Immunology methods manual", ACADEMIC PRESS, article "Molecular"
ref_type: npl
severity: background
ref_id: - FUJIMURA ET AL., PLANT TISSUE CULTURE LETT., vol. 2, 1995, pages 74
ref_type: npl
severity: background
ref_id: - SHILLITO ET AL., BIO/TECHNOLOGY, vol. 7, 1989, pages 581
ref_type: npl
severity: background
ref_id: - VISSER ET AL., THEOR. APPL. GENET., vol. 78, 1989, pages 589
ref_type: npl
severity: background
ref_id: - AKAMA ET AL., PLANT CELL REP., vol. 12, 1992, pages 7
ref_type: npl
severity: background
ref_id: WO2004044173A2
ref_type: patent
severity: background
ref_id: EP3309257A1
ref_type: patent
severity: background
ref_id: WO2018116726A1
ref_type: patent
severity: background
ref_id: - GRABINSKA KARIONA A ET AL: "cis-Prenyltransferase: New Insights into Protein Glycosylation, Rubber Synthesis, and Human Diseases", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 291, no. 35, 26 August 2016 (2016-08-26), pages 18582 - 18590
ref_type: npl
severity: background
ref_id: - KHAREL YUGESH ET AL: "Identification of significant residues for homoallylic substrate binding of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 30, 27 July 2001 (2001-07-27), pages 28459 - 28464, ISSN: 0021-9258
ref_type: npl
severity: background
ref_id: - AKHTAR TARIQ A ET AL: "The tomato cisprenyltransferase gene family", PLANT JOURNAL, vol. 73, no. 4, February 2013 (2013-02-01), pages 640 - 652
ref_type: npl
severity: background
ref_id: WO2004044173
ref_type: patent
severity: novelty_destroying
ref_id: EP3309257
ref_type: patent
severity: novelty_destroying
ref_id: WO2018116726
ref_type: patent
severity: novelty_destroying
Final disposition: pending (2020-12-09)