Patent application EP19169321A (EP), filed 2019-04-15
Publication number: EP3725304A1
Title: PRODUCTION OF SALIPRO PARTICLES
Abstract: The invention relates to a process for preparing saposin lipoprotein particles, comprising a saposin-like protein, lipids and optionally a hydrophobic agent wherein the saposin-like protein or the hydrophobic agent is selectively bound to a support to allow the self-assembly of the saposin lipoprotein particles. The process of the invention comprises the step of a.) providing the hydrophobic agent and lipids, b.1)/b.2) contacting the hydrophobic agent or the saposin-like protein with a support that is capable of selectively binding either of the two molecules to the support, c.1)/c.2) contacting the support-bound particle components with the remaining particle components, either the saposin-like protein or the hydrophobic agent, to allow for the self-assembly of the saposin lipoprotein particle on the support and d.) optionally eluting the support-bound saposin lipoprotein particles.
IPC codes: A61K  38/    00            A I
A61K  47/    24            A I
A61K  47/    42            A I
A61K   9/   127            A I
Claims: number: 1
text: Process for producing a saposin lipoprotein particle, wherein the produced saposin lipoprotein particle comprises
number: 2
text: Process according to claim 1, alternative (I), wherein the support comprises a capture moiety, and the saposin-like protein comprises a binding moiety, wherein the capture moiety is capable of selectively binding the binding moiety in the saposin-like protein.
number: 3
text: Process according to claim 1, alternative (II), wherein the support comprises a capture moiety, and the hydrophobic agent comprises a binding moiety, wherein the capture moiety is capable of selectively binding the binding moiety in the hydrophobic agent.
number: 4
text: Process according to any one of the preceding claims, wherein the support is in the form of
number: 5
text: Process according to any one of the preceding claims, wherein the lipids are selected from the group consisting of viral, archaeal, eukaryotic and prokaryotic lipids, and mixtures thereof.
number: 6
text: Process according to any one of the preceding claims, wherein in step a) the hydrophobic agent and the lipids are provided in form of a viral, archaeal, eukaryotic or prokaryotic membrane, which comprises the hydrophobic agent and the lipids that are to be incorporated into the saposin lipoprotein particles.
number: 7
text: Process according to claim 6 in so far as it relates to claim 1, alternative (I), wherein in step c.1) the support-bound saposin-like protein is contacted with the viral, archaeal, eukaryotic or prokaryotic membrane provided in step a) to allow formation of a library of saposin like particles wherein the library comprises a heterogenic mixture of saposin lipoprotein particles with different membrane lipid and optionally membrane protein compositions.
number: 8
text: Process according to any one of the preceding claims, wherein the saposin-like protein is saposin A, saposin B, saposin C, saposin D or a derivative or truncated form thereof, which is capable of forming saposin lipoprotein particles in the process of claim 1.
number: 9
text: Process according to claim 8, wherein the derivative or truncated form is selected from
number: 10
text: Process according to any one of the preceding claims, wherein the hydrophobic agent is selected from the group consisting of a hydrophobic organic compound and a hydrophobic biomolecule.
number: 11
text: Process according to claim 10, wherein the hydrophobic organic compound and/or the hydrophobic biomolecule is selected from the group consisting of a biologically active agent, a drug, an active ingredient of a drug, an active ingredient of a cosmetic product, an active ingredient of a plant protective product, a dietary and/or nutritional supplement, a diagnostic probe, a contrast agent, a label and an indicator.
number: 12
text: Process according to any one of claims 10 or 11, wherein the hydrophobic biomolecule is a protein comprising a hydrophobic moiety, in particular a protein selected from the group consisting of a membrane protein, an integral transmembrane protein, an integral monotopic membrane protein, a peripheral membrane protein, an amphitropic protein in a lipid-bound state, a lipid-anchored protein and a chimeric protein with a fused hydrophobic and/or transmembrane domain.
number: 13
text: Process according to any one of the preceding claims, wherein the hydrophobic agent, the lipids and/or the saposin-like protein is in a detergent-solubilized state and wherein optionally the detergent is selected from the group consisting of alkylbenzenesulfonates or bile acids, cationic detergents and nonionic or zwitterionic detergents such as lauryl-dimethyl amine-oxides (LDAO), Fos-Cholines, CHAPS/CHAPSO, saponins such as Digitonin and structurally related synthetic detergents such as glycol-diosgenin, alkyl glycosides such as short, medium or longer chain alkyl maltosides, in particular n-Dodecyl β-D-maltoside, glucosides, maltose-neopentyl glycol (MNG) amphiphiles, amphiphilic polymers (amphipols), styrene maleic acid co-polymer (SMA), macrocycle or cyclic oligomers based on a hydroxyalkylation product of a phenol and an aldehyde (Calixarene), and mixtures thereof.
number: 14
text: Process according to any one of the preceding claims, wherein
Office action: search_report, dated 2020-10-21
Prior art cited: ref_id: EP1596828A2
ref_type: patent
severity: background
ref_id: EP1345959A2
ref_type: patent
severity: background
ref_id: WO2014095576A1
ref_type: patent
severity: background
ref_id: WO2015036549A1
ref_type: patent
severity: background
ref_id: WO2018033647A1
ref_type: patent
severity: background
ref_id: - WALLIN; VON HEIJNE, PROTEIN SCIENCE, vol. 7, no. 4, April 1998 (1998-04-01), pages 1029 - 38
ref_type: npl
severity: background
ref_id: - OVERINGTON ET AL., NATURE REVIEWS DRUG DISCOVERY, vol. 5, December 2006 (2006-12-01), pages 993 - 996
ref_type: npl
severity: background
ref_id: - ALLEN; CULLIS, SCIENCE, vol. 303, no. 5665, 19 March 2004 (2004-03-19), pages 1818 - 1822
ref_type: npl
severity: background
ref_id: - BAYBURT ET AL., ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 450.2, 2006, pages 215 - 222
ref_type: npl
severity: background
ref_id: - DENISOV ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126.11, 2004, pages 3477 - 3487
ref_type: npl
severity: background
ref_id: - BRUHN, BIOCHEM J., vol. 389, 2005, pages 249 - 257
ref_type: npl
severity: background
ref_id: - O'BRIEN ET AL., SCIENCE, vol. 241, 1988, pages 1098 - 1101
ref_type: npl
severity: background
ref_id: - FURST ET AL., BIOCHIM BIOPHYS ACTA, vol. 1126, 1992, pages 1 - 16
ref_type: npl
severity: background
ref_id: - ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 415, no. 1, 1 July 2003 (2003-07-01), pages 43 - 53
ref_type: npl
severity: background
ref_id: - QI ET AL., CLIN CANCER RES, vol. 15, no. 18, 2009, pages 5840 - 5851
ref_type: npl
severity: background
ref_id: - POPOVIC ET AL., PNAS, vol. 109, no. 8, 2012, pages 2908 - 2912
ref_type: npl
severity: background
ref_id: - MUNFORD ET AL., JOURNAL OF LIPID RESEARCH, vol. 36, no. 8, 1995, pages 1653 - 1663
ref_type: npl
severity: background
ref_id: - BRUHN, BIOCHEM J, vol. 389, no. 15, 2005, pages 249 - 257
ref_type: npl
severity: background
ref_id: - AHN ET AL., PROTEIN SCI., vol. 15, 2006, pages 1849 - 1857
ref_type: npl
severity: background
ref_id: - DE ALBA ET AL., BIOCHEMISTRY, vol. 42, 2003, pages 14729 - 14740
ref_type: npl
severity: background
ref_id: - LIEPINSH ET AL., NAT. STRUCT. BIOL., vol. 4, 1997, pages 793 - 795
ref_type: npl
severity: background
ref_id: - ANDERSON ET AL., J. MOL. BIOL., vol. 325, 2003, pages 355 - 365
ref_type: npl
severity: background
ref_id: - HAWKINS ET AL., J.MOL.BIOL., vol. 346, 2005, pages 1381 - 1392
ref_type: npl
severity: background
ref_id: - POPOVIC, PNAS, vol. 109, no. 8, 2012, pages 2908 - 2912
ref_type: npl
severity: background
ref_id: - HENIKOFF S.; HENIKOFF JG., P. N. A. S. USA, vol. 89, 1992, pages 10915 - 10919
ref_type: npl
severity: background
ref_id: - NEEDLEMAN; WUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
ref_type: npl
severity: background
ref_id: - CAHN, R.S.; INGOLD, C.K.; PRELOG, V.: "Angewandte Chemie", vol. 5, 1966, article "Specification of Molecular Chirality", pages: 385 - 415
ref_type: npl
severity: background
ref_id: - ALBERTS ET AL.: "The Cell", 2002, MACMILLIAN MAGAZINES LTD, pages: 61,62
ref_type: npl
severity: background
ref_id: - "handbook", ANATRACE, article "Detergents and their uses in membrane protein science"
ref_type: npl
severity: background
ref_id: - ALBERTS ET AL.: "The Cell", 2002, MACMILLIAN MAGAZINES LTD, pages: 583 - 614
ref_type: npl
severity: background
ref_id: - CAMPBELL ET AL.: "Biologie", 2003, SPEKTRUM VERLAG, pages: 163 - 177
ref_type: npl
severity: background
ref_id: - DE ROSA ET AL.: "Structure, Biosynthesis, and Physicochemical Properties of Archaebacterial Lipids", MICROBIOLOGICAL REVIEWS, vol. 50, no. 1, 1986, pages 70 - 80
ref_type: npl
severity: background
ref_id: - ALBERS ET AL.: "The archaeal cell envelope", NATURE REVIEWS MICROBIOLOGY, vol. 9, 2011, pages 414 - 426
ref_type: npl
severity: background
ref_id: - LORIZATE ET AL.: "Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines", CELLULAR MICROBIOLOGY, vol. 15, no. 2, 2013, pages 292 - 304
ref_type: npl
severity: background
ref_id: - BRIIGGER ET AL.: "The HIV lipidome: A raft with an unusual composition", PNAS, vol. 103, no. 8, 2006, pages 2641 - 2646
ref_type: npl
severity: background
ref_id: EP2745834A1
ref_type: patent
severity: background
ref_id: EP3284460A1
ref_type: patent
severity: background
ref_id: - FRAUENFELD J ET AL: "A saposin-lipoprotein nanoparticle system for membrane proteins", NATURE METHODS, NATURE PUB. GROUP, NEW YORK, vol. 13, no. 4, 1 April 2016 (2016-04-01), pages 345, XP002766266, ISSN: 1548-7091, [retrieved on 20160307], DOI: 10.1038/NMETH.3801
ref_type: npl
severity: background
ref_id: EP2745834
ref_type: patent
severity: background
ref_id: EP3284460
ref_type: patent
severity: background
ref_id: - FRAUENFELD J ET AL, "A saposin-lipoprotein nanoparticle system for membrane proteins", NATURE METHODS, NATURE PUB. GROUP, NEW YORK, vol. 13, no. 4, doi:10.1038/NMETH.3801, ISSN 1548-7091, (20160401), page 345, (20160307), XP002766266 [A] 1-14 * the whole document *
ref_type: npl
severity: background
Final disposition: pending (2020-10-21)