Patent application EP19183486A (EP), filed 2019-06-28
Publication number: EP3757219A1
Title: ENHANCED PLANT REGENERATION AND TRANSFORMATION BY USING GRF1 BOOSTER GENE
Abstract: [0001]    The present invention relates to the field of plant breeding and biotechnology and in particular to the generation of plants from cells and other tissues. More particularly, the invention provides methods and means for improving plant regeneration, especially from transformed or genetically modified plant cells using GRF1 booster gene.
IPC codes: A01H   1/    08            A I
A01H   4/    00            A I
A01H   5/    00            A I
C12N  15/    82            A I
Claims: number: 1
text: A method for transforming a plant cell, comprising the steps
number: 2
text: A method for modifying the genome of a plant cell, comprising the steps (a1
number: 3
text: A method of producing a transgenic plant, comprising the steps
number: 4
text: A method of producing a genetically modified plant, comprising the steps
number: 5
text: A method of producing a haploid plant embryo, comprising the steps (a1
number: 6
text: The method of any one of the claims 1 to 5, wherein the GRF1 polypeptide comprises a PFAM domain PF08880 and a PFAM domain PF08879, preferably wherein the PFAM domain PF08880 finds a match of at least 90% coverage at or near the N-terminus of the GRF1 polypeptide and the PFAM domain PF08879 finds a match of at least 90% C-terminally located to the PFAM domain PF08880 in the GRF1 polypeptide.
number: 7
text: The method of claims 6, wherein both matching amino acid stretches are located in the N-terminal half of the GRF1 polypeptide.
number: 8
text: The method of any one of the claims 1 to 7, wherein the GRF1 polypeptide comprises the motif [D]-[P]-[E]-[P]-[G]-[R]-[C]-[R]-[R]-[T]-[D]-[G]-[K]-[K]-[W]-[R]-[C]-[AS]-[RK]-[ED]-[A]-[AH]-[PQS]-[D]-[S]-[K]-[Y]-[C]-[E]-[KR]-[H]-[M]-[H]-[R]-[G]-[R]-[N]-[R] (SEQ ID NO: 25) with a maximum number of two mismatches, wherein preferably the motif consists of any of the amino acid sequences SEQ ID NO: 26 to SEQ ID NO: 35, and/or wherein preferably the motif contains a sub-region of amino acid stretch matching PFAM domain PF08879.
number: 9
text: The method of any one of the claims 1 to 8, wherein the GRF1 polypeptide comprises
number: 10
text: The method of any one of the claims 1 to 9, wherein the polynucleotide encoding the GRF1 polypeptide comprises
number: 11
text: The method of any one of the claims 1 to 10, wherein introducing into a plant cell the expression cassette comprising a polynucleotide encoding a GRF1 polypeptide results in a stable integration thereof into the genome of the plant cell, or wherein introducing into a plant cell the expression cassette comprising a polynucleotide encoding a GRF1 polypeptide, mRNA encoding GRF1 polypeptide, or GRF1 polypeptide(s) or inducing in a plant cell the enhanced expression level of an endogenous gene encoding a GRF1 polypeptide results in a transient occurrence of GRF1 polypeptide(s) in the plant cell or in a progeny cell thereof.
number: 12
text: The method of any one of the claims 1 to 11, wherein the polynucleotide encoding the GRF1 polypeptide is in operative linkage to at least one regulatory sequence suitable for expression of the GRF1 polypeptide in a plant cell.
number: 13
text: The method of any one of claims 1 to 4, wherein the plant cell of step (a1) or (a2) is a cell of a somatic tissue, callus tissue, a meristematic tissue or an embryonic tissue, a protoplast, gametophyte, pollen, ovule or microspore.
number: 14
text: A plant or part thereof obtained or obtainable by the method of claim 3 or 4, or a progeny plant thereof.
number: 15
text: A plant cell or a seed of the plant of claim 14, wherein the plant cell or the seed comprises the at least one nucleotide sequence of interest as transgene or comprises the modification in the genome.
Office action: search_report, dated 2020-12-30
Rejection reasons: novelty_epc_54
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severity: novelty_destroying
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severity: background
Final disposition: pending (2020-12-30)