Patent application EP19305866A (EP), filed 2019-06-27
Publication number: EP3757572A1
Title: DETECTION OF PATHOGENIC BACTERIA BY CHEMICALLY FUNCTIONALIZED CELLULOSE
Abstract: The present invention concerns the rapid and selective detection of pathogenic bacteria expressing a lectin-type adhesin in a biological environment using a cellulose support functionalized with a ligand of a lectin-type adhesin, and its use for the diagnostic of infection with pathogenic bacteria expressing a lectin-type adhesin, for example in the context of Crohn's disease or in urinary tract infections.
IPC codes: A61K  47/    38            A I
A61P  31/    04            A I
A61P  35/    00            A I
G01N  33/   569            A I
Claims: number: 1
text: Method for detecting in vitro pathogenic bacteria expressing a lectin-type adhesin in a subject biological sample, comprising:
number: 2
text: Method according to claim 1, wherein the lectin-type adhesin is FimH adhesin.
number: 3
text: Method according to either one of claim 1 or 2, wherein the ligand of the lectin-type adhesin is a mannose derivative, preferably a heptylmannoside (HMan) or a derivative thereof.
number: 4
text: Method according to anyone of claims 1 to 3, wherein the subject biological sample to be tested is chosen from the group consisting of bacterial culture, feces, urine, intestinal tissues.
number: 5
text: Method according to any one of claims 1 to 4, wherein the pathogenic bacteria expressing the lectin-type adhesin are Adherent Invasive E. coli (AIECs).
number: 6
text: Method for identifying a host having a pathology caused by pathogenic bacteria expressing a lectin-type adhesin and mediated by interactions between lectin-type adhesin and host cell surface glycans, comprising using the method for detecting of any one of claims 1 to 5.
number: 7
text: Method according to claim 6, wherein the pathology is chosen in the group consisting of:
number: 8
text: Method according to either one of claims 6 or 7, wherein the lectin-type adhesin is FimH adhesin.
number: 9
text: A kit for detecting pathogenic bacteria expressing a lectin-type adhesin in a subject biological sample, comprising at least a cellulose support functionalized with a ligand of a lectin-type adhesin, and at least a mean of colorimetric or fluorescence or luminescence detection for detecting trapped pathogenic bacteria expressing the lectin-type adhesin.
number: 10
text: A kit according to claim 9, wherein the pathogenic bacteria expressing a lectin-type adhesin are Adherent Invasive E. coli (AIECs).
number: 11
text: A kit according to either one of claims 9 or 10, wherein the lectin-type adhesin is FimH adhesin.
number: 12
text: Cellulose support functionalized with a ligand of a lectin-type adhesin for use in the treatment or prevention of a pathology caused by pathogenic bacteria expressing a lectin-type adhesin and mediated by interactions between lectin-type adhesin and host cell surface glycans.
number: 13
text: Functionalized-cellulose support for use according to claim 12, wherein the pathology is chosen in the group consisting of:
number: 14
text: Functionalized-cellulose support for use according to either one of claim 12 or 13, wherein the lectin-type adhesin is FimH adhesin.
Office action: search_report, dated 2020-12-30
Rejection reasons: novelty_epc_54
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ref_id: - MADELEINE CAUWEL ET AL: "Heptylmannose-functionalized cellulose for the binding and specific detection of pathogenic E. coli", CHEMICAL COMMUNICATIONS, vol. 55, no. 68, 20 August 2019 (2019-08-20), UK, pages 10158 - 10161, XP055653454, ISSN: 1359-7345, DOI: 10.1039/C9CC05545B
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ref_id: - HONG DONG ET AL, "Biofunctionalized Cellulose Nanofibrils Capable of Capture and Antiadhesion of Fimbriated Escherichia coli", ACS APPLIED BIO MATERIALS, (20190523), vol. 2, no. 7, doi:10.1021/acsabm.9b00295, ISSN 2576-6422, pages 2937 - 2945, XP055653530 [X] 1-14 * the whole document, in particular abstract, scheme 1; sections "Experimental Methods" and "Conclusions" *
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severity: novelty_destroying
ref_id: - MADELEINE CAUWEL ET AL, "Heptylmannose-functionalized cellulose for the binding and specific detection of pathogenic E. coli", CHEMICAL COMMUNICATIONS, UK, (20190820), vol. 55, no. 68, doi:10.1039/C9CC05545B, ISSN 1359-7345, pages 10158 - 10161, XP055653454 [T] * the whole document *
ref_type: npl
severity: background
Final disposition: pending (2020-12-30)