Patent application EP19155627A (EP), filed 2019-02-05
Publication number: EP3693465A1
Title: NOVEL FISH CORONAVIRUS
Abstract: The invention relates to a novel fish virus, indicated to be a Coronavirus, which causes mortality in fish, and to methods of detecting said virus in fish, and related uses.
IPC codes: A61K  39/   215            A I
C07K  14/   165            A I
C07K  16/    08            A I
C12N  15/   113            A I
C12N  15/    50            A I
C12N   7/    00            A I
C12Q   1/    68            A I
Claims: number: 1
text: A nucleic acid, wherein the sequence of said nucleic acid comprises at least one open reading frame (ORF) sequence selected from the group consisting of ORF-1, ORF-2, ORF-3, ORF-4 and ORF-5; wherein
number: 2
text: The nucleic acid of claim 1, wherein
number: 3
text: A nucleic acid, wherein the sequence of said nucleic acid is complementary to the sequence of the nucleic acid claimed in claim 1 or 2.
number: 4
text: A virus that infects and is capable of killing lumpsucker fish (Cyclopterus lumpus), wherein the virus genome comprises the sequence of the nucleic acid of any one of claims 1 to 3, wherein said nucleic acid sequence contains the base uracil (U) instead of the base thymine (T).
number: 5
text: The virus of claim 4, wherein the infection of lumpsucker fish by the virus causes the following symptoms in the fish:
number: 6
text: The virus of claim 4 or claim 5, wherein
number: 7
text: An oligonucleotide primer which
number: 8
text: Use of at least one oligonucleotide primer in a method of detecting the virus of any one of claims 4 to 6, wherein the at least one primer comprises a sequence of at least 9 nucleotides and wherein said sequence is complementary to a nucleic acid sequence which is comprised within the genome of said virus.
number: 9
text: A method for detecting a virus that infects and is capable of killing fish, comprising the steps of:
number: 10
text: A method for detecting a virus that infects and is capable of killing fish, comprising the steps of:
number: 11
text: An antibody that binds a polypeptide, wherein the polypeptide is encoded by a nucleic acid sequence which is comprised within the genome of the virus of any one of claims 4 to 6, optionally wherein the polypeptide is selected from the group consisting of:
number: 12
text: A kit for detecting a virus in a biological sample from fish, wherein the kit comprises an oligonucleotide primer of claim 7 and/or an antibody of claim 11,
optionally wherein the kit is a real-time RT-PCR assay.
number: 13
text: The antibody of claim 11 for use in treating fish infected with a virus of any one of claims 4 to 6.
number: 14
text: Use of the virus of any one of claims 4 to 6 for producing a vaccine.
number: 15
text: A vaccine comprising:
number: 16
text: The vaccine of claim 15, wherein the sequence of the nucleic acid is the sequence of the nucleic acid claimed in any one of claims 1 to 3, wherein said nucleic acid sequence contains the base uracil (U) instead of the base thymine (T).
number: 17
text: An interfering RNA (iRNA) molecule for use in treating fish infected with a virus, wherein the iRNA molecule comprises at least 12 nucleotides of, or complimentary to, a nucleic acid sequence comprised within the genome of the virus of any one of claims 4 to 6.
Office action: search_report, dated 2020-08-12
Rejection reasons: novelty_epc_54
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Final disposition: pending (2020-08-12)