Patent application EP19167093A (EP), filed 2019-04-03
Publication number: EP3718397A1
Title: WHEAT CYTOPLASMIC MALE STERILITY RESTORER GENES, MOLECULAR MARKERS AND USES THEREOF
Abstract: The present invention provides functional restorer genes for cytoplasmic male sterility. The invention inter alia relates to functional restorer gene alleles localizing to a position corresponding to or referred to 14584864 to 14587134 of chromosome 1A of Chinese Spring wheat; or 19061029 to 19063412 of chromosome 1B of Chinese Spring wheat. The invention also relates to nucleic acids, polypeptides, marker alleles and SNPs.
IPC codes: A01H   1/    04            A I
A01H   5/    10            A I
A01H   6/    46            A I
C07K  14/   415            A I
C12N  15/    82            A I
C12Q   1/  6895            A I
Claims: number: 1
text: A nucleic acid comprising a nucleic acid sequence
number: 2
text: A nucleic acid comprising a functional restorer gene allele localizing to a position referred to as position
number: 3
text: A nucleic acid comprising a functional restorer gene allele localizing to a position
number: 4
text: The nucleic acid of any one of claims 1-3, wherein the functional restorer gene allele is for restoring G-type wheat cytoplasmic male sterility.
number: 5
text: A vector or expression cassette comprising the nucleic acid of any one of claims 1-4.
number: 6
text: A polypeptide encoded by the nucleic acid of any one of claims 1-4.
number: 7
text: A recombinant gene comprising the following operably linked elements
number: 8
text: A method for producing a plant cell, a plant or part thereof or a seed comprising a functional restorer gene allele for restoring cytoplasmic male sterility or for converting a non-restoring plant into a restoring plant comprising a functional restorer gene allele for restoring cytoplasmic male sterility, wherein the method comprises
number: 9
text: A method for identifying or selecting a plant cell, a plant or part thereof or a seed comprising a functional restorer gene allele for restoring cytoplasmic male sterility comprising the step of detecting or identifying in said plant cell, said plant or said part thereof or said seed the presence of the nucleic acid of any one of claims 1-4, the vector or expression cassette of claim 5, the polypeptide of claim 6 or the recombinant gene of claim 7.
number: 10
text: A method for identifying or selecting a wheat plant cell, a wheat plant or part thereof or a wheat seed comprising a functional restorer gene allele for wheat G-type cytoplasmic male sterility located on chromosome 1A and/or a functional restorer gene allele for wheat G-type cytoplasmic male sterility located on chromosome 1B, comprising the steps of:
number: 11
text: A plant cell, a plant or part thereof or a seed obtained by the method of claim 8, or identified by the method of claim 9 or 10, or comprising the nucleic acids according to any one of claims 1-4, the vector or expression cassette of claim 5 or the recombinant genes of claim 7.
number: 12
text: A method for determining the presence, absence or zygosity status of a functional restorer gene allele for wheat G-type cytoplasmic male sterility located on chromosome 1A and/or on chromosome 1B in a sample derived from a wheat plant, comprising the steps of
number: 13
text: The method of claim 10 or claim 12, wherein
number: 14
text: Use of an oligonucleotide comprising a nucleic acid sequence according to any one of SEQ ID NOs: 47-225, wherein the nucleotide position of the SNP is shown in column 2 of the respective tables 3-6, and the nucleotide at this position is shown in column 5 of the respective tables 3-6, or a SNP selected from the group of SNPs shown in tables 3-6, wherein the nucleotide position of the SNP is shown in column 2 of the respective table, and the nucleotide at this position is shown in column 5 of the respective table, in a method for identifying one or more functional restorer gene alleles in a wheat plant.
Office action: search_report, dated 2020-10-07
Rejection reasons: novelty_epc_54
Prior art cited: ref_id: WO2018015403A1
ref_type: patent
severity: background
ref_id: WO2018015404A1
ref_type: patent
severity: background
ref_id: - LURIN ET AL., THE PLANT CELL, vol. 16, no. 8, 2004, pages 2089 - 2103
ref_type: npl
severity: background
ref_id: - SCHMITZ-LINNEWEBER; SMALL, TRENDS IN PLANT SCIENCE, vol. 13, no. 12, 2008, pages 663 - 670
ref_type: npl
severity: background
ref_id: - DAHAN; MIREAU, RNA BIOLOGY, vol. 10, no. 9, 2013, pages 1469 - 1476
ref_type: npl
severity: background
ref_id: - MELONEK ET AL., SCIENTIFIC REPORTS, vol. 6, 2016, pages 35152
ref_type: npl
severity: background
ref_id: - CHENG, S.; GUTMANN, B.; ZHONG, X.; YE, Y.; FISCHER, M.; BAI, F.; CASTLEDEN, I.; SONG, Y.; SONG, B.; HUANG, J.: "Redefining the structural motifs that determine RNA binding and RNA editing by pentatricopeptide repeat proteins in land plants", THE PLANT JOURNAL, vol. 85, 2016, pages 532 - 547
ref_type: npl
severity: background
ref_id: - HMMER 3.1B2, February 2015 (2015-02-01), Retrieved from the Internet <URL:http://hmmer.org>
ref_type: npl
severity: background
ref_id: - KAUL: "Male sterility in higher plants", 1988, SPRINGER VERLAG
ref_type: npl
severity: background
ref_id: - MUKAI; TSUNEWAKI, THEOR. APPL. GENET., vol. 54, 1979
ref_type: npl
severity: background
ref_id: - TSUNEWAKI, JPN. SOC. PROM. SCI., 1980
ref_type: npl
severity: background
ref_id: - TSUNEWAKI ET AL., GENES GENET. SYST., vol. 71, 1996
ref_type: npl
severity: background
ref_id: - ALLEN ET AL., PLANT BIOTECHNOLOGY J., vol. 9, no. 1086-1099, 2011, pages 1097 - 1098
ref_type: npl
severity: background
ref_id: - MANNA ET AL., BIOCHEMIE, vol. 113, 2015, pages 93 - 99
ref_type: npl
severity: background
ref_id: - FUJI ET AL., PNAS, vol. 108, no. 4, 2011, pages 1723 - 1728
ref_type: npl
severity: background
ref_id: - MELONEK ET AL., NAT SCI REPORT, vol. 6, 2016, pages 35152
ref_type: npl
severity: background
ref_id: - BARKAN ET AL., PLOS GENET, vol. 8, no. 8, 2012, pages el002910
ref_type: npl
severity: background
ref_id: - AHMED ET AL.: "QTL analysis of fertility-restoration against cytoplasmic male sterility in wheat", GENES GENET SYST, vol. 76, 2001, pages 33 - 38, XP055358955, DOI: doi:10.1266/ggs.76.33
ref_type: npl
severity: background
ref_id: - GEYER ET AL., MOL BREEDING, vol. 36, 2016, pages 167
ref_type: npl
severity: background
ref_id: - MOL GENETICS AND GENOMICS, vol. 293, 2018, pages 451 - 462
ref_type: npl
severity: background
ref_id: - KOMORI T; OHTA S; MURAI N; TAKAKURA Y; KURAYA Y; SUZUKI S; HIEI Y; IMASEKI H; NITTA N: "Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza sativa L.", THE PLANT JOURNAL, vol. 37, 2004, pages 315 - 325, XP002310740
ref_type: npl
severity: background
ref_id: - J. A. DOUDNA; E. CHARPENTIER: "The new frontier of genome engineering with CRISPR-Cas9", SCIENCE, vol. 346, 2014, XP055162699, DOI: doi:10.1126/science.1258096
ref_type: npl
severity: background
ref_id: - J. D. SANDER; J. K. JOUNG: "CRISPR-Cas systems for editing, regulating and targeting genomes", NAT BIOTECHNOL, vol. 32, 2014, pages 347, XP055481941, DOI: doi:10.1038/nbt.2842
ref_type: npl
severity: background
ref_id: WO2018015403A1
ref_type: patent
severity: background
ref_id: WO2017158127A1
ref_type: patent
severity: background
ref_id: WO2019086510A1
ref_type: patent
severity: background
ref_id: - GEYER MANUEL ET AL: "Distribution of the fertility-restoring geneRf3in common and spelt wheat determined by an informative SNP marker", MOLECULAR BREEDING: NEW STRATEGIES IN PLANT IMPROVEMENT, KLUWER ACADEMIC PUBLISHERS, NL, vol. 36, no. 12, 19 December 2016 (2016-12-19), pages 1 - 11, XP036125222, ISSN: 1380-3743, [retrieved on 20161219], DOI: 10.1007/S11032-016-0592-6
ref_type: npl
severity: background
ref_id: - WÜRSCHUM TOBIAS ET AL: "Genetic architecture of male fertility restoration ofTriticum timopheeviicytoplasm and fine-mapping of the major restorer locusRf3on chromosome 1B", THEORETICAL AND APPLIED GENETICS ; INTERNATIONAL JOURNAL OF PLANT BREEDING RESEARCH, SPRINGER, BERLIN, DE, vol. 130, no. 6, 21 March 2017 (2017-03-21), pages 1253 - 1266, XP036240673, ISSN: 0040-5752, [retrieved on 20170321], DOI: 10.1007/S00122-017-2885-5
ref_type: npl
severity: background
ref_id: WO2017158127
ref_type: patent
severity: novelty_destroying
ref_id: WO2018015403
ref_type: patent
severity: novelty_destroying
ref_id: WO2019086510
ref_type: patent
severity: novelty_destroying
ref_id: - GEYER MANUEL ET AL, "Distribution of the fertility-restoring geneRf3in common and spelt wheat determined by an informative SNP marker", MOLECULAR BREEDING: NEW STRATEGIES IN PLANT IMPROVEMENT, KLUWER ACADEMIC PUBLISHERS, NL, vol. 36, no. 12, doi:10.1007/S11032-016-0592-6, ISSN 1380-3743, (20161219), pages 1 - 11, (20161219), XP036125222 [ID] 1-14 * abstract; pages 2, 4, 5, 10, 11; Figure 1 *
ref_type: npl
severity: background
ref_id: - WÜRSCHUM TOBIAS ET AL, "Genetic architecture of male fertility restoration ofTriticum timopheeviicytoplasm and fine-mapping of the major restorer locusRf3on chromosome 1B", THEORETICAL AND APPLIED GENETICS ; INTERNATIONAL JOURNAL OF PLANT BREEDING RESEARCH, SPRINGER, BERLIN, DE, vol. 130, no. 6, doi:10.1007/S00122-017-2885-5, ISSN 0040-5752, (20170321), pages 1253 - 1266, (20170321), XP036240673 [I] 1-14 * abstract; pages 1254, 1256, 1259, 1262-1264 *
ref_type: npl
severity: background
Final disposition: pending (2020-10-07)