Patent application EP17173678A (EP), filed 2017-05-31
Publication number: EP3251690A1
Title: MT-45 IMMUNODETECTION
Abstract: Components for enabling immunodection of MT-45 are described including immunogens, antibodies derived from the immunogens, immunoassay methods, detecting agents and kits.
IPC codes: A61K  39/    00            A I
A61K  39/   385            A I
G01N  33/   487            A I
G01N  33/    94            A I
Claims: number: 1
text: An immunogen of structure
number: 2
text: The immunogen of claim 1 in which R is attached to the meta or para position of the phenyl ring.
number: 3
text: The immunogen of claim 2 in which R is -(Q)m-Y-X-, in which Q or Y (if Q is not present) is attached to the meta or para position of the phenyl ring and m= 0 or 1; Y can comprise a substituted or unsubstituted, straight or branched chain, alkanediyl moiety, a substituted or unsubstituted, saturated or unsaturated cycloalkylene moiety or a substituted or unsubstituted arylene moiety; X, before attachment to the antigenicity conferring carrier material, is chosen from carboxy , dithiopyridyl, maleimidyl, amino, hydroxyl, thiol , thioester, or aldehyde; Q is -O-, -S-, -NH-, -C(O)-, -C(O)-O-., -S(O)-, -S(O)<2> -, -C(S)-O- or -C(O)-S-.
number: 4
text: The immunogen of claim 3 in which m=1 and Q is -O-, -NH- or -S-, and Y is a C<1-6> substituted or unsubstituted, straight chain, alkanediyl moiety.
number: 5
text: The immunogen of claim 4 in which Q is attached to the meta position of the phenyl ring.
number: 6
text: The immunogen of any of the preceding claims in which the antigenicity conferring carrier material is keyhole limpet haemocyanin, bovine thyroglobulin, bovine serum albumin, egg ovalbumin, bovine gamma globulin or cationised BSA.
number: 7
text: An antibody derived from an immunogen of any of claims 2 to 6 which binds to MT-45.
number: 8
text: The antibody of claim 7 derived from an immunogen in which Q is -O-, Y is -CH<2> - , X is carboxy and the accm is keyhole limpet haemocyanin which has an IC<50> of <1.00 ng/ml to MT-45 as calculated from a standard curve using MT-45-CME-HCTL-maleimide-HRP (Tracer 2) as detecting agent.
number: 9
text: An immunoassay method of detecting or determining MT-45 in anin vitro sample or in a solution comprising contacting the sample or solution with a detecting agent and an antibody of claims 7 or 8, detecting the bound detecting agent and deducing the presence or amount of MT-45.
number: 10
text: The immunoassay method of claim 9 in which the detecting agent is
number: 11
text: The immunoassay method of claim 10 in which R of the detecting agent is attached to the meta position of the phenyl ring.
number: 12
text: A detecting agent of structure
number: 13
text: The detecting agent of claim 12 which has the structure
Office action: search_report, dated 2017-12-06
Rejection reasons: inventive_step_epc_56
Prior art cited: ref_id: - PAPSUN ET AL.: "Analysis of MT-45, a novel synthetic opioid, in human whole blood by LC-MS-MS and its identification in a drug-related death", JOURNAL OF ANALYTICAL TOXICOLOGY, vol. 40, 2016, pages 313 - 317, XP002774046, DOI: doi:10.1093/jat/bkw012
ref_type: npl
severity: background
ref_id: - "Thermo Scientific Crosslinking Technical Handbook", April 2009
ref_type: npl
severity: background
ref_id: - "Bioconjugate Techniques", 1996, ACADEMIC PRESS, pages: 785
ref_type: npl
severity: background
ref_id: EP2679241A1
ref_type: patent
severity: background
ref_id: - PAPSUN DONNA ET AL: "Analysis of MT-45, a Novel Synthetic Opioid, in Human Whole Blood by LC-MS-MS and its Identification in a Drug-Related Death.", JOURNAL OF ANALYTICAL TOXICOLOGY MAY 2016, vol. 40, no. 4, 1 May 2016 (2016-05-01), pages 313 - 317, XP002774046, ISSN: 1945-2403
ref_type: npl
severity: background
ref_id: EP2679241
ref_type: patent
severity: obviousness
ref_id: - PAPSUN DONNA ET AL, "Analysis of MT-45, a Novel Synthetic Opioid, in Human Whole Blood by LC-MS-MS and its Identification in a Drug-Related Death.", JOURNAL OF ANALYTICAL TOXICOLOGY MAY 2016, (20160501), vol. 40, no. 4, ISSN 1945-2403, pages 313 - 317, XP002774046 [YD] 1-14 * abstract * * page  313, column  r, paragraph  3  - page  314, column  l, paragraph  5 * * page  315, column  l, paragraph  l  - column  r, paragraph  1 *
ref_type: npl
severity: obviousness
Final disposition: pending (2017-12-06)