Patent application EP16305658A (EP), filed 2016-06-06
Publication number: EP3255056A1
Title: METHOD FOR PRODUCING SELF-ASSEMBLYING PARAMYXOVIRAL NUCLEOCAPSID-LIKE PARTICLES AND THEIR USES
Abstract: The invention pertains to the domain of virology of Paramyxoviruses. The invention concerns a method for producing self-assembling paramyxoviral particles and a method for identifying a compound able to inhibit the replication or the transcription of a Paramyxovirus. The invention also pertains to the nucleocapsid-like particles obtainable by the method of the invention and their use for biotechnological and pharmaceutical applications.
IPC codes: A61K  39/   165            A I
C07K  14/    12            A I
C12N   7/    04            A I
Claims: number: 1
text: Method for producing self-assembling paramyxoviral nucleocapsid-like particles comprising the steps of :
number: 2
text: The method of claim 1 wherein step (a) further comprises the purification of N°P complexes.
number: 3
text: The method of claim 1 or 2 wherein the N and P peptides correspond to either:
number: 4
text: Nucleocapsid-like viral particle derived from a Paramyxovirus comprising a RNA of interest, wherein the nature of RNA is controlled.
number: 5
text: The particle of claim 3, wherein said particle comprises:
number: 6
text: The particle of claim 3 or 4 wherein the N peptide comprises a disordered C-terminal domain which is functionalized.
number: 7
text: A nucleocapsid-like particle obtainable by the method any one of claims 1 to 3 or as defined in any one of claims 4 to 6, for use as a medicament.
number: 8
text: The particle of claim 7, for use according to claim 7, wherein said particle is used to raise antibodies for vaccine or for an antiviral drug development.
number: 9
text: Method for identifying a compound able to inhibit the replication or transcription of a Paramyxovirus, wherein such compound is identified by its ability to abrogate the assembly of the NC-like particles.
number: 10
text: Method for identifying a compound able to inhibit the replication or transcription of a Paramyxovirus comprising the steps of:
number: 11
text: The method of claim 10 wherein step (a) further comprises the purification of N°P complexes.
number: 12
text: The method of claim 10 or 11 wherein the step e) of identification of the compound is performed using a fluorescent read-out assay, in particular a high-throughput assay.
Office action: search_report, dated 2017-12-13
Rejection reasons: novelty_epc_54
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* figure 2 *
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* figure 2 *
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ref_id: - SERGEY G. GURYANOV ET AL: "Crystal Structure of the Measles Virus Nucleoprotein Core in Complex with an N-Terminal Region of Phosphoprotein", JOURNAL OF VIROLOGY., vol. 90, no. 6, 15 March 2016 (2016-03-15), US, pages 2849 - 2857, XP055317491, ISSN: 0022-538X, DOI: 10.1128/JVI.02865-15
ref_type: npl
severity: background
ref_id: - FILIP YABUKARSKI ET AL: "Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone", NATURE STRUCTURAL & MOLECULAR BIOLOGY, NATURE PUBLISHING GROUP, US, vol. 21, no. 9, 1 September 2014 (2014-09-01), pages 754 - 759, XP002735220, ISSN: 1545-9985, [retrieved on 20140810], DOI: 10.1038/NSMB.2868
ref_type: npl
severity: background
ref_id: - J. CHAPMAN ET AL: "RSV604, a Novel Inhibitor of Respiratory Syncytial Virus Replication", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 51, no. 9, 1 September 2007 (2007-09-01), US, pages 3346 - 3353, XP055302638, ISSN: 0066-4804, DOI: 10.1128/AAC.00211-07
ref_type: npl
severity: background
ref_id: - SPEHNER D ET AL, "The Assembly of the Measles Virus Nucleoprotein into Nucleocapsid-like Particles Is Modulated by the Phosphoprotein", VIROLOGY, ELSEVIER, AMSTERDAM, NL, (19970609), vol. 232, no. 2, doi:10.1006/VIRO.1997.8568, ISSN 0042-6822, pages 260 - 268, XP004452233 [X] 4-6 * section "Nucleocapsid-like particles encapsidate RNA";; figures 3C,6 *
ref_type: npl
severity: novelty_destroying
ref_id: - R. COX ET AL, "Characterization of a Mumps Virus Nucleocapsidlike Particle", JOURNAL OF VIROLOGY., US, (20091101), vol. 83, no. 21, doi:10.1128/JVI.00504-09, ISSN 0022-538X, pages 11402 - 11406, XP055317465 [X] 4-6 * see the whole document, in particular the cited passages;; figures 2A,3 *
ref_type: npl
severity: novelty_destroying
ref_id: - JUOZAPAITIS M ET AL, "Generation of Sendai virus nucleocapsid-like particles in yeast", VIRUS RESEARCH, AMSTERDAM, NL, vol. 108, no. 1-2, ISSN 0168-1702, (20050301), pages 221 - 224, (20050301), XP027663813 [X] 4-6 * page  223, column  l, paragraph  l  - column  r, paragraph  1; figures 2,3 *
ref_type: npl
severity: novelty_destroying
ref_id: - SERGEY G. GURYANOV ET AL, "Crystal Structure of the Measles Virus Nucleoprotein Core in Complex with an N-Terminal Region of Phosphoprotein", JOURNAL OF VIROLOGY., US, (20160315), vol. 90, no. 6, doi:10.1128/JVI.02865-15, ISSN 0022-538X, pages 2849 - 2857, XP055317491 [A] 1-12 * the whole document *
ref_type: npl
severity: background
ref_id: - FILIP YABUKARSKI ET AL, "Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone", NATURE STRUCTURAL & MOLECULAR BIOLOGY, NATURE PUBLISHING GROUP, US, vol. 21, no. 9, doi:10.1038/NSMB.2868, ISSN 1545-9985, (20140901), pages 754 - 759, (20140810), XP002735220 [AD] 1-12 * the whole document *
ref_type: npl
severity: background
ref_id: - J. CHAPMAN ET AL, "RSV604, a Novel Inhibitor of Respiratory Syncytial Virus Replication", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, US, (20070901), vol. 51, no. 9, doi:10.1128/AAC.00211-07, ISSN 0066-4804, pages 3346 - 3353, XP055302638 [A] 9-12 * the whole document *
ref_type: npl
severity: background
Final disposition: pending (2017-12-13)