Patent application EP16305140A (EP), filed 2016-02-05
Publication number: EP3202780A1
Title: FUSION PROTEIN COMPRISING THE N PROTEIN OF A VIRUS OF THE PNEUMOVIRINAE SUBFAMILY
Abstract: The invention relates to a fusion protein comprising a N protein from a virus of the  Pneumovirinae  subfamily,  Pneumovirinae  subfamily, useful in immunization and vaccination methods.
IPC codes: A61K  39/   155            A I
C07K  14/   135            A I
C12N  15/    62            A I
C12N  15/    63            A I
C12N   5/    10            A I
Claims: number: 1
text: A fusion protein comprising a N protein from a virus of thePneumovirinae subfamily and a peptide of interest, said peptide of interest being inserted in frame at the loop of the β hairpin of the N terminal domain of the N protein in the region corresponding to residues 103-109 of human RSV N protein represented by SEQ ID NO: 1.
number: 2
text: The fusion protein according to claim 1, wherein the virus of thePneumovirinae subfamily is human or bovine respiratory syncytial virus.
number: 3
text: The fusion protein according to any one of claims 1 to 2, wherein the N protein is a native protein or a N protein modified in the region defined by the last 25 C-terminal amino acids, said modified N protein retaining the ability to interact with the P protein.
number: 4
text: The fusion protein according to any one of claims 1 to 3, wherein the peptide of interest is an antigen, preferably an epitope, more preferably a conformational epitope.
number: 5
text: The fusion protein according to claim 4, wherein the peptide of interest is an epitope derived from the F protein from a virus of thePneumovirinae subfamily, preferably from RSV.
number: 6
text: A complex comprising a fusion protein according to any one of claims 1 to 5 and a P protein, wherein the N and the P protein are from the same virus of thePneumovirinae subfamily.
number: 7
text: A nucleic acid coding for a fusion protein as defined in any one of claims 1 to 5.
number: 8
text: An expression vector comprising a nucleic acid as defined in claim 7 and preferably further comprising a nucleic acid coding for a P protein from the same virus of thePneumovirinae subfamily as the N protein of the fusion protein.
number: 9
text: A host cell containing:
number: 10
text: A method for producing a fusion protein according to any one of claims 1 to 5, said method comprising the culture of a host cell as claimed in claim 9 under conditions permitting the expression of fusion protein, and optionally the purification of the expressed fusion protein.
number: 11
text: A method for preparing fusion protein / P protein complexes according to claim 6, said method comprising the steps consisting in:
number: 12
text: A method for preparing a fusion protein according to any one of claims 1 to 5, said method comprising the separation of the protein fusion starting from the complex according to claim 6.
number: 13
text: A pharmaceutical composition comprising a fusion protein as defined in any one of claims 1 to 5 and/or a complex as defined in claim 6, and a pharmaceutically acceptable carrier, in which the pharmaceutical composition is preferably an immunogenic or vaccinal composition.
number: 14
text: The fusion protein according to any one of claims 1 to 5 or the complex as defined in claim 6, for use as a vaccine.
number: 15
text: The fusion protein or the complex for its use according to claim 14, wherein the peptide of interest of the fusion protein is an epitope derived from the RSV F protein, for use for preventing bronchiolitis or pneumonia.
Office action: search_report, dated 2017-08-09
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Final disposition: pending (2017-08-09)