Patent application EP16173776A (EP), filed 2016-06-09
Publication number: EP3254690A1
Title: A MONOCLONAL ANTIBODY INHIBITING IMMUNOSUPPRESSIVE FUNCTIONS OF HELICOBACTER PYLORI, ANTIGEN-BINDING FRAGMENT THEREOF, AND HYBRIDOMAS PRODUCING SUCH ANTIBODY
Abstract: The present invention relates to a monoclonal antibody that inhibits immunosuppressive functions of pathogens, antigen-binding fragment thereof, and hybridomas producing such antibody. The monoclonal antibody or antigen-binding fragment thereof bind to a peptide consisting an amino acid sequence represented by MEKVGKDGVITVE (SEQ ID NO: 1). The present invention also discloses use of the invented monoclonal antibody or antigen-binding fragment thereof, and method of preparation for such hybridomas.
IPC codes: A61K  39/    00            A I
A61P   1/    04            A I
C07K  16/    12            A I
Claims: number: 1
text: A monoclonal antibody binding to a peptide comprising an amino acid sequence represented by MEKVGKDGVITVE (SEQ ID NO 1) or similar expression thereto.
number: 2
text: The monoclonal antibody of claim 1, recognizing a peptide comprising an amino acid sequence represented by MEKVGKDGVITVE (SEQ ID NO 1).
number: 3
text: The monoclonal antibody of claim 1 or 2, comprising in a variable region of a light chain: CDR1 comprising an amino acid sequence represented by ASQSVDYDGDVFL (SEQ ID NO 2) CDR2 comprising an amino acid sequence represented by YAASN (SEQ ID NO 3), and CDR3 comprising an amino acid sequence represented by QSNEVPWT (SEQ ID NO 4).
number: 4
text: The monoclonal antibody of claim 3, comprising in a variable region of a light chain an amino acid sequence represented by positions 21-131 of SEQ ID NO 6.
number: 5
text: The monoclonal antibody of claim 1 or 2, comprising in a variable region of a heavy chain: CDR1 comprising an amino acid sequence represented by GYNMN (SEQ ID NO 7), CDR21 comprising an amino acid sequence represented by NINPYYGSTSYNQKFKG (SEQ ID NO 8), and CDR3 comprising an amino acid sequence represented by SPYYSNYWRYFDY (SEQ ID NO 9).
number: 6
text: The monoclonal antibody of claim 5, comprising in a variable region of a light chain an amino acid sequence represented by positions 20-141 of SEQ ID NO 11.
number: 7
text: The monoclonal antibody of claim 1 or 2, wherein the antibody is one selected from the group consisted of a chimerism, a humanized antibody and a human antibody.
number: 8
text: The monoclonal antibody of claim 1 or 2, wherein the antibody is produced by a hybridoma with a deposition number of ATCC Designation: PTA-122900.
number: 9
text: The monoclonal antibody of claim 1 or 2, wherein the antibody comprises an antigen-binding fragment selected from the group consisted of Fab, Fab', (Fab') 2, Fv and scFv.
number: 10
text: The monoclonal antibody of claim 1 or 2, comprising an isotype of immunoglobulin selected from the group consisted of IgG1, IgG2, IgG4, IgA, IgE and IgD.
number: 11
text: An antigen-binding fragment of the monoclonal antibody of any of claims 1-10.
number: 12
text: A hybridoma with a deposition number of ATCC Designation: PTA-122900.
number: 13
text: Use of the monoclonal antibody of any of claims 1-10 or the antigen-binding fragment of claim 11, as functional inhibitor to inhibit immunosuppressive functions of a pathogen.
number: 14
text: A pharmaceutical composition, comprising an effective dose of a monoclonal antibody of any of claims 1-10 or an antigen-binding fragment of claim 11.
number: 15
text: Use of the pharmaceutical composition of claim 15 as functional inhibitor to inhibit immune suppression.
number: 16
text: A hybridoma, for producing the monoclonal antibody of any of claims 1-10 or the antigen binding fragment of claim 11.
number: 17
text: Use of the monoclonal antibody of any of claims 1-10 or the antigen binding fragment of claim 11 in the preparation of a pharmaceutical composition.
number: 18
text: Method for preparation of a hybridoma, comprising the steps of: using a peptide composed of an amino acid sequence represented by MEKVGKDGVITVE (SEQ ID NO 1) as an antigen to cause a mammal to generates an immune response to the antigen; obtaining an immune cell from the mammal immunized against the pathogens and fusing the obtained immune cell with a mammalian myeloma cell to produce a hybridoma; cloning the obtained hybridoma to obtain a desired hybridoma
number: 19
text: Method for preparation of a monoclonal antibody, comprising the steps of: using the hybridoma obtained in accordance with claim 18 to produce antibodies; and harvesting the antibodies produced by the hybridoma.
Office action: search_report, dated 2017-12-13
Prior art cited: ref_id: US6403099B1
ref_type: patent
severity: background
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ref_id: - HSU WEI-TUNG: "Helicobacter pylori-derived heat shock protein 60 increases the induction of regulatory T cells (P3365)", THE JOURNAL OF IMMUNOLOGY, 1 May 2013 (2013-05-01), XP055291633, Retrieved from the Internet <URL:http://www.jimmunol.org/content/190/1_Supplement/202.5.short> [retrieved on 20160727]
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severity: background
ref_id: - HIROYUKI YAMAGUCHI ET AL, "Growth Inhibition of Helicobacter pylori by Monoclonal Antibody to Heat-Shock Protein 60", MICROBIOLOGY AND IMMUNOLOGY, JP, (19971214), vol. 41, no. 12, doi:10.1111/j.1348-0421.1997.tb01949.x, ISSN 0385-5600, pages 909 - 916, XP055292065 [I] 1-19 * figures 5-7 *
ref_type: npl
severity: background
ref_id: - SHIGERU KAMIYA ET AL, "Immune response to heat shock protein of Helicobacter pylori -a candidate as a vaccine component", KEIO JOURNAL OF MEDICINE., JP, (20020101), vol. 51, no. supplement2, doi:10.2302/kjm.51.supplement2_24, ISSN 0022-9717, pages 24 - 25, XP055292141 [I] 1-19 * page  25, column  l, paragraph  1; table 2 *
ref_type: npl
severity: background
ref_id: - OKADA ET AL, "Antibodies against heat shock protein 60 derived from Helicobacter pylori: Diagnostic implications in cardiovascular disease", JOURNAL OF AUTOIMMUNITY, LONDON, GB, (20070914), vol. 29, no. 2-3, doi:10.1016/J.JAUT.2007.05.004, ISSN 0896-8411, pages 106 - 115, XP022245355 [I] 1-19 * figures 1, 6 *
ref_type: npl
severity: background
ref_id: - KAO J Y ET AL, "Helicobacter pylori Immune Escape Is Mediated by Dendritic CellInduced Treg Skewing and Th17 Suppression in Mice", GASTROENTEROLOGY, ELSEVIER, AMSTERDAM, NL, vol. 138, no. 3, doi:10.1053/J.GASTRO.2009.11.043, ISSN 0016-5085, (20100301), pages 1046 - 1054, (20091118), XP026927461 [A] 1-19 * figure 6E *
ref_type: npl
severity: background
ref_id: - MIN ZHANG ET AL, "Helicobacter pylori-Pulsed Dendritic Cells Induce H. pylori-Specific Immunity in Mice", HELICOBACTER, US, (20080606), vol. 13, no. 3, doi:10.1111/j.1523-5378.2008.00606.x, ISSN 1083-4389, pages 200 - 208, XP055291850 [A] 1-19 * figure 5 *
ref_type: npl
severity: background
ref_id: - Hsu Wei-Tung, "Helicobacter pylori-derived heat shock protein 60 increases the induction of regulatory T cells (P3365)", The Journal of Immunology, (20130501), URL: http://www.jimmunol.org/content/190/1_Supplement/202.5.short, (20160727), XP055291633 [A] 1-19 * the whole document *
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Final disposition: pending (2017-12-13)