Patent application EP16000165A (EP), filed 2016-01-25
Publication number: EP3196209A1
Title: A MACADAMIA ALLERGEN
Abstract: The present invention relates to a polypeptide comprising a macadamia protein selected from the group comprising a protein running in a 2D SDS PAGE at pH 6.3 to 8.7 and 53 to 67 kDa and a protein running in a 2D SDS PAGE at pH 6.5 to 7.9 and 20 to 25 kDa or an antigenic variant thereof, preferably the protein running at pH 6.3 to 8.7 and 53 to 67 kDa or an antigenic variant thereof.
IPC codes: A61K  39/    35            A I
C07K  14/   415            A I
G01N  33/    68            A I
Claims: number: 1
text: A polypeptide comprising a macadamia protein
selected from the group comprising a protein running in a 2D SDS PAGE at pH 6.3 to 8.7 and 63 to 67 kDa and a protein running in a 2D SDS PAGE at pH 6.5 to 7.9 and 20 to 25 kDa or a variant thereof,
preferably the protein running at pH 6.3 to 8.7 and 53 to 67 kDa or a variant thereof.
number: 2
text: The polypeptide according to claim 1, wherein the polypeptide is an isolated and/or recombinant polypeptide.
number: 3
text: The polypeptide according to one of claims 1 to 2, wherein the polypeptide is a fusion polypeptide, preferably comprising a tag for detection or purification.
number: 4
text: A nucleic acid encoding the polypeptide according to any of claims 1 to 3, preferably in a vector, more preferably in a vector in a cell.
number: 5
text: The polypeptide according to one of claims 1 to 3, wherein the polypeptide Is immobilized.
number: 6
text: A medical device comprising the polypeptide according to any of claims 1 to 3 or to claim 5, wherein the medical device is preferably a diagnostically useful carrier, more preferably selected from the group comprising a bead, a test strip, a microtiter plate, a blot, preferably from the group comprising western blot, line blot and dot blot, a glass surface, a slide, a biochip, a membrane, a microarray, an electrophoresis gel and a microtiter plate.
number: 7
text: A method comprising the step detecting in a sample, preferably from a mammal, more preferably a human, an antibody binding to the polypeptide according to claim 1, which method is preferably for the diagnosis of a nut allergy, more preferably for the diagnosis of a macadamia allergy.
number: 8
text: A method for the treatment of a disease comprising the step administrating to a subject or contacting with a sample from a subject the polypeptide according to one of claims 1 to 3,
wherein the disease is preferably a nut allergy, more preferably a tree nut allergy, most preferably a macadamia allergy.
number: 9
text: An antibody binding to a macadamia protein selected from the group comprising a protein running in a 2D SDS PAGE at pH 6.3 to 8.7 and 53 to 67 kDa and a protein running in a 2D SDS PAGE at pH 6.5 to 7.9 and 20 to 25 kDa thereof,
preferably the protein running at pH 6.3 to 8.7 and 53 to 67 kDa,
wherein optionally the antibody is in complex with said macadamia protein or a variant thereof.
number: 10
text: A test kit for the diagnosis of a disease, comprising the polypeptide according to any of claims 1 to 3 or 5 and optionally a means for detecting a complex comprising the polypeptide and the antibody according to claim 9.
number: 11
text: A method for isolating an antibody binding to a macadamia protein selected from the group comprising a protein running in a 2D SDS PAGE at pH 6.3 to 8.7 and 53 to 67 kDa and a protein running in a 2D SDS PAGE at pH 6.5 to 7.9 and 20 to 25 kDa or an antigenic fragment thereof, comprising the steps
number: 12
text: A pharmaceutical composition or vaccine comprising the polypeptide according to any of claims 1 to 3, optionally comprising in addition a pharmaceutically acceptable carrier.
number: 13
text: The method according to any of claims 8 or 12, wherein the sample is a bodily fluid comprising antibodies, preferably selected from the group comprising whole-blood, serum, cerebrospinal fluid and saliva, more preferably whole blood.
number: 14
text: The method according to one of claims 8, 12 and 13, the antibody according to claim 10 and the test kit according to claim 11, wherein the antibody is an IgE antibody.
number: 15
text: A use of the polypeptide according to any of claims 1 to 3 or 5 for the diagnosis of an allergy, preferably a nut allergy, more preferably a tree nut allergy, most preferably of a macadamia allergy.
Office action: search_report, dated 2017-07-26
Rejection reasons: novelty_epc_54
Prior art cited: ref_id: EP15075033A
ref_type: patent
severity: background
ref_id: WO2013041540A1
ref_type: patent
severity: background
ref_id: - PALLARES, D.E: "Allergy to macadamia nut", ANN ALLERGY ASTHMA LMMUNOL, vol. 85, no. 5, 2000, pages 385 - 386
ref_type: npl
severity: background
ref_id: - SICHERER, S.H.; TEUBER, S.S.: "Current approach to the diagnosis and management of adverse reactions to foods", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 114, no. 5, 2004, pages 1146 - 1150
ref_type: npl
severity: background
ref_id: - RANCE, F.; JUCHET, A.; BREMONT. F.; DUTAU, G.: "Correlation between skin prick tests using commercial extracts and fresh foods, specific IgE and food challenges", ALLERGY, vol. 52.10, 1997, pages 1031 - 1035
ref_type: npl
severity: background
ref_id: - VOLCHECK, G. W., CLINICAL ALLERGY, DIAGNOSIS AND MANAGEMENT, 2009
ref_type: npl
severity: background
ref_id: - CODREANU. F.; COLLIGNON, 0.; ROITEL, 0.; THOUVENOT, B.; SAUVAGE, C.; VLLALN, A.C.; COUSIN, M.O.; DECOSTER, A.; RENAUDIN, J.M.; AST: "A novel immunoassay using recombinant allergens simplifies peanut allergy diagnosis", INT ARCH ALLERGY,LMMUNOL, vol. 154, no. 3, 2011, pages 216 - 226
ref_type: npl
severity: background
ref_id: - GHORBANI, M.; MORGAN, M.R.A: "Protein characterisation and immunochemical measurements of residual macadamia nut proteins In foodstuffs", FOOD AGR IMMUNOT, vol. 21, 2010, pages 347 - 360
ref_type: npl
severity: background
ref_id: - HERBST, R.A.; WAHL, R.; FROSCH, P.J: "Specific IgE reactivity and identification of potential allergens in macadamia allergy", J EUR ACAD DERMATOL VENEREOL, vol. 24, no. 11, 2010, pages 1361 - 1363
ref_type: npl
severity: background
ref_id: - SATHE, S.K.; VENKATACHALAM, M.; SHARMA, G.M.; KSHIRSAGAR, H.H.; TEUBER, S.S.; ROUX, K.H.: "Solubilization and electrophoretic characterization of select edible nut seed proteins", J AGRIC FOOD CHEM, vol. 57, no. 17, 2009, pages 7846 - 7856
ref_type: npl
severity: background
ref_id: - RAOULT, D.; DASCH, G. A: "The line blot: an immunoassay for monoclonal and other antibodies. Its application to the serotyping of gram-negative bacteria", J. IMMUNOL. METHODS, vol. 126, no. 1-2, 1989, pages 57 - 65
ref_type: npl
severity: background
ref_id: - ARTHUR LESK: "Introduction to bioinformatics. 3rd ed.", 2008, OXFORD UNIVERSITY PRESS
ref_type: npl
severity: background
ref_id: - LARKIN, M. A.; BLACKSHIELDS, G.; BROWN, N. P.; CHENNA, R.; MCGETTIGAN, P. A.; MCWILLIAM, H.; VALENTIN, F.; WALLACE, I. M.; WILM, A: "Clustal W and Clustal X version 2.0", BIOINFORMATICS, vol. 23, 2007, pages 2947 - 2948
ref_type: npl
severity: background
ref_id: - SAMBROOK, J.; FRITSCH, E. F.; MANIATIS, T.: "Molecular Cloning", CSH
ref_type: npl
severity: background
ref_id: - BROWN T. A: "Gene Cloning - an introduction", 1986, CHAPMAN & HALL
ref_type: npl
severity: background
ref_id: - "Antibody Purification", GE HEALTHCARE LIFE SCIENCES, article "Strategies for Protein Purification"
ref_type: npl
severity: background
ref_id: - BURGESS, R. R.; DEUTSCHER, M. P.: "Guide to Protein Purification", 2009
ref_type: npl
severity: background
ref_id: - ZANE, H. D: "Immunology - Theoretical & Practical Concepts in Laboratory Medicine", 2001, W. B. SAUNDERS COMPANY
ref_type: npl
severity: background
ref_id: - "The Basophil Activation Test in Immediate-Type Drug Allergy", IMMUNOL. ALLERGY CLIN. N. AM., vol. 29, 2009, pages 555 - 566
ref_type: npl
severity: background
ref_id: - CANDIANO ET AL., ELECTROPHORESIS, vol. 25, no. 9, May 2004 (2004-05-01), pages 1327 - 1333
ref_type: npl
severity: background
ref_id: US2003124060A1
ref_type: patent
severity: background
ref_id: WO2004042026A2
ref_type: patent
severity: background
ref_id: - DATABASE Geneseq [online] 15 June 2007 (2007-06-15), "Macadamia integrifolia antimicrobial protein.", XP002758435, retrieved from EBI accession no. GSP:AAW62830 Database accession no. AAW62830
ref_type: npl
severity: background
ref_id: - KENNETH H. ROUX ET AL: "Tree Nut Allergens", INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY., vol. 131, no. 4, 20 August 2003 (2003-08-20), CH, pages 234 - 244, XP055277661, ISSN: 1018-2438, DOI: 10.1159/000072135
ref_type: npl
severity: background
ref_id: - BARRE ET AL: "Vicilin allergens of peanut and tree nuts (walnut, hazelnut and cashew nut) share structurally related IgE-binding epitopes", MOLECULAR IMMUNOLOGY, PERGAMON, GB, vol. 45, no. 5, 29 October 2007 (2007-10-29), pages 1231 - 1240, XP022402155, ISSN: 0161-5890, DOI: 10.1016/J.MOLIMM.2007.09.014
ref_type: npl
severity: background
ref_id: - BEATRIZ CABANILLAS ET AL: "Pine nut allergy: Clinical features and major allergens characterization", MOLECULAR NUTRITION & FOOD RESEARCH, vol. 56, no. 12, 19 December 2012 (2012-12-19), DE, pages 1884 - 1893, XP055277789, ISSN: 1613-4125, DOI: 10.1002/mnfr.201200245
ref_type: npl
severity: background
ref_id: - SHRIDHAR K. SATHE ET AL: "Solubilization and Electrophoretic Characterization of Select Edible Nut Seed Proteins", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 57, no. 17, 9 September 2009 (2009-09-09), US, pages 7846 - 7856, XP055277665, ISSN: 0021-8561, DOI: 10.1021/jf9016338
ref_type: npl
severity: background
ref_id: US2003124060
ref_type: patent
severity: background
ref_id: WO2004042026
ref_type: patent
severity: background
ref_id: - "Macadamia integrifolia antimicrobial protein.", Geneseq, (20070615), Database accession no. AAW62830, URL: EBI, XP002758435 [X] 1-4,9,11 * sequence . *
ref_type: npl
severity: novelty_destroying
ref_id: - KENNETH H. ROUX ET AL, "Tree Nut Allergens", INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY., CH, (20030820), vol. 131, no. 4, doi:10.1159/000072135, ISSN 1018-2438, pages 234 - 244, XP055277661 [I] 1-15 * Whole document, especially table 1 *
ref_type: npl
severity: background
ref_id: - BARRE ET AL, "Vicilin allergens of peanut and tree nuts (walnut, hazelnut and cashew nut) share structurally related IgE-binding epitopes", MOLECULAR IMMUNOLOGY, PERGAMON, GB, (20071029), vol. 45, no. 5, doi:10.1016/J.MOLIMM.2007.09.014, ISSN 0161-5890, pages 1231 - 1240, XP022402155 [I] 1-15 * the whole document *
ref_type: npl
severity: background
ref_id: - BEATRIZ CABANILLAS ET AL, "Pine nut allergy: Clinical features and major allergens characterization", MOLECULAR NUTRITION & FOOD RESEARCH, DE, (20121219), vol. 56, no. 12, doi:10.1002/mnfr.201200245, ISSN 1613-4125, pages 1884 - 1893, XP055277789 [I] 1-15 * the whole document *
ref_type: npl
severity: background
ref_id: - SHRIDHAR K. SATHE ET AL, "Solubilization and Electrophoretic Characterization of Select Edible Nut Seed Proteins", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, US, (20090909), vol. 57, no. 17, doi:10.1021/jf9016338, ISSN 0021-8561, pages 7846 - 7856, XP055277665 [AD] 1-15 * the whole document *
ref_type: npl
severity: background
Final disposition: pending (2017-07-26)