Patent application EP16165388A (EP), filed 2016-04-14
Publication number: EP3231419A1
Title: GENERATION OF DNA HYDROGELS FROM LINEAR BUILDING BLOCKS
Abstract: The present invention relates to a method for producing a DNA hydrogel as well as a DNA hydrogel obtained by the method and the use of such DNA hydrogel.
IPC codes: A61K   9/    16            A I
C12N  15/    10            A I
Claims: number: 1
text: Method for producing a DNA hydrogel comprising the following steps:
number: 2
text: Method according to claim 1, wherein the basis DNA is selected from the group consisting of plasmid DNA, Herring Sperm DNA, Salmon Sperm DNA and lambda phage DNA.
number: 3
text: Method according to claim 1 or 2, wherein the enzymatic digest is done with at least one restriction enzyme.
number: 4
text: Method according to at least one of the preceding claims, wherein the linear dsDNA building blocks have a length of at least 10 bp.
number: 5
text: Method according to at least one of the preceding claims, wherein the building blocks comprise at least one ssDNA overhang.
number: 6
text: Method according to claim 5, wherein the overhang comprises at least one nucleotide.
number: 7
text: Method according to at least one of the preceding claims, wherein the method further comprises the step of separating the obtained building blocks according to their length prior to assembly.
number: 8
text: Method according to at least one of the preceding claims, wherein the method further comprises the step of adding non-linear dsDNA building blocks prior to assembly.
number: 9
text: Method according to claim 8, wherein the non-linear dsDNA building blocks are selected from the group consisting of star-shaped dsDNA building blocks, T-shaped dsDNA building blocks, X-shaped dsDNA building blocks and Y-shaped dsDNA building blocks.
number: 10
text: Method according to claim 8 or 9, wherein the non-linear dsDNA building blocks are added in an amount of at most 10 wt.-% based on the weight of the linear dsDNA building blocks.
number: 11
text: Method according to at least one of the preceding claims, wherein enzymatic ligation is done with a DNA ligase.
number: 12
text: Method according to claim 11, wherein the DNA ligase is selected from the group consisting of T4 DNA ligase andE .coli DNA ligase.
number: 13
text: DNA hydrogel obtained by the method of at least one of the preceding claims, wherein the DNA hydrogel comprises assembled dsDNA building blocks and liquid medium.
number: 14
text: DNA hydrogel according to claim 13, wherein assembled dsDNA building blocks and liquid medium constitute at least 90 wt.-% of the total weight of the DNA hydrogel.
number: 15
text: Use of a DNA hydrogel according to claims 13 or 14 in applications selected from the group consisting of cell transplant therapy, bio-mineralization, tissue engineering, gene therapy, drug delivery, wound healing, cell culture, cell-free protein biosynthesis, bio-sensing, cosmetics and 3D printing.
Office action: search_report, dated 2017-10-18
Rejection reasons: novelty_epc_54
Prior art cited: ref_id: - LABEAN, NATURE MATERIALS, 2006, pages 767 - 768
ref_type: npl
severity: background
ref_id: - UM ET AL., NATURE MATERIALS, 2006, pages 797 - 801
ref_type: npl
severity: background
ref_id: - CHENG ET AL., ANGEWANDTE CHEMIE INTERNATIONAL EDITION, 2009, pages 7660 - 7663
ref_type: npl
severity: background
ref_id: - XING ET AL., ADVANCED MATERIALS, 2011, pages 1117 - 1121
ref_type: npl
severity: background
ref_id: - N611 ET AL., ANGEWANDTE CHEMIE INTERNATIONAL EDITION, 2014, pages 8328 - 8332
ref_type: npl
severity: background
ref_id: US2007148246A1
ref_type: patent
severity: background
ref_id: US2007117177A1
ref_type: patent
severity: background
ref_id: WO2010017264A2
ref_type: patent
severity: background
ref_id: JP2007187897A
ref_type: patent
severity: background
ref_id: - TANJA NÖLL ET AL: "Construction of Three-Dimensional DNA Hydrogels from Linear Building Blocks", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 53, no. 32, 25 June 2014 (2014-06-25), DE, pages 8328 - 8332, XP055308112, ISSN: 1433-7851, DOI: 10.1002/anie.201402497
ref_type: npl
severity: background
ref_id: - UM S H ET AL: "Enzyme-catalysed assembly of DNA hydrogel", NATURE MATERIALS, NATURE PUBLISHING GROUP, GB, vol. 5, no. 10, 1 October 2006 (2006-10-01), pages 797 - 801, XP002692004, ISSN: 1476-1122, [retrieved on 20060924], DOI: 10.1038/NMAT1741
ref_type: npl
severity: background
ref_id: - ENJUN CHENG ET AL: "A pH-Triggered, Fast-Responding DNA Hydrogel", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 48, no. 41, 28 September 2009 (2009-09-28), pages 7660 - 7663, XP055088426, ISSN: 1433-7851, DOI: 10.1002/anie.200902538
ref_type: npl
severity: background
ref_id: - YONGZHENG XING ET AL: "Self-Assembled DNA Hydrogels with Designable Thermal and Enzymatic Responsiveness", ADVANCED MATERIALS, vol. 23, no. 9, 22 December 2010 (2010-12-22), pages 1117 - 1121, XP055125813, ISSN: 0935-9648, DOI: 10.1002/adma.201003343
ref_type: npl
severity: background
ref_id: - KHIEM VAN NGUYEN ET AL: "Investigating DNA hydrogels as a new biomaterial for enzyme immobilization in biobatteries", CHEMICAL COMMUNICATIONS - CHEMCOM., vol. 51, no. 66, 1 January 2015 (2015-01-01), pages 13071 - 13073, XP055308143, ISSN: 1359-7345, DOI: 10.1039/C5CC04810A
ref_type: npl
severity: background
ref_id: - YOUNG HOON ROH ET AL: "Photocrosslinked DNA nanospheres for drug delivery", MACROMOLECULAR RAPID COMMUNICATIONS,, vol. 31, no. 13, 1 July 2010 (2010-07-01), pages 1207 - 1211, XP002692006, ISSN: 1022-1336, [retrieved on 20100525], DOI: 10.1002/MARC.200900872
ref_type: npl
severity: background
ref_id: US2007117177
ref_type: patent
severity: novelty_destroying
ref_id: US2007148246
ref_type: patent
severity: novelty_destroying
ref_id: JP2007187897
ref_type: patent
severity: background
ref_id: WO2010017264
ref_type: patent
severity: novelty_destroying
ref_id: - TANJA NÖLL ET AL, "Construction of Three-Dimensional DNA Hydrogels from Linear Building Blocks", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, DE, (20140625), vol. 53, no. 32, doi:10.1002/anie.201402497, ISSN 1433-7851, pages 8328 - 8332, XP055308112 [AD] 1-15 * the whole document *
ref_type: npl
severity: background
ref_id: - UM S H ET AL, "Enzyme-catalysed assembly of DNA hydrogel", NATURE MATERIALS, NATURE PUBLISHING GROUP, GB, vol. 5, no. 10, doi:10.1038/NMAT1741, ISSN 1476-1122, (20061001), pages 797 - 801, (20060924), XP002692004 [AD] 1-15 * the whole document *
ref_type: npl
severity: background
ref_id: - ENJUN CHENG ET AL, "A pH-Triggered, Fast-Responding DNA Hydrogel", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, (20090928), vol. 48, no. 41, doi:10.1002/anie.200902538, ISSN 1433-7851, pages 7660 - 7663, XP055088426 [AD] 1-15 * the whole document *
ref_type: npl
severity: background
ref_id: - YONGZHENG XING ET AL, "Self-Assembled DNA Hydrogels with Designable Thermal and Enzymatic Responsiveness", ADVANCED MATERIALS, (20101222), vol. 23, no. 9, doi:10.1002/adma.201003343, ISSN 0935-9648, pages 1117 - 1121, XP055125813 [AD] 1-15 * the whole document *
ref_type: npl
severity: background
ref_id: - KHIEM VAN NGUYEN ET AL, "Investigating DNA hydrogels as a new biomaterial for enzyme immobilization in biobatteries", CHEMICAL COMMUNICATIONS - CHEMCOM., (20150101), vol. 51, no. 66, doi:10.1039/C5CC04810A, ISSN 1359-7345, pages 13071 - 13073, XP055308143 [A] 1-15 * the whole document *
ref_type: npl
severity: background
ref_id: - YOUNG HOON ROH ET AL, "Photocrosslinked DNA nanospheres for drug delivery", MACROMOLECULAR RAPID COMMUNICATIONS,, vol. 31, no. 13, doi:10.1002/MARC.200900872, ISSN 1022-1336, (20100701), pages 1207 - 1211, (20100525), XP002692006 [A] 1-15 * the whole document *
ref_type: npl
severity: background
Final disposition: pending (2017-10-18)