Patent application EP15306887A (EP), filed 2015-11-27
Publication number: EP3173098A1
Title: IMMUNOSTIMULATORY COMPOSITIONS
Abstract: [0001]    The invention relates to the use of melanin, complexed with an antigen, as a immunostimulatory composition.
IPC codes: A61K  39/    00            A I
A61K  39/   385            A I
Claims: number: 1
text: An immunostimulatory composition comprising a melanin macromolecule complexed with an antigen.
number: 2
text: The immunostimulatory composition of claim 1, wherein the melanin is a soluble melanin.
number: 3
text: The immunostimulatory composition of claim 1 or 2, which is susceptible to be obtained by polymerizing melanin precursors in the presence of the antigen.
number: 4
text: The immunostimulatory composition of claim 3, wherein polymerization is performed by exposing the melanin precursors and the antigen to an oxidizing agent.
number: 5
text: The immunostimulatory composition of claim 3 or 4, wherein the antigen and the melanin precursor are covalenty linked prior to polymerization.
number: 6
text: The immunostimulatory composition of claim 3 or 4, wherein the antigen and the melanin precursor are not covalenty linked prior to polymerization.
number: 7
text: The immunostimulatory composition of any one of claims 3 to 6, wherein the melanin precursor is chosen in the group consisting of L-tyrosine, L-dopa, D-dopa, 6-hydroxy-Dopa, dopaquinone, cyclodopa, dopachrome, DHICA, DHI, dopamine-o-quinone, dopamine, leukodopaminochrome and dopaminochrome.
number: 8
text: The immunostimulatory composition of any one of claims 1 to 7, which also comprises an adjuvant.
number: 9
text: The immunostimulatory composition of claim 8, wherein the adjuvant is complexed with the melanin macromolecule complexed with an antigen.
number: 10
text: The immunostimulatory composition of claim 8, wherein the adjuvant is not complexed with the melanin macromolecule complexed with an antigen.
number: 11
text: The immunostimulatory composition of any one of claims 8 to 10, wherein the adjuvant is selected in the group consisting of alum, emulsions PRR ligands, TLR3 and RLR ligands, TLR4 ligands, TLR5 ligands, TLR7/8 ligands, TLR9 ligands, in particular poly I:C and CpG ODNs.
number: 12
text: The immunostimulatory composition of any one of claims 1 to 11, wherein the antigen comprises at least one epitope chosen in the group consisting of MHC epitopes and B-cell epitopes.
number: 13
text: Use of melanin or a melanin precursor for the preparation of an immunostimulatory composition intended to elicit a immune response against an antigen when administered to a host.
number: 14
text: Melanin or melanin precursor for its use for increasing or eliciting a immune response against a target antigen.
number: 15
text: Immunostimulatory composition comprising a melanin and a target antigen for its use as a vaccine to protect a animal against a disease implicating cells expressing the target antigen.
number: 16
text: A method for obtaining an immunostimulatory composition comprising the steps of
number: 17
text: The method of claim 16, wherein the antigen and the melanin precursor in the composition of step a) are covalenty bound.
number: 18
text: The method of claim 16, wherein the antigen and the melanin precursor in the composition of step a) are not covalenty bound.
number: 19
text: The method of any one of claims 16 to 18, wherein the melanin precursor is chosen in the group consisting of L-tyrosine, L-dopa, D-dopa, 6-hydroxy-Dopa, dopaquinone, cyclodopa, dopachrome, DHICA, DHI, dopamine-o-quinone, dopamine, leukodopaminochrome and dopaminochrome.
number: 20
text: The method of any one of claims 16 to 19, wherein the composition of a) also contains an adjuvant.
number: 21
text: The method of any one of claims 16 to 19, wherein an adjuvant is added to the composition obtained after step b).
Office action: search_report, dated 2017-05-31
Rejection reasons: novelty_epc_54
inventive_step_epc_56
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severity: background
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severity: obviousness
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ref_type: npl
severity: obviousness
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severity: obviousness
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severity: obviousness
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severity: background
Final disposition: pending (2017-05-31)