The Buffalo News, High Five cells, are commonly used for expression of recombinant proteins using baculovirus. Bacterial and yeast culture methods. For bacteria and yeasts, small quantities of cells are usually grown on a solid support that contains nutrients embedded in it, usually a gel such as agar, while large-scale cultures are grown with the cells suspended in a nutrient broth. Viral culture methods. The culture of viruses requires the culture of cells of mammalian, plant, fungal or bacterial origin as hosts for the growth and replication of the virus. Whole wild type viruses, recombinant viruses or viral products may be generated 	Cell culture
19701892	in cell types other than their natural hosts under the right conditions. Depending on the species of the virus, infection and viral replication may result in host cell lysis and formation of a viral plaque. Cell culture Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies the essential nutrients (amino 	Cell culture
19701893	Costa di Mezzate Costa di Mezzate Costa di Mezzate (Bergamasque: ) is a comune in the province of Bergamo, in Lombardy, Italy. Neighbouring communes are Albano Sant'Alessandro, Bagnatica, Bolgare, Calcinate, Gorlago and Montello. Costa di Mezzate Costa di Mezzate (Bergamasque: ) is a comune in the province of Bergamo, in Lombardy, Italy. Neighbouring communes are Albano Sant'Alessandro, Bagnatica, Bolgare, Calcinate, Gorlago and Montello. 	Costa di Mezzate
19701894	The Buffalo News The Buffalo News The Buffalo News is the daily newspaper of the Buffalo–Niagara Falls metropolitan area, located at 1 News Plaza in downtown Buffalo, New York. It was for decades the only paper fully owned by Warren Buffett's Berkshire Hathaway. On January 29, 2020, the paper reported that it was being sold to Lee Enterprises. History. The News" was founded in 1873 by Edward Hubert Butler, Sr. as a Sunday paper. On October 11, 1880, it began publishing daily editions as well, and in 1914, it became an inversion of its original existence by publishing Monday to Saturday , with no publication on Sunday. During most of its life, the "News" was known as "The Buffalo Evening News". A gentleman's agreement between the "Evening News" and the "Buffalo Courier-Express" meant that the "Evening News" would be evening-only, and the "Courier-Express" would be morning-only. Until 1977, the "News" did not publish on Sundays because of the agreement, and its weekend edition appeared on Saturday evening. The Butler family owned the "Evening News" until 1977, when longtime owner and publisher Katherine Butler, granddaughter of the founder, died and left no heirs. The "Evening News" properties were  placed in a blind trust, which sold the "Evening News" to Berkshire Hathaway. The new owners began publishing on Saturday and Sunday mornings. After a period of financial decline, the "Courier-Express" published its last issue on September 19, 1982. The "Evening News" then shortened its name to "The Buffalo News" and became an all-day newspaper, publishing two editions seven days a week. On October 1, 2006, the "News" announced it would abandon its evening edition later that month. "The Buffalo News" had published three morning editions (Western New York, Final and Niagara) that appear online at BuffaloNews.com, reaching  over 400,000 readers, across eight counties each day. These separate editions were eliminated in 2018 and consolidated into a single Final edition, in response to a newsprint shortage. "The News" Designated Market Area had the largest adult population in upstate New York. Counties in total circulation area: New York - Allegany, Cattaraugus, Chautauqua, Chemung, Erie, Genesee, Livingston, Monroe, Niagara, Ontario, Steuben, Wyoming; Pennsylvania - Cameron, Erie, McKean, Potter, Warren. The newspaper founded and owned the WBEN television and radio stations, which are now WIVB (Channel 4), WBEN (930), WYRK (106.5) and WTSS (102.5), respectively. The radio stations are now owned by separate  companies (WBEN and WTSS are now held by Entercom; WYRK by Townsquare Media), but in 2014, WIVB came back under partial coownership, with the "News" when Buffett's Media General merged with the WIVB parent company, LIN Media. The online version of "The Buffalo News" operates under a soft paywall allowing a limited number of page views per week. All Buffalo Bills-related content, branded as "BN Blitz", is behind a hard paywall. On January 29, 2020, the "News" reported it was being sold along with the rest of Berkshire Hathaway's newspaper portfolio to Lee Enterprises, an Iowa-based  owner of 50 newspapers that has had significant ties to Berkshire Hathaway since 2012 and had operated Berkshire Hathaway's newspapers since 2018. Pulitzer Prizes. Journalists for "The Buffalo News" and "The Buffalo Evening News" have won four Pulitzer Prizes: "News" journalists have been finalists for three other Pulitzer Prizes, but did not win: Other journalists who won awards include Richard J. Burke, who in 1972 won the New York State Associated Press Award for his series of articles about bicycling around Western New York. Past publishers and editors. Only three members of the Butler family were publishers. The Buffalo