Johan Sebastiaan Ploem
Johan Sebastiaan Ploem HonFRMS (born 25 August 1927, Sawahlunto) is a Dutch microscopist and digital artist, who made significant contribution to the field of fluorescence microscopy. Personal life. Ploem was born on Sumatra, then part of the Dutch East Indies. He started painting as a small boy and was educated in drawing and painting in Maastricht, the Netherlands. Career. Ploem received his education at the University of Utrecht in the Netherlands, Harvard University and the University of Amsterdam. He has since then been employed by a number of academic institutions, including the University of Miami, Harvard, the  University of Amsterdam, and the University of Leiden, where he served as a professor at the Faculty of Medicine. He also cooperated with industry, in particular in the branch of optics and concentrated on research in image analysis, participating in a project aiming to automate cancer cell recognition. Ploem's prototype fluorescence epi-illuminators and microscopes form a part of the permanent exposition of the Dutch National Museum for the history of Science and Medicine. Ploem described a new sub-category of digital art. Transforming digital algorithms were used to create novel pictorial handwritings for digital painting. Ploem is Professor  Emeritus at Leiden University, the Netherlands. He is a graduate of Utrecht University, the Netherlands, receiving an MD in 1972. He worked as Intern in the Broussais Hospital (Paris, France) with Professor Pasteur Valery-Radot. In 1963, Dr. Ploem was elected a Fulbright Fellow for Study at the Harvard University School of Public Health, receiving a Master of Public Health degree Cum Laude in 1954. He obtained a Ph.D. degree in 1967 from the University of Amsterdam, the Netherlands. Professor Ploem has served as visiting lecturer or professor at various universities: Dundee. Scotland; University of Florida, USA; Monash University, Melbourne , Australia; University of Beijing, China; and at the Free University of Brussels, Belgium. In 1980, he was appointed to a professorship in the Department of Cytochemistry and Cytometry at Leiden University. He retired from that position in 1992. Numerous honors and awards have been bestowed on Professor Ploem. In 1976 he was elected to the honorary Fellowship of the Royal Microscopical Society, Oxford England. In 1977 he received a Fellowship to the Papanicolaou Cancer Research Institute in Miami, Florida, and in 1979 a Fellowship to the Institute for Cell Analysis at the University of Miami, Florida. In 1982, he was  the co-recipient of the C. E. Alken Foundation Award, Switzerland. In 1993, he was elected as the first Honorary Member of the International Society for Analytical Cytology. In 1993, Professor Ploem held the Erica Wachtel Medal Lecture at the British Society for Clinical Cytology meeting. In 1994, the European Society for Analytical Cellular Pathology established a Conference Keynote “Ploem” Lecture for invited scientists at its future general meetings. The International Society of Analytical Cytology invited Professor Ploem to present its inaugural “Robert Hooke” lecture. In 1995, he was invited by the Royal Microscopical Society to give the inaugural CYTO  lecture. Fluorescence microscopy. Around 1962 Ploem started work in collaboration with Schott on the development of dichroic beam splitters for reflection of blue and green light for fluorescence microscopy using epi illumination. At the time of his first publication on fluorescence microscopy using epi illumination with narrow-band blue and green light, he was not aware of the development of a dichroic beam splitter for UV excitation with incident light by Brumberg and Krylova. Digital painting. Bas Ploem started painting as a small boy. While still at secondary school he attended an evening course in drawing and painting at the  “Kunstnijverheidsschool Maastricht”. Ploem's presence in Paris was important for his knowledge and interest in art since he could regularly visit his cousins in Paris, the painter Frits Klein and his son Yves. He visited the Kleins when Yves was making his first monochromes. In the last years of his activities at the faculty of medicine at Leiden University, he concentrated on research in image analysis. He was asked to participate in a European project with the aim of automating cancer cell recognition using computer analysis. It concerned a collaborative project with the German optical company Leitz/Leica Microsystems, and  the Institute for Mathematical Morphology in Fontainebleau, France. Together with a team, Professor Jean Serra at this institute had developed an image analysis method, now internationally known as ‘Mathematical Morphology’ (MM). With his experience as an analogue painter, Ploem saw the possibility of also applying the methods of mathematical morphology to the creation of digital art . At the International Symposium on Mathematical Morphology in Amsterdam (1998), J.S Ploem presented a paper on the creation of computer graphics with Mathematical Morphology, using for the first time, the transforming algorithms from the Fontainebleau group for the creation of digital art. (Mathematical Morphology  and its Applications to Image and Signal Processing, Heijmans and Roerdink (Eds), Kluwer. 1098: page 355:) Ploem described and developed methodology for the creation of transformational digital art. Mountain flowers as the first topic for digital image analysis His first digital graphics of nature scenes were shown in his exposition at a regional art centre in the Pyrenees (Ossega, June, 1997). Ploem was probably the first person to systematically use mathematical morphology for the creation of digital art, Ploem's work attracted international attention and he was invited as a plenary speaker at an international mathematical conference in Amsterdam in  1998 to explain his new type of digital art. He also received an invitation to show his art work in an exposition at this conference. The organisers of this meeting asked Ploem to write a chapter on his novel technique for digital art in a book (Kluwer, ) that was published on the occasion of this meeting. He was invited for a symposium on ‘Art et Science’ at the University of Caen, France (April, 2001). At the art exposition connected with this symposium, he presented 6 digital graphics that were dominated by chaotic transformations of rock art themes. A similar invitation  was made by the University of Basel in Switzerland (April, 2002).