Australian Dung Beetle Project
The Australian Dung Beetle Project (1965–1985), conceived and led by Dr George Bornemissza of the Commonwealth Scientific and Industrial Research Organisation (CSIRO), was an international scientific research and biological control project with the primary goal to control the polluting effects of cattle dung. Background and inception. Upon his arrival to Australia from Hungary in 1951, Dr Bornemissza, an entomologist and ecologist, noted that Australian farmland was covered in a large number of cattle dung pads. This was in contrast to the fields of Europe where the dung was removed and recycled back into the soil by  various species of dung beetle (coprids). Native Australian species of beetle had co-evolved alongside marsupials such as the kangaroo and wombat, which produce small, hard, dry and fibrous pellets of dung. Cattle were relatively recently introduced to Australia by European settlers in the 1880s and produce large, soft, moist dung pads. Native beetles, with a few exceptions, are not adapted to utilise this type of dung as a food source or breeding ground and so without such fauna, the dung pads remain on the pasture and take months or even years to decompose. Cattle will not feed from the  area of rank pasture surrounding the dung pad, and with the large quantity of dung produced (up to 12 pads per animal per day), this reduces the area of land available for cattle grazing by as much as per year. Cattle dung is also a primary breeding ground for several pestilent species of fly and parasitic worm. Bornemissza suggested in 1960 that the introduction to Australia of foreign dung beetle species, which had co-evolved alongside bovines and large herbivores, would be beneficial in removing the dung, thus improving cattle grazing and nutrient recycling and reducing the number of flies  and worms. Aims of the project. The overall aim of the Australian Dung Beetle Project was to establish a "minifauna", that is, a subsection of the natural dung beetle fauna, of introduced dung beetles on the Australian mainland and in Tasmania. Once introduced, dung beetles in Australia were studied in order to determine their effects on: Care had to be taken to introduce only those species that would be most compatible with the Australian climates and soil types, that were not under great threat of predation or of themselves becoming pests, and that effectively dispersed dung pads within an ideal  time frame of 48 hours so as to minimise successful fly and worm breeding by disrupting their reproductive cycles. Quarantine. It was estimated that as many as 160 species of dung beetle would need to be imported into Australia in order to establish a minifauna of beetles that would be suitably adapted for the different Australian climates and soil types. However, it was also important that no other potential pests "piggy-backed" their way to Australia with the beetles. Therefore, strict quarantine measures were established to ensure no pestilent species made their way to Australia. The first beetles to be  imported to Australia came from Hawaii. Here, dung beetles, particularly the species "Onthophagus gazella", had already been successfully introduced from Africa in order to help biologically control the numbers of horn fly. It was thought that these beetles would be a safe source since the only parasite present in Hawaii that does not already exist in Australia is the giant liver fluke, "Fasciola gigantean", the eggs of which do not survive ingestion by beetles. Samples of "O. gazella" were shipped to the CSIRO research base in Canberra, where they were kept under quarantine and observed before potential release. Unfortunately, direct  importation from Hawaii was found to be unviable since it was discovered that the beetles were infested with potentially harmful pyemotid mites. For this reason, these particular beetles were never released onto Australian pastures. However, the mite-infested beetles were not destroyed and were instead used to breed new generations of beetles under sterile conditions. Dung beetle eggs were dipped in 3% formalin for 3 minutes to sterilise them, then packed into hand-rolled dung balls for incubation. These eggs successfully developed into adult beetles and were among those in the first batches released into the wild on 30 January  1968 in Lansdown, near Townsville, Queensland. This quarantine method was subsequently adopted at the CSIRO research base in Pretoria, South Africa, with the added step of the eggs being transported by air in sealed containers of sterile peat moss. First introductions. In the early years of the project, the dung beetle species "O. gazella", which was able to remove dung pads in as little as 24 hours, showed the most promise in becoming established in Australia. After the first stocks were released onto pastureland in Northern Queensland in 1968, beetle recapture figures showed that the species had spread at the  rate of 50–80 km per season, including the colonisation of two islands, Magnetic Island and Palm Island, 10 km and 30 km offshore respectively. By April 1970, "O. gazella" was firmly established over an area of 400 km. This performance however, was not uniformly excellent in all areas of Australia at all times of the season or year and so showed that Australia would need a number of other dung beetle species to fill the gaps in "O. gazella"'s activity. Australian Dung Beetle Project Research Unit in Pretoria. In order to find a suitable minifauna of beetles for Australia , Bornemissza set up the Australian Dung Beetle Research Unit in Pretoria, South Africa to find species that would complement the work of "O. gazella". South Africa was chosen as a location to study dung beetles for several reasons. Firstly, there are a large number of different species from which to choose from (some 800 species south of the Zambezi and Kunene rivers alone), and these species have co-evolved alongside large bovids (e.g. African buffalo) for many thousands of years. There are also homologies between the sub-tropical climates of areas of the two countries. The political climate in other  parts of Africa and the more advanced nature of scientific research in South Africa also made it an ideal location. The Plant Protection Research Institute in South Africa funded much of the work at this research base. Further field stations were also maintained in Cape Town during 1978-1980 and in Hluhluwe Game Reserve during 1981–1986. The aim was to find beetles that would match, as closely as possible, 8 selection criteria: While most of this research was carried out in South Africa, it was recognised that further study in other areas of the world would be useful in selecting  dung beetle species for introduction to Australian climates not matched by those in South Africa. To this end, a further research unit was set up in Montpellier, France, as a base from which to study European species that may be more suited for introduction to cooler, southern areas of Australia. Beetle collection surveys. Two types of field research were carried out in Pretoria to identify beetles that would be suitable for export to Australia. Firstly, researchers took stocktaking trips to locations around South Africa in order to collect data on the biodiversity and ecological abundance of the beetle fauna of  an area, as well as the environmental conditions preferred by different species. Secondly, species earmarked as being potential candidates for biological control were then collected and taken back to the research base laboratory where experiments were carried out to determine such things as beetle biology, habits, dung-disposing capacity, reproductive cycles and the ability to withstand quarantining procedures. It was necessary to devise and record methods of propagating dung beetles in large numbers, and this information forwarded onto the research base in Australia. In addition, extensive studies were carried out in the beetles' native habitat to study their likely distribution  patterns in Australia and thus aid in the selection of sites for their introduction. Later research has found that habitat specificity matching is one of the most important factors in determining whether or not an introduced species is successful in becoming established. Samples of beetles that had already been successfully introduced to Australia were also collected for further study. This was important so as to enrich the gene pool of beetle species within Australia and reduce the liability of any one species to become extinct because of a disease or chance mutation. Genetic variants were also imported to Hawaii in  order to enrich the genetic diversity in that region, and as a thank you to the island for being cooperative in the research. CSIRO Dung Beetle Research base in Canberra. On arrival at the Canberra research unit, beetle eggs were transferred to dung balls and incubated. Adult beetles were then bred in insectaries for two or more generations in sterile conditions to eliminate the possibility of co-breeding parasitic mites or diseases endemic to Africa such as foot-and-mouth. Some beetle species were rejected at this stage and not subsequently released onto pastureland because difficulty was experienced in rearing  those that entered a period of dormancy and some simply did not survive the stringent quarantining procedures. Beetles that successfully survived and reproduced were despatched by air or road to cooperating farmers or CSIRO staff. They were packed into ventilated crates of damp peat and thousands at a time were simply tipped onto fresh dung pads at the chosen release sites. Most beetles were observed to instantly bury themselves into the dung. From then on, farmers helped to monitor beetle distribution and activity and carried identification cards in their vehicles so that they could monitor beetle activity. The original review  paper by Bornemissza states that by 1975, 23 species of dung beetle, including 3 genetic variants, had been released. By 1984, this number was 43 species, although it was found that 20 of these failed to establish. As well as the original "star" of the program, "Onthophagus gazella", establishing well, other species such as "Euoniticellus intermedius", "Onthophagus binodis" and "Liatongus militaris" were shown to have reproduced in their thousands and became abundant enough to allow the transfer of subpopulations of certain colonies to establish in new areas. Further dung beetle research. The Australian Dung Beetle Project came to an end  in 1986 when the Australian Meat Research Committee (AMRC), which then became the Australian Meat and Livestock Research and Development Corporation, withdrew their funding due to a shift in the focus of their work from on-farm production to off-farm marketing. In 1998, interest in the project was revived when John Feehan, the manager of Soilcam, was invited by the Taroom Shire Landcare Group to give a seminar on the use of dung beetles. This led to Soilcam leading a two-week survey of the dung beetle fauna in south-eastern Queensland. The following December, groups of interested parties  including graziers, scientists, government agencies and community groups met in Brisbane to discuss the future direction of dung beetle activities in Australia, and this in turn led to the formation of the National Dung Beetle Steering Committee, chaired by Mick Alexander. This committee proposed that a dung beetle project be undertaken in Queensland, which became a reality, thanks to funding from the National Heritage Trust, in October 2000. The objective of the Queensland Dung Beetle Project was to provide the first comprehensive and quantitative survey of the distribution and abundance of dung beetles across Queensland since the original CSIRO project , and to redistribute successful introduced species to other areas where they might be beneficial. This project was entitled ""Improving Sustainable Land Management Systems in Queensland using Dung Beetles"" and lasted for two years from January 2001 until December 2002. The Queensland Dung Beetle project used pitfall traps at sites across Queensland to trap dung beetles, which were then identified and recorded. Out of the 29 species that were introduced to Queensland during the Australian Dung Beetle Project of 1965–1985, 15 of these were recaptured in 2001–2002. The most abundant and widely distributed of these were "Onthophagus gazella" and "Euoniticellus intermedius . Some species, including Copris diversus" and "Onitis westermanni", which had been released during the original CSIRO project, were not recovered at all in the Queensland Dung Beetle Project, suggesting that they have not become established. Others, for example "Onthophagus obliquus" and "Sisyphus infuscatus" have been recovered since their original release but were also not found during this study. The project identified three species, namely "Onitis caffer", "Copris elphenor", and "Onitis vanderkelleni" as candidates for redistribution to other areas of Queensland. These were chosen because, although they were not widely distributed across Queensland, they successfully established in the areas where they  were found and had desirably rapid dung-burying capabilities. The Queensland Dung Beetle Project concluded that one outcome of the study was to confirm the "outstanding success" of the original CSIRO project to select and introduce dung beetles into Australia, and ""the impact of this on soil, water and pasture health, and on control of pest flies is undoubtedly worth many millions of dollars a year"". Further to this, the success of the Australian Dung Beetle Project is claimed to be the reason why Australians can now enjoy a café culture, as up until the 1950s, bush flies were so  problematic that it was illegal for restaurants and cafés to offer outside dining unless a designated area was enclosed by fly-wire. A lesser effect has been had, however, on reducing the populations of Buffalo fly. This species of blood sucking fly remains a pest to livestock in Australia although they are present, but not pestilent in South Africa. Suggestions have been made that Australia needs further introduced species of predatory beetle (e.g. histerids) in order to help reduce the numbers of these flies. A report by Penny Edwards of Landcare Australia in 2007 revealed that 23 species of beetle  that had been introduced to Australia during the CSIRO project were still established species, whereas 20 had failed to establish (see table 1). The report recommended that in order to complete the work started by the Australian Dung Beetle Project, further action is required to: The Dung Beetle Ecosystem Engineers project commenced in 2017 and runs until 2022. The project has been tasked with the key objective of expanding the range of dung beetles in Australia and analysing their performance for livestock producers. Funding for the project has been contributed by the Australian Government Department of Agriculture and Water Resources  as part of its Rural R&D for Profit program, the MLA Donor Company, and various project partners.