Vibrio
Vibrio is a genus of Gram-negative bacteria, possessing a curved-rod (comma) shape, several species of which can cause foodborne infection, usually associated with eating undercooked seafood. Typically found in salt water, "Vibrio" species are facultative anaerobes that test positive for oxidase and do not form spores. All members of the genus are motile. They are able to have polar or lateral flagellum with or without sheaths. "Vibrio" species typically possess two chromosomes, which is unusual for bacteria. Each chromosome has a distinct and independent origin of replication, and are conserved together over time in the genus. Recent  phylogenies have been constructed based on a suite of genes (multilocus sequence analysis). O. F. Müller (1773, 1786) described eight species of the genus "Vibrio" (included in Infusoria), three of which were spirilliforms. Some of the other species are today assigned to eukaryote taxa, e.g., to the euglenoid "Peranema" or to the diatom "Bacillaria". However, "Vibrio" Müller, 1773 became regarded as the name of a zoological genus, and the name of the bacterial genus became "Vibrio" Pacini, 1854. Filippo Pacini isolated micro-organisms he called "vibrions" from cholera patients in 1854, because of their motility. In Latin "vibrio" means "to  quiver". "Vibrio" spp. are commonly found in marine environments. Marine "Vibrio" species are highly salt tolerant and can grow in wide range of salinity. S.I. Paul et al. (2021) isolated, characterized, and identified multiple strains of "Vibrio" species ("Vibrio alginolyticus, Vibrio natriegens, Vibrio pelagius, Vibrio azureus") from marine sponges of the Saint Martin's Island Area of the Bay of Bengal, Bangladesh. Where, "Vibrio" species were found most dominant bacteria in marine environment. Biochemical characteristics of "Vibrio" spp.. The genus "Vibrio" contains a large number of species. So, variation in the biochemical characteristics are most common in case of the  genus "Vibrio". Colony, morphological, physiological, and biochemical characteristics of the genus "Vibrio"  are shown in the Table below. Note: Group-1: "Vibrio alginolyticus"; Group-2: "Vibrio natriegens, Vibrio pelagius, Vibrio azureus"; + = Positive; – =Negative; V =Variable (+/–) Pathogenic strains. Several species of "Vibrio" are pathogens. Most disease-causing strains are associated with gastroenteritis, but can also infect open wounds and cause sepsis. They can be carried by numerous marine animals, such as crabs or prawns, and have been known to cause fatal infections in humans during exposure. Risk of clinical disease and death increases with certain factors, such as  uncontrolled diabetes, elevated iron levels (cirrhosis, sickle cell disease, hemochromatosis), and cancer or other immunocompromised states. Pathogenic "Vibrio" species include "V. cholerae" (the causative agent of cholera), "V. parahaemolyticus", and "V. vulnificus." "V. cholerae" is generally transmitted by contaminated water. Pathogenic "Vibrio" species can cause foodborne illness (infection), usually associated with eating undercooked seafood. When ingested "Vibrio" bacteria can primarily result in watery diarrhea along with other secondary symptoms.The pathogenic features can be linked to quorum sensing, where bacteria are able to express their virulence factor via their signalling molecules. "V. vulnificus" outbreaks commonly occur in warm climates and  small, generally lethal, outbreaks occur regularly. An outbreak occurred in New Orleans after Hurricane Katrina, and several lethal cases occur most years in Florida. As of 2013 in the United States, "Vibrio" infections as a whole were up 43% when compared with the rates observed in 2006–2008. "V. vulnificus", the most severe strain, has not increased. Foodborne "Vibrio" infections are most often associated with eating raw shellfish. "V. parahaemolyticus" is also associated with the Kanagawa phenomenon, in which strains isolated from human hosts (clinical isolates) are hemolytic on blood agar plates, while those isolated from nonhuman sources are not hemolytic . Many "Vibrio" species are also zoonotic. They cause disease in fish and shellfish, and are common causes of mortality among domestic marine life. Diagnosis. Cholera. "Cholera" A common sign of "Vibrio" infection is cholera. Cholera primarily presents with rapid water loss by watery diarrhea. Other symptoms include vomiting and muscle cramps. Water loss can lead to dehydration which can be mild to moderate to severe. Moderate to severe dehydration requires immediate treatment. "V. cholerae" is the most common pathogen that causes cholera. The gold standard for detecting cholera is through cultures of stool samples or rectal swabs. Identification is then  done through microscopy or by agglutination of antibodies. Cultures are done in thiosulfate citrate bile-salts sucrose agar. "V cholerae" will form yellow colonies. Vibriosis. Vibriosis is a sign of a more severe "Vibrio" infection. Common causes of vibriosis include consumption of raw or undercooked seafood, primarily oysters, or wound exposure to sea water. The majority of "V. parahemolyticus" infections can be self-limiting and symptoms include diarrhea, nausea, headaches, fever and chills. "V. vulnificus" can lead to a more serious disease, particularly in wound infection which can turn into necrotizing fasciitis. "V. parahaemolyticu"s is the most common pathogen in  vibriosis however, "V. vulnificus" is more common in people who have certain risk factors like older age, liver disease or diabetes mellitus. Like all vibrio diagnosis, vibriosis can also be determined in stool cultures. "V. parahemolyticus" and "V. vulnificus" will form green colonies. Treatment. Medical care depends on the clinical presentation and the presence of underlying medical conditions. Section::::"Vibrio" gastroenteritis. Because "Vibrio" gastroenteritis is self-limited in most patients, no specific medical therapy is required. Patients who cannot tolerate oral fluid replacement may require intravenous fluid therapy. Although most "Vibrio" species are sensitive to antibiotics such as doxycycline or  ciprofloxacin, antibiotic therapy does not shorten the course of the illness or the duration of pathogen excretion. However, if the patient is ill and has a high fever or an underlying medical condition, oral antibiotic therapy with doxycycline or ciprofloxacin can be initiated. Noncholera" Vibrio" infections. Patients with noncholera "Vibrio" wound infection or sepsis are much more ill and frequently have other medical conditions. Medical therapy consists of: Prevention. Cholera. The most effective method to prevent cholera is the improvement of water and food safety. This includes the sanitation of water, proper preparation of food and community awareness of outbreaks . Prevention has been most effective in countries where cholera is endemic. Another method is cholera vaccines. Examples of cholera vaccines include Dukoral and Vaxchora. Vibriosis. Prevention of vibriosis is mostly from food processing. Food items, mostly seafood, that commonly contain "vibrio" organisms are regularly controlled. Analysis of the water that seafood is fished or farmed from to determine microorganism content. Food processing methods like pasteurization and high pressure are used to eliminate microorganisms and pathogens. Other strains. "V. harveyi" is a pathogen of several aquatic animals, and is notable as a cause of luminous vibriosis in shrimp (prawns). "V. fischeri  (or Aliivibrio fishceri)" is known for its mutualistic symbiosis with the Hawaiian bobtail squid, which is dependent on microbial luminescence. Flagella. "Flagella" The "typical", early-discovered "Vibrio" species, such as "V. cholerae", have a single polar flagellum (monotrichous) with sheath. Some species, such as "V. parahaemolyticus" and "V. alginolyticus", have both a single polar flagellum with sheath and thin flagella projecting in all directions (peritrichous), and the other species, such as "V. fischeri", have tufts of polar flagella with sheath (lophotrichous). Structure. Typical bacterial flagellum structure contains three components: the basal body, the hook and the filament. Like typical bacteria , "Vibrio" spp, have these three components. In "Vibrio" spp, most have one flagella located on one pole of the bacterium. Another difference is the gradient used to power the flagella motor is sodium driven rather than proton driven. The flagellum is also surrounded by a sheath extending from the membrane. The purpose of this sheath has yet to be determined. Affect on Virulence. Motility is very important for "Vibrio" spp for infection. Research has shown that a variety of "Vibrios" mutants that are defective in flagella synthesis or non-motile are defective in infection. Loss of motility in "Vibrio" has  shown impaired colonization and adherence to host's intestines. Natural transformation. Natural transformation is a common bacterial adaptation for DNA transfer that employs numerous bacterial gene products. For a recipient bacterium to bind, take up, and recombine exogenous DNA into its chromosome, it must become competent, that is, enter a special physiologic state. The DNA-uptake process of naturally competent "V. cholerae" involves an extended competence-induced pilus and a DNA-binding protein that acts as a rachet and reels DNA into the periplasm. Natural transformation has also been described for "V. fisheri", "V. vulnificus" and "V. parahaemolyticus". Small RNA . "V. cholerae" has been used in discoveries of many bacterial small RNAs. Using sRNA-Seq and Northern blot candidate sRNAs were identified and characterised as IGR-sRNA (intragenic region), AS-sRNAs (transcribed from the antisense strand of the open reading frame (ORF) and ORF-derived. One of the candidates from this study, IGR7, was shown to be involved in carbon metabolism and later renamed MtlS RNA. Other sRNAs identified in "V. cholerae" through genetic screens and computational methods include Qrr RNA, Vibrio regulatory RNA of OmpA, MicX sRNA, Vibrio cholerae ToxT activated RNAs, tfoR RNA, and VqmR sRNA. Vibrio Vibrio