Wuchereria bancrofti
Wuchereria bancrofti is a filarial (arthropod-borne) nematode (roundworm) that is the major cause of lymphatic filariasis. It is one of the three parasitic worms, together with "Brugia malayi" and "B. timori", that infect the lymphatic system to cause lymphatic filariasis. These filarial worms are spread by a variety of mosquito vector species. "W. bancrofti" is the most prevalent of the three and affects over 120 million people, primarily in Central Africa and the Nile delta, South and Central America, the tropical regions of Asia including southern China, and the Pacific islands. If left untreated, the infection can  develop into a chronic disease called lymphatic filariasis. In rare conditions, it also causes tropical eosinophilia, an asthmatic disease. No vaccine is commercially available, but high rates of cure have been achieved with various antifilarial regimens and lymphatic filariasis is the target of the WHO Global Program to Eliminate Lymphatic Filariasis with the aim to eradicate the disease as a public-health problem by 2020. Signs and symptoms. The pathogenesis of "W. bancrofti" infection is dependent on the immune system and inflammatory responses of the host. After infection, the worms mature within 6–8 months, male and female worms mate and  then release the microfilariae. These microfilariae can be released for up to 10 years. Parasite. Morphology. As a dioecious worm, "W. bancrofti" exhibits sexual dimorphism. The adult worm is long, cylindrical, slender, and smooth with rounded ends. It is white in colour and almost transparent. The body is quite delicate, making removing it from tissues difficult. It has a short cephalic or head region connected to the main body by a short neck, which appears as a constriction. Dark spots are dispersed nuclei throughout the body cavity, with no nuclei at the tail tip. Males and females can be differentiated  by size and structure of their tail tips. The male worm is smaller, long and wide, and features a ventrally curved tail. The tip of the tail has 15 pairs of minute caudal papillae, the sensory organs. The anal region is an elaborate structure consisting of 12 pairs of papillae, of which eight are in front and four are behind the anus. In contrast, the female is to long and wide, nearly three times larger in diameter than the male. Its tail gradually tapers and rounded at the tip. No additional sensory structures are seen. Its vulva lies towards the  anterior region, about 0.25 mm from the head. Adult males and females are most often coiled together and are difficult to separate. Females are ovoviviparous and can produce thousands of juveniles known as microfilariae. The microfilaria is a miniature adult, and retains the egg membrane as a sheath, and is often considered an advanced embryo. It measures 280 μm long and 25 μm wide. It appears quite structureless "in vivo", but histological staining makes its primitive gut, nerve ring, and muscles apparent. Lifecycle. "W. bancrofti" carries out its lifecycle in two hosts. Humans serve as the definitive host and mosquitos  as the intermediate host. The adult parasites reside in the lymphatics of the human host. They are found mostly in the afferent lymphatic channels of the lymph glands in the lower part of the body. The first-stage larvae, known as microfilariae, are present in the circulation. The microfilariae have a membrane "sheath". This sheath, along with the area in which the worms reside, makes identification of the species of microfilariae in humans easier to determine. The microfilariae are found mainly in the peripheral blood and can be found at peak amounts from 10 pm to 4 am. They migrate  between the deep and the peripheral, circulation exhibiting unique diurnal periodicity. During the day, they are present in the deep veins, and during the night, they migrate to the peripheral circulation. The cause of this periodicity remains unknown, but the advantages of the microfilariae being in the peripheral blood during these hours may ensure the vector, the nighttime mosquito, will have a higher chance of transmitting them elsewhere. Physiological changes also are associated with sleeping, such as lowered body temperature, oxygen tension, and adrenal activity, and an increased carbon dioxide tension, among other physical alterations, any of which could be  the signals for the rhythmic behavior of microfilarial parasites. If the hosts sleep by day and are awake at night, their periodicity is reversed. In the South Pacific, where "W. bancrofti" shows diurnal periodicity, it is known as periodic. The microfilariae are transferred into a vector, which are most commonly mosquito species of the genera "Culex", "Anopheles", "Mansonia", and "Aedes". Inside the mosquito, the microfilariae mature into motile larvae called juveniles; these migrate to the labium after a period around 10 days. When the infected mosquito has its next blood meal, "W. bancrofti" larvae are deposited from the mouthparts onto  the skin of the prospective host and migrate through microcuts in the dermis or the tract created by the proboscis into the bloodstream of the new human host. The larvae move through the lymphatic system to regional lymph nodes, predominantly in the legs and genital area. The larvae develop into adult worms over the course of a year, and reach sexual maturity in the afferent lymphatic vessels. After mating, the adult female worm can produce thousands of microfilariae that migrate into the bloodstream. A mosquito vector can bite the infected human host, ingest the microfilariae, and thus repeat the lifecycle . The organism notably does not multiply within its intermediate host, the mosquito. Diagnosis. A blood smear is a simple and fairly accurate diagnostic tool, provided the blood sample is taken during the period in the day when the juveniles are in the peripheral circulation. Technicians analyzing the blood smear must be able to distinguish between "W. bancrofti" and other parasites potentially present. A polymerase chain reaction test can also be performed to detect a minute fraction, as little as 1 pg, of filarial DNA. Some infected people do not have microfilariae in their blood. As a result, tests aimed to  detect antigens from adult worms can be used. Ultrasonography can also be used to detect the movements and noises caused by the movement of adult worms. Dead, calcified worms can be detected by X-ray examinations. Prevention. Prevention focuses on protecting against mosquito bites in endemic regions. Insect repellents and mosquito nets are useful to protect against mosquito bites. Public education efforts must also be made within the endemic areas of the world to successfully lower the prevalence of "W. bancrofti" infections. Eradication. The WHO is coordinating an effort to eradicate filariasis. The mainstay of this programme is the mass  use of antifilarial drugs on a regular basis for at least five years. In April 2011, Sri Lanka was certified by the WHO as having eradicated this disease. Treatment. The severe symptoms caused by the parasite can be avoided by cleansing the skin, surgery, or the use of anthelmintic drugs, such as diethylcarbamazine, ivermectin, or albendazole. The drug of choice is diethylcarbamazine, which can eliminate the microfilariae from the blood and also kill the adult worms with a dose of 6 mg/kg/day for 12 days, semiannually or annually. A polytherapy treatment that includes ivermectin with diethylcarbamazine or albendazole  is more effective than either drug alone. Protection is similar to that of other mosquito-spread illnesses; one can use barriers both physical (a mosquito net), chemical (insect repellent), or mass chemotherapy as a method to control the spread of the disease. Mass chemotherapy should cover the entire endemic area at the same time. This will significantly decrease the overall microfilarial titer in blood in mass, hence decreasing the transmission through mosquitoes during their subsequent bites. Antibiotic active against the "Wolbachia" symbionts of the worm have been tested as treatment. "Wolbachia"-free worms immediately become sterile, and later die prematurely. Epidemiology . "W. bancrofti" is responsible for 90% of lymphatic filariasis. Recently, 120 million worldwide cases of lymphatic filariasis were estimated. "W. bancrofti" largely affects areas across the broad equatorial belt (Africa, the Nile Delta, Turkey, India, the East Indies, Southeast Asia, Philippines, Oceanic Islands, and parts of South America). The mosquito vectors of "W. bancrofti" have a preference for human blood; humans are apparently the only animals naturally infected with "W. bancrofti". No reservoir host is known, and the disease could, therefore, potentially be eradicated. History. The effects of "W. bancrofti" were documented early in ancient texts. Ancient Greek and Roman  writers noted the similarities between the enlarged limbs and thickened, cracked skin of infected individuals to that of elephants, hence the name elephantiasis to describe the disease. In 1862 in Paris, Jean-Nicolas Demarquay found what appeared to be nematode worms in the fluid aspirated from a hydrocele in a young man from Havana, Cuba. Unaware of this observation, three years later in Bahia, Brazil, Otto Wucherer found these same worms but this time in urine from a woman with chyluria. Ignorant of both these observations, Timothy Lewis in India in 1870 found them in the urine of an Indian  with chyluria then two years later found them in blood. Some of Lewis's specimens were examined in the same year in England by George Busk who named them "Filaria sanguinis hominis". In 1876 and 1877, Joseph Bancroft in Brisbane, Australia found adult worms in lymphatic abscesses in patients with larvae in the blood. He sent them to Spencer Cobbold in London who named them "Filaria Bancrofti". Patrick Manson in Xiamen, China (then called Amoy) made two important observations. Firstly he discovered in 1877 that if "Culex quinquefasciatus" and "Aedes aegypti" mosquitoes fed on a person with larvae (microfilariae) in  the blood, they moulted twice in the insects' abdomen and became larger worms now called infective larvae. Secondly, he found in 1879 that the blood-dwelling forms had a nocturnal periodicity with large numbers appearing in the blood around midnight with minimal numbers in the middle of the day. This coincided with the biting habits of these mosquitoes. Manson surmised that infected mosquitoes drowned and infective larvae were ingested in water. In 1899, Thomas Bancroft in Brisbane fed laboratory-reared mosquitoes on a patient with microfilaraemia, kept them for 16 days, then sent some specimens to George Low in London . Low prepared histological sections of the mosquitoes and found that the larvae migrated from the abdomen to the thorax to the salivary glands then passed down the proboscis suggesting that infective larvae were injected at a subsequent mosquito bite. In 1902, Thomas Bancroft proved that this was the mode of transmission using a related worm, "Dirofilaria immitis", and generated adult worms in experimentally infected dogs. In 1921, Léon Seurat erected the genus "Wuchereria" and placed this worm in it as "Wuchereria bancrofti". "W. bancrofti" is speculated to have been brought to the New World by the slave trade. Once it  was introduced to the New World, this filarial worm disease persisted throughout the areas surrounding Charleston, South Carolina, until its sudden disappearance in the 1920s.