Nitrogen balance
Nitrogen balance is a measure of nitrogen input minus nitrogen output. Nitrogen Balance = Nitrogen intake - Nitrogen loss Sources of nitrogen intake include meat, dairy, eggs, nuts and legumes, and grains and cereals. Examples of nitrogen losses include urine, feces, sweat, hair, and skin. Blood urea nitrogen can be used in estimating nitrogen balance, as can the urea concentration in urine. Nitrogen Balance and Protein Metabolism. Nitrogen is a fundamental component of amino acids, which are the molecular building blocks of protein. Therefore, measuring nitrogen inputs and losses can be used to study protein metabolism. Positive nitrogen balance  is associated with periods of growth, hypothyroidism, tissue repair, and pregnancy. This means that the intake of nitrogen into the body is greater than the loss of nitrogen from the body, so there is an increase in the total body pool of protein. Negative nitrogen balance is associated with burns, serious tissue injuries, fevers, hyperthyroidism, wasting diseases, and during periods of fasting. This means that the amount of nitrogen excreted from the body is greater than the amount of nitrogen ingested. A negative nitrogen balance can be used as part of a clinical evaluation of malnutrition. Nitrogen balance is the  traditional method of determining dietary protein requirements. Determining dietary protein requirements using nitrogen balance requires that all nitrogen inputs and losses are carefully collected, to ensure that all nitrogen exchange is accounted for. In order to control nitrogen inputs and losses, nitrogen balance studies usually require participants to eat very specific diets (so total nitrogen intake is known) and stay in the study location for the duration of the study (to collect all nitrogen losses). Because of these conditions, it can be difficult to study the dietary protein requirements of certain populations using the nitrogen balance technique (e.g. children). Dietary  nitrogen, from metabolising proteins and other nitrogen-containing compounds, has been linked to changes in genome evolution. Species which primarily obtain energy from metabolising nitrogen-rich compounds use more nitrogen in their DNA than species which primarily break down carbohydrates for their energy. Dietary nitrogen alters codon bias and genome composition in parasitic microorganisms.