Acrasin
Each species of slime mold has its own specific chemical messenger, which are collectively referred to as acrasins. These chemicals signal that many individual cells aggregate to form a single large cell or plasmodium. One of the earliest acrasins to be identified was cAMP, found in the species "Dictyostelium discoideum" by Brian Shaffer, which exhibits a complex swirling-pulsating spiral pattern when forming a pseudoplasmodium. The term acrasin was descriptively named after Acrasia from Edmund Spenser's Faerie Queene, who seduced men against their will and then transformed them into beasts. Acrasia is itself a play on the Greek  akrasia that describes loss of free will. Extraction. Brian Shaffer was the first to purify acrasin, now known to be cyclic AMP, in 1954, using methanol. Glorin, the acrasin of "P. violaceum", can be purified by inhibiting the acrasin-degrading enzyme acrasinase with alcohol, extracting with alcohol and separating with column chromatography.