Silent synapse
In neuroscience, a silent synapse is an excitatory glutamatergic synapse whose postsynaptic membrane contains NMDA-type glutamate receptors but no AMPA-type glutamate receptors. These synapses are named "silent" because normal AMPA receptor-mediated signaling is not present, rendering the synapse inactive under typical conditions. Silent synapses are typically considered to be immature glutamatergic synapses. As the brain matures, the relative number of silent synapses decreases. However, recent research on hippocampal silent synapses shows that while they may indeed be a developmental landmark in the formation of a synapse, that synapses can be "silenced" by activity, even once  they have acquired AMPA receptors. Thus, silence may be a state that synapses can visit many times during their lifetimes. Synaptic transmission. Normal transmission across a glutamatergic synapse relies on the neurotransmitter glutamate, the glutamate-specific AMPA receptor (AMPAR), and calcium ions. Calcium ion entry into the presynaptic terminal causes the presynaptic release of glutamate, which diffuses across the synaptic cleft, binding to glutamate receptors on the postsynaptic membrane. There are four subtypes of glutamate receptors: AMPA receptors (AMPARs) (formerly known as quisqualate receptors), NMDA receptors (NMDARs), kainate receptors, and metabotropic glutamate receptors (mGluRs). Most research has been focused on  the AMPARs and the NMDARs. When glutamate binds to AMPARs located on the postsynaptic membrane, they permit a mixed flow of Na and K to cross the cells membrane, causing a depolarization of the postsynaptic membrane. This localized depolarization is called an excitatory postsynaptic potential (EPSP). Silent synapses release glutamate as do prototypical glutamatergic synapses, but their postsynaptic membranes contain only NMDA—and possibly mGlu—receptors able to bind glutamate. Though AMPA receptors are not expressed in the postsynaptic membranes of silent synapses, they are stored in vesicles inside the postsynaptic cells, where they cannot detect extracellular glutamate, but  can be quickly inserted into the postsynaptic cell membrane in response to a tetanizing stimulus. The NMDAR is functionally similar to AMPAR except for two major differences: NMDARs carry ion currents composed of Na, K, but also (unlike most AMPAR) Ca; NMDARs also have a site inside their ion channel that binds magnesium ions (Mg). This magnesium binding site is located in the pore of the channel, at a place within the electrical field generated by the membrane potential. Normally, current will not flow through the NMDAR channel, even when it has bound glutamate. This is because the ion channel  associated with this receptor is plugged by magnesium, acting like a cork in a bottle. However, since the Mg is charged and is bound within the membrane's electric field, depolarization of the membrane potential above threshold can dislodge the magnesium, allowing current flow through the NMDAR channel. This gives the NMDAR the property of being voltage-dependent, in that it requires strong postsynaptic depolarization to allow ion flux. Characteristics. Silent synapses were proposed as an explanation for differences in quantal content of excitatory postsynaptic currents (EPSCs) mediated by AMPARs and NMDARs in hippocampal neurons. More direct evidence came from  experiments where only a few axons were stimulated. The stimulation of a silent synapse does not elicit EPSCs when the postsynaptic cell is clamped at -60 mV. Stimulation of a silent synapse "will" elicit EPSCs when the postsynaptic cell is depolarized beyond -40 mV. This is because they lack surface AMPAR to pass current at hyperpolarized potentials, but do possess NMDARs that will pass current at more positive potentials (because of relief of magnesium block). Moreover, the EPSCs elicited with depolarized membrane potentials can be completely blocked by D-APV, a selective NMDAR blocker. Activation. Silent synapses are activated via  the insertion of AMPARs into the postsynaptic membrane, a phenomenon commonly called "AMPA receptor trafficking." When glutamate binds to a strongly-depolarized postsynaptic cell (e.g., during Hebbian LTP), Ca quickly enters and binds to calmodulin. Calmodulin activates calcium/calmodulin-dependent protein kinase II (CaMKII), which — among other things — acts on AMPAR-containing vesicles near the postsynaptic membrane. CaMKII phosphorylates these AMPARs, which serves as a signal to insert them into the postsynaptic membrane. Once AMPARs are inserted, the synapse is no longer silent; activated synapses no longer require simultaneous pre- and postsynaptic activity in order to elicit EPSPs. After initial  activation (Early Long Term Potentiation), if the post synaptic neuron continues to be stimulated, it will adjust to become permanently excitable (Late Long Term Potentiation). It does this by changing its level of AMPA Receptor production which are then inserted into the membrane at the synapse. Evidence suggests that dendrite arborization and synapse maturation 1 (Dasm1), an Ig superfamily member, is involved in the maturation of synapses, essentially "awakening" the silent synapses. Competing Hypotheses. The characterization of silent synapses is an ongoing field of research and there are many things about them that are not yet known. Some of what  is currently accepted about the properties of silent synapses may still prove to be incorrect in whole or in part. Some controversies about silent synapses have however, been settled. For example, until recently, there were four competing hypotheses for the mechanisms of synapse silence: All four of these hypotheses had their adherents, but the first three were largely ruled out as a mechanism for synapse silence by work published before 2008. However, recent experiments have clearly established that silent synapses can be observed at brainstem synapses bearing postsynaptic AMPA receptors. This study favors the glutamate spillover hypothesis by showing that  at silent synapses the glutamate concentration is reduced. At least, this study indicates that the popular hypothesis of the postsynaptic silent synapses does not apply in all systems. Integration with other topics. The Role of Silent Synapses in Long Term Potentiation Neural Development AMPA Receptor Trafficking