Armatimonas rosea
Armatimonas rosea is a Gram-negative bacterium and also the first species to be characterized within the phylum Armatimonadota. The Armatimonadota were previously known as candidate phylum OP10. OP10 was composed solely of environmental 16S rRNA gene clone sequences prior to "A. rosea's" discovery. Discovery. "A. rosea" was originally isolated from the rhizoplane of the aquatic plant "Phragmites australis" inhabiting a freshwater lake in the Yamanashi prefecture in Japan. Cultivation and isolation were completed in a low nutrient medium, DTS. Relatives. The environmental 16S rRNA gene sequences belonging to the phylum "Armatimonadota" are currently sorted into six  groups. Groups 2, 5, and 6 consist solely of sequences. Group 1 contains "Armatimonas rosea", Group 3 contains "Chthonomonadetes calidirosea", and Group 4 contains "Fimbriimonas ginsengisoli". "Chthonomonas calidirosea" strain T49T, an aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, was isolated from geothermally heated soil at Hell's Gate, Tikitere, New Zealand. It is the first representative of a new class in the phylum "Armatimonadota". It represents the first cultured representative of the "Chthonomonadetes", corresponding with Group 3 of the phylum "Armatimonadota". "Fimbriimonas ginsengisoli", an aerobic, non-motile, mesophilic, rod-shaped bacterium, was isolated from a ginseng field  soil sample. It represents the first cultured representative of the "Fimbriimonadia" class, corresponding with Group 4 of the phylum "Armatimonadota". Characteristics. When grown on the relatively low-nutrient medium, R2A, colonies were pink pigmented, circular, smooth, significantly hard, relatively small (1–2 mm) in size after one week of incubation at 30 °C. "A.rosea" has an optimum temperature at 30–35 °C with a range of 20–40 °C. The optimum pH for growth was 6.5 with a range of 5.5–8.5. It is able to tolerate NaCl concentrations up to 0.5%. When examined by phase-contrast and transmission electron microscopy  cells were observed to be nonmotile, ovoid to rod shaped, with sizes ranging from 1.4 to 1.8 µm in width and 2.4–3.2 µm in length. Neither spores nor flagella were observed. When investigated in R2A media, cultures of "A. rosea" were observed to be aerobic and chemoheterotrophic with no evidence of growth under anaerobic conditions. It is negative for the following tests: nitrate respiration, fermentative growth, catalase, and cytochrome oxidase.