3T3 cells
3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. After landing a principal investigator position at the National Cancer Institute in Bethesda, Maryland, Tadaro repeated the isolation procedure from the NIH Swiss mouse embryo with his students and established NIH-3T3 cell line. Nomenclature. The '3T3' designation refers to the  abbreviation of "3-day transfer, inoculum cells." This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of cells per 20 cm dish (the second "3") continuously. The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3' cells. Since then, several cell lines have been established with this procotol: Characteristics. Swiss 3T3 can  be inhibited by temazepam and other benzodiazepines. These cells are also contact inhibited. The cells are sensitive to sarcoma virus and leukemia virus focus formation. 3T3 cells can be transformed with SV40 and some other polyomaviruses. Culture characteristics. Adherent cells grow as a monolayer. A confluent monolayer yields 40000 cells/cm. Expression. Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway. Cytogenetics. 3T3 mouse cells are hypertriploid. The modal chromosome number is 68, which occurs in 30% of cells. Higher ploidies occur at a much lower rate of  2.4%.