Tsou plot
The Tsou plot is a graphical method of determining the number and nature of the functional groups on an enzyme that are necessary for its catalytic activity. Theory of Tsou's method. Tsou Chen-Lu analysed the relationship between the functional groups of enzymes that are necessary for their biological activity and the loss of activity that occurs when the enzyme is treated with an irreversible inhibitor. Suppose now that there are formula_1 groups on each monomeric enzyme molecule that react equally fast with the modifying agent, and formula_2 of these are essential for catalytic activity. After modification  of an average of formula_3 groups on each molecule, the probability that any particular group has been modified is formula_4 and the probability that it remains unmodified is formula_5. For the enzyme molecule to retain activity, all of its formula_2 essential groups must remain unmodified, for which the probability is formula_7. The fraction formula_8 of activity remaining after modification of formula_3 groups per molecule must be and so This means that a plot of formula_12 against formula_3 should be a straight line. As the value of formula_2 is initially unknown one must draw plots with values 1, 2, 3, etc . to see which one gives a straight line. There are various complications with this analysis, as discussed in the original paper, and, more recently in a textbook. Experimental applications. Despite the possible objections, the Tsou plot gave clear results when applied by Paterson and Knowles to inactivation of pepsin by trimethyloxonium tetrafluoroborate (Meerwein's reagent), a reagent that modifies carboxyl residues in proteins. They were able to deduce from this experiment that three non-essential groups are modified without loss of activity, followed by two essential groups — two because assuming formula_15 yielded a straight line in the plot, whereas values  of 1 and 3 yielded curves, with a total of 13 residues modified, as illustrated in the figure. Tsou's plot has also given good results with other systems, such the type I dehydroquinase from "Salmonella typhi", for which modification of just one essential group by diethyl pyrocarbonate was sufficient to inactivate the enzyme. Alternative approach to the same question. A little before Tsou published his paper William Ray and Daniel Koshland had described a different way of investigating the number and nature of groups on an enzyme essential for activity. Their method depends on kinetic measurements, and cannot  be used, therefore, in cases where the modification is too fast for such measurements, such as the case of pepsin discussed above, but it complements Tsou's approach in useful ways. Suppose that an enzyme has two groups formula_16 and formula_17 that are both essential for the catalytic activity, so if either is lost the catalytic activity is also lost. If formula_16 is converted to an inactive form in a first-order reaction with rate constant formula_19, and formula_17 is inactivated in a first-order reaction with a different rate constant formula_21, then the remaining activity formula_22 after time formula_23  obeys an equation of the following form: in which formula_25 is the value of formula_22 when formula_27 and formula_28 is the observed first-order rate constant for inactivation, the sum of the rate constants for the separate reactions. The equation can be extended in an obvious way to the case more than two groups are essential. Ray and Koshland also described the properties to be observed when not all of the modified groups are essential.