ZBTB32
Zinc finger and BTB domain-containing protein 32 is a protein that in humans is encoded by the 1960 bp ZBTB32 gene. The 52 kDa protein (487 aa) is a transcriptional repressor and the gene is expressed in T and B cells upon activation, but also significantly in testis cells. It is a member of the Poxviruses and Zinc-finger (POZ) and Krüppel (POK) family of proteins, and was identified in multiple screens involving either immune cell tumorigenesis or immune cell development. The protein recruits histone modification enzymes to chromatin to affect gene activation. ZBTB32 recruits corepressors, such as  N-CoR and HDACs to its target genes, induces repressive chromatin states and acts cooperatively with other proteins, e.g. with Blimp-1, to suppress the transcription of genes . It contains a N-terminal BTB/POZ domain (IPR000210) or a SKP1/BTB/POZ domain (IPR011333), and three C-terminal zinc fingers, Znf_C2H2_sf. (IPR036236), Znf_C2H2_type domain (IPR013087), a Znf_RING/FYVE/PHD domain (IPR013083), followed by a putative UBZ4 domain. Nomenclature. Zinc finger and BTB domain-containing protein 32 is also known as: Interactions. Zbtb32 has been shown to interact with: Immune system. The expression of ZBTB32 is induced by inflammatory cytokines and promotes  proliferation of natural killer cells. Zbtb32 knockout mice show a trend to develop type 1 diabetes, although the difference is not statistically different. Furthermore the Zbtb32 do not show a difference in lymphocyte proliferation, possibly due to compensation from other genes. Cancer. ZBTB32 is highly expressed spermatogonial stem cells, in hematopoietic stem and progenitor cells, in diffuse large B-cell lymphoma (DLBCL) and appears to suppress the immune system by silencing the CIITA gene. The transcription factor gene "GATA3" is altered in mammary tumors. Down-regulation of "GATA3" expression and activity by the Zinc-finger elbow-related proline domain protein  2 (Zpo2), whereas Zbtb32 facilitates Zpo2 targeting to the "GATA3" promoter, results in the development of aggressive breast cancers. A DNA methylation correlation network was built based on the methylation correlation between differentially methylated genes. A survival analysis of candidate biomarkers was performed. One of eight biomarkers and hub genes identified in colon cancer is "ZBTB32". The expression of Zbtb32 is upregulated after exposure to cisplatin.