Japanese Bamboo Shakuhachi Flute in two parts. Age related wear Length 21 1/2 (54.5 cm.). For malondialdehyde (MDA) level, the kidney tissues were homogenized in 0.15 M potassium chloride solution and supernatant had been deproteinized. Then, the samples have been treated with thiobarbituric acid for color formation. Absorbances were measured at 535 nm by spectrophotometry and the results were calculated as nmol/g tissue . For superoxide dismutase (SOD) activity, the renal tissues were homogenized in physiological saline; supernatant was measured after the centrifugation. Results were calculated as unit/g tissue, assuming 50% inhibition of activity as one unit enzyme activity . For determination of Nitric oxide (NO) levels, renal tissues were homogenized in a buffer; then deproteinized by centrifugation and NO was studied from supernatant. Results were calculated as mol/g of tissue . “Uthe uzobe elokhu ehlala eqhamuka ngapha eNorth West ezozilolonga uma esibona isidingo. “Angicabangi ukuthi ukuyozinza ePretoria kuzomphazamisa ezingeni abesekulona. “Ungumuntu osekhulile kulomdlalo noseyiqonda kahle into ayifunayo ngempilo yakhe,” kubeka uVerster.