Effects of LMVsmiRCtrl and LMVsmiR34a on the levels of | Open-i
fig5: Effects of LMVsmiRCtrl and LMVsmiR34a on the levels of caspase-3 and Tim-3 protein in AML cells. (a) The activity of caspase-3 in AML cells which was determined by ELISA. (b) The expression of Tim-3 protein in AML cells which was determined by western blot. Data represents mean ± SEM. ∗P < 0.05 versus veh; #P < 0.05 versus LMVsmiRCtrl. Veh: fresh culture medium; LMVsmiRCtrl: LMVs generated from miRCtrl transfected LSCs; and LMVsmiR34a: LMVs generated from miR34a mimic transfected LSCs.
Mentions: The activity of caspase-3 was reduced in AML cells coincubated with LMVsmiRCtrl (P < 0.05 versus veh; Figure 5(a)). LMVsmiR34a significantly increased the activity of caspase-3 in AML cells (P < 0.05 versus veh; Figure 5(a)). The expression of Tim-3 was upregulated in LMVsmiRCtrl-treated AML cells (P < 0.05 versus veh; Figure 5(b)) but was downregulated in LMVsmiR34a-treated AML cells (P < 0.05 versus veh; Figure 5(b)).