1.5 hours confirm positives Decreases enrichment time Requires confirmation Provides screening Semi-automated (plate reader) ELISA Based Tests Enzyme Immunoassay ( see examples ) TECRA VIA &quot; sandwich &quot; ELISA very rapid results (~ 4 hours) for certain tests (i.e., Bacillus Diarrheal Enterotoxin) manual TECRA Unique Antibody coated &quot;dipstick&quot; Convenient kit for washings and conjugate Sampling to results in &lt;22 hours Used for screening Positives must be confirmed Manual Mini VIDAS (bioMerieux) Fully automated ELISA machine Uses test strips containing: sample wash conjugate substrate Rapid results &lt;2 hours for certain toxins &lt;24 hours for E. coli O157 &lt;48 hours for Listeria Easy to use Expensive Transia Elisamatic II Fully automated ELISA machine Polymerase Chain Reaction (PCR) Based Tests [ see example ] BAX (Qualicon) Method sample enrichment - 20 hours cells lysed PCR performed blot DNA fragments probe for specific sequence interpret results Very sensitive Results in about 30 hours Used for screening Requires technician proficiency Requires specialized equipment Semi-automated Ribotyping RiboPrinter (Qualicon) DNA fingerprinting Detects rRNA (&quot;housekeeping&quot;) genes - highly conserved Method uses isolated colony DNA extracted and digested fragments separated by gel electrophoresis fragments blotted and hybridized with probe unique chemiluminescent pattern results compared to database Can differentiate to the sub-species level Useful for determining sources of contamination , bacterial relatedness Easy to use Expensive Fully automated Ice Nucleation Assay Bind (IDEXX) Uses bacteriophage to induce development of ice nucleation proteins Method sample enrichment 18-22 hours dilution and pipetting into assay tube bacteriophage infects cell ice nucleation proteins formed samples cooled to a specific temperature positives freeze and cause color change Rapid Requires specialized equipment Recommended Reading Food Microbiology: Fundamentals and Frontiers (Doyle et al.
(Rapid; Requires; specialized equipment Recommended Reading Food Microbiology:) (Method; uses; isolated colony DNA extracted and digested fragments) (Listeria Easy; to use; Expensive Transia Elisamatic II) (BAX (Qualicon) Method sample enrichment; Requires; specialized equipment Semi-automated) Context(BAX ( Qualicon ) Method sample enrichment Requires,List([845, 1087))):(Ribotyping RiboPrinter; Can differentiate; to the sub-species level Useful for determining sources of contamination , bacterial relatedness Easy to use Expensive Fully automated Ice Nucleation Assay Bind (IDEXX) Uses bacteriophage to induce development of ice nucleation proteins Method sample enrichment 18-22 hours dilution and pipetting into) (manual TECRA Unique Antibody; coated; ) (specialized equipment; Semi-automated; ) (Manual Mini VIDAS (bioMerieux) Fully automated ELISA machine Uses test strips; containing; ) (assay tube bacteriophage infects cell ice nucleation proteins formed samples; cooled; to a specific temperature positives) (confirmation Provides; screening; ELISA Based Tests Enzyme Immunoassay ( see examples) (Uses bacteriophage; to induce; development of ice nucleation proteins) (PCR; performed Used; blot DNA fragments probe) (Recommended; Reading; Food Microbiology) (Uses test; [is] Fully automated machine [of]; ELISA) (Polymerase Chain Reaction; [is] Fully automated machine [of]; ELISA)
BAX ( Qualicon ) Method sample enrichment Requires,List([845, 1087))
Rapid Method Listeria Easy BAX (Qualicon) Method sample enrichment Ribotyping RiboPrinter manual TECRA Unique Antibody specialized equipment Manual Mini VIDAS (bioMerieux) Fully automated ELISA machine Uses test strips assay tube bacteriophage infects cell ice nucleation proteins formed samples confirmation Provides Uses bacteriophage PCR Recommended Uses test Polymerase Chain Reaction
Requires uses to use Requires Can differentiate coated Semi-automated containing cooled screening to induce performed Used Reading [is] Fully automated machine [of] [is] Fully automated machine [of]
specialized equipment Recommended Reading Food Microbiology:
isolated colony DNA extracted and digested fragments
Expensive Transia Elisamatic II
specialized equipment Semi-automated
to the sub-species level Useful for determining sources of contamination , bacterial relatedness Easy to use Expensive Fully automated Ice Nucleation Assay Bind (IDEXX) Uses bacteriophage to induce development of ice nucleation proteins Method sample enrichment 18-22 hours dilution and pipetting into
to a specific temperature positives
ELISA Based Tests Enzyme Immunoassay ( see examples
development of ice nucleation proteins
blot DNA fragments probe
Food Microbiology
ELISA
ELISA