The method comprises the steps of obtaining pollen from a first plant, applying a lawn of Agrobacteria to a solid pollen culture medium, the Agrobacterium comprising at least one heterologous gene sequence capable of being transferred to a plant cell, applying the pollen to the solid medium, allowing the pollen to germinate and grow on the medium, whereby the Agrobacterium attaches to the pollen tube or mediates transfer of the heterologous gene sequence to the germinating pollen to obtain transgenic pollen, applying the transgenic pollen to the stigma of a second plant capable of being fertilized by the pollen of the first plant, thereby fertilizing the second plant, obtaining transgenic seed from the second plant and germinating the transgenic seed to obtain a transgenic plant.
(The method; comprises; the steps of obtaining pollen from a first plant) (the Agrobacterium; attaches; ) (the Agrobacterium; comprising; at least one heterologous gene sequence capable of being transferred to a plant cell) (the pollen; to grow; L:on the medium)
The method the Agrobacterium the Agrobacterium the pollen
comprises attaches comprising to grow
the steps of obtaining pollen from a first plant
at least one heterologous gene sequence capable of being transferred to a plant cell
L:on the medium