Materials The following items should be provided for each four students: Four sterile 250 ml Erlenmeyer flasks Four balloons to fit over the tops of the flasks One yeast cake One plastic graduated cylinder (100 mL capacity) One wooden applicator stick Four pastuer pipettes with droppers One wax marking pencil Four glass slides and cover slips One dropper bottle of 1% methylene blue stain The following items should be provided for class use: Two hundred and fifty ml of 10% solutions of the following sugars:a) glucose, b) lactose, c) sucrose, d) maltose Microscopes Sterile distilled water to rinse the graduated cylinder Tape measure Procedure The following procedure should be performed by students in a group of four: Using the graduated cylinder, fill each of the four flasks with a different sugar solution.
(One wax; marking; pencil) (The following items; should be provided; for each four students) (maltose Microscopes Sterile; distilled; water to rinse) (Procedure The following procedure; should be performed; by students in a group of four) (Four sterile 250 ml Erlenmeyer; flasks; Four balloons to fit over the tops of the flasks) Context(Four sterile 250 ml Erlenmeyer flasks,List([73, 110))):(blue stain The following items; should be provided; for class use) (Four balloons; to fit; L:over the tops of the flasks)
Four sterile 250 ml Erlenmeyer flasks,List([73, 110))
One wax The following items maltose Microscopes Sterile Procedure The following procedure Four sterile 250 ml Erlenmeyer blue stain The following items Four balloons
marking should be provided distilled should be performed flasks should be provided to fit
pencil
for each four students
water to rinse
by students in a group of four
Four balloons to fit over the tops of the flasks
for class use
L:over the tops of the flasks