Assertion: sperm | /r/HasProperty | incubated with secondary antibody
Facets:
value: after fixing
type: TEMPORAL
value: after washing
type: TEMPORAL
Source sentences:
text: Sperm were capacitated for 3 hr, and then incubated in 280 μg/ml scrambled IgY (scIgY) or scrambled rabbit IgG (scIgG), or with normal rabbit serum (NRS) or anti-calreticulin antibodies; after washing, sperm were fixed and incubated with a secondary antibody.
text: After washing and fixing, sperm were incubated with a secondary antibody (top row).