Assertion: zygote's nucleus | /r/HasProperty | treated with 0.5 mg/ml proteinase k
Facets:
value: overnight
type: TEMPORAL
Source sentences:
text: After heat inactivation at 65 °C for 20 min, the nuclei were treated with 0.5 mg/ml proteinase K in an equal volume of 2× digestion buffer (20 mM Tris·HCl (pH 7.4), 200 mM NaCl, 2% SDS and 10 mM EDTA) at 55 °C overnight.