Assertion: spheroid's cell | /r/HasProperty | incubated with pe mouse anti-human cd44
Facets:
value: in blocking solution
type: TEMPORAL
value: next
type: DEGREE
Source sentences:
text: Next, the cells were incubated with PE Mouse Anti-Human CD44 (BD Pharmingen, CA, USA) or PE Mouse Anti-Human CD54 (BD Pharmingen) in blocking solution for 1 h according to the supplier's instructions, after which the cells were washed three times with PBS-T. Nuclei were stained with Hoechst 33342