Assertion: spheroid's cell | /r/HasProperty | transfected with sirna
Facets:
value: against mct4
type: LOCATION
value: against nbcn1
type: LOCATION
value: transiently
type: MANNER
value: subsequently
type: TEMPORAL
Source sentences:
text: NHE1 was knocked out by CRISPR/Cas9 in MDA-MB-231 cells 12 and the cells were subsequently transiently transfected with siRNA against MCT4 or NBCn1, and grown as spheroids for 9 days before being lysed and subjected to western blotting using an antibody recognizing total and cleaved (c)PARP.