Assertion: protein roadblock | /r/CapableOf | inhibit muth activation
Facets:
value: by 70–80%
type: OTHER
value: substantially
type: DEGREE
Source sentences:
text: We show that a protein roadblock (EcoRI E111Q , a hydrolytically defective form of EcoRI endonuclease) placed on the helix between the two DNA sites inhibits MutH activation by 70–80% and that events that escape inhibition are attributable, at least in part, to diffusion of EcoRI E111Q away from its recognition site.
text: Our results show that a helix-bound protein roadblock substantially inhibits MutH activation.
text: The results presented here demonstrate that an EcoRI E111Q protein roadblock placed between the mismatch and the d(GATC)-strand signal inhibits MutH activation by 70–80%, results that are most readily interpreted in terms of a mechanism in which MutH activation depends on signaling along the DNA helix.