Assertion: osteocyte | /r/HasProperty | cultured with m-csf+tnf-α
Facets:
value: for 1 day
type: TEMPORAL
Source sentences:
text: To test the significance of cell-to-cell contact, an osteocyte-conditioned medium was prepared as follows: osteocytes were cultured with M-CSF+TNF-α (100 ng/ml) for 1 day, the medium was then aspirated and added to TNFR I, II deficient osteoclast precursor, as a positive control TNFR I, II deficient osteoclast precursor were cultured with M-CSF+RANKL (100 ng/ml).