Assertion: polypeptide's acid sequence | /r/AtLocation | seq id
Facets:
Source sentences:
text: Thus, in a first aspect the present invention relates to an IFNG polypeptide variant exhibiting IFNG activity and having the amino acid sequence shown in SEQ ID NO:12 ([S99T]huIFNG-132).
text: If this codon is completed appropriately the sequence codes for either of two N-terminal amino acid sequences shown as SEQ ID
text: In a highly preferred embodiment of the invention, the variant comprises at least one further modification, such as 1-10 further modifications, relative to the amino acid sequence shown in SEQ ID NO:12.
text: Another aspect of the present invention relates to a nucleotide sequence encoding an IFNG polypeptide variant exhibiting IFNG activity and having the amino acid sequence shown in SEQ ID NO:12.
text: In a still further aspect the present invention relates to a nucleotide sequence encoding an IFNG polypeptide variant exhibiting IFNG activity, wherein said variant comprises at least one modification, such as 1-10 modifications, relative to the amino acid sequence shown in SEQ ID NO:12.
text: An additional aspect of the present invention relates to a nucleotide sequence encoding a polypeptide having the amino acid sequence shown in SEQ ID NO:29.
text: In a still further aspect the present invention relates a population of IFNG polypeptide variants, or to a composition comprising a population of IFNG polypeptide variants, wherein said population comprises at least 70% of the IFNG polypeptide variant having the amino acid sequence shown in SEQ ID NO:12.
text: In a highly preferred embodiment of the invention, said population comprises at least 70% of an IFNG polypeptide variant which comprises at least one further modification, such as 1-10 further modifications, relative to the amino acid sequence shown in SEQ ID NO:12.
text: The terminology used for identifying amino acid positions/substitutions is illustrated as follows: G18 indicates position 18 occupied by glycine in the amino acid sequence shown in SEQ ID NO:1 or SEQ ID
text: The term “rhuIFNG” is intended to cover the mature form of wild-type human IFNG having the amino acid sequence shown in SEQ ID NO:17, which has been produced by recombinant means.
text: Although the parent polypeptide to be modified by the present invention may be any polypeptide with IFNG activity, and thus be derived from any origin, e.g. a non-human mammalian origin, it is preferred that the parent polypeptide is huIFNG with the amino acid sequence shown in SEQ ID NO:17 or a fragment thereof,
text: Accordingly, in a first aspect the present invention relates to an IFNG polypeptide variant exhibiting IFNG activity and having the amino acid sequence shown in SEQ ID NO:1 (i.e. [S99T]huIFNG), or fragment thereof, such as SEQ ID
text: E119N+S121T, P122N+A124S/T, A123N+K125S/T, A124N, A124N+T126S, K125N+G127S/T, T126N+K128S/T, G127N+R129S/T, K128N+K130S/T, R129N+R131S/T, K130N, K130N+S132T, R131N+Q133S/T, S132N+M134S/T, Q133N+L135S/T, M134N+F136S/T, L135N+R137S/T, F136N+G138S/T, R137N+R139S/T, G138N+R140S/T, R139N+A141S/T, R140N and R140N+S142T, the substitution being indicated relative to [S99T]huIFNG with the amino acid sequence shown in SEQ ID
text: A124N, A124N+T126S, K125N+G127S/T, T126N+K128S/T, G127N+R129S/T, K128N+K130S/T, R129N+R131S/T, K130N, K130N+S132T, R131N+Q133S/T, S132N+M134S/T, Q133N+L135S/T, M134N+F136S/T, L135N+R137S/T, F136N+G138S/T, R137N+R139S/T, G138N+R140S/T, R139N+A141S/T, R140N and R140N+S142T, the substitution being indicated relative to [S99T]huIFNG with the amino acid sequence shown in SEQ ID
text: For instance, substitutions that lead to introduction of a cysteine residue at positions exposed at the surface of the IFNG polypeptide and occupied by amino acid residue having at least 25% of the side chain exposed to the surface (in a structure with receptor molecule) include: Q1C, D2C, P3C, K6C, E9C, N10C, K13C, Y14C, N16C, G18C, H19C, D21C, N25C, G26C, G30C, K34C, N35C, K37C, E38C, E39C, S40C, K55C, K58C, N59C, K61C, D62C, D63C, Q64C, S65C, Q67C, K68C, E71C, T72C, K74C, E75C, N78C, V79C, K80C, N83C, S84C, N85C, K86C, K87C, D90C, E93C, K94C, T101C, D102C, L103C, N104C and E119C, the substitution being indicated relative to [S99T]huIFNG with the amino acid sequence shown in SEQ ID
text: Substitutions that lead to introduction of a cysteine residue at positions exposed at the surface of the IFNG polypeptide and occupied by amino acid residue having at least 50% of the side chain exposed to the surface (in a structure with receptor molecule) include: P3C, K6C, N10C, K13C, N16C, D21C, N25C, G26C, G31C, K34C, K37C, E38C, E39C, K55C, K58C, N59C, D62C, Q64C, S65C, K68C, E71C, E75C, N83C, S84C, K86C, K87C, K94C, T101C, D102C, L103C and N104C, the substitution being indicated relative to [S99T]huIFNG with the amino acid sequence shown in SEQ ID
text: Accordingly, in a very interesting aspect the present invention relates to an IFNG polypeptide variant exhibiting IFNG activity and having the amino acid sequence shown in SEQ ID NO:12 ([S99T]huIFNG-132).
text: In a highly preferred embodiment of the invention, the variant comprises at least one further modification, such as 1-10 further modifications, relative to the amino acid sequence shown in SEQ ID NO:12.
text: Another aspect of the present invention relates to a nucleotide sequence encoding an IFNG polypeptide variant exhibiting IFNG activity and having the amino acid sequence shown in SEQ ID NO:12.
text: In a still further aspect the present invention relates to a nucleotide sequence encoding an IFNG polypeptide variant exhibiting IFNG activity, wherein said variant comprises at least one modification, such as 1-10 modifications, relative to the amino acid sequence shown in SEQ ID NO:12.
text: An additional aspect of the present invention relates to a nucleotide sequence encoding a polypeptide having the amino acid sequence shown in SEQ ID NO:29.