Assertion: polypeptide's activity | /r/HasProperty | second antigen-binding
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Source sentences:
text: And a method for controlling association between polypeptides by modifying an amino acid constituting an interface formed when a polypeptide having a first antigen-binding activity and a polypeptide having a second antigen-binding activity are associated with each other.
text: The present invention provides a method for producing a polypeptide multimer comprising a polypeptide having a first antigen binding activity and a polypeptide having a second antigen binding activity or no antigen binding activity.
text: Having a first antigen-binding activity such that the polypeptide having the first antigen-binding activity and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity have a different binding force to protein A One or a plurality of amino acid residues are modified in both or any one of the polypeptide and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity.
text: Having a first antigen-binding activity such that the polypeptide having the first antigen-binding activity and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity have a different binding force to protein A One or a plurality of amino acid residues are modified in both or any one of the polypeptide and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity.
text: Accordingly, the present invention relates to a polypeptide having a first antigen-binding activity, a polypeptide having a second antigen-binding activity or not having an antigen-binding activity, and a polypeptide having one or two third antigen-binding activities.
text: Having a first antigen-binding activity such that the polypeptide having the first antigen-binding activity and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity have a different binding force to protein
text: A Provided is a method in which one or more amino acid residues are modified in both or any one of a polypeptide and a polypeptide having a second antigen-binding activity or not having an antigen-binding activity.
text: The present invention relates to a polypeptide having a first antigen-binding activity, a polypeptide having a second antigen-binding activity or not having an antigen-binding activity, and a polypeptide having one or two third antigen-binding activities.
text: Having a first antigen-binding activity such that the polypeptide having the first antigen-binding activity and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity have a different binding force to protein
text: A Provided is a method in which one or more amino acid residues are modified in both or any one of a polypeptide and a polypeptide having a second antigen-binding activity or not having an antigen-binding activity.
text: The polypeptide having the first antigen-binding activity and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity of the present invention has the amino acid sequence of the antibody heavy chain constant region or the amino acid sequence of the antibody Fc region.
text: Specifically, for example, when modifying the heavy chain constant region, EU numbering positions 356 and 439, 357 and 370 in the amino acid sequence of the heavy chain constant region of the polypeptide having the first antigen-binding activity, and EU of the heavy chain constant region of a polypeptide having the second antigen-binding activity or not having the antigen-binding activity, by modifying at least one of the combination of positions 399 and 409 to an amino acid having the same charge
text: The present invention also provides a method for purifying a polypeptide multimer comprising a polypeptide having a first antigen binding activity and a polypeptide having a second antigen binding activity or no antigen binding activity.
text: Accordingly, the present invention includes a polypeptide having a first antigen binding activity, a polypeptide having a second antigen binding activity, a polypeptide having a third antigen binding activity, and a polypeptide having a fourth antigen binding activity.
text: The present invention also provides a polypeptide having a first antigen binding activity, a polypeptide having a second antigen binding activity, a polypeptide having a third antigen binding activity, and a polypeptide having a fourth antigen binding activity.
text: A polypeptide comprising an amino acid sequence of a weight chain, a polypeptide having a second antigen binding activity comprising an amino acid sequence of an antibody light chain variable region and an amino acid sequence of an antibody heavy chain constant region, a third antigen binding A polypeptide comprising an amino acid sequence of an antibody light chain, wherein the polypeptide having an activity comprises a polypeptide comprising an amino acid sequence of an antibody heavy chain variable region and an amino acid sequence of an antibody light chain constant region; It can also be used.
text: Using the difference in the binding strength of the polypeptide having the first antigen-binding activity and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity to protein A, using protein A affinity chromatography, It is possible to produce or purify a polypeptide multimer comprising a polypeptide having a first antigen binding activity and a polypeptide having a second antigen binding activity or no antigen binding activity.
text: ・ Modification of amino acids to increase the hetero-association rate of two types of heavy chains of bispecific antibody. ・ Polypeptide having first antigen binding activity and second antigen binding activity or no antigen binding activity.
text: In addition, the polypeptide having the first antigen binding activity has the Kabat numbering 39th in the heavy chain variable region and / or the EU numbering 213 in the heavy chain constant region, and has the second antigen binding activity or has the antigen binding activity.