Assertion: osteoblast | /r/CapableOf | express gfp
Facets:
value: by activated cell sorting
type: MANNER
value: upon plating
type: TEMPORAL
Source sentences:
text: Isolation of osteoblasts expressing GFP by fluorescence-activated cell sorting from four calvarial bones of newborn mice carrying Osx1-GFP::Cre and Oct4-EGFP was performed as described in Liu et al. .
text: Upon plating, more than 90% osteoblasts showed GFP expression (Figure 1(b)).
text: We first isolated osteoblasts expressing GFP by fluorescence-activated cell sorting (FACS) from 4 calvarial bone of newborn mice harboring both transgenes, whereas no GFP expression was observed in calvarial bone derived from Oct4-EGFP transgenic mice.