Assertion: polypeptide | /r/HasAspect | antigen-binding activity
Facets:
Source sentences:
text: By combining a polypeptide having no antigen-binding activity and a polypeptide having an antigen-binding activity, it is also possible to produce a polypeptide multimer that binds monovalently to an antigen.
text: The polypeptide having antigen binding activity may be, for example, a molecule having a structure in which at least two of a heavy chain variable region, a light chain variable region, a heavy chain constant region, and a light chain constant region are linked as a single chain. .
text: The modification site is not limited as long as it is an amino acid that forms an interface in a polypeptide having two antigen-binding activities.
text: In the present invention, “the difference in the binding ability of a polypeptide having an antigen-binding activity to
text: A polypeptide having antigen-binding activity in which one or more amino acid residues are modified is prepared by preparing a DNA encoding a polypeptide having antigen-binding activity or not having antigen-binding activity, and one or more bases of the DNA Can be obtained by introducing the product into cells well known to those skilled in the art, culturing the cells to express these DNAs, and recovering the expression products.
text: That is, the polypeptide having antigen-binding activity can contain the amino acid sequence of an antibody variable region, a receptor, a fusion peptide of a receptor and an Fc region, Scaffold, or a fragment thereof.
text: A polypeptide having antigen-binding activity can also be expressed as a polypeptide having an antigen-binding protein domain.
text: Therefore, “the difference in the binding ability of a polypeptide having antigen-binding activity to
text: protein A” means “when two or more polypeptides having antigen-binding activity are eluted using protein A affinity chromatography.
text: In the present invention, it is preferable to modify an amino acid on the surface of a polypeptide having antigen-binding activity or not having antigen-binding activity.
text: In addition, the polypeptide having antigen-binding activity can further contain additional modifications in addition to the above-described amino acid sequence modifications.
text: The amino acid to be modified is preferably an amino acid exposed on the surface of a polypeptide having antigen-binding activity.
text: That is, the polypeptide having no antigen-binding activity can include, but is not limited to, the antibody constant region, Fc region, Scaffold, or the amino acid sequence of these fragments.
text: Further, in the present invention, the “polypeptide having the first antigen-binding activity” can be expressed as “the first polypeptide having the antigen-binding activity”.
text: The present invention provides a method for producing a polypeptide multimer comprising a polypeptide having a first antigen binding activity and a polypeptide having a second antigen binding activity or no antigen binding activity.
text: The present invention also provides a method for purifying a polypeptide multimer comprising a polypeptide having a first antigen binding activity and a polypeptide having a second antigen binding activity or no antigen binding activity.
text: Using the difference in the binding strength of the polypeptide having the first antigen-binding activity and the polypeptide having the second antigen-binding activity or not having the antigen-binding activity to protein A, using protein A affinity chromatography, It is possible to produce or purify a polypeptide multimer comprising a polypeptide having a first antigen binding activity and a polypeptide having a second antigen binding activity or no antigen binding activity.
text: ・ Modification of amino acids to increase the hetero-association rate of two types of heavy chains of bispecific antibody. ・ Polypeptide having first antigen binding activity and second antigen binding activity or no antigen binding activity.
text: The polypeptide having the antigen binding activity may be either a biological one or an artificially designed one.
text: In addition, the polypeptide having the first antigen binding activity has the Kabat numbering 39th in the heavy chain variable region and / or the EU numbering 213 in the heavy chain constant region, and has the second antigen binding activity or has the antigen binding activity.