Assertion: osteoblast | /r/HasProperty | fixed in 1× pbs
Facets:
value: for 15 mins
type: TEMPORAL
Source sentences:
text: Osteoblasts were fixed in 10% formaldehyde in 1× PBS for 15 mins, stained with 40 mM Alizarin Red S (Sigma) solution for 30 seconds to 5 minutes, and washed 3 times with distilled water to remove excess staining solution.