Assertion: peptide's residue | /r/HasProperty | mutated to alanine
Facets:
value: to prevent splicing
type: PURPOSE
Source sentences:
text: GFP fluorescence was not detected when one of the three constructs was absent or when +1 extein residues of each split intein is mutated to alanine (C65A for intein A and S86A for intein B) to prevent splicing.