Assertion: lipid's acid | /r/HasProperty | followed by heat treatment
Facets:
Source sentences:
text: Subsequently, these samples are prepared by following SPV reaction methods: (1) suspend tested samples in water and place in a glass tube; (2) add concentrated sulfuric acid followed by heat treatment and ice bath; (3) add freshly prepared phosphovanillin reagent and incubate in incubator shaker; (4) read absorbance at 530 nm and determine the lipid content by comparing to the standard curve.