Assertion: machupo virus's rna | /r/HasProperty | isolated
Facets:
value: from tissues
type: OTHER
value: by using the rnaid kit
type: MANNER
Source sentences:
text: For sequencing, the isolate was propagated in T75 flasks, virus particles in supernatant were pelleted by ultracentrifugation, and RNA was isolated by using the QIAamp Viral RNA kit (QIAGEN).
text: Cell debris was pelleted by centrifugation, and RNA was isolated from the homogenate with the RNeasy Mini kit (QIAGEN).
text: Total RNA was isolated by using the RNaid Kit following
text: (B) Cells overexpressing hTRIM2 or mTRIM2 or GFP (Control) were incubated with Candid 1, and after a 1-hr incubation at 37°C, virus was stripped from cells, and RNA was isolated and analyzed for viral RNA by RT-qPCR.
text: RNA was isolated from tissues by using an RNAqueous kit (Ambion), and cDNA was generated by using the SuperScript III First-Strand Synthesis system for RT-PCR (Invitrogen).
text: RNA was isolated from these tissues using RNAqueous (Ambion), and cDNA was generated by SuperScript III First-Strand Synthesis system for RT-PCR (Invitrogen).