Assertion: target nucleic acid | /r/HasProperty | isolated
Facets:
value: from the cleared solution
type: OTHER
value: preferably
type: DEGREE
value: from other cellular material
type: OTHER
Source sentences:
text: When the target nucleic acid is contained in the interior of a cell, the cell membrane must be disrupted and the contents of the cell released into the solution surrounding the cell before the target nucleic acid can be isolated from other cellular material.
text: For example, when the target nucleic acid is plasmid DNA being isolated from a cleared lysate solution, the plasmid DNA is combined with a matrix under conditions wherein the plasmid DNA forms a complex therewith while non-target materials, such as proteins, lipids, and chromosomal DNA remain in solution.
text: When the target nucleic acid is mRNA, it is preferably isolated from the cleared solution using a system which utilizes oligo(dT) to specifically adsorb to the poly(A) tail of mRNA (
text: Where the target nucleic acid contains a specific, unique sequence not shared by non-target nucleic acids, it can also be isolated using an oligonucleotide complementary to the sequence immobilized on a support surface (e.g. U.S. Pat.