Assertion: microtubule | /r/HasProperty | polymerized with 1 mm gtp
Facets:
value: from gmpcpp seeds
type: OTHER
value: for 10 min
type: TEMPORAL
value: at 37 °c
type: LOCATION
value: for sample preparation
type: PURPOSE
Source sentences:
text: For sample preparation, microtubules were polymerized from GMPCPP seeds with 1 mM GTP, 15 μM Tubulin, 20 nM mCherry-EB3 and in the absence or presence of 100 nM Fchitax-3 at 37 °C for 10 min (designated –Fchitax-3 and +Fchitax-3 sample, respectively).