Assertion: modified nucleotide | /r/Causes | possible steric hindrance
Facets:
Source sentences:
text: Since the incorporation of ddNTPs-R 2 - fluorophore into a strand of DNA permanently terminates further extensions of that template in the first approach and the incorporation and cleavage of C-F-NRTs leaves a tail of the modified nucleotide that causes possible steric hindrance to lower the incorporation efficiency of the subsequent base in the second approach, the total number of sequenceble templates decreases after each cycle of SBS reaction.