Assertion: intracellular nucleic acid | /r/HasProperty | eluted with 1 x pcr buffer
Facets:
value: from the membrane
type: LOCATION
Source sentences:
text: The membrane bound nucleic acids were washed with a wash buffer comprising 90% ethanol and eluted from the membrane with 1 x PCR buffer (50mM KCI, 3mM MgCI2, 10mM Tris pH 8.6 @ 25oC) and subjected to a multiplex qPCR designed to detect wt BRAF, BRAF V600E, and BRAF