Assertion: dynamic microtubule | /r/AtLocation | coverslip
Facets:
value: to prepare the rigor kinesin-1–coated coverslips
type: PURPOSE
Source sentences:
text: For the “seedless” assay, dynamic microtubules were prenucleated in solution with GTP and then attached to coverslips by surface-bound rigor kinesin-1 motor protein [6His-kinesin-1(R210A)]. To prepare the rigor kinesin-1–coated coverslips, silanized coverslips were first rinsed with BRB80 [80 mM piperazine-N,N′-bis(2-ethanesulfonic acid),