Assertion: immunoglobulin | /r/CapableOf | obtain the immunoglobulin
Facets:
value: by eluting the protein
type: MANNER
value: in the eluate
type: LOCATION
Source sentences:
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: To obtain the immunoglobulin, the immunoglobulin is then eluted and obtained in the eluate, which may then further be purified in a further chromatographic step, if desired.
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) either to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) either to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin; (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin; (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin; wherein the ligand of the Protein A affinity chromatography resin is alkali-stabilized Protein A derivative (e.g. MabSelect SuRe);
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin; wherein the ligand of the Protein A affinity chromatography resin is an alkali-stabilized Protein A derivative (e.g. MabSelect SuRe);
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; wherein the ligand is 4-Mercapto-Ethyl-Pyridine;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; wherein the ligand is 4-Mercapto-Ethyl-Pyridine;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; wherein the ligand is 4-Mercapto-Ethyl-Pyridine; (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; wherein the ligand is 4-Mercapto-Ethyl-Pyridine; (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; (b2) exposing the flow-through obtained in step (b) to Protein A affinity
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b2), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b2), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; wherein the ligands of the Mixed Mode
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b2), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b2), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b2), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b2), to cation exchange chromatography, wherein the immunoglobulin is bound to the to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin, wherein the ligand of the cation exchange chromatography resin is sulfopropyl.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin, wherein the ligand of the cation exchange chromatography resin is sulfopropyl.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin, wherein the ligand of the cation exchange chromatography resin is sulfopropyl.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, wherein the ligand of the Protein A affinity chromatography resin is an alkali-stabilized protein A derivative (e.g. MabSelect SuRe);
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin, wherein the ligand of the cation exchange chromatography resin is sulfopropyl.
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: (d) exposing the eluate obtained in step (c) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: (d) exposing the eluate obtained in step (c) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: (d) exposing the eluate obtained in step (c) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: (d) exposing the eluate obtained in step (c) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin.
text: (b) exposing the flow-through obtained in step (a) to immunoglobulin binding protein/peptide affinity chromatography, wherein the immunoglobulin is bound to the immunoglobulin binding protein/peptide affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the immunoglobulin binding protein/peptide affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: (d) exposing the eluate obtained in step (c) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin.
text: (a) exposing the sample to anion exchange chromatography and obtaining the immunoglobulin, which is not bound to the anion exchange chromatography resin, in the flow-through; (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (a) exposing the sample to anion exchange chromatography and obtaining the immunoglobulin, which is not bound to the anion exchange chromatography resin, in the flow-through; (b) exposing the flow-through obtained in step (a) either to Protein A affinity chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin, or to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin;
text: (c) exposing the eluate obtained in step (b) , or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;
text: (d) exposing the eluate obtained in step (c) to Mixed Mode chromatography, wherein the immunoglobulin is bound to the Mixed Mode chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Mixed Mode chromatography resin; wherein the ligand of the Mixed Mode chromatography resin is a multimodal strong anion exchanger.
text: chromatography, wherein the immunoglobulin is bound to the Protein A affinity chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the Protein A affinity chromatography resin;
text: (c) exposing the eluate obtained in step (b), or a composition derived therefrom and obtained after one or more further processing steps performed after step (b), to cation exchange chromatography, wherein the immunoglobulin is bound to the cation exchange chromatography resin, and obtaining the immunoglobulin in the eluate by eluting the protein from the cation exchange chromatography resin;