Assertion: dynamic microtubule | /r/HasProperty | prenucleated in solution
Facets:
value: with gtp
type: OTHER
value: for the “seedless” assay
type: PURPOSE
Source sentences:
text: For the “seedless” assay, dynamic microtubules were prenucleated in solution with GTP and then attached to coverslips by surface-bound rigor kinesin-1 motor protein [6His-kinesin-1(R210A)]. To prepare the rigor kinesin-1–coated coverslips, silanized coverslips were first rinsed with BRB80 [80 mM piperazine-N,N′-bis(2-ethanesulfonic acid),