Assertion: immunoglobulin | /r/MadeOf | solution
Facets:
value: to an anion exchanger
type: TRANSITIVE-OBJECT
value: onto the column
type: LOCATION
value: with a support
type: OTHER
Source sentences:
text: In one aspect, the invention provides a method for removing a thrombogenic agent from an immunoglobulin containing solution, the method comprising the steps of: providing the immunoglobulin containing solution at a pH in the range of higher than 3.8 to equal to or lower than 5.3; providing a support comprising immobilized negatively charged groups; contacting the solution with the support; and collecting an unbound fraction I.
text: It was found according to the present invention that carrying out a second cation exchanger chromatography step resulted in an increased removal of FXIa from the immunoglobulin containing solution as compared to carrying out a single cation exchanger chromatography step.
text: The support can be equilibrated prior to contacting the solution/fraction with a buffer e.g. by washing the support with the buffer of the immunoglobulin containing solution.
text: The term “un-bound fraction” typically refers to the flow through material obtained or collected following contacting the immunoglobulin containing solution with a support comprising charged groups or to the flow through material that is obtained or collected following contacting an immunoglobulin containing solution/fraction with a chromatography support.
text: The immunoglobulin containing solution can be subjected to a filtration step prior to chromatography in order to reduce aggregates in the solution.
text: In the case of column chromatography, an un-bound fraction can be obtained following washing of the loaded column with the same buffer used for equilibration and/or the buffer used for loading (oftentimes referred as to “binding buffer”) of the immunoglobulin containing solution onto the column.
text: In one embodiment of the invention, a FXIa removal of more than 75% e.g. 80%, 85%, 90%, 95% and 99% from the immunoglobulin containing solution or fraction as compared to the initial FXIa content (before contacting the solution or the fraction with the support comprising the immobilized negatively charged groups according to the invention) is considered efficient.
text: In one embodiment of the invention, a kallikrein removal of more than 75% e.g. 80%, 85%, 90%, 95%, 97% and 99% from the immunoglobulin containing solution or fraction as compared to the initial kallikrein content (before contacting the solution or the fraction with the support comprising the immobilized negatively charged groups according to the invention) is considered efficient.
text: It was surprisingly found that subjecting an immunoglobulin containing solution to an anion exchanger at a pH level range of 7-8.2 resulted in maximal removal of PKA and, practically, no loss of IgG.
text: The invention also provides a method for removing a thrombogenic agent from an immunoglobulin containing solution comprising the steps of: providing the immunoglobulin containing solution at a pH in the range of 7 to 8.2, providing a support comprising immobilized positively charged groups, and providing a support comprising immobilized negatively charged groups; contacting the solution with the support comprising the immobilized positively charged groups; collecting the un-bound fraction; adjusting the pH of the fraction to a pH in the range of higher than 3.8 to equal to or lower than 5.3; contacting the un-bound fraction with the support comprising the immobilized negatively charged groups; and collecting an unbound fraction I.
text: In one embodiment of the invention, detectable amounts of thrombosis-generating agents (e.g. PKA, kallikrein and/or FXIa) are removed from the immunoglobulin containing solution.
text: In one embodiment of the invention, a PKA removal of more than 80% e.g. 83%, 85%, 90%, 95% and 99% from the immunoglobulin containing solution or fraction as compared to the initial PKA content (before contacting the solution or the fraction with the support comprising the immobilized positively charged groups according to the invention) is considered efficient.
text: For example, the immunoglobulin containing solution can be subjected to solvent/detergent treatment, heat treatment, selective chromatography and nanofiltration.