Assertion: soluble melanin | /r/HasProperty | prepared in nonenzymatic reaction
Facets:
value: by mixing 5,6-dihydroxyindole-2-carboxylic acid
type: MANNER
Source sentences:
text: Soluble melanin can be prepared in a nonenzymatic reaction by mixing 5,6-dihydroxyindole-2-carboxylic acid (DHICA) and 5,6-hydroxyindole (DHI) in the presence of oxygen in a non-destroying buffer (e.g., 0.1 molar sodium phosphate, pH 6.5 to 7.5) or by incubating DHICA alone at ambient temperature of any suitable non-destroying temperature which allows the reaction to occur (e.g., 15 to 37° C.), until soluble melanin begins to appear (e.g., 3 to 6 hours).