Assertion: ligand | /r/CapableOf | lose biological activity
Facets:
value: because of steric hindrance between binding sites
type: CAUSE
value: by multipoint attachment
type: MANNER
Source sentences:
text: Coupling at low pH is less efﬁcient, but can be advantageous if the ligand loses biological activity when it is ﬁxed too ﬁrmly, for example, by multipoint attachment, or because of steric hindrance between binding sites which occurs when a large amount of high molecular weight ligand is coupled.