Assertion: immunoglobulin's fraction | /r/HasProperty | washed in 25 mm tris
Facets:
Source sentences:
text: Elution of the bound protein was monitored using the Bradford protein dye-binding procedure (Bio-Rad), and both unbound and protein A-bound fractions were concentrated and washed in 25 mM Tris and 192 mM glycine using Amicon concentrators (molecular mass cutoff of 10 kDa) prior to analysis of both of these fractions in the absence or presence of 5% β-mercaptoethanol by 5-12% SDS-PAGE.