Assertion: optic nerve | /r/HasProperty | followed by 30% sucrose
Facets:
value: at 4[degrees]c.
type: TEMPORAL
value: overnight
type: TEMPORAL
Source sentences:
text: Optic nerves were carefully dissected, placed in 4% PFA for 1 h, and transferred to 20% sucrose in 0.1 M PBS at 4[degrees]C overnight followed by 30% sucrose overnight at 4[degrees]C.