Assertion: ngf | /r/HasProperty | 100 ng/ml
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Source sentences:
text: The cultured islets were pretreated with K252a for 1 hour before adding NGF (100 ng/mL), and the mRNA levels were measured by quantitative RT-PCR after 3 h of NGF treatment.
text: C, D, Representative morphological changes of cultured islets in serum-starved RPMI1640 medium without (C) or with (D) NGF (100 ng/mL) for 3 days.
text: F, Representative fluorescent microscopic findings of islets cultured with or without NGF (100 ng/ml) for 24 hours.
text: The production of inflammatory cytokines was not influenced by the addition of NGF to murine cultured islets The effects of NGF (100 ng/ml) on IL-6 (A), IL-1β (B), and IL-10 (C) mRNA levels measured by quantitative RT-PCR after different durations of treatment (0, 3, 6, 9, and 12 h) are shown.
text: HUVEC were then seeded on the surface of polymerized Matrigel (5.104 cells/well) and cultured in the presence of NGF (100 ng/ml) or VEGF (10 ng/ml) for 18 h. Tubular networks in each well were photographed using Nikon Eclipse Ti-U inverted microscope before measurement of tubular lengths using NIS element Basic Research (Nikon).
text: When cells reached confluence, they were treated with NGF (100 ng/ml) or VEGF (10 ng/ml) in EBM/0.5% FBS for 6 h.
text: After 2 washes, HUVEC were treated with NGF (100 ng/ml) or VEGF (10 ng/ml) in presence or absence of L-NAME (Sigma) for 2 h.
text: The following day, HUVEC were cultured in 2 ml EBM/0.5% FBS and MDA-MB-231 in 2 ml serum-free MEM in the presence of NGF (100 ng/ml) for 6 h or 24 h.
text: Finally, NGF (100 ng/ml, 24 h) increased the secretion of the proinflammatory cytokines IL-1β ( P < 0.01) and tumor necrosis factor-α (TNF-α) ( P < 0.001) by control rat pulmonary arteries.
text: By contrast, in the presence of NGF (100 ng/ml, 1 h of pretreatment), pulmonary arterial contraction to phenylephrine (PHE) ( Figure 3A ) or to prostaglandin F 2α (PGF 2α ) ( Figure 3B ) was increased.