Assertion: chain immunoglobulin | /r/MadeOf | determined restriction site
Facets:
Source sentences:
text: a) treating a sample containing lymphoid cells, especially periferal, lymphocytes, spleen cells, lymph nodes or another lyphoid tissue from a healthy animal, especially selected among the Camelids, in order to separate the lymphoid cells, b) separating polyadenylated RNA from the other nucleic acids and components of the cells, c) reacting the obtained RNA with a reverse transcriptase in order to obtain the corresponding cDNA, d) contacting the cDNA of step c) with 5' primers corresponding to mouse V H domain of four-chain immunoglobulins, which primer contains a determined restriction site, for example an Xho I site and with 3' primers corresponding to the N-terminal part of a C H 2 domain containing a Kpn I site, e) amplifying the DNA, f) cloning the amplified sequence in a vector, especially in a bluescript vector, g) recovering the clones hybridizing with a probe corresponding to the sequence coding for a constant domain from an isolated heavy-chain immunoglobulin.
text: d) contacting the cDNA of step c) with 5' primers corresponding to mouse V H domain of four-chain immunoglobulins, which primer contains a determined restriction site, for example an Xho I site and with 3' primers corresponding to the N-terminal part of a C H 2 domain containing a Kpn I site,