Assertion: immunoglobulin's fraction | /r/HasProperty | collected
Facets:
value: more specifically
type: DEGREE
Source sentences:
text: In one embodiment, the pH of the immunoglobulin containing solution is adjusted with an acid or base solution to a pH in the range of 7 to 8.2 and the solution is contacted with the positively charged groups pre-equilibrated with a buffer or PuW having the same pH as the solution and the unbound fraction is collected.
text: The pH of the collected fraction can be adjusted with an acid solution to a pH in the range of higher than 3.8 and equal to or lower than 5.3 and the fraction can be contacted with the negatively charged groups pre-equilibrated with a buffer having the same pH as the fraction and the unbound fraction is collected.
text: In another embodiment, the pH of the immunoglobulin containing solution is adjusted with an acid or base solution to a pH in the range of higher than 3.8 and equal or lower than 5.3 and contacted with the negatively charged groups pre-equilibrated with a buffer having the same pH as the solution and the unbound fraction is collected.
text: The pH of the collected fraction can be adjusted with a base solution to a pH in the range of 7 to 8.2 and contacted with the positively charged groups pre-equilibrated with a buffer or PuW having the same pH as the fraction and the unbound fraction is collected.
text: In one embodiment of the invention, the immunoglobulin containing solution is first contacted with the support comprising the immobilized positively charged groups at a pH in the range of 7 to 8.2, the un-bound fraction is collected; then the un-bound fraction is contacted with the support comprising the immobilized negatively charged groups; and an un-bound fraction I is collected.
text: I” is collected; and then un-bound fraction I is contacted with the support comprising the immobilized positively charged groups and an “un-bound fraction” is collected.
text: More specifically, following contacting the solution with the support comprising the immobilized positively charged groups, the un-bound fraction is collected (the flow through material obtained following contacting the immunoglobulin solution with the support).
text: I”, or “un-bound fraction II” are contacted with the chromatographic material comprising the three-dimensional cross-linked hydrophobic acrylic polymer, and an unbound fraction III is then collected.
text: The unbound fraction is collected, the pH of the unbound fraction is equilibrated according to the invention before loading to a cation exchanger according to the invention, and the unbound fraction I is collected.
text: In one embodiment of the invention, the immunoglobulin containing solution is first contacted with the support comprising the immobilized positively charged groups (anion exchanger); the resulting unbound fraction is collected and contacted with the support comprising the immobilized negatively charged groups (cation exchanger) and the resulting unbound fraction I which comprises immunoglobulin composition with low thrombogenic agents is collected.