Assertion: neuroepithelium's cell | /r/HasProperty | washed with pbs
Facets:
Source sentences:
text: Cells were washed twice with PBS and fixed with 4% paraformaldehyde for 10 minutes at room temperature.
text: The cells were fixed in 4 % paraformaldehyde for 15 minutes at room temperature, washed in PBS, and exposed to the primary antibodies overnight at 4°C.
text: Cells were washed with PBS and resuspended in FACS buffer (PBS with 2% FBS).
text: After 24 h, the culture medium was refreshed, and 36 h later cells were washed with PBS and harvested.
text: Cells were washed with PBS and lysed in lysis buffer (40 mM HEPES pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 mM phenylmethylsulfonylfluoride, 1 mM sodium orthovanadate and 0.5 mM DTT) supplemented with phosphatase inhibitor and EDTA-free protease inhibitor cocktails (Roche).
text: Cells were washed twice with PBS and fixed with 4% paraformaldehyde for 10 minutes at room temperature.