Assertion: neuroepithelium's cell | /r/HasProperty | incubated with primary antibodies
Facets:
value: for 1 hour at room temperature
type: TEMPORAL
value: for overnight at 4°c
type: TEMPORAL
value: after blocking with pbs
type: TEMPORAL
Source sentences:
text: After blocking with PBS containing 10% normal serum (goat or donkey serum depending on the species of primary antibody; Sigma) and 0.1% Triton X‐100 for at least 30 minutes at room temperature, cells were incubated with primary antibodies for 1 hour at room temperature or overnight at 4°C.
text: Cells were incubated with secondary antibody (anti‐rabbit and anti‐mouse, Molecular Probes, Paisley, UK, invitrogen.com, 1:500) for 1 hour at room temperature.
text: After blocking with PBS containing 10% normal serum (goat or donkey serum depending on the species of primary antibody; Sigma) and 0.1% Triton X‐100 for at least 30 minutes at room temperature, cells were incubated with primary antibodies for 1 hour at room temperature or overnight at 4°C.