Assertion: l isoleucine | /r/HasProperty | isolated in preferred method
Facets:
value: by purifying the isoleucine ester
type: MANNER
Source sentences:
text: In a preferred method, the L-isoleucine is isolated from the recovered mixture of amino acid esters by subsequently purifying the isoleucine ester by distillation, hydrolyzing the isolated isoleucine ester by refluxing with water, aqueous acid or alkali, and crystallizing the released L-isoleucine by concentra­ting and neutralizing the resulting solution.