Assertion: dna polymerase | /r/MadeOf | incubated
Facets:
value: for 5 min
type: TEMPORAL
Source sentences:
text: M13 ssDNA (0.05 pmol), annealed with a 55 nucleotide long primer (5′-dTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC-3′), was mixed with each DNA polymerase (0.05 unit) and dNTP (0.2 mM) in a 20 μl solution, containing 20 mM Tris-HCl, pH 8.8, 5 mM MgCl 2 , and 14 mM 2-mercaptoethanol, and incubated 74°C for 5 min.