Assertion: embryonic fibroblast | /r/HasProperty | mefs
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text: Mouse embryonic fibroblasts (MEFs) are often used as "feeder cells" in human embryonic stem cell research.
text: Mouse embryonic fibroblasts (MEFs) derived from our Nkx2-1-mCherry knock-in reporter line30 were transduced with retroviruses containing the transcription factors Oct4,
text: However, transplantation of 107 mouse iLEC alone or the parental mouse embryonic fibroblasts (MEFs) alone did not form any cell/tissue mass that could be detected and harvested after 10 weeks (data not shown).
text: We introduced each of the 24 candidate genes into mouse embryonic fibroblasts (MEFs) from Fbx15embryos by retroviral transduction ().
text: Mouse embryonic fibroblasts (MEFs) play a vital role in stem cell research where they function as feeder cells to maintain mouse and human embryonic stem cells (ESC) or pluripotent stem cells (iPSC) in an undifferentiated state .
text: Beschreibung Mouse embryonic fibroblasts (MEFs) are used as a feeder layer when culturing mouse and human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
text: Embryos are a rich source of fibroblasts, and murine embryonic fibroblasts (MEFs) are frequently prepared to study the physiological consequences of selective gene ablations.
text: The genetically amenable mouse model has led to a large collection of genetically defined lines from which mouse embryonic fibroblasts (MEFs) have been derived.
text: Mouse embryonic fibroblasts (MEFs) have been a standard model system for biological research since the early 1970s.
text: The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1.
text: Here, we present an optimized method for the isolation and culture of mouse embryonic fibroblasts (MEFs), preparation of conditioned medium (CM) and enzyme-linked immunosorbent assay (ELISA) to assess the levels of Activin A within the media.
text: Embryonic stem cells (ESCs) modified with a construct carrying a puromycin-acetyltransferase and eGFP under the control of the α-myosin heavy chain promoter (cell line D3/αPIG44 ) were maintained on inactivated murine embryonic fibroblasts (MEFs) in IMDM supplemented with FCS (15%), penicillin (100 U/mL), streptomycin (100 μg/mL), nonessential amino acids (1%), 2-mercaptoethanol (0.1 mM), and Leukaemia Inhibitory Factor (1,000 U/mL).
text: Mouse embryonic fibroblasts (MEFs) have been used reliably as feeder cells for mouse embryonic stem cells (mESCs) since early 1980s when the first mESC lines were being derived, and cultivated.
text: To develop a chemical cocktail that enables fibroblast-to-chondrocyte conversion, we used a two-stage model for the induction of mouse embryonic fibroblasts (MEFs) based on published results showing that direct reprogramming is a stepwise process ( Figure 1 A) ().
text: (hES) cells are generally cultured as cell clusters on top of a feeder layer formed by mitotically inactivated murine embryonic fibroblasts (MEFs) to maintain their undifferentiated state.
text: Undifferentiated hESCs can be maintained and proliferated on mouse embryonic fibroblasts (MEFs) or human feeder cells.
text: Chemical induction of epithelial-like cells (ELCs) from mouse embryonic fibroblast (MEFs)
text: After transfection of four Yamanaka factors and manipulating pathways for cardiogenesis, mouse embryonic fibroblasts (MEFs) can be reprogrammed into cardiomyocytes with spontaneous contraction .
text: Here, we show that embryonic mouse neural stem and progenitor cells (NSPCs) have significantly higher capacity than mouse embryonic fibroblasts (MEFs) to maintain their chromosome stability in response to acute (γ−radiation) and chronic (tritiated thymidine -3H-T- incorporation into DNA) genotoxic stress.
text: Four days later, cells were passaged onto irradiated murine embryonic fibroblasts (MEFs).
text: Mouse embryonic fibroblasts (MEFs) were isolated and prepared from E13.5 embryos, mouse postnatal fibroblasts (NBFs) were from day 3 mice and mouse adult lung fibroblasts (MAFs) were from > 8 week old mice.
text: The authors compared iPSCs from different origins (mouse embryonic fibroblasts (MEFs) and several adult tissues), with or without Myc retroviral transduction, and with or without drug selection for the expression of pluripotency genes (Nanog and Fbxo15).
text: To accomplish this, we infected mouse embryonic fibroblasts (MEFs) with a vector carrying the mCherry gene driven by Cyp11a1 promoter (Cyp11a1 promoter-mCherry-MEFs).
text: CF-1 mouse embryonic fibroblasts (MEFs) were used as the feeder cells (Chemicon International, Temecula, CA).
text: Stem Cell Biology , Issue 64, Molecular Biology , Developmental Biology , mouse embryonic fibroblasts (MEFs)
text: Here, we present an optimized method for the isolation and culture of mouse embryonic fibroblasts (MEFs), preparation of conditioned medium
text: These fibroblasts have been widely used as feeder layers to support the growth and expansion of embryonic stem cells (ESCs); most commonly the mouse embryonic fibroblasts (MEFs) (Amit et al . 2004 ).
text: The required approval for isolation and culture of primary mouse embryonic fibroblasts (MEFs) was obtained from the Institutional Animal Ethics Committee (IAEC), Manipal University.
text: Expression of mesenchymal markers and differentiation potential of mouse embryonic fibroblasts (MEFs ) at P5 (A) reverse transcription–polymerase chain reaction (RT –PCR )
text: Isolation and growth kinetics of mouse embryonic fibroblasts (MEFs )
text: We used mouse embryonic fibroblasts (MEFs) harboring a DC-specific reporter system (Clec9a-Cre X R26-stop-tdT mouse, hereafter called Clec9a-tdT) to identify DC-inducing transcription factors.
text: One of the many services that MAPS provides is generating custom mouse embryonic fibroblasts (MEFs) and embryonic stem cells (ESCs) from your desired strain.
text: Mouse embryonic fibroblasts (MEFs) are often used as "feeder cells" in human embryonic stem cell research.
text: Here we examine whether murine embryonic fibroblasts (MEFs) can support the integration of purified murine ES cell-derived cardiomyocytes in a 3-dimensional tissue culture model based on a freezed-dryed collagen matrix with tubular structure.
text: Kolossov and coworkers have observed that engraftment of ESC-CM in a murine model of heart infarction may be improved by co-transplantation of murine embryonic fibroblasts (MEFs).
text: (B) Cross-section (300 μm thick) through a paraformaldehyde (PFA)-fixed PI-stained collagen sponge seeded with murine embryonic fibroblasts (MEFs) and green fluorescent protein (GFP)-expressing ESC-CMs 7 days after seeding at lower magnification.
text: Hanging drops (20 μL) were formed with single cell suspensions of murine embryonic fibroblasts (MEFs) alone (1,000 cells/drop), ESC-CMs alone (1,000 cells/drop), or admixtures of 200 MEFs and 1,000 ESC-CMs per drop.