Assertion: second generation | /r/HasProperty | exposed through juvenile stage
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text: As the level 5 test for ecotoxicology, the Japanese Ministry of the Environment initially developed a full–life cycle test using Japanese medaka ( Oryzias latipes ) in which the first generation (F1) was exposed from the fertilized egg stage, and the eggs laid by the F1 generation were collected to initiate the second generation (F2), which was exposed through the juvenile stage, under the Strategic Programs on Environmental Endocrine Disruptors ’98 (SPEED ’98) 2 .