Assertion: embryonic fibroblast | /r/HasProperty | mef
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text: (A–C) Mouse embryonic fibroblast (MEF ) (P5)‐mediated teratomas inmice on right side of mice, while saline showed no outgrowth on left side of body.
text: We showed that induced pluripotent stem (iPS) cells can be generated from mouse embryonic fibroblasts (MEF) and adult mouse tail-tip fibroblasts by the retrovirus-mediated transfection of four transcription factors, namely Oct3/4, Sox2, c-Myc, and Klf4 ().
text: As a control, we introduced GFP to mouse embryonic fibroblasts (MEF) with ecotropic retrovirus produced in PLAT-E packaging cells().
text: Human ES cells (hESCs) have been successfully cultured on a layer of mouse embryonic fibroblast (MEF) or human feeders [ 1 – 4 ] or under feeder‐free conditions, in which the cells are maintained on Matrigel or laminin in MEF‐conditioned medium (MEF‐CM)
text: Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors.
text: To generate clonal cell lines from individual H9 ES cells, 384 single cells were removed from these cultures and transferred individually to the wells of larger plates that contained non-dividing mouse embryonic fibroblasts (MEF) as feeder layers.
text: (A) Alkaline phosphatase (AKP) staining of E14 mouse embryonic stem cells (ESCs) cultured on human foreskin fibroblast (HFF), mouse embryonic fibroblast (MEF) without exogenous leukemia inhibitory factor (LIF) or MEF with LIF for one, two and three passages, respectively.
text: A/C (LmnA/C), and beta-actin (Actb) genes in hybrid clones 4 and 9 of the tef series and clones 2 and 5 of the taf series, and in E14Tg2aSc4TP6.3 cells (ESC) and embryonic fibroblasts (MEF).
text: We directly reprogrammed the mouse embryonic fibroblast (MEF) into osteoblast cells using different combinations of Yamanaka factors with human lim mineralization protein-3 (hLMP-3).
text: Cardiomyocytes were also plated on inactivated murine embryonic fibroblasts (MEF) and stained for α-actinin (red) 20 days later (F).
text: Human embryonic stem cell (hESC) lines are traditionally derived and maintained on mouse embryonic fibroblasts (MEF) which are xenogeneic and enter senescence rapidly.