Assertion: cyclopropane's strain | /r/HasProperty | e. coli rotb-sw
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Source sentences:
text: When the 500 mL culture was grown at 37 °C with an OD at 600 nm of ~0.3, 50 μL of 0.3 M aqueous solution of cysteine and 50 μL of 0.25 M ammonium ferrous sulfate were added, and then the culture was further grown at 37 °C until the OD at 600 nm reached ~ 0.6, the protein expression was then induced upon addition of 30 μL of 1 M IPTG followed by incubation at 16 °C for another 24 h. Similar procedures were applied for the expression of Swoo_2002 using the strain E. coli RoTB-SW