Assertion: chlorophyll's pigment | /r/HasProperty | extracted
Facets:
value: by grinding leaves
type: MANNER
value: specifically
type: DEGREE
value: subsequently
type: TEMPORAL
value: for 2 hr in total darkness
type: TEMPORAL
value: by grinding with stainless steel beads
type: MANNER
Source sentences:
text: Pigments were subsequently extracted by grinding with stainless steel beads as described in the Methods section.
text: Allow the pigments to be extraction for 2 hr in total darkness.
text: Pigments were extracted by immersing the leaves in pure acetone that was cooled to 4°C and the extracts were subsequently analyzed by HPLC (see the Methods section for detail).
text: Specifically, the pigments were extracted by grinding leaves in pure acetone with stainless steel balls and the extracts were separated from cell debris by centrifugation.
text: Chlorophyllide a was detected in all three species when pigments were extracted by homogenizing leaves in pure acetone at room temperature or by immersing leaves in 4°C pure acetone.
text: Among these species, the largest accumulation of chlorophyllide was observed with pea leaves when the pigments were extracted by immersing leaves in pure acetone at 4°C, which converted 20% of chlorophyll a to chlorophyllide a (Figure 8D).