Assertion: cytosol | /r/HasProperty | step
Facets:
Source sentences:
text: When polyubiquitylation is carried out in a permeabilized cell system in the presence of the ATP analogue AMPPNP, the modified chains remain associated with the ER membrane (D. Flierman, C. Shamu, V. Chou and T.A.R., unpublished observations), which indicates that another ATP-dependent step is required for their release into the cytosol (Fig. 1, step 3).