Assertion: caspase's effect | /r/HasProperty | determined
Facets:
value: by treating the cells
type: MANNER
Source sentences:
text: The effects of the general caspase inhibitor Z‐VAD‐fmk and the cathepsin B inhibitor Z‐FA‐fmk on β 2 m‐induced apoptosis in CCRF‐HSB‐2 cells were determined by treating the cells (1 × 10 4 /well in 96‐well plates) with or without 100 μM Z‐VAD‐fmk or Z‐FA‐fmk for 3 hr.
text: Meanwhile non-structural 3C of EV71 is a protease (Li et al., 2002), but it is not clear about the relationship of proteolytic activity and activating caspases of 3C, so the effect of proteolytic activity of 3C on the activation of caspases is determined through detecting the activity of caspase-3, caspase-8, and caspase-9 after transfection of 3C or 3C mutant without protease ability, and it is confirmed that compared to 3C, 3C mutant decreases the activity of caspase-3, caspase-8, and caspase-9, respectively (Figure 1E), but 3C mutant possesses the ability of binding caspase-8 and caspase-9 as well as 3C (Figure 1F).