Assertion: centromere's sequence | /r/HasProperty | flanked by ecorv sites
Facets:
value: in a puc18 backbone
type: LOCATION
Source sentences:
text: For DNA-fragment preparation, NEB Stable E. coli cells containing a plasmid with a multiple copy (20×) of the 147-bp Widom 601 sequence flanked by EcoRV sites in a pUC18 backbone (gift from F. Martino, MRC–LMB) were cultured in LB broth with ampicillin.