Assertion: centrosome's protein | /r/HasProperty | stacked in the top of a 4–12% nupage gel
Facets:
value: briefly
type: MANNER
value: before r-250 coomassie blue
type: TEMPORAL
value: before staining
type: TEMPORAL
value: before digestion
type: TEMPORAL
Source sentences:
text: Briefly, proteins were stacked in the top of a 4–12% NuPAGE gel (Invitrogen) before R-250 Coomassie blue (Bio-Rad) staining and in-gel digestion using modified trypsin (Promega, sequencing grade).