Assertion: centromere's nucleosome | /r/HasProperty | incubated with trypsin
Facets:
value: in 10 µl of reaction solution
type: LOCATION
Source sentences:
text: The purified nucleosome (0.38 μM), containing H3.1 or CENP-A, was incubated with trypsin (2.5, 5.0, 7.5, 10 ng, purchased from Sigma-Aldrich) in 10 µl of reaction solution, containing 8 mM Tris-HCl (pH 7.5), 0.5 mM MgCl 2 , and 1 mM DTT, at 25 °C for 1 min.