Assertion: centromere's region | /r/HasProperty | amplified by pcr
Facets:
value: with primers
type: OTHER
Source sentences:
text: a region spanning the almost the entire retrotransposon (but lacking the second LTR to avoid rearrangement or transposition problems in E. coli or S. pombe) was amplified by PCR with primers indicated in Supplementary Table 12 and cloned in two steps into the NotI-XbaI site of pK(5.6 kb)-MCS-ΔBam to make pK-Sj-Tj7-