Assertion: centrifugation | /r/CapableOf | remove large vesicles
Facets:
Source sentences:
text: This protocol includes centrifugation (500 ×g, 30 min; 12,000 ×g, 30 min) of the sample of a biological fluid (culture medium) to remove cells, debris, and large vesicles; then the supernatant is mixed with 0.1 volume of sodium acetate buffer (1.0 M pH 4.75) and incubated on ice for 30–60 min and additionally for 5 min at 37°C.