Assertion: chloroplast's genome | /r/HasProperty | supplementary fig
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text: The pairwise alignments with species close related to S. adstringens showed a high conservation of the general structure of the chromosome and correct arrangement of the chloroplast regions validating the proposed genome (Supplementary Fig.
text: The thermocycling conditions were denaturation at 98°C for 2 min, and 39 cycles of 98°C for 2 s and 60°C for 5 s. To analyze genome copy number, we designed two sets of primers for each genome (Supplementary Table 1) to improve the accuracy of quantification, and used the mean as the threshold cycle (Ct) value of each genome.
text: Besides canonical genes, we identified freestanding open reading frames (ORFs) showing similarities (E-value threshold of 1e-06) with recognized protein domains or previously reported ORFs of unknown function in four of the newly sequenced genomes (Supplementary Table S2).