Assertion: centromere's nucleosome | /r/HasProperty | incubated with his 6 -tagged pr-set7
Facets:
value: in 5.0 µl of reaction solution
type: LOCATION
Source sentences:
text: The purified nucleosome (0.48 μM), containing H3.1, CENP-A, H3.1CATD, or CENP-AQD, was incubated with His 6 -tagged PR-Set7 (0.15 or 0.30 μM) in 5.0 µl of reaction solution, containing 10 mM HEPES-KOH (pH 7.8), 8 mM Tris-HCl (pH 7.5), 20 mM KCl, 40 µM EDTA, 80 µM of S-adenosylmethionine, 50 mM NaCl, 0.5 mM DTT, and 9% glycerol, at 25 °C for 1, 2, 4, and 8 min.
text: The purified nucleosome (0.48 μM), containing H3.1 or CENP-A, was incubated with His 6 -tagged PR-Set7 (0.49, 0.97, 1.5, and 1.9 μM) in 5.0 µl of reaction solution, containing 10 mM HEPES-KOH (pH 7.8), 18 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.10 mM EDTA, 80 µM of S-adenosylmethionine, 50 mM NaCl, 0.50 mM DTT, and 15% glycerol, at 25 °C for 30 min.