Assertion: choroid's membrane | /r/HasProperty | incubated with secondary antibody
Facets:
value: for 2 h
type: TEMPORAL
value: after washing
type: TEMPORAL
Source sentences:
text: After washing, the membranes were incubated with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) secondary antibody (Dako, Glostrup, Denmark) for 2 h in PBS-T. Excess antibody was then removed by extensive washing, and bound antibody was detected with an ECL chemiluminescence kit (Amersham, Little Chalfont, UK).