Assertion: cytosol's protein | /r/HasProperty | boiled in 2× sds loading dye
Facets:
value: before subjecting to sds–page
type: TEMPORAL
value: for 10 min
type: TEMPORAL
Source sentences:
text: Proteins captured on the magnetic beads were boiled in 2× SDS loading dye for 10 min before subjecting to SDS–PAGE and western blotting.