Assertion: centromere's nucleosome | /r/HasProperty | incubated for 2 h
Facets:
value: at 4 °c
type: LOCATION
Source sentences:
text: The mixed mono-nucleosomes were incubated for 2 h at 4 °C with 45 μl of Dynabead-conjugated protein G (Invitrogen), which was pre-incubated with 5 μg of mouse anti-H4K20me1 and 2.5 μg of rabbit anti-H2Av (Spike-in antibody; Active Motif) in buffer B [20 mM Tris-HCl (pH 8.0), 5 mM EDTA, 500 mM NaCl, 0.2% Tween 20, and cOmplete protease inhibitor cocktail