Assertion: annealing's temperature | /r/HasProperty | low
Facets:
Source sentences:
text: This temperature must be low enough to allow for hybridization of the primer to the strand, but high enough for the hybridization to be specific, i.e., the primer should bind only to a perfectly complementary part of the strand, and nowhere else.
text: This can only occur once the temperature of the solution has been lowered.
text: This lower energy state is the result of a slow process of cooling the material from a high temperature (i.e. high energy level) towards lower temperature (i.e. low energy level).
text: If the temperature is too low, the primer may bind imperfectly.