Assertion: antigen's antibody | /r/HasProperty | added
Facets:
Source sentences:
text: The primary antibody with an attached (conjugated) enzyme is added, which binds specifically to the test antigen coating the well.
text: (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form. .
text: (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form. .
text: (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form.
text: (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form.
text: A specific antibody is added, and binds to antigen (hence the 'sandwich': the antigen is stuck between two antibodies).
text: The secondary antibody, specific to the primary antibody, is added.
text: -Afterwards, a secondary antibody is added, which will bind to the antibody bound to the test antigen in the well.
text: The support is washed to remove all unbound molecules, and an antibody to the beta subunit is added.