Assertion: antibody's potential | /r/CapableOf | destabilize the igm
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Source sentences:
text: Given the large mass of IgM (960 kDa) molecules, their inherent instability in the harsh conditions used for IgG purification, and their lack of binding to Protein A and Protein G, purifying IgM tends to be more difficult than purifying IgG. When designing purification methods for IgM, the properties of IgM that must be taken into consideration include (1) the narrow range of pH under which IgMs are typically soluble (pH 6–8 for many IgMs); (2) their large mass and thus slow diffusion, which affects column washing, capacity, and discrimination when using media such as cross-linked agarose and similar porous particles; (3) the potential to destabilize or denature the IgM upon exposure to various conditions that are normally used to purify IgGs or other large proteins; and (4) the tendency for IgM to aggregate, necessitating additional steps to provide stable, monomeric IgM such as the addition of low percentages of polyethylene glycol.