Assertion: bound antibody | /r/HasProperty | detected
Facets:
value: by adding substrate
type: MANNER
Source sentences:
text: The bound antibodies were detected using goat anti-human IgG conjugated with HRP (horseradish peroxidase) (Southern Biotech) and TMB (3,3′,5,5′-tetramethylbenzidine) substrate (ThermoFisher Scientific).
text: The bound antibody was detected by adding substrate (25 µL of 1-Step Ultra TMB-ELISA, 34028, Thermo Fisher Scientific), quenching after 20–40 min (25 µL of 2 M H 2 SO 4 ) and measuring the absorbance values at 450 nm (Tecan Safire2 plate reader).