Assertion: crude azadirachtin | /r/HasProperty | 8.5 g.
Facets:
Source sentences:
text: Prod., 50 242, 1987) have described isolation of azadirachtin from neem seed kernels involving the following steps; (1), neem seed kernels (2.0 kg.) were ground with hexane (21.) in a commercial Waring blender (10 min.) to a fine powder and allowed to settle for 1 h., agitated again and filtered under vacuum to give a neem seed kernel extract residue; (2), the procedure described in step 1 is repeated four times successively resulting in a defatted neem seed kernel cake which is extracted with 95% ethanol (21.), five times by the procedure described in step 1 by percolation within the blendor for 8-12 h.; (3), the combined ethanol extracts after concentration in vacuum yielded a dark viscous residue (185 g.); (4), the ethanol extract residue from step 3, was partitioned between hexane and methanol/H 2 O, 95/5; (5), the 95/5 methanol/water phase (138 g.) was partitioned with water and ethyl acetate successively; (6), the ethylacetate phase (59 g.) was subjected to silica gel filtration with ethylacetate to give a product (52 g.) enriched in azadirachtin; (7), the product (52 g.) from step 6 was subjected to vacuum liquid chromatography (EtOAc/hexane, 3/1) yielding azadirachtin fractions (13 g.); (8) crystallisation of azadirachtin fractions from carbon-tetrachloride gave crude azadirachtin (8.5 g.); (9), flash chromatography of the product from step 8 (CHCl 3 /CH 3 CN, 3/1) gave azadirachtin (5 g.).
text: Prod., 50 242, 1987) have described isolation of azadirachtin from neem seed kernels involving the following steps; (1), neem seed kernels (2.0 kg.) were ground with hexane (21.) in a commercial Waring blender (10 min.) to a fine powder and allowed to settle for 1 h., agitated again and filtered under vacuum to give a neem seed kernel extract residue; (2), the procedure described in step 1 is repeated four times successively resulting in a defatted neem seed kernel cake which is extracted with 95% ethanol (21.), five times by the procedure described in step 1 by percolation within the blendor for 8-12 h.; (3), the combined ethanol extracts after concentration in vacuum yielded a dark viscous residue (185 g.); (4), the ethanol extract residue from step 3, was partitioned between hexane and methanol/H 2 O, 95/5; (5), the 95/5 methanol/water phase (138 g.) was partitioned with water and ethyl acetate successively; (6), the ethylacetate phase (59 g.) was subjected to silica gel filtration with ethylacetate to give a product (52 g.) enriched in azadirachtin; (7), the product (52 g.) from step 6 was subjected to vacuum liquid chromatography (EtOAc/hexane, 3/1) yielding azadirachtin fractions (13 g.); (8) crystallisation of azadirachtin fractions from carbon-tetrachloride gave crude azadirachtin (8.5 g.); (9), flash chromatography of the product from step 8 (CHCl 3 /CH 3 CN, 3/1) gave azadirachtin (5 g.).
text: 95/5; (5), the 95/5 methanol/water phase (138 g.) was partitioned with water and ethyl acetate successively; (6), the ethylacetate phase (59 g.) was subjected to silica gel filtration with ethylacetate to give a product (52 g.) enriched in azadirachtin; (7), the product (52 g.) from step 6 was subjected to vacuum liquid chromatography (EtOAc/hexane, 3/1) yielding azadirachtin fractions (13 g.); (8) crystallisation of azadirachtin fractions from carbon-tetrachloride gave crude azadirachtin (8.5 g.); (9), flash chromatography of the product from step 8 (CHCl 3 /CH 3 CN, 3/1) gave azadirachtin (5 g.).