Assertion: antibody's antigen | /r/HasProperty | diluted to 5 μg/ml
Facets:
value: with carbonate/bicarbonate coating buffer
type: OTHER
Source sentences:
text: Briefly, 100 μL of ePDPN-His fusion protein as coating antigen diluted to 5 μg/mL with carbonate/bicarbonate coating buffer was added into each well of the ELISA plate, and incubated at 37 °C for 2 h. Plate was washed and blocked with 5% PBSM (200 μL/well), and incubated at 37 °C for 2 h.