Assertion: capture antibody | /r/HasProperty | pab
Facets:
Source sentences:
text: Different concentrations of B. cereus whole-cell suspensions ranging from approximately 104 to 106 cells/mL were determined by a sandwich ELISA according to the following protocol: (1) the micro wells of the ELISA plate were coated with 100 μL of capture antibody (pAb) at 18 μg/mL in coating buffer at 4 °C overnight; (2) three washes with PBS-T were performed to remove unbound antibody and each well was blocked with 200 μL of 0.05% skim milk powder and incubated at 37 °C for 90 min; (3) the washing step was repeated and100 μL of ten-fold serial diluted samples (B. cereus) were added to each well at 37 °C for 40 min; (4) washes were repeated and 100 μL of the monoclonal antibody 2D2 at a 1:1000 dilution in PBS was added to each well and incubated at 37 °C for 30 min; (5) washing was repeated and100 μL goat anti-mouse IgG horseradish peroxidase conjugate (at a 1:5000 dilution in PBS) was then added to each well and was incubated at 37 °C for 30 min; (6) the plate was then washed six times and 100 μL of TMB solution was added to each well; (7) plates were incubated at 37 °C for 15 min; the reaction was then terminated by the addition of 50 μL of 2 M H 2 SO 4 and the absorbance values at 450 nm were measured.