Assertion: specific antibody | /r/HasProperty | immobilized on high binding elisa plates
Facets:
value: at 4°c
type: TEMPORAL
value: for 1–2 h
type: TEMPORAL
value: principally
type: MANNER
value: overnight
type: TEMPORAL
value: by incubation
type: TEMPORAL
Source sentences:
text: Principally, specific antibody is immobilized on high binding ELISA plates by incubation overnight at 4°C or for 1–2 h at 37°C, and then followed by 3–5 washes with PBST (137 mM NaCl, 2.7 mM KCl, 8.1 mM Na 2 HPO 4 , 1.5 mM KH 2 PO 4 , and 0.5% Tween 20;