  Phytochemical composition of ethanol leaf extract of Diodella sarmentosa was
profiled with GC-MS and the inhibitory property of the extract against total
microbial dehydrogenases were assessed. The major constituents of the extract
were squalene (29.50%), Phytol (24.68%), phenol, 3-pentadecyl- (18.58%),
1-Butanol, 3-methyl- (9.09%) and n-Hexadecanoic acid (7.78%). The minimum
inhibitory concentration (MIC) of the extract against broad spectrum of
microbial population was assessed. Bacillus subtilis, Candidas spp, and
Penicillium spp were more sensitive to the treatment and thus; were further
investigated using Dehydrogenase activity assay method. Total dehydrogenase
activities of Bacillus subtilis, Candidas spp, and Penicillium spp at the
extract concentration range of 0 to 2000mg/ml were progressively inhibited at
increasing extract concentrations. The threshold inhibitory concentrations
(IC50) of the extracts against Candidas spp, Penicillium spp and Bacillus
subtilis were 275micro. g/ml, 322 micro. g/ml and 411 micro. g/ml respectively.
Our findings suggested the extract as a useful source of antimicrobial
phytochemicals for pharmaceutical use.
