  In this paper, we focus on the relationship between the fifth Painlev\'{e}
equation and a Jacobi weight perturbed with random singularities,
\begin{equation*} w(z)=\left(1-z^2\right)^{\alpha}{\rm
e}^{-\frac{t}{z^2-k^2}},~~~z,k\in[-1,1],~\alpha,t>0. \end{equation*} By using
the ladder operator approach, we obtain that an auxiliary quantity $R_n(t)$,
which is closely related to the recurrence coefficients of monic polynomials
orthogonal with $w(z)$, satisfies a particular Painlev\'{e} V equation.
