  We present an electro-optic wide-field method to enable fluorescence lifetime
microscopy (FLIM) with high throughput and single-molecule sensitivity.
Resonantly driven Pockels cells are used to efficiently capture nanosecond
image dynamics on standard camera sensors. Images are gated at 39 MHz with 16
dB extinction and 86$\%$ modulation depth, allowing lifetimes to be estimated
with sensitivity 1.9 times the shot-noise limit and without restricting photon
throughput. High throughput is shown by acquiring FLIM images with millisecond
exposure and $>10^8$ photons per image. High sensitivity is shown through
wide-field lifetime imaging of single-molecule populations and dynamics. Using
both the throughput and sensitivity of the technique, we enable the first
combination of wide-field lifetime imaging with single-molecule localization
microscopy. We further demonstrate lifetime imaging of single-molecule FRET and
multi-parameter imaging of molecular binding events combining intensity,
lifetime, and polarization. More generally, resonant electro-optic imaging can
provide lifetime contrast in any wide-field method and will enable nanosecond
time-domain measurements in microscopy.
