Patent Document:

the following examples are given to illustrate the present invention but are not to be construed as limiting . dehydrated paprika ( 5 . 5 % moisture ) is ground in a hammer mill and the resulting ground paprika ( 95 % passing us 40 mesh ) is admixed with about 10 % by weight of soy bean oil and processed in a countercurrent extraction system involving three ( 3 ) pressing stages , each using an egon keller model kek - 100 screw press , with the extracts from the second and third stages being returned to the preceding mixing stage before being removed from the process at the end of the first press stage . a high shear , high speed pin mixer or equivalent is used to mix the soy oil or extracts from the second and third press stages into the ground spice or residual solid from the preceding stage . this recycling is continuous . the raw material paprika solids are continuously fed at a rate of about 240 lbs . per hour with a total contact time in each mixing stage of about 15 - 60 seconds . the residence time in each press is 5 - 60 seconds . the pressing stages are operated at about 10 , 000 psi internal pressure and about 200 degrees fahrenheit , which is maintained by cooling with water through the bore of the press shafts . the starting color value of the ground paprika solids is 200 asta . the principal components extracted and standardized in both the extract and the residual solid are the carotenoid pigments . the resulting final soy - paprika extract has a color value of about 1 , 375 asta and the reground paprika residual solid from the final ( 3rd ) press stage has a color value of about 85 asta . by varying the percentage of edible solvent employed from about 5 % to 20 %, the pressure from about 6 , 000 to 30 , 000 psi , the number of countercurrent mixing and pressing stages from 2 to 5 , with return of the extract from each press stage to the preceding mix stage before final removal from the process in the first press stage , varying the temperature from about 130 ° f . to 280 ° f ., and removing the seed from the paprika solids prior to grinding , the resulting extract ranges in color value from about 2 , 700 asta to about 800 asta and the residual solids range in color value from 180 asta to 35 asta . by regrinding the residual solids ( from the final stage ) just as is done with fresh , dehydrated paprika , a product in every way comparable to commercially available ground paprika solids is produced . after filtering or centrifuging off the fine particulate solids , the extract can be directly substituted for commercially available paprika oleoresin in every respect . by varying the pressing temperature of the process from about 130 ° f . to 325 ° f ., the hue of the reground residual solid is varied from slightly browned to a dark chocolate brown , demonstrating that the degree of brownness can be controlled by the pressing temperature employed . the degree of &# 34 ; brownness &# 34 ; is measured using a hunter labscan spectrocolorimeter with 0 degree illumination , 45 degree circumferential viewing , illuminant d65 , 10 degree observer , ceilab coordinate system . the hue of the paprika powder is measured by placing the powder in a 2 . 5 - inch diameter cuvette , shaking gently to ensure even coverage , and measuring through the bottom of the cuvette . the results of the varied operating temperatures of the process are shown in table i . the designation l * is indicative of the &# 34 ; lightness &# 34 ; of the sample with the higher numbers being lighter or less browned , and the lower numbers being darker or more browned . table i______________________________________processing temperature visual appearance l * values______________________________________130 ° f . red 40 . 18150 ° f . tan - red 37 . 25200 ° f . light brown red 33 . 22280 ° f . dark brown red 29 . 16325 ° f . chocolate red 22 . 85______________________________________ the data clearly demonstrate that the degree of browning can be controlled by varying the press temperature at which the process is conducted . this broadens the applications or uses of the residual solid to include a base for toasted chili powder and as a replacement for browned , caramelized paprika . the residual solid can be substituted for ground paprika or chili powder in many common applications and a separate processing step for browning to a desired degree is not required . the starting ground paprika solids have an aerobic plate count ( analysis run according to bacterial analytical manual by aoac , 8th edition , 1995 , and iso - grid methods manual , 3rd edition , 1989 ) of about 14 , 000 , 000 . the residual solids exiting the extraction system have a count of about 2 , 000 to 200 , 000 , with the lower count being achieved at the higher temperatures . this is a significant reduction and makes the residual solids per se suitable for any application where treatment with ethylene oxide or irradiation would normally be required . the foregoing example is repeated with all materials and conditions being the same , except that the soybean oil edible solvent is supplemented with an antioxidant blend at a concentration of 3 % by weight of the original ground paprika solids . the blend consists of about 29 % lecithin , 20 % powdered ascorbic acid , 5 % citric acid , 15 % tocopherol , and 1 % rosemary extract ( in accordance with chang and wu u . s . pat . no . 5 , 077 , 069 ). the stability of ( 1 ) the resulting extract and ( 2 ) the residual solids is compared in each case with an untreated control . in such evaluation , the paprika extracts are plated on flour salt to an extent of 2 . 4 % by weight with a mortar and pestle . two - gram samples are weighed into 13 × 100 mm test tubes . the test tubes are stored in a thermostatically - controlled oven at 65 ° c . samples are withdrawn periodically , extracted with acetone , and the color at 460 nm of a standard (%) dilution in acetone is determined spectrophotometrically . in the evaluation of the residual solids , two - gram samples of the reground residual solid are substituted for the flour salt dispersions . the procedure for the &# 34 ; standard dilution &# 34 ; is as follows : the initial color of the dispersion is determined by pouring two grams of the original dispersion into a 100 - ml flask . acetone is added up to the 100 - ml level . the flask is inverted several times . the flour salt is allowed to settle for five minutes . then three ml of the dilution is pipetted into a 25 - ml flask and diluted up to the 25 - ml level . the absorbance is read at 460 nm . the 460 nm color is determined by the formula : ## equ1 ## where the percent dispersion is determined by the formuls : ## equ2 ## to translate to asta color , multiply the 460 nm color by 820 . the color is plotted against time and the time for 1 / 3 of the starting color to fade is reported as the 2 / 3 life . this is a highly - reproducible measurement , which is sufficiently accurate to evaluate the effectiveness of the antioxidants and will assist the practitioner to optimize formulations for specific uses . the final extract from the first press stage of the unprotected or unstabilized process has a color value of about 1375 asta and a 2 / 3 life of 6 . 5 hours as compared to a color value of about 1600 asta and a 2 / 3 life of 63 hours for the extract from the protected material . the color value of the unprotected or unstabilized residual solids is about 85 asta with a 2 / 3 life of 54 hours , compared to the protected solids which have a color value of about 95 asta and a 2 / 3 life of 155 hours . this clearly demonstrates that inclusion of antioxidants can improve not only the color yields from the extraction process but also at the same time improve the color stability of both the extract and the residual solids . other suitable antioxidants ( e . g ., lecithin , ethoxyquin , butylated hydroxy anisole ( bha ), butylated hydroxy toluene ( bht ), tertiary butyl hydroxy quinone ( tbhq ), sesame , tea catechins , and labiatae herb antioxidant activity , finely - divided ascorbic acid , tocopherol , citric acid ) can be substituted in whole or in part for the specific antioxidant mixture employed with similar desirable color - protective results , preferably a naturally - occurring antioxidant from an herb of labiatae family , e . g ., rosemary , sage , or thyme , or powdered ascorbic acid . dehydrated paprika solids ( 2 . 5 % moisture ) were ground in a hammer mill and the resulting ground paprika ( 95 % passing us 40 mesh ) was processed with about 15 % by weight of soy bean oil in a countercurrent extraction system as in example 1 involving two ( 2 ) pressing stages , with extracts from the second press stage being returned to the preceding ( first ) mix stage before being removed from the process at the first press stage . upon exiting the first press stage , distilled water was metered continuously into the crude extract at a rate of 75 % by weight of the gums and solids by means of an inline static mixer . the weight of the gums and fine particulate solids in the extract was determined by diluting one gram of the crude extract in nine grams of acetone . the mixture was spun down for three minutes at 2000 g &# 39 ; s in a laboratory centrifuge . the solids separated were air dried and the weight of the gums and solids was calculated as a percentage of the weight of the starting extract . the hydrated gums and solids removed from the extract were continuously returned to the final residual press solids via a high shear , continuous pin mixer installed immediately following a water - jacketed cooling screw which received the residual solids from the second press stage . prior to hydration and centrifugation , the extract contained approximately 10 % by weight of gums and fine particulate solids as determined by the above - described method . following hydration and centrifugation the gums and particulate solids amounted to no more than 1 % by weight of the extract and the extract was a crystal clear solution , free of any suspended insoluble materials . the color value of the starting ground paprika was about 150 asta . the pressing stages were operated at about 20 , 000 to 30 , 000 psi . the extraction process was started with the presses operating at about 80 ° f . as measured by the temperature of the cake exiting the presses . the temperature of the presses was controlled by the rate of flow of cooling water through the bore of the press shafts and the screen cages to keep the operating temperatures in the range of 80 ° to 180 ° f . over the time of the extraction run , the operating temperatures of the presses , as measured by the temperature of the cake exiting the presses , was gradually increased to about 255 ° f . by first slowing and then stopping the flow of cooling water to obtain operating temperatures of 180 - 200 ° f ., and then by substituting steam for the water in the shaft and cages at gradually increasing pressures to achieve temperatures of 200 - 255 ° f . samples of the extracted oil and press residual solids were pulled at various temperature intervals as the temperatures were increased . samples of the residual solids were pulled at two points , the first ( non - rehydrated ) immediately after exiting the cake - cooling screw following the final ( second ) pressing stage , and the second after the thus - cooled residual press solids were rehydrated to a moisture content of about 10 %. the samples were assayed for asta color , aerobic and anaerobic plate count , and color stability over time using methods employed in examples 1a and 1b . the advantages of operating the process at a temperature above 130 ° f ., as indicated by the temperature of the cake exiting the presses , can clearly be seen . the plate count of both the extract and the cake are progressively reduced as the temperatures are increased . ( tables 2 & amp ; 3 ) table 2______________________________________effect of increasing temperatureson the plate count of the extracttemperature degree f . aerobic plate count anaerobic plate count______________________________________ 80 1 , 900 , 000 790 , 000130 1 , 700 , 000 800 , 000150 1 , 700 , 000 660 , 000170 1 , 600 , 000 500 , 000175 1 , 500 , 000 425 , 000180 1 , 300 , 000 380 , 000190 360 , 000 150 , 000200 300 , 000 200 , 000215 240 , 000 150 , 000225 190 , 000 65 , 000235 170 , 000 32 , 000245 69 , 000 8 , 600255 3 , 800 830______________________________________ table 3______________________________________effect of increasing temperatures on the platecount of the press solidstemperature degree f . aerobic plate count anaerobic plate count______________________________________ 80 220 , 000 55 , 000130 160 , 000 35 , 000150 160 , 000 25 , 000170 100 , 000 20 , 000175 32 , 000 15 , 000180 80 , 000 7 , 400190 3 , 500 800200 9 , 800 3 , 400215 5 , 800 2 , 300225 4 , 100 500235 1 , 900 1 , 100245 5 , 400 100255 800 100______________________________________ the efficiency of extraction is dramatically improved as evidenced by the progressively decreasing asta values and the progressively decreasing residual extractable yields of the press residual solids . it is apparent that , to achieve residual extractable yields of less than about 20 % by weight of the cake , it is necessary to operate the presses at 130 ° f . or higher . ( table 4 ) moreover , for obvious reasons of efficiency , temperatures above 180 ° f ., and especially between about 180 ° f . and about 235 ° f ., are greately preferred . table 4______________________________________press cake asta and residual yields atprogressively increasing temperaturestemperature degree f . press solids asta press solids residual yield______________________________________ 80 87 28 . 28 % 130 76 16 . 40 % 150 65 15 . 72 % 170 61 15 . 72 % 175 53 12 . 36 % 180 43 13 . 88 % 190 42 10 . 84 % 200 44 10 . 72 % 215 41 9 . 96 % 225 39 9 . 50 % 235 33 9 . 28 % 245 32 9 . 00 % 255 35 9 . 80 % ______________________________________ most importantly , the stability of the extract is not adversely affected and is in fact increased . the results , from example 2 , of an accelerated study on the stability of the extract , generated at varying press operating temperatures , can be seen in table 5 . the accelerated study was done according to the procedures described in example 1b with the colors reported as a percent of the starting color for each respective sample to adjust for the varying color yields at the respective temperatures . these results demonstrate that the extract produced at higher operating temperatures exhibits increased resistance to oxidative color deterioration . this is surprising , as explained in the following . table 5______________________________________press oleoresin ( extract ) stability , accelerated , 65 ° c . temperature hour hour hour hour hourdegree f . 2 4 8 12 17______________________________________ 80 94 % 88 % 81 % 73 % 62 % 130 94 % 89 % 82 % 75 % 64 % 170 93 % 89 % 82 % 76 % 65 % 225 94 % 90 % 82 % 78 % 67 % 235 94 % 90 % 82 % 77 % 69 % 255 95 % 90 % 84 % 78 % 72 % ______________________________________ it is commonly believed that lipid - containing systems , exposed to heat , will exhibit an increased rate of lipid oxidation that , once initiated , will proceed at an ever - increasing rate . ( rancidity and its measurement in edible oils and snack foods , a review , robards , kerr , and patsalides , analyst , february 1988 , vol 113 ). in fact , prior art ( u . s . pat . no . 4 , 681 , 769 ) claims a process for counter - current , high pressure extraction of capsicums at less than 100 ° f . and less than 500 psi for the express reason of protecting the extracted oil from oxidation . to confirm the positive effect of high temperature treatment in more controlled conditions , a forty gram sample of hexane - extracted oleoresin paprika , with no diluents added , was heated in a beaker on a heated stir plate at 100 ° c . for eight and one - half hours . a control sample which was unheated , a sample pulled from the heated beaker after four hours , and a sample of the material heated for the full eight and one - half hours were dispersed on flour salt to make dispersions of 1 . 2 % oleoresin by weight of flour salt . two gram - portions of the dispersions were weighed into test tubes and placed in a 65 ° c . oven . an initial asta color was run on each dispersion and then asta colors were run periodically and the results were plotted versus time to determine the relative stability of the heated and unheated samples . the results are shown in table 6 . it can be readily observed that the heat - treated samples , although they lose some initial color during the heating process , have improved stability over time , thus confirming the improved resistance to oxidation observed in table 5 . table 6______________________________________asta values of heated & amp ; unheated oleoresinpaprika ( extract ) over timehours unheated heated 4 @ 100 ° c . heated 8 @ 100 ° c . ______________________________________ 0 32 . 5 31 . 5 26 . 0 2 29 . 0 29 . 0 25 . 8 4 26 . 0 28 . 0 25 . 7 6 24 . 0 27 . 0 25 . 5 8 22 . 5 25 . 8 25 . 310 21 . 0 24 . 5 25 . 012 20 . 0 23 . 0 24 . 814 19 . 0 22 . 3 24 . 516 18 . 0 21 . 8 24 . 018 17 . 0 21 . 0 23 . 520 16 . 0 20 . 0 23 . 022 15 . 1 19 . 0 22 . 524 14 . 2 18 . 5 22 . 126 13 . 4 18 . 0 21 . 828 12 . 9 17 . 5 21 . 430 12 . 5 17 . 0 21 . 0______________________________________ the non - rehydrated press residual solids produced in example 2 exhibit decreased resistance to oxidative color loss as the press operating temperatures are increased as predicted by prior art ( bennett et al , u . s . pat . no . 4 , 681 , 769 ) and as seen in table 7 . table 7______________________________________stability of non rehydrated press solids at variouspress operating temperatures , expressed as percentof starting color retainedtemperature degree f . week 2 week 4 week 6______________________________________ 80 86 . 7 % 82 . 2 % 85 . 5 % 130 89 . 6 % 85 . 5 % 84 . 6 % 170 73 . 3 % 65 . 3 % 58 . 1 % 225 61 . 7 % 35 . 8 % 32 . 5 % 245 68 . 2 % 31 . 0 % 19 . 3 % ______________________________________ but , very importantly , it can be seen that the press residual solids which are rehydrated immediately after exiting the second press stage of the process ( example 2 ) exhibit significantly increased stability ( table 8 ) relative to the non - rehydrated solids , thus overcoming the claimed disadvantages from operating at temperatures above 100 ° f . as set forth in u . s . pat . no . 4 , 681 , 769 . table 8______________________________________stability of rehydrated press solids at variouspress operating temperatures , expressed as percentof starting color retainedtemperature degree f . week 2 week 4 week 6______________________________________ 80 90 % 92 % 91 % 130 93 % 91 % 92 % 170 92 % 92 % 91 % 225 94 % 93 % 91 % 245 95 % 94 % 93 % ______________________________________ in fact , after discounting for the effect on pigment stability of increasing residual extractable yields in the press solids ( tables 4 & amp ; 11 ) obtained at the lower temperatures , the carotenoid pigments in the residual solids would show enhanced stability for a given residual extractable yield . these are surprising and unexpected results and clearly overcome the supposed obstacle of operating at elevated press temperatures and pressures . it is further surprising that the color stability of the residual press solids is significantly improved by controlling the water activity ( a w ) of the solids in ranges above those suggested for the stabilization of lipidcontaining systems by extensive studies and particularly by nelson and labuza , water activity and food polymer science : implications of state on arrhenius and wlf models in predicting shelf life , k . a . nelson & amp ; t . p . labuza , journal of food engineering 22 , 271 - 289 ( 1994 ). water activity is defined as the ratio of the vapor pressure of water in a food to the vapor pressure of pure water at the same temperature . prior art suggests that maximum stability of lipid systems should be attained at water activities of about 0 . 3 with decreasing stability developing as the water activity is increased above this level . in this example we find precisely the inverse effect on stability of the carotenoid pigments for a given water activity . in order to confirm the effect of high temperatures in the pressing operation , and to confirm the effect of added moisture , a controlled test was performed on a laboratory scale where the effect of levels of extractable yield in the cake could be controlled to eliminate the effect of variable press cake residual yields on the stability of the carotenoids . a 3 , 000 gram sample of ground paprika solids ( 175 asta , 9 . 8 % extractable yield ) was dried in a lab tray dryer at 100 ° f . for 16 hours to a moisture content of about 2 %. one half of this sample was then heated in an oven at 220 ° f . for twenty minutes to approximate the temperature in a pressing operation according to the invention . the other unheated sample served as a control . one hundred gram samples of each of the two materials were rehydrated at approximately 1 % intervals up to about 12 % moisture . the water activity a w of each was determined using a rotronics hygroskop dt , model dt2 / 1 - 00iv , water activity instrument . samples were weighed into sealed test tubes , stored at ambient temperatures of about 72 ° f . in the dark , and the asta colors were determined over a period of eighteen weeks to determine the relative rates of color degradation . the color retained ( as a percentage of the starting color for each sample to compensate for the effect of color dilution with the rehydration water ) was plotted against time . table 9______________________________________percent color retained of unheated ground paprikaat various water activity rangeswater activity a . sub . w week 1 week 5 week 18______________________________________0 . 15 74 % 57 % 42 % 0 . 30 50 % 45 % 12 % 0 . 40 68 % 50 % 43 % 0 . 60 83 % 68 % 55 % ______________________________________ table 10______________________________________percent color retained of heated ground paprikaat various water activity rangeswater activity a . sub . w week 1 week 5 week 18______________________________________0 . 15 66 % 56 % 41 % 0 . 30 60 % 50 % 45 % 0 . 40 80 % 62 % 57 % 0 . 60 98 % 82 % 78 % ______________________________________ it can be seen in tables 9 & amp ; 10 that the stability of the carotenoid pigments follows almost precisely the inverse of the curve predicted by nelson & amp ; labuza ( fig2 ). it can also be seen from these tables that controlled temperature ( with concurrent browning ) significantly enhances the stability of the carotenoids above a water activity of 0 . 3 and particularly in the water activity range of 0 . 4 to 0 . 6 . water activity ranges higher than 0 . 6 were not tested as levels marginally higher than this range will support microbial growth which is not acceptable in a dry spice product . it can be concluded that the stability of the carotenoid pigments found in capsicums unpredictably does not follow the commonly - accepted and predicted pattern for lipid oxidation with respect to temperature and water activity as suggested in u . s . pat . no . 4 , 681 , 769 , or in the cited literature ( nelson and labuza , water activity and food polymer science : implications of state on arrhenius and wlf models in predicting shelf life , k . a . nelson & amp ; t . p . labuza , journal of food engineering 22 , 271 - 289 ( 1994 ); rancidity and its measurement in edible oils and snack foods , a review , robards , kerr , and patsalides , analyst , february 1988 , vol 113 ); describing the stability of lipid systems . in fact , high temperature treatment , combined with rehydration of the press solids to a water activity above 0 . 3 , preferably of 0 . 4 to 0 . 6 , significantly improves stability rather than decreases it . this is a very surprising and unpredicted result . it is well known that the lipid profile of capsicum and its extracts , without the addition of any diluents , comprises a mixture of saturated and unsaturated fatty acids , 60 - 70 % being unsaturated linoleic and linolenic , lipid and antioxidant content of red pepper , daood , biacs , et al ., central food research institute , budapest , hungary ( 1989 ) and the nature of fatty acids and capsanthin esters in paprika , nawar et al ., journal of food science , vol . 36 ( 1971 ). in fact , daood et al suggest that &# 34 ; . . . the presence of triglycerides containing high amounts of unsaturated fatty acids may be an important factor contributing to the fading of paprika during processing and storage .&# 34 ; the present findings are just the opposite . without in any way being limited by theoretical considerations , it is hypothesized that the presently - discovered surprising and unpredicted inverse relationship shown ( in tables 9 & amp ; 10 ) between the stability of carotenoid pigments at given water activities is due to the fatty acids in the substrate being preferentially attacked by the oxidation reaction at the low ( from about 0 . 05 to 0 . 2 a w ,) and higher water activity ranges ( above 0 . 3 , preferably about 0 . 4 to 0 . 6 a w ,), thus protecting the carotenoids . at the intermediate water activity ranges ( 0 . 2 to 0 . 4 a w ,), where the lipids are best protected , the carotenoids are more readily and preferentially attacked and exhibit low resistance to oxidative degradation . another controlled test was conducted to demonstrate the effect of different extractable yields in the residual solid press cake . the effect of higher amounts of unsaturated fatty acids is evident from the results illustrated in table 11 where fresh , refined , bleached , and deodorized soybean oil with no antioxidants was added at various percentages based on the weight of the paprika . the color over time was compared to the untreated control in an accelerated study at 65 ° c . a typical refined , bleached , and deodorized soy oil has a fatty acid composition of 22 . 3 % oleic ( 18 : 1 ), 51 % linoleic ( 18 : 2 ), and 6 . 8 % linolenic ( 18 : 3 ). ( riecel &# 39 ; s handbook of industrial chemistry , 9th edition , pg 278 ). it can be concluded that higher levels of unsaturated fatty acids , such as oleic , linolenic , and linoleic , which are found in most vegetable oils , will improve the color stability of the press residual solids . levels of extractable yield in the residual solids above about 15 - 20 % by weight of the residual solids is undesirable as the residual capstcum solids become difficult to handle for most uses and the efficiency of extraction is reduced , i . e ., less color can be removed from the spice as the residual yield is allowed to increase by decreasing either the pressure or temperature employed . table 11______________________________________percent color retained with varying amountsof soy oil added to ground paprikapercent addition hour 2 hour 4 hour 6 hour 8______________________________________ 0 % 65 % 59 % 52 % 50 % 5 % 90 % 83 % 74 % 72 % 10 % 92 % 84 % 75 % 74 % 15 % 94 % 87 % 80 % 78 % 20 % 96 % 91 % 83 % 81 % ______________________________________ it is readily apparent , comparing the results of the controlled test ( tables 9 & amp ; 10 ) on stability of heated vs unheated material , where oil is controlled at a constant level that , at a given added soy oil content in the press residual solids , the color stability of the residual press solids is significantly improved when the capsicum has been exposed to higher temperatures . this conclusion is not readily apparent in the results shown in table 8 where the amount of residual vegetable oil left in the press residual solids is higher in the low temperature ranges due to the decreased efficiency of the extraction process at lower temperatures ( table 4 ). the presence of higher amounts of residual oils there offers some protection which overshadows the increased protective effect at higher temperatures so evident in tables 9 & amp ; 10 . it can therefore be concluded that much , if not all , of the protection offered by operating the presses at temperatures lower than 100 ° f . ( as claimed in u . s . pat . no . 4 , 681 , 769 ) as compared to temperatures above 100 ° f . is simply due to the higher residual oil levels ( reduced extraction efficiency ) and that , for any given residual oil content , and with rehydrated residual solids , the operating temperatures above 130 ° f . give superior results , not only in an increased extraction efficiency which allows for a continuous , high speed process with increased throughput rates and significantly reduced microbial activity , but most surprisingly in an increased color stability of both the extract and the residual press solids , particularly when the press solids are rehydrated . as can be seen in table 4 , the press solids residual yield is much higher at temperatures below 100 ° f . and much higher ( 28 . 3 % residual yield ) than disclosed in u . s . pat . no . 4 , 681 , 769 ( 10 - 15 % residual yield ). in example 2 , table 4 , the model kek 100 screw press used for the test was operated at about 100 % of its rated capacity of 240 pounds per hour for typical oil seeds . in an effort to more closely model the residual yields of 10 - 15 % ( oil ) in the cake as disclosed in u . s . pat . no . 4 , 681 , 769 , the feed rate for this test was set at about 95 pounds per hour , thus allowing more residence time in the press to expel more extract and to reduce the residual yield of the press residual solids to 10 - 15 %. the following example according to bennett is a two - step production run . one lot , comprising about 300 lbs of 160 asta chili , ground to pass 20 mesh ( usss ), was transferred to a ribbon blender and blended with 13 . 7 % by starting weight of the ground chili of fortified soybean oil having 500 asta oleoresin for about 15 minutes and then allowed to stand for about 16 hours at room temperature ( 75 ° f .) before transfer to the feed hopper of an egon keller model kek - 100 screw press . the feed hopper provides for a controlled flow of the mixture of chili and fortified oil to the press at a rate of about 95 lbs per hour of fresh ground chili , the equivalent of about 800 lbs per hour in a french oil mill machinery company f - 44 press . both these feed rates represent about 40 % of the rated capacity of the respective screw presses on whole oil seeds . the production run was started with a cone setting of about 0 . 030 inches and with the internal worms configured so as to provide a pressure gradient of essentially little or no pressure up to about 500 pounds per square inch of pressure . at these low pressures and feed rates , effluent oil temperatures were maintained at less than 100 ° f . with cooling water as in bennett , and the residual yield ( oil ) in the press residual solids averaged about 12 . 5 %, just as prescribed by bennett , who states that : &# 34 ; temperatures above 100 ° f . should be avoided inasmuch as higher temperatures cause oxidation with a resultant destruction of delicate flavor and / or color principle .&# 34 ; with the press operating as described , the oil extracted , after centrifugation to remove the residual spice fines , assayed at about 1000 asta and the press cake residual solids fraction had a corresponding reduction in asta to about 115 . the press cake of the once - extracted ground fresh spice from the first pressing is further processed following the same procedure above described for the first blending / pressure extraction sequence using , however , fresh soybean oil as an additive in place of the fortified oleoresin soybean oil . the fortified soybean oil extracted assayed at approximately 500 asta . this 500 asta fortified soybean oil extract is recycled as an extractant on fresh ground chili . the extracted chili powder cake from this extraction step had a corresponding reduction in asta value to an average of about 65 asta ( ranged from 41 to 95 asta ). the results of this low temperature , low pressure test are compared to results of high temperature , high pressure conditions in example 2 and are shown in table 12 . table 12______________________________________comparison of low temperature / low pressure and hightemperature / high pressure low temp / low high temp / high pressure , 16 hour pressure continuous batch mixing from example 2______________________________________temperature , deg f . 95 235pressure , psi & lt ; 500 20 , 000 - 30 , 000final residual solids asta 65 33final residual solids yield 12 . 5 % 9 . 3 % final residual solids asta as a 41 . 5 % 22 % percent of fresh chili astaasta loss in 1st mixing stage 7 % 0 % asta loss in 2nd mixing stage 10 . 5 % 0 % final oleoresin asta 1 , 000 1 , 000______________________________________ it can clearly be seen , as is also shown in example 2 ( effect of varying operating temperatures ), that the low temperature / low pressure batch process with extended contact times incurs significant color loss during the extended contact times necessary for low temperature / pressure extraction . in addition , the low temperature / pressure batch process does not remove the color as efficiently as with higher temperatures and pressures for any given size pressing operation . the foregoing example can scientifically be scaled up or extrapolated into a comparative two - stage production run using two model 44 - f french oil mill machinery company presses , as follows : one lot comprising about 3 , 840 lbs of 5 % moisture , 160 asta , 20 mesh , fresh ground chili solids ( capsicum annum ) is passed through a high speed , high shear , steam - jacketed paddle mixer on a continuous basis and fed directly into the press feed hopper at stage one , through the 1st stage press , into a 2nd stage paddle mixer , and then to the 2nd stage press . soybean oil is added continuously through a metering pump into the paddle mixer at stage 2 at the rate of 13 . 7 % by weight of the starting ground chili solids ( 525 lbs of oil for the 3840 lb run ). the raw material chili solids are continuously fed at a rate of about 2 , 500 lbs per hour to the system with a total contact time in each paddle mixer of about 15 seconds . the temperature of the chili / oil mix exiting the paddle mixer is maintained at about 180 ° f . at stage 2 and about 150 ° f . at stage 1 . the oil / extract expelled from the second pressing stage is returned on a continuous basis to the paddle mixer at stage one wherein the oil / extract and fresh ground paprika are mixed in preparation for the first pressing stage . the oil / extract and fresh ground paprika exit the first stage paddle mixer and enter the first stage press at about 150 ° f ., the temperature being controlled by the amount of steam on the paddle mixer jacket . the concentrated oil / extract expelled from the first pressing stage is hydrated with water to about 75 % by weight of the fines ( fine particulate solids ) and gums and is then centrifuged and the hydrated fines and gums are added to the residual press solids from the final ( second ) pressing stage in a high shear mixer , after the solids have passed through a water - jacketed cooling screw . the internal shaft and collar arrangements of the press are configured so as to provide internal pressures of about 20 , 000 to 30 , 000 psi and cooling water is maintained at a flow rate through the bore of the shaft and through the cage cooling jackets so as to maintain an exit oil temperature at the external cage surface of about 180 to 200 ° f . and an exiting residual solid cake temperature of about 235 ° f . the residual cake solid is cooled in a water - jacketed screw conveyor to about 85 ° f . and water , in addition to the hydration water used to remove the fines and gums from the extract , is injected into the high shear , continuous mixer to rehydrate the cake to a water activity of about 0 . 6 . the concentrated extract exiting press stage one has an asta value of about 1 , 000 and the residual press solid cake exiting stage two has an asta value of about 45 . the residual press solid cake has a reddish - brown appearance typical of lightly toasted chili powder . the aerobic plate count of the residual solid cake is about 70 , 000 . the same test is repeated ( according to the bennett example ). no heat is applied during the mixing stages and the press internals are reconfigured so as to provide for minimal friction and compression and the resultant minimal heat generation during the pressing operations . the working compression is supplied primarily by the cone at the cake discharge and is maintained at about 500 psi . oil is added at a rate of about 13 . 7 % by weight of the starting paprika solids ( 525 lbs for the 3840 lb batch ) and mixed in a ribbon blender for sixteen hours and then fed at ambient temperature ( about 75 ° f .) to the pressing system . the feed rate through the pressing stages is maintained at 800 pounds per hour . cooling water is supplied to the internal bore of the shafts and the cooling jackets to maintain exit oil temperatures of less than 100 ° f ., on both the expelled oil and the residual press cake . the extract exiting the first pressing stage of the press is centrifuged without hydration of the gums and fine particulate solids . the concentrated extract exiting press stage one has an asta value of about 1 , 000 and the residual cake solid has an asta value of about 65 . the appearance of the cake is lacking the brownness characteristic of commercially - available ground paprika and chili powder and would require a separate browning step to make it acceptable for common uses . the material is difficult to regrind due to the high level of residual extractable yield left in the cake , it is not flowable , and it must be combined with other solid materials to make an acceptable product for sale . the aerobic plate count is about 220 , 000 . a comparison of the results of the two tests is shown in table 13 . table 13______________________________________ high temperature low temperature pressing pressing______________________________________mixing time , soy oil continuous , 16 hours ( seconds ) fresh oil temp , ° f . 75 75oil / chili stage 1 at press feed , 150 75 ° f . oil / chili stage 2 at press feed , 180 75 ° f . cake after stage 1 pressing , ° f . 225 95cake after stage 2 pressing , ° f . 235 95asta value , extract 1000 9952 / 3 life , extract , 65 ° c ., hours 25 16asta value , press cake 45 652 / 3 life , cake , 72 ° f ., weeks 32 10color recovery , extract 75 % 51 % aerobic plate count , extract 70 , 000 2 , 000 , 000aerobic plate count , cake 2 , 000 220 , 000overall color recovery ( extract 98 % 91 % and cake ) visual appearance , cake red - brown red - tanthroughput rate , lbs per hour 2 , 500 800______________________________________ it is readily apparent that there are substantial advantages to higher temperatures and pressures . the color recovery is enhanced , there is a 50 % increase in yield to the extract , the rate for a given press size is increased by over 300 %, the color stability of the extract is improved by 65 %, the color stability of the residual solids cake is improved by 300 %, and the aerobic plate count is reduced by a factor of greater than 30 in both the extract and the residual cake ; all without the oxidative color losses that are alleged to be an obstacle in u . s . pat . no . 4 , 681 , 769 . it is thereby seen that an improved countercurrent process for the extraction of capsicum solids using an edible solvent , whereby improved yields of both extract and residual solids are obtained , whereby both the extract and the residual solids have improved color stability and freedom from bacterial contamination due to the higher temperatures employed , whereby due to optional advantageous rehydration of residual solids and level of water activity employed an improved color stability in the residual solids is attained , whereby an extract in the form of a clear solution can be obtained by removal of gums and particulate solids in the form of their insoluble hydrates , whereby even greater color stability can be effected by the employment of edible antioxidants in the solvent utilized , and whereby controlled browning of the residual solids may be conveniently effected , all without the expected disadvantages of employing higher temperatures as clearly indicated by the prior art , and whereby all of the stated objects of the invention have been accomplished , has been provided . it is to be understood that the invention is not to be limited to the exact details of operation , or to the exact compositions , methods , procedures , or embodiments shown and described , as obvious modifications and equivalents will be apparent to one skilled in the art , and the invention is therefore to be limited only by the full scope which can be legally accorded to the appended claims .

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