Patent Document:

the treatment for bacterial and viral infections in cattle , horses , pigs , sheep and other domestic and non - domestic animals of the present invention is comprised in a liquid state of trace minerals , cobalt amino acid chelates , acidophilus sp ., kelp and vitamins in a solution using distilled water as the carrier . trace mineral elements have been linked to immune system health , cell growth enhancement , glucose tolerance factor and other overall health concerns . furthermore , metal ions have been shown to have certain antimicrobial properties . the above mentioned trace elements , when combined in the current formulation have an unexpected synergy in treatment of a diseased animal . the second solution used to formulate the new treatment composition is composed of a bacterial species that would recolonize the gastrointestinal tract as well as a nutritional formulation to provide needed vitamins and minerals to the affected animal . in addition , kelp , a natural source of carbohydrates , amino acids , vitamins , minerals and trace elements is also added . kelp contains over 60 minerals and elements including iodine , 21 amino acids , simple and complex carbohydrates . it is believed to be a promoter of glandular health , especially for the pituitary , adrenal and thyroid glands . the thyroid and pituitary glands regulate certain functions of digestion . kelp also provides a natural source of fiber . after the two solutions are mixed , an oral dose of approximately 10 - 15 ml . of the resultant treatment solution is administered to the affected animal . the dose is repeated every 24 hours until symptoms are relieved . after the animal responds to the treatment , and regains its appetite , a regular diet and feeding schedule can be resumed . furthermore , with the instant treatment for bacterial , fungal and viral infections in cattle , horses , pigs , sheep and other domestic and non - domestic animals composition , there is no requirement for isolation and identification of the causative agent or antimicrobial sensitivity testing of etiologic agents in order to determine antibiotic resistance . in addition , because the present composition does not incorporate synthetic antibiotics such as penicillin or amoxycillin , there is no selection for antibiotic resistant strains of bacteria which would necessitate concurrent or sequential administration of antibiotics . the present composition was administered on the above - noted 24 hour cycle to several test groups of infected animals . there was no mortality among those animals treated with the present treatment for bacterial and viral infections . affected animals that were treated with the present composition were asymptomatic after 1 to six days of treatments . a field trial was conducted where 15 yearling calves , all diagnosed with pneumonia , were each treated with 10 milliliters of the present treatment for bacterial and viral infections . the dose was administered orally once a day for up to 5 days . of these treated calves , 33 % were asymptomatic within 24 hours after having been given only one dose of the present composition . eight calves were asymptomatic within 48 hours having been given 2 doses . the other two calves were asymptomatic after 4 dosages . a second field trial included one limousin bull diagnosed with pneumonia .. the normal weight of the bull was 1 , 950 pounds but at the time of treatment , the weight was 1 , 625 pounds . the bull had experienced a weight loss of 325 pounds since the onset of symptoms . the bull would not eat or drink , had a severe dry cough with no discharge from the nose , was weak and lethargic . the bull was administered an oral dosage of 30 milliliters of the present composition . within one hour , he was eating hay and drinking water . he was asymptomatic within 3 days . on the seventh day , still asymptomatic , he was given a 30 milliliter follow up dose . the bull fully recovered and regained his normal weight . he was being used in a registered limousin breeding program . a third field trial was conducted on two hereford crossbred calves . the 4 month old calves were diagnosed with coccsidiosis . the symptoms included profuse , watery green stool with mucous and large amounts of bright red blood . both calves were weak . one calf , # 178 , had these symptoms for seven days without treatment and was steadily getting worse . the other calf , # 162 , had been sick for 3 days . both calves were given oral doses of 15 milliliters of the novel treatment for bacterial and viral infections once a day for two days . after the first administration , both calves were in a much improved condition . both were asymptomatic within 48 hours after having received a total of two doses of the present composition . a fourth field trial was conducted on 23 horses in a herd of 165 . the horses showing symptoms had deep rumbling cough , profuse green / yellow mucous discharge from both nostrils , dull glassy eyes , lowered heads , and were lethargic and despondent . the infected horses had displayed these symptoms for 7 to 20 days . symptoms continued to worsen with continued duration of the disease . all 23 horses were given 15 milliliters of the current composition once a day . nine of these horses were asymptomatic after 48 hours and two doses . ten horses were asymptomatic within four days having been administered three doses . four horses were asymptomatic within six days having been given five doses . a fifth field trial was conducted on a hereford crossbred cow . the cow had been diagnosed with foot rot in her left hind foot . she could not put any weight on it . the foot was swollen twice the normal size and red in color between the toes and around the comet band . there was a hole in the sole of the left toe at the heel , ½ inch in diameter , which was oozing putrefied matter . the cow was given an oral dose of 30 milliliters of the present composition . in addition , the novel treatment compound was administered topically around the cornet band and 10 milliliters was injected into the hole in the sole of the left toe using a needle - less syringe . within four days , the cow was walking on that foot . at that time , the oral and topical administration was repeated using the same protocol . within seven days of the second administration , the cow was fully recovered . these field tests demonstrated that the current composition for treatment infections in animals worked on a broad range of pathogens with no recurrence of symptomology . all animals recovered in a very short period of time with bright eyes , shiny coats , good appetites and vigorous carriage . in addition to the field trials conducted with the present composition for treatment of disease in animals , minimum inhibitory concentrations of the composition were determined for several bacteria and one mycotic organism . the protocol for performing this test is found in the national committee for clinical laboratory standards 9nccls ) publication m7 - t2 . the tests were conducted by larry w . harris , registered microbiologist rm ( n . r . m .) 3053 . the minimum inhibitory concentration ( mic ) measures the ability of the antimicrobial agent to inhibit multiplication of the organisms being challenged . thus , organisms in the innoculum are inhibited from replicating by the antimicrobial agent . examples of bacteriostatic agents are chloramphenicol and erythromycin , nalidixic acid , sulfonamides and tetracyclines . dilutions of the present composition were made in a nutrient broth . the nutrient broth is known to support growth of the organisms used to challenge the efficacy of the composition . a one to ten , a one to one hundred dilution and a one to one thousand dilution of the present composition were made in the nutrient broth . further dilutions of each concentration were made as follows in table 1 below : the undiluted antimicrobial composition and 1 : 10 , 1 : 100 and 1 : 1000 dilutions were each diluted as indicated in the chart above resulting in a set of 11 tubes for each . all of the tubes then received , one milliliter ( ml ) of nutrient broth . at this point each tube contained 2 ml . a bacterial suspension was added to each tube . 0 . 1 ml of the test organism suspension 1 × 10 6 cfu / ml , where cfu is an abbreviation for colony forming units , was added to each tube . each tube contained 2 . 1 milliliters of liquid at this point with a concentration of 2 . 5 × 10 4 cfu / ml . of the challenge innoculum . this procedure was conducted using four bacteria as the challenge innoculum . two gram negative organisms , psuedomonas aeruginosa and k - 12 escherichia coli were used as challenge organisms . two gram positive organisms , staphyloccocus aureus , and bacillus subtilis were used as challenge organisms . the tubes were then incubated at 35 ° c . over night . the tubes that were turbid were evaluated to have growth of bacteria . the nonturbid tubes were determined to be negative for growth of bacteria ( see table 2 below ). after overnight incubation of the tubes , 0 . 001 ml from the control tube and each of the non - turbid tubes was subcultured to nutrient agar , mannitol - salt agar and macconkey agar ( see table 3 , table 4 and table 5 below ). these subcultures were incubated at 35 ° c . overnight . cfu on subcultures were determined . additionally , 0 . 001 ml of the broth from control tubes were subcultured to agar resulting in a count of 250 colonies . in addition , efficacy of the present composition against mycotic organisms was tested . the minimum inhibitory concentration ( mic ) of the present composition was established using the previously described protocol . again , the minimum inhibitory concentration is that concentration of the compound which successfully prevents multiplication of the organisms being challenged . a one to ten , a one to one hundred dilution and a one to one thousand dilution of the present composition were made in the sabouraud dextrose broth . sabouraud dextrose broth is known to support growth of mycotic organisms used to challenge the efficacy of the composition . the results are illustrated in table 7 below . further dilutions of each concentration were made as follows in table 6 below : the undiluted antimicrobial composition and 1 : 10 , 1 : 100 and 1 : 1000 dilutions were each diluted as indicated in the chart above resulting in a set of 11 tubes for each . all of the tubes then received , one milliliter ( ml ) of nutrient broth . at this point each tube contained 2 ml . a suspension of the test organism was added to each tube . 0 . 1 ml of the test organism suspension 1 × 10 6 cfu / ml , where cfu is an abbreviation for colony forming units , was added to each tube . each tube contained 2 . 1 milliliters of liquid at this point with a concentration of 2 . 5 × 10 4 cfu / ml . of the challenge innoculum , in this case , saccharomyces cerevisiae . the tubes were then incubated at 35 ° c . over night . the tubes that were turbid were evaluated to have growth of the organism . the nonturbid tubes were determined to be negative for growth of the organism . after overnight incubation of the tubes , 0 . 001 ml from the control tube and each of the non - turbid tubes was subcultured to sabouraud dextrose agar . these subcultures were incubated at 35 ° c . overnight . cfu on subcultures were determined . additionally , 0 . 001 ml of the broth from control tubes were subcultured to agar resulting in a count of 250 colonies . the results are illustrated in table 8 below . the treatment for bacterial and viral infections in cattle , horses , pigs , sheep and other domestic and non - domestic animals of the present invention is comprised in a liquid state of 22 constituents . these constituents are formulated in two liquid solutions which are then combined and stored in liquid form . the composition is readily available in this form for administration to the animal at 10 - 15 ml per dose most commonly at 24 hour intervals . the first solution is composed of trace minerals in microgram amounts . the following is a list of trace minerals with the approximate amount of each in micrograms ( mcg ): cobalt 200 mcg copper 200 mcg silicon 120 mcg neodymium 100 mcg praseodymium 100 mcg nickel 70 mcg chlorine 65 mcg zinc 62 mcg yttrium 60 mcg strontium 15 mcg titanium 15 mcg aluminum 15 mcg chromium 10 mcg gallium 10 mcg rubidium 10 mcg total trace minerals 1052 mcg the trace organic minerals are suspended in a liquid carrier prior to final formulation of the new treatment composition . the following are the constituents of the second solution suspended in 1 oz . of water : acidophulus sp . 3 . 0 grams water ( distilled ) 1 . 0 liquid ounce dried kelp 1 . 0 gram folic acid 1 . 0 gram vitamin a supplement 0 . 5 grams vitamin d 3 supplement 0 . 5 grams cobalt amino acid chelates 0 . 2 grams all ingredients are mechanically mixed to form the liquid composition which may then be administered orally to affected animals in doses of 10 to 15 ml . the ratio of group 1 to group 2 is 24 : 1 respectively . a needle - less syringe is used to administer the required dosage into the back of the affected animals mouth . this treatment is repeated every 24 hours as needed . in severe cases , the dosage can be increased to 15 to 30 ml every 24 hours until the afflicted animal is asymptomatic . it should be understood , however , that even though these numerous characteristics and advantages of the invention have been set forth in the foregoing description , together with details of the composition and method of application of the invention , the disclosure is illustrative only , and changes may be made in detail , especially in matters of chemistry , dosage and implementation within the principal of the invention to the full extent indicated by the broad general meaning of the terms in which the appended claims are expressed .

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