Question: how to prepare 1.5 m tris hcl?
Answer: 1.5 M Tris, pH 8.8 (stock buffer for separating gels) For 1 L • Dissolve 181.65 g Tris base in around 800 mL of ddH2O Page 3 • Adjust the pH to 8.8 with concentrated HCl • Bring up the volume to 1 L with ddH2O Note: Make sure to let the solution cool down to room temperature before making the final pH adjustment.