Question: how do taqman assays work?
Answer: TaqMan genotyping assays are used to amplify and detect specific alleles in genomic DNA (gDNA). ... The exonuclease activity of AmpliTaq Gold® DNA Polymerase cleaves only probes hybridized to the target. Cleavage separates the reporter dye from the quencher dye, increasing fluorescence by the reporter.