Question: what is the difference between glycolysis and pyruvate oxidation?
Answer: Pyruvate is produced by glycolysis in the cytoplasm, but pyruvate oxidation takes place in the mitochondrial matrix (in eukaryotes). ... A carboxyl group is removed from pyruvate and released as carbon dioxide. The two-carbon molecule from the first step is oxidized, and NAD+ accepts the electrons to form NADH.