Question: how many molecules of atp are produced per molecule of glucose in glycolysis?
Answer: Overall, the input for 1 glucose molecule is 2 ATP, and the output is 4 ATP and 2 NADH and 2 pyruvate molecules. In cells, it is critical that NADH is recycled back to NAD+ to keep glycolysis running. Without NAD+ the payoff phase will halt and cause a backup in glycolysis.