Question: who killed ryuko matoi father?
Answer: Satsuki Kiryūin! Ryūko in Episode 1. Ryūko Matoi (纏 流子, Matoi Ryūko?) is the daughter of Isshin Matoi and the main protagonist of Kill la Kill. She transferred to Honnōji Academy searching for the twin of her red Scissor Blade and the person who used it to murder her father.