Question: how to dissolve plga nanoparticles?
Answer: I have used 100 mg of PLGA in DCM and mixed with 7 mg of protein (O/W1) and sonicated for 30 secs at 38% amplitude and then added the mixture in 0.5% PVA and sonicated for 30 secs at 38% amplitude. Then this whole mixture is mixed with 100 ml of 0.3% PVA again and stirred for 3 hrs to remove DCM.