Spreadsheet: 3a67e5f4-655f-486f-8db0-45bc73adaa13
Sheet: Sheet1

Table S2.  Point mutations included in the study. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12
a DDG values predicted by FoldX
New-function mutations
protein | mutation | DDG | ASA | DSSP | reference |  | protein | mutation | DDG | ASA | DSSP | reference
[NAME] | E227G | 2.15 | 0.3 | loop | [1] |  | DHFR | A26T | -0.31 | 0.21 | helix | [2]
[NAME] | F598S | -0.88 | 0.66 | loop | [1] |  | DHFR | H45R | -1.3 | 0.71 | helix | [2]
[NAME] | [NAME] | -1.3 | 0 | sheet | [1] |  | DHFR | I94L | 0.46 | 0.08 | loop | [2]
[NAME] | N503I | 0.62 | 0.51 | loop | [1] |  | DHFR | [NAME] | 0.32 | 0.18 | sheet | [2]
[NAME] | R20C | 2.79 | 0.23 | helix | [1] |  | DHFR | M20V | 1.29 | 0.2 | loop | [2]
[NAME] | V389L | -0.22 | 0 | loop | [1] |  | DHFR | [NAME] | 0.05 | 0.38 | helix | [2]
AspATs | A11T | -0.52 | 0.84 | loop | [3] |  | DHFR | W30R | -0.98 | 0.05 | helix | [2]
AspATs | A12T | 0.32 | 0.78 | loop | [4] |  | DHFR | F153S | 4.64 | 0.03 | sheet | [2]
AspATs | A293V | -0.11 | 0.84 | helix | [4] |  | DHFR | P21L | 2.11 | 0.58 | loop | [2]
AspATs | [NAME] | 0.49 | 0.16 | helix | [5] |  | DHFR | P21Q | 1.28 | 0.58 | loop | [2]
AspATs | G261A | -0.85 | 0.18 | loop | [4] |  | DHFR | I115V | 0.76 | 0.02 | sheet | [2]
AspATs | I37M | 0.87 | 0.19 | loop | [3] |  | DHFR | R158Q | 5.08 | 0.29 | sheet | [2]
AspATs | I353T | 2.95 | 0 | helix | [5] |  | DHFR | R158W | 5.54 | 0.29 | sheet | [2]
AspATs | K41Y | 0.29 | 0.24 | loop | [4] |  | DHFR | R159G | 3.6 | 0.91 | loop | [2]
AspATs | N142T | 1 | 0.43 | loop | [3] |  | DLH |  | -0.4 | 0.09 | loop | [6]
AspATs | N297S | 1.39 | 0.23 | loop | [3] |  | DLH | C123S | 1.8 | 0 | helix | [6]
AspATs | N34D | -0.86 | 0.14 | sheet | [3] |  | DLH | E36D | -0.21 | 0.01 | loop | [6]
AspATs | N69L | 1 | 0.8 | loop | [4] |  | DLH | R105H | 0.37 | 0.48 | helix | [6]
AspATs | P13T | 0.82 | 0.84 | loop | [4] |  | DLH | S208G | 0.11 | 0.38 | loop | [6]
AspATs | R129G | 4.06 | 0.43 | sheet | [4] |  | DoxG | I154F | -0.24 | 0.09 | sheet | [7]
AspATs | S139G | 2.75 | 0.16 | loop | [3] |  | DoxG | I154W | 0.21 | 0.09 | sheet | [7]
AspATs | S361S | 1.3 | 0 | sheet | [5] |  | DoxG | L190M | 0.37 | 0.19 | sheet | [7]
AspATs | S363G | 1.91 | 0.02 | sheet | [5] |  | DoxG | L190Y | 1.17 | 0.19 | sheet | [7]
AspATs | S285G | 1.54 | 0.5 | helix | [4] |  | DoxG | L242G | -0.16 | 0.01 | sheet | [7]
AspATs | T109S | 0.08 | 0.3 | helix | [4] |  | DoxG | L242S | 3.02 | 0.01 | sheet | [7]
AspATs | T47I | -0.77 | 0.37 | loop | [4] |  | DoxG | L242T | 5.06 | 0.01 | sheet | [7]
AspATs | V387L | 1.13 | 0.02 | sheet | [3] |  | DoxG | L242V | 2.63 | 0.01 | sheet | [7]
AspATs | V146I | -0.82 | 0.06 | helix | [4] |  | DoxG | P267L | -0.52 | 0.01 | sheet | [7]
AspATs | V39L | 0 | 0.27 | loop | [4] |  | DoxG | S191W | -2.88 | 0.02 | sheet | [7]
AspATs | R81H | 0.59 | 0.55 | helix | [4] |  | DoxG | S191Y | 0.7 | 0.02 | sheet | [7]
CAII | A65V | -0.08 | 0.03 | loop | [8] |  | EstB | A373T | 0.75 | 0 | sheet | [9]
CAII | T199A | 1.08 | 0.19 | loop | [8] |  | EstB | E316V | 0.39 | 0.62 | loop | [9]
CCP | D217G | 2.1 | 1 | loop | [10] |  | EstB | G349C | -0.78 | 0.15 | sheet | [9]
CCP | D224Y | -1.09 | 0.3 | sheet | [10] |  | EstB | H253L | 2.19 | 0.45 | loop | [9]
CCP | D217P | 1.94 | 1 | loop | [11] |  | EstB | I152T | 0.99 | 0.06 | loop | [9]
CCP | D224N | -1.09 | 0.3 | sheet | [11] |  | EstB | L135H | 2.18 | 0.1 | helix | [9]
CCP | R48H | 2.86 | 0.3 | helix | [11] |  | EstB | [NAME] | 1.26 | 0.14 | loop | [9]
CCP | T180A | 0.78 | 0.06 | loop | [11] |  | EstB | V351C | 1.13 | 0.14 | loop | [9]
New-function mutations
protein | mutation | DDG | ASA | DSSP | reference |  | protein | mutation | DDG | ASA | DSSP | reference
EstB | V351S | 2.2 | 0.14 | loop | [9] |  | Lipase | E160A | 1.09 | 0.52 | helix | [12]
GlmS | G471S | -0.58 | 0 | sheet | [13] |  | Lipase | I243S | 0.95 | 0.34 | loop | [12]
GlmS | L468P | 3.73 | 0.03 | sheet | [13] |  | Lipase | [NAME] | 2.76 | 0.08 | helix | [12]
GlmS | S449P | 0.79 | 0.76 | helix | [13] |  | Lipase | N163F | 4.42 | 0.01 | helix | [12]
hGST | I32V | 1.16 | 0.57 | sheet | [14] |  | Lipase | S155F | -1.16 | 0.63 | helix | [12]
hGST | I32M | -0.1 | 0.57 | sheet | [14] |  | Lipase | S155M | -1.81 | 0.63 | helix | [12]
hGST | I32S | 1.46 | 0.57 | sheet | [14] |  | Lipase | S161P | 1.63 | 0.52 | helix | [12]
hGST | I32L | 0.4 | 0.57 | sheet | [14] |  | Lipase | S164G | 0.42 | 0.38 | helix | [12]
hGST | H176Q | 3.95 | 0.04 | helix | [14] |  | Lipase | S199G | 1.07 | 0 | loop | [12]
hGST | W234R | 4.31 | 0.14 | helix | [14] |  | Lipase | S53P | 0.31 | 0 | helix | [12]
hGST | W234T | 4.09 | 0.14 | helix | [14] |  | Lipase | T180C | -0.75 | 1 | loop | [12]
hGST | W234P | 1.34 | 0.14 | helix | [14] |  | Lipase | T180I | 0.45 | 1 | loop | [12]
KDPG aldolase | G183R | 0.9 | 0.03 | sheet | [15] |  | Lipase | V272A | 1.3 | 0.03 | helix | [12]
KDPG aldolase | [NAME] | 3.43 | 0 | sheet | [15] |  | Lipase | [NAME] | 1.23 | 0.36 | helix | [12]
KDPG aldolase | K202M | 0.11 | 0.68 | helix | [15] |  | Lipase | F259L | 1.17 | 0.59 | loop | [16]
KDPG aldolase | K202N | -0.45 | 0.68 | helix | [15] |  | Lipase | [NAME] | -1.52 | 0.32 | loop | [16]
KDPG aldolase | [NAME] | 2.64 | 0.18 | helix | [15] |  | Lipase | K223Q | 0.39 | 0.65 | loop | [16]
KDPG aldolase | P77S | 1.01 | 0.36 | helix | [15] |  | Lipase | L17F | 1.69 | 0.26 | loop | [16]
KDPG aldolase | Q89R | -1.72 | 0.39 | loop | [15] |  | Lipase | [NAME] | 0.89 | 0 | loop | [16]
KDPG aldolase | R170S | 1.83 | 0.48 | helix | [15] |  | Lipase | N21D | 2.23 | 0.35 | sheet | [16]
KDPG aldolase | V22L | -0.24 | 0.29 | loop | [15] |  | Lipase | S158T | 0.55 | 0 | helix | [16]
Lipase | A34T | 0.11 | 0.15 | helix | [17] |  | Lipase | S161D | -0.04 | 0.52 | helix | [16]
Lipase | L208H | -0.25 | 0.62 | loop | [17] |  | Lipase | S284C | 0.46 | 0.72 | loop | [16]
Lipase | M16L | 0.93 | 0.27 | loop | [17] |  | Lipase | Y8H | 4.75 | 0.11 | loop | [16]
Lipase | P86L | 1.66 | 0.04 | helix | [17] |  | Lipase | A213D | 0.73 | 0.7 | helix | [18]
Lipase | Q275L | 0.47 | 0.53 | helix | [17] |  | Lipase | [NAME] | 0.34 | 0.95 | loop | [18]
Lipase | S237T | 0.16 | 0.01 | loop | [17] |  | Lipase | F265L | 0.22 | 0.84 | loop | [18]
Lipase | S268C | 0.83 | 0.46 | helix | [17] |  | Lipase | P96H | 1.69 | 0.23 | loop | [18]
Lipase | [NAME] | 2.4 | 0.38 | helix | [17] |  | OmpT protease | D208G | 3.68 | 0.01 | sheet | [19]
Lipase | T87S | 1.39 | 0 | helix | [17] |  | OmpT protease | S223R | -5.25 | 0.11 | sheet | [19]
Lipase | V232I | -0.33 | 0.02 | loop | [17] |  | OPDA | D208G | 4.24 | 0.05 | helix | [20]
Lipase | V55A | 1.11 | 0.38 | helix | [17] |  | OPDA | G348C | -0.54 | 0.27 | helix | [20]
Lipase | V94A | 1.34 | 0.39 | helix | [17] |  | OPDA | H254R | -5.6 | 0.08 | loop | [20]
Lipase | S149G | 0.44 | 0.59 | loop | [21], [12] |  | OPDA | I274T | 0.26 | 0.68 | loop | [20]
Lipase | S155L | -2.11 | 0.63 | helix | [21], [12] |  | OPDA | K185R | -1.89 | 0.27 | helix | [20]
Lipase | V47G | 3.49 | 0.03 | loop | [21], [12] |  | OPDA | K285R | -0.86 | 0.24 | helix | [20]
Lipase | N256L | -0.03 | 0.11 | sheet | [21] |  | OPDA | K294N | -0.52 | 0.46 | loop | [20]
Lipase | T234S | 1.24 | 0.05 | loop | [12], [12] |  | OPDA | N265D | -1.65 | 0.34 | loop | [20]
Lipase | D20N | 0.04 | 0.33 | loop | [12] |  | OPDA | [NAME] | -0.4 | 0.29 | helix | [20]
New-function mutations
protein | mutation | DDG | ASA | DSSP | reference |  | protein | mutation | DDG | ASA | DSSP | reference
OPDA | T352A | -0.56 | 0.33 | helix | [20] |  | PON | Y293H | 1.39 | 0.42 | loop | [22]
P450 2A6 | I140M | -0.16 | 0.12 | loop | [23] |  | PTE | F306C | 2.39 | 0.17 | loop | [24]
P450 2A6 | I300V | 0.88 | 0.05 | helix | [23] |  | PTE | H254R | -1.96 | 0.06 | loop | [24]
P450 2A6 | I366V | 0.52 | 0.17 | loop | [23] |  | PTE | P342A | 1.55 | 0.5 | loop | [24]
P450 2A6 | L204C | 0.2 | 0.15 | helix | [23] |  | PTE | A203T | -1.23 | 0.16 | loop | [25]
P450 2A6 | N297Q | 0.07 | 0.07 | helix | [23] |  | PTE | A80V | -0.16 | 0.04 | helix | [25]
PHP | K23Y | 0.08 | 0.43 | loop | [24] |  | PTE | K185R | -2.26 | 0.29 | helix | [25]
PHP | T210A | 6.4 | 0.01 | loop | [24] |  | PTE | P342S | 0.15 | 0.47 | loop | [25]
PHP | T245A | 1.22 | 0.21 | loop | [24] |  | PTE | V116I | 0.8 | 0 | helix | [25]
PON | F292L | 1.15 | 0.34 | helix | [26] |  | DAOCS | A106T | 0.06 | 0.43 | loop | [27]
PON | F292S | 3.02 | 0.34 | helix | [26] |  | DAOCS | C155Y | 2.24 | 0.06 | loop | [27]
PON | F292V | 1.68 | 0.34 | helix | [26] |  | DAOCS | C281R | -0.29 | 0.26 | helix | [27]
PON | [NAME] | 0.17 | 0.15 | helix | [26] |  | DAOCS | C281Y | 1.83 | 0.26 | helix | [27]
PON | K84Q | 0.55 | 0.27 | sheet | [26] |  | DAOCS | D107G | 0.65 | 0.9 | loop | [27]
PON | L69V | 0.67 | 0.09 | loop | [26] |  | DAOCS | G300V | 0.36 | 1 | loop | [27]
PON | N287D | -0.22 | 0.05 | loop | [26] |  | DAOCS | G79E | -0.49 | 0.58 | loop | [27]
PON | S138L | 2.09 | 0.31 | loop | [26] |  | DAOCS | H244Q | 1.33 | 0.04 | sheet | [27]
PON | S193P | 4.35 | 0.52 | helix | [26] |  | DAOCS | I305L | -0.53 | 0.44 | sheet | [27]
PON | T332A | 1.1 | 0 | sheet | [26] |  | DAOCS | I305M | -0.41 | 0.44 | sheet | [27]
PON | V346A | 1.12 | 0.16 | loop | [26] |  | DAOCS | L277Q | -0.34 | 0.35 | helix | [27]
PON | V346M | -0.45 | 0.16 | loop | [26] |  | DAOCS | M188I | 0.67 | 0.01 | loop | [27]
PON | Y293D | 2.55 | 0.42 | loop | [26] |  | DAOCS | M188V | -0.16 | 0.01 | loop | [27]
PON | A180P | 1.58 | 0 | sheet | [22] |  | DAOCS | M73T | 2.12 | 0.1 | loop | [27]
PON | A187I | -0.97 | 0.37 | loop | [22] |  | DAOCS | N304K | 1.41 | 0.17 | loop | [27]
PON | E354G | 1.82 | 0.39 | sheet | [22] |  | DAOCS | V275I | -0.1 | 0.04 | helix | [27]
PON | I291F | -0.27 | 0.15 | helix | [22] |  | DAOCS | [NAME] | 1.92 | 0.28 | sheet | [27]
PON | I291T | 1.11 | 0.15 | helix | [22] |  | TBPA | D104G | 2.14 | 0.13 | loop | [28]
PON | I291V | 0.15 | 0.15 | helix | [22] |  | TBPA | H26Y | -0.29 | 0.29 | loop | [28]
PON | K192A | 1.29 | 0.16 | helix | [22] |  | TBPA | S106G | 0.7 | 0.06 | loop | [28]
PON | K192G | 2.64 | 0.16 | helix | [22] |  | TBPA | V121A | 1.47 | 0.03 | helix | [28]
PON | L240M | 0.18 | 0.02 | loop | [22] |  | TEM1 | A237T | -0.27 | 0.38 | sheet | [31]
PON | L240T | 0.82 | 0.02 | loop | [22] |  | TEM1 | A42G | 1.28 | 0.17 | loop | [31]
PON | L355R | 0.64 | 0.56 | loop | [22] |  | TEM1 | D179G | 2.88 | 0.02 | loop | [31]
PON | N43D | 0.69 | 0.65 | sheet | [22] |  | TEM1 | D179N | 2.38 | 0.02 | loop | [31]
PON | P286S | 3.54 | 0.04 | loop | [22] |  | TEM1 | E104K | -0.76 | 0.8 | loop | [31]
PON | P59L | 1.24 | 0.8 | loop | [22] |  | TEM1 | E240K | 0.4 | 0.72 | loop | [31]
PON | S166G | 2.76 | 0.03 | loop | [22] |  | TEM1 | G238A | -0.3 | 0.34 | loop | [31]
PON | T332S | 0.49 | 0 | sheet | [22] |  | TEM1 | G238S | -0.48 | 0.34 | loop | [31]
PON | Y190I | 0.64 | 0.48 | helix | [22] |  | TEM1 | M69I | 1.38 | 0.03 | helix | [31]
New-function mutations
protein | mutation | DDG | ASA | DSSP | reference
TEM1 | M69L | 0.21 | 0.03 | helix | [31]
TEM1 | M69V | 1.59 | 0.03 | helix | [31]
TEM1 | N276D | 0.98 | 0.11 | helix | [31]
TEM1 | Q39K | 0.26 | 0.72 | helix | [31]
TEM1 | R164C | 2.91 | 0.14 | loop | [31]
TEM1 | R164H | 4.03 | 0.14 | loop | [31]
TEM1 | R164S | 4.9 | 0.14 | loop | [31]
TEM1 | R275L | 0.65 | 0.11 | helix | [31]
TEM1 | R275Q | 0.83 | 0.11 | helix | [31]
VAO | A429V | -2.92 | 0 | sheet | [29]
VAO | E502G | 1.44 | 0.01 | loop | [29]
VAO | I238T | 2.94 | 0.01 | loop | [29]
VAO | P454Y | -0.81 | 0.02 | loop | [29]
VAO | T505S | -0.82 | 0.05 | loop | [29]
Other mutations
protein | mutation | DDG | ASA | DSSP | reference |  | protein | mutation | DDG | ASA | DSSP | reference
[NAME] | D506N | 0.51 | 0.47 | loop | [1] |  | AspATs | A93T | 0.26 | 0.53 | helix | [4]
[NAME] | E62K | 0.25 | 0.21 | helix | [1] |  | AspATs | D169N | 1.01 | 0.41 | loop | [4]
[NAME] | N76D | -2.12 | 0.67 | loop | [1] |  | AspATs | D325N | -0.36 | 0.57 | helix | [4]
[NAME] | Q613H | 0.94 | 0.63 | loop | [1] |  | AspATs | D97N | -0.21 | 0.64 | helix | [4]
AspATs | A253V | -1.51 | 0 | sheet | [3] |  | AspATs | E214K | 0.13 | 0.84 | helix | [4]
AspATs | A269T | 0.86 | 0 | sheet | [3] |  | AspATs | E402K | -0.4 | 0.62 | helix | [4]
AspATs | A290V | -0.51 | 0.1 | helix | [3] |  | AspATs | E43G | 1.88 | 0.56 | loop | [4]
AspATs | A293V | -0.56 | 0.84 | loop | [3] |  | AspATs | E7D | 0.39 | 1 | loop | [4]
AspATs | A381V | -2.74 | 0.05 | loop | [3] |  | AspATs | F223S | 3.31 | 0.01 | sheet | [4]
AspATs | E234D | 1.25 | 0.54 | helix | [3] |  | AspATs | F362L | 0.1 | 0.12 | sheet | [4]
AspATs | E278K | -1.8 | 0.55 | helix | [3] |  | AspATs | G151A | 0.82 | 0.58 | loop | [4]
AspATs | E7V | 1.03 | 1 | loop | [3] |  | AspATs | G216D | 0.45 | 0.41 | loop | [4]
AspATs | G197S | 2.09 | 0 | loop | [3] |  | AspATs | G31D | -0.23 | 0.76 | loop | [4]
AspATs | G72S | -0.85 | 0.57 | loop | [3] |  | AspATs | G364S | 1.04 | 0.58 | loop | [4]
AspATs | H143Y | 0.21 | 0 | sheet | [3] |  | AspATs | G72D | -0.85 | 0.56 | loop | [4]
AspATs | K126G | 1.05 | 0.57 | loop | [3] |  | AspATs | G80S | 0.11 | 0.13 | helix | [4]
AspATs | [NAME] | -2.08 | 0.57 | loop | [3] |  | AspATs | G89S | 1.93 | 0.1 | loop | [4]
AspATs | K41N | 0.79 | 0.3 | loop | [3] |  | AspATs | I309V | 0.5 | 0 | helix | [4]
AspATs | M326K | 0.89 | 0.05 | helix | [3] |  | AspATs | I318T | 0.62 | 0.31 | helix | [4]
AspATs | M397L | -0.13 | 0.02 | helix | [3] |  | AspATs | I318V | 0.58 | 0.31 | helix | [4]
AspATs | N142I | 0.07 | 0.43 | loop | [3] |  | AspATs | I73T | 1.1 | 0.15 | loop | [4]
AspATs | N339D | -1.44 | 0.38 | helix | [3] |  | AspATs | I76V | 0.35 | 0.18 | loop | [4]
AspATs | [NAME] | 0.58 | 0.01 | sheet | [3] |  | AspATs | K121E | 1.53 | 0.79 | helix | [4]
AspATs | Q226L | 0.66 | 0.02 | loop | [3] |  | AspATs | [NAME] | -2.08 | 0.66 | loop | [4]
AspATs | R282C | 2.08 | 0.56 | helix | [3] |  | AspATs | [NAME] | 1.15 | 0.66 | loop | [4]
AspATs | R348C | -3.41 | 0.35 | loop | [3] |  | AspATs | K98R | -1.05 | 0.43 | loop | [4]
AspATs | S311G | 0.47 | 0.6 | helix | [3] |  | AspATs | N137D | 1.34 | 0.56 | loop | [4]
AspATs | T308A | 1.14 | 0.17 | helix | [3] |  | AspATs | N175S | 1.47 | 0.79 | helix | [4]
AspATs | A242T | -0.22 | 0.15 | helix | [4] |  | AspATs | N178Y | 1.84 | 0.53 | helix | [4]
AspATs | R282C | 1.64 | 0.62 | helix | [4] |  | AspATs | N312D | -1.86 | 0.5 | loop | [4]
AspATs | A14V | 0.43 | 0.88 | loop | [4] |  | AspATs | N339S | -0.17 | 0.44 | helix | [4]
AspATs | A163V | 0.46 | 0.61 | loop | [4] |  | AspATs | N396D | 0.39 | 0.21 | helix | [4]
AspATs | A21V | -0.02 | 0.33 | helix | [4] |  | AspATs | N63S | 0.7 | 0.63 | helix | [4]
AspATs | A242T | -0.33 | 0.15 | helix | [4] |  | AspATs | N69S | 0.05 | 0.8 | loop | [4]
AspATs | A26V | 0.6 | 0.74 | loop | [4] |  | AspATs | N96D | 0.89 | 0.76 | helix | [4]
AspATs | A314T | 0.34 | 0.57 | helix | [4] |  | AspATs | N96S | -0.01 | 0.76 | helix | [4]
AspATs | A317V | 0.34 | 0.46 | helix | [4] |  | AspATs | Q206R | 0.98 | 0.49 | loop | [4]
AspATs | A381V | -1.57 | 0.05 | loop | [4] |  | AspATs | Q342R | -0.23 | 0.57 | helix | [4]
AspATs | A398V | 0.44 | 0.21 | helix | [4] |  | AspATs | Q369H | -0.26 | 0.2 | helix | [4]
Other mutations
protein | mutation | DDG | ASA | DSSP | reference |  | protein | mutation | DDG | ASA | DSSP | reference
AspATs | R332H | 1.23 | 0.66 | helix | [4] |  | DHFR | D116Y | 0.34 | 0.59 | loop | [2]
AspATs | R348H | -1.33 | 0.14 | loop | [4] |  | DHFR | E154K | -1.09 | 0.23 | sheet | [2]
AspATs | R81S | 0.97 | 0.55 | helix | [4] |  | DHFR | P129L | 0.35 | 0.58 | loop | [2]
AspATs | S277C | 0.04 | 0.47 | helix | [4] |  | DHFR | Q65R | -0.68 | 0.66 | loop | [2]
AspATs | T167A | 0.04 | 0.43 | sheet | [4] |  | DHFR | S3R | 3.58 | 0 | sheet | [2]
AspATs | T340M | -0.41 | 0.14 | helix | [4] |  | DHFR | W74C | 2.69 | 0.2 | sheet | [2]
AspATs | T44A | 2.32 | 0.71 | loop | [4] |  | DLH | 173 | 1.79 | 0.39 | loop | [6]
AspATs | [NAME] | 0.72 | 0.59 | helix | [4] |  | DLH | A134S | -0.14 | 0.09 | helix | [6]
AspATs | V280A | 1.84 | 0 | helix | [4] |  | DLH | A229V | 0.11 | 0.41 | helix | [6]
AspATs | V379I | -0.6 | 0.03 | sheet | [4] |  | DLH | G211D | -1.14 | 0.3 | loop | [6]
AspATs | E154V | 1.35 | 0.46 | sheet | [4] |  | DLH | R45Q | 1.06 | 0.48 | helix | [6]
CAII | D110N | 0.12 | 0.35 | loop | [8] |  | DLH | R81A | 2.53 | 0.34 | helix | [6]
CCP | E93G | 1.75 | 0.46 | helix | [10] |  | DoxG | L182S | 0.03 | 0.26 | loop | [7]
CCP | G88S | -0.9 | 0 | helix | [10] |  | DoxG | H59L | 0.82 | 0.24 | loop | [7]
CCP | [NAME] | -2.43 | 0.74 | loop | [10] |  | DoxG | I265L | -0.74 | 0.02 | sheet | [7]
CCP | N184Y | -0.93 | 0.33 | helix | [10] |  | DoxG | K165R | 0.93 | 0.46 | helix | [7]
CCP | Q292L | 2.47 | 0.32 | helix | [10] |  | DoxG | K300I | 2.51 | 0.82 | loop | [7]
CCP | Y39H | -0.74 | 0.56 | helix | [10] |  | DoxG | L253M | 1.7 | 0.19 | sheet | [7]
CCP | A194T | -0.18 | 0.32 | loop | [11] |  | DoxG | D21E | -0.11 | 0.8 | helix | [7]
CCP | A25V | -0.99 | 0.15 | helix | [11] |  | DoxG | V290A | -0.94 | 0.34 | loop | [7]
CCP | D148N | -0.03 | 0.57 | loop | [11] |  | DoxG | Q101R | -3.01 | 0.78 | helix | [7]
CCP | D279G | 0.34 | 1 | loop | [11] |  | DoxG | S191T | -0.57 | 0.02 | sheet | [7]
CCP | E201G | 4.67 | 0.13 | helix | [11] |  | EstB | I245V | 0.85 | 0.3 | sheet | [9]
CCP | E35G | 1.7 | 0.76 | helix | [11] |  | EstB | R308C | 0.03 | 0.59 | loop | [9]
CCP | K226R | -0.9 | 0.79 | loop | [11] |  | GlmS | A38T | -0.1 | 0.73 | loop | [13]
CCP | M119V | 1.74 | 0.07 | helix | [11] |  | GlmS | D386V | -0.34 | 0.7 | loop | [13]
CCP | N164D | -2.64 | 0.57 | helix | [11] |  | GlmS | [NAME] | 1.56 | 0.57 | helix | [13]
CCP | N184D | 0.66 | 0.33 | helix | [11] |  | GlmS | E524G | 0.24 | 0.86 | loop | [13]
CCP | N87D | -0.63 | 0.43 | helix | [11] |  | GlmS | I271T | 1.74 | 0.16 | sheet | [13]
CCP | P190L | 2.29 | 0.19 | loop | [11] |  | GlmS | I3T | 0.35 | 0 | sheet | [13]
CCP | P94A | 2.44 | 0.42 | helix | [11] |  | GlmS | R249C | 0.48 | 0.71 | loop | [13]
CCP | S246G | 0.7 | 0.7 | helix | [11] |  | hGST | L9P | 0.9 | 0.18 | loop | [14]
CCP | S282G | 1.15 | 0.81 | loop | [11] |  | hGST | C14S | 1.68 | 0 | helix | [14]
CCP | T288G | 1.94 | 0.1 | helix | [11] |  | hGST | K23M | 1.24 | 0.38 | helix | [14]
CCP | V154I | -0.84 | 0.03 | helix | [11] |  | hGST | D25G | -0.38 | 0.98 | loop | [14]
CCP | V5I | -0.76 | 0.63 | loop | [11] |  | hGST | I26V | 1.22 | 0.04 | loop | [14]
CCP | V7I | -0.84 | 0.46 | sheet | [11] |  | hGST | R31H | 0.76 | 0.37 | sheet | [14]
DHFR | A144T | 0.38 | 0.62 | loop | [2] |  | hGST | I36F | 0.26 | 0.14 | helix | [14]
DHFR | A84V | -0.04 | 0.25 | helix | [2] |  | hGST | I36N | 2.74 | 0.14 | helix | [14]
Other mutations
protein | mutation | DDG | ASA | DSSP | reference |  | protein | mutation | DDG | ASA | DSSP | reference
hGST | Q47L | 1.1 | 0.9 | helix | [14] |  | Lipase | T226A | 0.54 | 0.37 | loop | [17]
hGST | V54A | 0.8 | 0.11 | loop | [14] |  | OmpT protease | D214V | 0.52 | 0.45 | loop | [19]
hGST | K58R | -0.2 | 0.37 | sheet | [14] |  | OmpT protease | S17G | 1.57 | 0.03 | sheet | [19]
hGST | L89P | -1.19 | 0.33 | helix | [14] |  | PHP | [NAME] | 0.87 | 0.69 | sheet | [24]
hGST | A91T | -0.2 | 0.11 | helix | [14] |  | PHP | T183I | -0.02 | 0 | loop | [24]
hGST | L111V | 1.81 | 0.09 | helix | [14] |  | PON | E249K | -0.78 | 0.53 | sheet | [26]
hGST | A113V | 0.56 | 0 | helix | [14] |  | PON | F17Y | -0.73 | 0.79 | loop | [26]
hGST | M119L | 0.55 | 0 | helix | [14] |  | PON | G339E | 0.95 | 0.55 | loop | [26]
hGST | Q131R | 0.06 | 0.95 | helix | [14] |  | PON | H31Y | 1.04 | 0.9 | loop | [26]
hGST | A138T | 0.09 | 0.5 | helix | [14] |  | PON | I109M | 0.51 | 0.53 | loop | [26]
hGST | V142A | -0.36 | 0.5 | helix | [14] |  | PON | I343M | 1.24 | 0 | sheet | [26]
hGST | T143S | 0.86 | 0.01 | helix | [14] |  | PON | I65L | 0.91 | 0.01 | sheet | [26]
hGST | T143I | -0.83 | 0.01 | helix | [14] |  | PON | K145R | -0.2 | 0.31 | sheet | [26]
hGST | L147F | 1.22 | 0 | helix | [14] |  | PON | [NAME] | 1.08 | 1 | loop | [26]
hGST | T172A | 2.82 | 0 | helix | [14] |  | PON | S22P | -0.88 | 0.49 | helix | [26]
hGST | M175L | -0.46 | 0.01 | helix | [14] |  | PON | V213A | 1.92 | 0.2 | sheet | [26]
hGST | G179S | 0.75 | 0.05 | helix | [14] |  | PON | V30A | -0.6 | 0.17 | loop | [26]
hGST | [NAME] | 0.39 | 0.4 | helix | [14] |  | PON | V97A | 1.95 | 0.2 | loop | [26]
hGST | A192T | -0.64 | 0.3 | helix | [14] |  | PON | D122G | -0.24 | 0.21 | sheet | [22]
hGST | T193A | 3.1 | 0.52 | helix | [14] |  | PON | D49G | 0.13 | 0.68 | loop | [22]
hGST | A210V | -0.21 | 0 | helix | [14] |  | PON | E323G | 5.71 | 0.09 | sheet | [22]
hGST | V213A | 0.74 | 0.23 | helix | [14] |  | PON | F220L | 0.83 | 0.01 | sheet | [22]
hGST | K216R | 0.46 | 0.53 | helix | [14] |  | PON | F264L | 2.16 | 0.12 | loop | [22]
hGST | K218E | 0.75 | 0.56 | helix | [14] |  | PON | F98L | 1.31 | 0.38 | loop | [22]
hGST | P221S | 1.4 | 0.73 | loop | [14] |  | PON | I271T | 2.81 | 0.03 | sheet | [22]
hGST | P222L | 1.02 | 0.67 | loop | [14] |  | PON | K162E | -0.94 | 0.86 | loop | [22]
hGST | P225L | 2.08 | 0.69 | helix | [14] |  | PON | K250R | -2.81 | 0.21 | sheet | [22]
hGST | L236P | 1.7 | 0.49 | helix | [14] |  | PON | K297R | -1.68 | 0.84 | loop | [22]
hGST | A237G | 0.75 | 0.2 | helix | [14] |  | PON | S272F | -3.76 | 0.15 | sheet | [22]
hGST | I239F | 0.66 | 0.05 | helix | [14] |  | PON | S82P | 0.09 | 0.49 | loop | [22]
hGST | R240C | 0.82 | 0.43 | loop | [14] |  | PON | T103A | 0.39 | 0.5 | sheet | [22]
Lipase | A102V | -1.29 | 0 | sheet | [17] |  | PON | T257A | 0.68 | 0.43 | sheet | [22]
Lipase | D113G | 0.45 | 0.44 | helix | [17] |  | PON | T35A | 0.57 | 0.78 | loop | [22]
Lipase | I142T | 1.01 | 0.18 | helix | [17] |  | PON | Y190H | -0.14 | 0.91 | helix | [22]
Lipase | S112P | 2.83 | 0.09 | helix | [17] |  | PON | Y208N | 3.03 | 0.14 | sheet | [22]
Lipase | S147N | -0.21 | 0.65 | helix | [17] |  | PON | Y352H | 0.34 | 0.11 | sheet | [22]
Lipase | S218L | -1.04 | 0.15 | helix | [17] |  | PON | A296P | -1.48 | 0.73 | loop | [30]
Lipase | S268N | -0.22 | 0.46 | helix | [17] |  | PON | D123E | -0.21 | 1 | loop | [30]
Lipase | T150A | -0.83 | 0.63 | loop | [17] |  | PON | D354E | -0.53 | 1 | sheet | [30]
Other mutations
protein | mutation | DDG | ASA | DSSP | reference |  | protein | mutation | DDG | ASA | DSSP | reference
PON | E101G | 1.42 | 0.76 | sheet | [30] |  | TEM1 | D163G | 0.93 | 0.31 | loop | [31]
PON | E297K | -0.42 | 0.94 | loop | [30] |  | TEM1 | D35P | 0.56 | 0.46 | helix | [31]
PON | E94D | -0.03 | 0.77 | loop | [30] |  | TEM1 | G196D | -0.46 | 0.3 | loop | [31]
PON | G136N | 1.38 | 0.88 | loop | [30] |  | TEM1 | G196S | 1.29 | 0.3 | loop | [31]
PON | I103T | 0.16 | 0.35 | sheet | [30] |  | TEM1 | G218E | -1.84 | 0.53 | loop | [31]
PON | I121T | 1.76 | 0.35 | sheet | [30] |  | TEM1 | G92D | -0.78 | 0.76 | loop | [31]
PON | I187A | 0.99 | 0.32 | loop | [30] |  | TEM1 | H153R | -5.19 | 0.56 | helix | [31]
PON | I48V | 1.7 | 0.01 | loop | [30] |  | TEM1 | H158N | -0.07 | 0.74 | loop | [31]
PON | [NAME] | -0.7 | 0.95 | loop | [30] |  | TEM1 | H289L | -1.07 | 0.3 | loop | [31]
PON | [NAME] | 3.24 | 0.39 | loop | [30] |  | TEM1 | I127V | 1.04 | 0 | helix | [31]
PON | R19G | 1.48 | 0.63 | helix | [30] |  | TEM1 | I173V | 0.22 | 0.34 | loop | [31]
PON | R260K | 2.51 | 0.29 | sheet | [30] |  | TEM1 | K34E | 1.13 | 0.53 | helix | [31]
PON | S263D | -1.87 | 0.71 | sheet | [30] |  | TEM1 | L221K | 1.31 | 0.04 | helix | [31]
PON | S98L | -1.64 | 0.71 | loop | [30] |  | TEM1 | [NAME] | 2.41 | 0.1 | loop | [31]
PTE | A176V | -0.74 | 0.05 | loop | [24] |  | TEM1 | M182T | -0.32 | 0 | loop | [31]
PTE | Q290R | -0.19 | 0.57 | helix | [24] |  | TEM1 | N175I | 1.23 | 1 | loop | [31]
PTE | T45A | 0.96 | 0.23 | sheet | [24] |  | TEM1 | P145R | 2.97 | 0.07 | helix | [31]
PTE | V320A | 1 | 0.31 | helix | [24] |  | TEM1 | R204Q | 2.31 | 0.19 | helix | [31]
PTE | V84A | 1.21 | 0.17 | helix | [24] |  | TEM1 | S124N | -0.18 | 0.32 | helix | [31]
PTE | H257Y | -1.42 | 0.03 | loop | [25] |  | TEM1 | S268G | 3.79 | 0.05 | loop | [31]
PTE | I274N | 0.01 | 0.63 | loop | [25] |  | TEM1 | T114P | -0.63 | 0.91 | loop | [31]
PTE | L87S | 3.3 | 0 | helix | [25] |  | TEM1 | T265M | -2.5 | 0.05 | sheet | [31]
PTE | Q148R | -2.67 | 0.68 | helix | [25] |  | TEM1 | V262I | -0.6 | 0 | sheet | [31]
PTE | R67H | 0.58 | 0.42 | helix | [25] |  | TEM1 | V80E | 2.66 | 0.01 | helix | [31]
DAOCS | A11V | 0.65 | 0.29 | helix | [27] |  | TEM1 | V84I | -0.05 | 0.18 | helix | [31]
DAOCS | A140V | -0.68 | 0.04 | helix | [27] |  | TEM1 | [NAME] | -0.79 | 0.37 | loop | [31]
DAOCS | A198T | -0.11 | 0.89 | loop | [27] |  | VAO | F93Y | -0.4 | 0.11 | helix | [29]
DAOCS | [NAME] | -0.3 | 0.52 | helix | [27] |  | VAO | P189S | 2.66 | 0.3 | loop | [29]
DAOCS | E144K | -0.25 | 0.82 | loop | [27] |  | VAO | M437L | 0.72 | 0.26 | helix | [29]
DAOCS | I118V | 0.23 | 0.08 | helix | [27]
DAOCS | P145L | 1.96 | 0.03 | loop | [27]
DAOCS | R135Q | 0.7 | 0 | helix | [27]
DAOCS | R179Q | -1.09 | 0.4 | loop | [27]
DAOCS | T259I | -0.57 | 0.05 | sheet | [27]
TBPA | P256L | 1.48 | 0.1 | loop | [28]
TEM1 | A184V | -0.72 | 0.2 | helix | [31]
TEM1 | A224K | -1.83 | 0.27 | helix | [31]
TEM1 | A280V | 0.16 | 0.13 | helix | [31]
TEM1 | D157E | 1.09 | 0.08 | loop | [31]
Reference
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