Spreadsheet: 17a62b23-db29-4faa-8328-7396469edb76
Sheet: Sheet1

Table S2: Relative gene expression in splenocytes derived from differentially vaccinated mice | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12 | Unnamed: 13 | Unnamed: 14 | Unnamed: 15 | Unnamed: 16 | Unnamed: 17 | Unnamed: 18 | Unnamed: 19 | Unnamed: 20 | Unnamed: 21
 |  |  |  |  |  |  |  | Experiment number#
 |  | 1 | 2a% | 2b | 3 | 4 |  |  |  | 5 |  |  |  | 6 |  |  |  | 7
[NAME] group: |  | LVS | LVS |  | LVS | LVS | LVS-G | LVS-R | HK-LVS | LVS | LVS-G | LVS-R | HK-LVS | LVS | LVS-G | LVS-R | HK-LVS | LVS | LVS-G | LVS-R | HK-LVS
Survival / total:* |  | N.A. | N.A. | N.A. | N.A. | 5/5 | 5/5 | 2/5 | 0/5 | 5/5 | 5/5 | 2/5 | 1/5 | 5/5 | 5/5 | 3/5 | 0/5 | 5/5 | 4/5 | 3/5 | 0/5
Gene | Detection‡
Bcl6 | Profiler | 1.32~ | 1.319507910772896 | 1.274560627319263 | 1.433955248015827 | 1.098854217576879 | 1.100378609366136 | 1.248330548901612 | 1.129747214570122 | 1.677136368881861 | 1.75807912182357 | 1.367935303902486 | 1.112650120584831 | 0.8573760366341687 | 1.011152080851303 | 1.033830736247963 | 1.156688183905285
Ccl11 | Profiler
[NAME] | Profiler | 1.274560627319263 | 1.591072967509839 | 1.892115293451194 | 0.7955364837549197 | 0.844400887423782 | 0.7412335049014183 | 0.655196701929182 | 0.7199656592573428 | 1.586667686382282 | 1.089752112213325 | 1.24142749152611 | 0.4678117489156989 | 1.19581979677612 | 1.16150873192572 | 1.033830736247963 | 0.806641759222125
Ccl7 | Profiler | 1.132883885295799 | 1.526259208960562 | 1.815038310634321 | 0.7422617853145246 | 1.835279764622732 | 1.876442393001343 | 0.8235910172675744 | 0.3994259582245745 | 2.762548896287709 | 1.758079121823574 | 1.224336392400203 | 0.66158571995406 | 1.599920256665502 | 1.10649735309227 | 1.255271991282643 | 0.8235910172675724
Ccl7 | qRT-PCR |  |  |  |  | 2.62 | 1.41 | 0.5 | 0.57 | 2.27 | 1.85 | 0.51 | 0.72 | 2.47 | 1.57 | 0.65 | 0.32 | 1.9 | 0.25 | 0.63 | 2.01
Ccr10 | Profiler |  |  |  |  | 0.6355158446528338 |  | 1.086734862526059 | 0.4340701138005205 |  | 0.195331970043079 | 0.5872309856068239 |  | 1.679462985888282 | 1.068805991211011 | 0.8224500687124648 | 0.4697613746070085
Ccr2 | Profiler | 3.095129987084777 | 0.9012504626108307 | 0.858565436437755 | 0.5285090202806895 | 0.4951714359665114 | 0.5169153681239828 | 0.7474246243174685 | 1.429984986361689 | 0.3112185588455423 | 0.5190696352715283 | 0.8538177143912444 | 0.7652483849113324 | 0.5617499515158207 | 0.3752697743785922 | 0.6680370391950491 | 1.20580782769076
Ccr2 | qRT-PCR |  |  |  |  | 0.34 | 0.43 | 0.24 | 1.58 | 0.34 | 0.45 | 1 | 1.47 | 0.31 | 0.66 | 0.32 | 0.64 | 8.15 | 2.96 | 2.84 | 4.38
Ccr3 | Profiler | 2.828427124746198 | 2.69446715373138 | 1.741101126592249 | 1.094293701260741 | 1.586667686382286 | 1.451958279903895 | 1 | 1.315854525029009 | 0.8741786204103716 | 0.7652483849113325 | 0.8304700240908919 | 0.6258982293269966 | 2.262628925691795 | 0.9240225724468204 | 1.800003859587022 | 1.840375301249748
Ccr3 | qRT-PCR |  |  |  |  | 0.33 | 0.67 | 0.2 | 1.64 | 0.2 | 0.2 | 0.5 | 0.58 | 0.3 | 0.3 | 0.1 | 0.41 | 10.93 | 0.52 | 0.79 | 6.67
Ccr4 | Profiler | 24.76103989667822 |  | 0.1051120519067142 | 0.04769560028001756 | 0.8681402276010449 | 0.5815600207739259 | 1.057018040561382 | 0.4556506403185719 | 1.480413298000052 | 1.488645255166542 | 0.5913155001220702 | 0.4710656369524051 | 0.8573760366341715 | 0.8802590135631503 | 1.431968741201145 | 1.827662900458803
Ccr5 | Profiler | 6.020986989644268 | 2.445280555384142 | 2.027918959580063 | 1.547564993542393 | 1.939923820683469 | 1.599920256665506 | 0.8766057213160341 | 0.7453551933994585 | 1.470187336389038 | 1.269270886019803 | 1.26751252203442 | 0.8609491883389011 | 2.964934019605665 | 1.631274986678014 | 3.090842198591942 | 1.635804117115559
Ccr5 | qRT-PCR |  |  |  |  | 1.95 | 2.1 | 0.38 | 0.52 | 1.47 | 1.28 | 1.4 | 1.71 | 2.03 | 2.56 | 1.6 | 1.2 | 30.64 | 5.32 | 4.62 | 6.04
CD27 | Profiler | 2 | 0.829319545814442 | 1.414213562373095 | 1.00695555005672 | 0.6717517128606478 | 0.7944344000961063 | 0.9726549474122861 | 1.22773568391506 | 0.8621435450175947 | 0.6479704827166647 | 0.896266699569402 | 0.689680460913528 | 0.7673730480004427 | 0.5609717402652409 | 0.6189951454187226 | 1.071773462536294
Cd28 | Profiler | 3.271608234231126 | 1.484523570629049 | 1.337927554786114 | 0.9460576467255972 | 0.605416541975277 | 0.5937798330367058 | 0.7737824967711966 | 0.8925469714729781 | 0.7100536792717139 | 0.7599624282007577 | 0.7641882603825821 | 1.269270886019802 | 0.7110387052936352 | 0.6054165419752757 | 0.7780851765554176 | 1.310393403858364
Cd4 | Profiler | 3.890619789649154 | 0.6877709090698743 | 1.375541818139744 | 1.375541818139748 | 0.6311260160560572 | 0.5735535121130828 | 0.7631296044802792 | 0.7716400875294663 | 0.690637224071929 | 0.652477473844479 | 0.8133791981321248 | 0.4710656369524037 | 1.534746096000886 | 0.6954409858549938 | 2.01112116079694 | 2.219138944135694
CD40 | Profiler | 3.010493494822135 | 0.7120250977985366 | 0.83508791942837 | 1.231144413344917 | 1.470187336389038 | 1.656341322669093 | 1.375541818139744 | 1.091263877054933 | 1.554014537568098 | 1.721898376677806 | 1.285206337469371 | 0.9821395952633915 | 0.9917167307382906 | 1.210833083950551 | 1.195819796776124 | 0.8888426811665692
CD40lg | Profiler | 3.386981249450102 | 0.901250462610831 | 0.9395227492140141 | 1.035264923841378 | 1.046810281974575 | 1.026689545720002 | 1.337927554786114 | 1.352848231050274 | 0.7824117817365099 | 0.3377435211339088 | 0.4358791197685311 | 0.9686181892266258 | 0.7061272021923781 | 0.5126336189442973 | 0.6588399758670698 | 1.021012125707194
Cd80 | Profiler | 4.141059695365513 | 1.028113826656071 | 1.319507910772897 | 0.8526348917679559 | 1.420107358543427 | 1.139183377222117 | 0.8645372313078664 | 0.6764241155251371 | 1.025267237888593 | 0.7290046894082879 | 0.4038803888048033 | 0.2167343908786711 | 0.9189128834790473 | 1.068805991211008 | 1.237990290837445 | 1.028113826656066
Cd86 | Profiler | 1.879045498428028 | 0.8010698775896218 | 0.9726549474122862 | 0.9201876506248768 | 1.860899315406027 | 1.800003859587023 | 1.25701337452183 | 0.936921446872425 | 1.797510253222059 | 1.65175532987144 | 1.31221125459196 | 0.3570009949212659 | 1.451958279903895 | 1.772763398389735 | 2.111107435275932 | 0.8950250709279745
Cebpb | Profiler | 4.112455306624274 | 1.484523570629056 | 1.802500925221662 | 1.19747870461893 | 1.362258034640486 | 1.212512819061736 | 0.9930924954370346 | 0.9176398823113635 | 1.169587664051946 | 1.009751297596685 | 1.18263100024414 | 1.045360100205162 | 1.969732885791099 | 1.665551541761099 | 1.8378257669581 | 1.140763715868425
Crebbp | Profiler | 0.4413514981453276 | 1.558329159321 | 0.9930924954370373 | 1.064370182453362 | 3.002158195028738 | 1.929196369168277 | 2.751083636279489 | 3.472560910404169 | 0.950000382874885 | 0.9421312739048074 | 0.3928362583337895 | 1.408344226912077 | 1.942614991083265 | 0.7149924931808442 | 2.617156142910016 | 2.445280555384135
Csf2 | Profiler | 11.39240156477655 | 0.7269862586601546 | 1.840375301249753 | 2.496661097803224 | 3.773753003581442 | 5.688309642349098 | 0.9265880618903715 | 0.4135116842216326 | 3.545526796779461 | 2.609909895377908 | 0.8479199646331437 | 0.2197598902279456 | 0.8055242910487473 | 5.680429434173695 | 3.382289149510064 | 0.8585654364377541
Csf2 | qRT-PCR |  |  |  |  | 10.18 | 11.54 | 0.47 | 0.28 | 1.58 | 1.51 | 0.44 | 0.32 | 0.43 | 3.75 | 1.18 | 0.31 | 6.06 | 3.85 | 2.26 | 1.71
Ctla4 | Profiler | 1.5691681957935 | 0.9526379980439394 | 1.265756593970283 | 0.697371833175203 | 0.9699619103417343 | 1.041021597684111 | 0.9794202975869283 | 1.025267237888592 | 1.185914499410947 | 1.314031627149369 | 1.134455484935953 | 0.7239690861897788 | 0.9513182763317971 | 0.8561882845464546 | 1.108032347864923 | 1.049716683623067
Cxcr3 | Profiler | 5.540437872443694 | 1.394743666350406 | 2.143546925072588 | 0.5904963307147661 | 1.511519928316227 | 1.441928871460227 | 1.164733586468458 | 1.822602561274282 | 1.939923820683464 | 1.858321348501824 | 2.5526575376579 | 1.498999601755543 | 1.714752073268342 | 1.236275260920952 | 1.187559666073411 | 1.079228236504429
Gata3 | Profiler | 4.346939450104226 | 0.7071067811865493 | 0.8766057213160365 | 0.5743491774985172 | 0.7557599641581134 | 0.8633395585744118 | 1.132883885295799 | 1.153485605060022 | 0.8741786204103715 | 0.8490962464468391 | 0.9087781164485188 | 1.10496448479991 | 0.8633395585744121 | 0.7149924931808445 | 1.354724977485367 | 1.613283518444254
Gata3 | qRT-PCR |  |  |  |  | 0.66 | 1.17 | 0.65 | 1.17 | 0.62 | 0.56 | 0.65 | 0.64 | 0.57 | 1.24 | 0.85 | 1.38 | 1.75 | 0.98 | 1.27 | 1.75
Gfi1 | Profiler | 3.917681190347712 | 2.173469725052118 | 2.657371628193029 | 1.071773462536294 | 1.210833083950553 | 0.9713074955416343 | 0.8585654364377541 | 0.8987551266110295 | 0.7933338431911431 | 0.7922348109249682 | 0.8247335486100802 | 0.7391812156369211 | 3.966866922953172 | 1.79751025322206 | 3.027233585939155 | 2.250116969377621
Icos | Profiler | 3.706352247561485 | 1.69349062472506 | 1.515716566510399 | 1.840375301249749 | 1.018185199764621 | 1.147107024226168 | 1.132883885295801 | 0.7770072687840491 | 0.6181376304604762 | 0.6524774738444789 | 0.6983392662336986 | 0.5680146323933437 | 1.392811480669033 | 1.244874235382169 | 1.787570324713575 | 1.25701337452183
IFN-g | Profiler | 188.7064598132612 | 7.78123957929829 | 7.464263932294468 | 12.38051994833911 | 49.38496560188582 | 59.63138747550546 | 4.027822200226879 | 0.3335557924194423 | 16.63327096280443 | 9.74052814930386 | 1.073260286393451 | 0.3988726199045155 | 19.67102187607327 | 98.0876774532981 | 82.02546714625218 | 0.7022224378689977
IFN-g | qRT-PCR |  |  |  |  | 359 | 348 | 5.85 | 0.3 | 31.16 | 20.31 | 0.79 | 0.6 | 12.97 | 77.69 | 33.28 | 0.21 | 16.23 | 12.18 | 5.21 | 1.2
Igsf6 | Profiler | 2.53151318794056 | 3.732131966147237 | 1.375541818139745 | 1.464085695945626 | 1.169587664051946 | 0.6363974676379708 | 0.7684375906440065 | 0.6811290173202429 | 1.288774630485015 | 1.286989247301269 | 1.406393199913117 | 2.646342879816234 | 1.611048582097499 | 0.9434382508953586 | 1.915870436445392 | 1.647182034535146
IL-10 | Profiler | 0.6372803136596329 | 2.188587402521483 | 1.189207115002724 | 0.5509525579383069 | 1.352848231050271 | 1.041021597684112 | 0.7120250977985347 | 0.7300160053272589 | 1.315854525029006 | 0.8790395609117844 | 1.496922986792688 | 1.059952783397279 | 0.7361134310840892 | 1.297738766939181 | 1.281647924160193 | 0.8235910172675743
IL-12b | Profiler | 1.021012125707191 | 0.7071067811865505 | 1.62450479271247 |  | 0.821310700752064 | 0.8055242910487499 | 0.8585654364377544 | 1.24487423538217 | 0.6181376304604747 | 0.5839836973065573 | 0.3491696331168491 | 0.5486659687896202 | 0.7944344000961042 | 0.4588199411556829 | 0.8753913296942635 | 1.580082623726754
IL-12b | qRT-PCR |  |  |  |  | 0.47 | 0.56 | 0.23 | 0.83 | 0.71 | 0.32 | 0.43 | 0.65 | 0.32 | 0.47 | 0.28 | 0.47 | 0.79 | 0.28 | 0.45 | 0.93
IL-12rb2 | Profiler | 6.062866266041615 | 2.013911100113444 | 2.887858391044995 | 0.6736167884328431 | 7.091053593558925 | 3.726961717219091 | 1.536875181288007 | 0.8327757708805451 | 6.84950627637164 | 2.977290510333076 | 1.767855062127337 | 1.832737289350697 | 13.52915659804022 | 3.37760354969512 | 12.1089343437566 | 3.18214593501967
IL-12rb2 | qRT-PCR |  |  |  |  | 3.73 | 5.06 | 0.72 | 0.54 | 1.63 | 1.24 | 0.82 | 0.81 | 1.84 | 2.5 | 1.78 | 0.52 | 3.71 | 1.42 | 1.37 | 1.09
IL-13 | Profiler | 0.2773923680169603 | 2.56685179512582 | 1 | 6.020986989644296 | 0.5648736072850628 | 1.326845140597166 | 0.8066417592221274 | 0.1588789611632083 | 4.304960499135193 | 4.039005190386753 | 7.022574380876143 | 8.597993382844008 | 0.1931776374119965 | 2.724516069280972 | 2.964934019605666 | 0.7371346086455504
IL-13 | qRT-PCR |  |  |  |  | 0.76 | 0.94 | 0.05 | 0.08 | 0.86 | 0.67 | 0.82 | 0.76 | 0.34 | 6.27 | 0.81 | 0.82 | 2.78 | 0.63 | 0.8 | 3.21
IL-13ra1 | Profiler | 0.5321850912266798 | 1.918528238650535 | 1.866065983073619 | 2.907945034640625 | 1.712376569092913 | 1.373636233429624 | 1.18920711500272 | 0.9699619103417344 | 2.291035795959512 | 2.401606854970411 | 1.755643595219037 | 1.428003979685061 | 3.155787363409857 | 0.9835020740262458 | 2.200757218732272 | 2.013911100113435
IL-15 | Profiler | 5.241573615433467 | 0.9526379980439371 | 0.7578582832551997 | 2.188587402521477 | 1.076240124783796 | 1.033830736247963 | 0.8066417592221271 | 0.6811290173202431 | 0.9434382508953582 | 0.9100388240808976 | 0.8362463999140612 | 0.8032939967570325 | 1.062895674358551 | 1.004167543238973 | 1.482467009802837 | 1.18920711500272
IL-17a | Profiler |  |  | 0.5396141182522136 | 2.188587402521478 | 4.072740799058483 | 3.6756515339162 | 0.517632461920689 | 0.09316939917493226 | 4.646034927702893 | 2.664749650184041 | 1.095811766447689 | 0.2613400250512912 | 1.308578071455008 | 11.36085886834739 | 10.46862457164007 | 1.156688183905288
IL-17a | qRT-PCR |  |  |  |  | 11.24 | 14.46 | 0.95 | 0.17 | 6.11 | 3.98 | 1.51 | 1.02 | 0.85 | 13.71 | 9.03 | 0.66 | 0.47 | 0.15 | 0.29 | 0.61
IL-18 | Profiler | 1.853176123780738 | 0.7900413118633771 | 0.4931163522466797 | 2.445280555384141 | 0.7770072687840491 | 0.8937851623567873 | 0.8010698775896238 | 0.9632618940201491 | 1.262252032112114 | 1.38895513576511 | 1.40639319991312 | 0.9753554615276406 | 0.918912883479048 | 1.068805991211006 | 1.123499903031644 | 1.404444875737996
IL-18bp | Profiler | 4.958830799755942 | 1.717130872875509 | 2.566851795125813 | 1.205807827690763 | 5.79978733151541 | 4.620343138214341 | 2.281527431736849 | 1.501079097514369 | 3.285242803008257 | 2.816688453824161 | 1.250062302585208 | 0.7759308542748545 | 7.61054621065438 | 3.262549973356029 | 5.198156249821748 | 2.056227653312132
IL-18bp | qRT-PCR |  |  |  |  | 7.39 | 6.49 | 1.28 | 1.69 | 3.1 | 2.38 | 1.06 | 0.89 | 3.56 | 2.73 | 1.68 | 0.44 | 3.27 | 0.68 | 1.46 | 1.07
IL-18r1 | Profiler |  | 0.668963777393057 | 0.5321850912266812 | 0.678302163723836 | 0.7770072687840487 | 0.7361134310840872 | 0.8010698775896195 | 0.6181376304604747 | 2.182527754109873 | 1.009751297596685 | 1.330529041080686 | 1.795020101334525 | 4.310932580174675 | 0.9050064628829042 | 0.1777596763234092 | 4.19886673449227
IL-2 | Profiler | 3.972369981748148 | 0.7219645977612487 | 1.777685362333143 | 2.549121254638532 | 1.24487423538217 | 3.006322956131129 | 0.939522749214012 | 0.7988519164491472 | 0.84440088742378 | 1.052631154709437 | 1.215879283183094 | 0.30228891895834 | 0.2479291826845731 | 0.8444008874237817 | 0.527776858818983 | 0.6506709277209678
IL-22 | qRT-PCR |  |  |  |  | 14.49 | 12.89 | 0.36 | 0.03 | 3.33 | 3.41 | 0.9 | 0.61 | 0.38 | 5.02 | 2.65 | 0.17 | 2 | 1.11 | 1.52 | 1.16
IL-23a | Profiler |  |  |  |  |  |  |  |  |  |  |  |  | 3.778988163988828 | 3.496714481641499 | 7.003130637554124 | 5.06302637588113
IL-23a | qRT-PCR |  |  |  |  | 2.62 | 3.66 | 0.11 | 0.35 | 0.74 | 0.69 | 1.1 | 1.49 | 0.29 | 1.34 | 0.93 | 0.36 | 20.04 | 2.77 | 2.42 | 3.82
IL-27 | Profiler |  | 1.140763715868427 | 6.773962498900223 | 0.737134608645553 |  |  |  |  | 1.543280175058935 | 1.562655760344637 | 1.126619228497277 | 0.9619274546647768 | 3.048289660679532 | 3.545526796779463 | 3.885229982166531 | 1.26575659397028
IL-27 | qRT-PCR |  |  |  |  | 6.58 | 7.78 | 0.61 | 0.75 | 2.21 | 2.08 | 1.04 | 1.56 | 2.69 | 5.12 | 2.42 | 0.46 | 6.13 | 0.68 | 0.93 | 1.75
IL27ra | Profiler | 2.297396709994069 | 1.404444875737999 | 1.375541818139745 | 0.9526379980439372 | 0.7249734164394248 | 0.8455722873775161 | 0.8950250709279723 | 1.018185199764621 | 0.6443873152425076 | 0.6524774738444791 | 0.6561056272959802 | 0.4678117489157001 | 0.9848664428955495 | 0.6954409858549937 | 1.556170353110839 | 1.827662900458802
IL-27ra | qRT-PCR |  |  |  |  | 0.89 | 1.43 | 0.83 | 1.13 | 0.47 | 0.39 | 0.68 | 0.53 | 0.37 | 0.57 | 0.47 | 0.41 | 1.24 | 0.74 | 1.05 | 0.79
IL-2ra | Profiler | 3.031433133020798 | 1.301341855441936 | 1.079228236504429 | 1.827662900458802 | 1.262252032112114 | 1.212512819061736 | 0.801069877589622 | 0.5381200623918978 | 1.410297960484388 | 1.052631154709437 | 1.142346246848543 | 0.501388217950538 | 0.5277768588189833 | 1.068805991211008 | 1.534746096000885 | 0.8010698775896219
IL-4 | Profiler | 1.658639091628884 |  |  |  |  |  |  |  | 0.2804858701326205 | 0.4072537815351074 | 3.2761467914393 | 0.1822511723520717 | 1.155085784553587 | 1.74835724082075 | 3.453358234297651 | 1.366040256754399
IL-4ra | Profiler | 2.462288826689834 | 0.9930924954370371 | 1.319507910772896 | 1.00695555005672 | 1.004167543238971 | 1.345367208521827 | 1.394743666350406 | 1.297738766939182 | 0.9835020740262476 | 0.9100388240808995 | 0.7179722553117169 | 1.097331937579243 | 1.462057448400032 | 1.061423208949793 | 2.294214048452331 | 2.265767770591597
IL-5 | Profiler |  |  |  |  | 0.2527880202128263 | 0.1681709010652284 | 0.5904963307147647 | 0.119576019761484 |  | 2.240778428388355 | 21.28846465729097 | 2.179504224426648 | 0.7310287242000157 | 1.511519928316225 | 2.37511933214682 | 2.887858391044992
IL-6 | Profiler | 6.821079134143649 | 1.729074462615735 | 3.010493494822141 | 2.584705661274986 | 4.395416870307517 | 3.244508622308179 | 0.7422617853145266 | 0.2527880202128263 | 7.600003062999078 | 3.874472756906502 | 0.8839275310636683 | 0.5921358059268144 | 4.620343138214319 | 4.646034927702878 | 5.532762516043843 | 1.071773462536291
IL-6 | qRT-PCR |  |  |  |  | 9.22 | 7.17 | 0.5 | 0.42 | 8.44 | 5.27 | 0.8 | 1.2 | 2.53 | 3.03 | 2.43 | 0.41 | 5.18 | 0.83 | 0.71 | 0.67
IL-7 | Profiler |  |  |  |  | 0.468460723436211 | 0.6820739174396759 | 1.06437018245336 | 0.3778799820790569 | 2.167451933689561 | 0.04556279308801801 | 0.9473700705992664 | 1.184271611853632 | 1.812523876534669 | 0.8802590135631518 | 1.229438867254582 | 1.057018040561384
IL-9 | Profiler |  |  |  |  |  |  |  |  |  |  |  |  | 0.08122119140346473 | 0.9369214468724247 | 1.402499250642396 | 0.3868912483855967
IL-r1 | Profiler |  | 14.62130320367045 | 8.876555776542775 | 1.591072967509835 | 0.6138678419575299 | 0.492433221447776 | 0.7684375906440065 | 0.5126336189442973 | 1.219255094076301 | 0.7443226275832687 | 1.029540083433993 | 0.442576882289772 | 1.787570324713572 | 0.631126016056057 | 1.354724977485367 | 1.464085695945626
Inha | Profiler |  |  |  |  | 0.2140470711366144 |  |  | 0.1726593060179828 | 1.460032010654519 | 9.279197236043466 | 23.62103049092528 | 0.7239690861897783 | 63.0314523453151 | 19.91798776611474 | 20.08435186052227 | 47.1766149533153
Irf1 | Profiler | 1.753211442632068 | 1.00695555005672 | 1.125058484688811 | 1.180992661429532 | 2.507066040881375 | 3.244508622308171 | 2.639015821545786 | 0.6443873152425076 | 2.323017463851446 | 2.936301272068203 | 1.50733490955838 | 0.7443226275832688 | 2.067661472495926 | 2.339175328103893 | 2.944453724336356 | 1.5157165665104
Irf1 | qRT-PCR |  |  |  |  | 3.52 | 4.63 | 2.92 | 0.54 | 2.48 | 2.39 | 1.15 | 0.56 | 1.46 | 2.14 | 1.22 | 0.49 | 1.99 | 1.3 | 1.83 | 1.43
Irf4 | Profiler | 3.580100283711899 | 1.602139755179248 | 1.071773462536294 | 1.049716683623067 | 2.16745193368956 | 1.929196369168277 | 1.93187265784969 | 2.404938497796351 | 0.5929572497054736 | 0.8032939967570344 | 0.6470728272414267 | 1.19250887233063 | 3.600007719174045 | 0.9500003828748846 | 3.477378240360082 | 3.138336391586999
[NAME] | Profiler | 2.203810231753217 | 0.9460576467255969 | 1.328685814096512 | 1.49484924863494 | 0.735093668194521 | 0.816768991110024 | 1.06437018245336 | 1.315854525029009 | 0.9176398823113637 | 0.6896804609135296 | 1.02242853060992 | 0.8374064875084244 | 0.636397467637971 | 0.6443873152425076 | 0.7999601283327511 | 0.8888426811665714
Jak2 | Profiler | 1.753211442632068 | 1.156688183905288 | 1.101905115876611 | 0.8585654364377543 | 1.219255094076302 | 1.085229371881809 | 1.079228236504427 | 0.9566081580918742 | 1.297738766939181 | 1.143930973203807 | 1.207480591486495 | 0.8432311102501112 | 0.9253044280378512 | 0.8385681844409303 | 2.111107435275932 | 2.027918959580063
Jak3 | Profiler | 6.821079134143669 | 0.668963777393057 | 0.5664419426479004 | 1.945309894824573 | 3.262549973356038 | 2.844154821174548 | 2.928171391891253 | 2.455471367830121 | 0.5307116044748966 | 0.6754870422678175 | 0.3326322602701705 | 0.8550021778919712 | 7.824507787578717 | 2.323017463851446 | 13.25073058135274 | 8.876555776542775
[NAME] | Profiler | 2.188587402521477 | 1.094293701260742 | 1.035264923841381 | 1.257013374521827 | 1.835279764622727 | 1.775222674538995 | 1.658639091628884 | 1.410297960484388 | 1.054091423290736 | 1.030968318794786 | 0.9025007274624282 | 0.9291606742509124 | 1.599920256665502 | 1.054091423290735 | 2.185555478201905 | 1.72907446261573
Maf | Profiler | 1.301341855441933 | 0.9460576467255968 | 1.356604327447673 | 1.094293701260739 | 0.8156374933390094 | 0.7159843706005721 | 0.8235910172675724 | 0.7878538860493455 | 0.597081593537295 | 0.501388217950538 | 0.5672277424679765 | 0.7391812156369195 | 0.8339310438249617 | 0.5020837716194854 | 1.108032347864926 | 1.424050195597069
Mapk8 | Profiler |  | 0.8705505632961266 | 1.140763715868425 |  | 2.559744827688613 | 1.969732885791103 | 3.363585661014857 | 2.036370399529241 | 1.227735683915059 | 2.005552871802151 | 1.743516479074122 | 1.674812975016853 | 0.3181987338189857 | 0.5847938320259735 | 0.4257268538744731 | 0.3977682418774601
Mapk9 | Profiler | 4.228072162245527 | 1.101905115876614 | 1.049716683623067 | 1.394743666350409 | 1.288774630485015 | 1.441928871460227 | 1.274560627319264 | 1.24487423538217 | 1.169587664051947 | 1.269270886019803 | 0.8021811663524854 | 0.9356234978313974 | 1.354724977485367 | 1.091263877054935 | 1.503161478065564 | 1.301341855441936
Nfatc1 | Profiler | 0.6689637773930581 | 0.8235910172675747 | 1.19747870461893 | 1.265756593970283 | 0.9632618940201488 | 0.9317404293047753 | 1.172834949231879 | 1.219255094076305 | 0.8863816991948653 | 0.7239690861897771 | 0.5791464030973201 | 0.8851537645795439 | 2.155466290087343 | 0.8385681844409306 | 2.358713184522431 | 2.329467172936916
Nfatc2 | Profiler | 0.6973718331752067 | 0.432268615653932 | 0.524858341811535 | 4.993322195606438 | 7.140375771783802 | 6.811629661991558 | 8.693878900208462 | 11.12705577367989 | 0.6811290173202429 | 0.6896804609135297 | 0.3794548132435137 | 1.468150636034101 | 7.663481745781564 | 1.57570777209869 | 8.803028874929081 | 12.38051994833913
Nfatc2ip | Profiler | 0.7269862586601566 | 0.9201876506248762 | 2.265767770591596 | 1.125058484688813 | 3.961371487732097 | 4.717426369044872 | 3.680750602499496 | 4.582071591919031 | 0.8681402276010404 | 0.8316220983749029 | 0.5517168700209781 | 1.200803427485204 | 0.900001929793512 | 0.6488693834695923 | 1.115739322324965 | 1.071773462536295
Nfatc3 | Profiler | 2.657371628193025 | 1.453972517320313 | 1.310393403858367 | 1.140763715868425 | 0.8156374933390097 | 0.8055242910487517 | 1.049716683623068 | 1.153485605060022 | 0.911301280637139 | 0.9753554615276386 | 1.207480591486492 | 0.8258776649357179 | 0.8281706613345444 | 0.7350936681945193 | 0.8455722873775159 | 0.8526348917679565
Nfkb1 | Profiler | 3.944930817973437 | 0.9659363289248475 | 1.239707699938986 | 0.8888426811665712 | 0.9176398823113637 | 0.9917167307382929 | 1.132883885295799 | 1.177722789594979 | 1.098854217576879 | 1.19250887233063 | 1.134455484935953 | 0.8374064875084266 | 1.220946512669132 | 0.9972312513520686 | 1.513616792969578 | 1.292352830637493
Pcgf2 | Profiler | 1.04971668362307 | 2.828427124746193 | 3.095129987084785 | 0.9012504626108289 | 1.371733288590588 | 1.041021597684109 | 0.9395227492140121 | 0.6355158446528301 | 1.169587664051946 | 0.7189682664234754 | 0.2683150715983628 | 0.421615555125055 | 12.97803448923269 | 5.486933154362402 | 11.53543097168182 | 12.46663327456804
Ptprc | Profiler | 2.602683710883872 | 1.013959479790031 | 1.729074462615733 | 1.19747870461893 | 1.420107358543424 | 1.566994373898681 | 1.057018040561379 | 1.121943480530482 | 0.9566081580918719 | 1.045360100205162 | 1.073260286393451 | 0.9227424926537964 | 1.876442393001339 | 1.16150873192572 | 1.825130977086902 | 1.693490624725056
Sftpd | Profiler
Socs1 | Profiler | 1.48452357062905 | 1.057018040561382 | 1.013959479790029 | 1.414213562373095 | 6.480027789056373 | 7.610546210654397 | 4.691339796927517 | 0.8621435450175966 | 2.940374672778077 | 3.615010907004341 | 0.8077607776096063 | 0.825877664935718 | 6.811629661991565 | 6.390815331593177 | 8.562309587412987 | 2.462288826689834
Socs1 | qRT-PCR |  |  |  |  | 14.6 | 17.25 | 5.27 | 1.28 | 4.7 | 5.08 | 1.05 | 0.84 | 2.72 | 8.91 | 4.48 | 1.07 | 3.49 | 2.62 | 2.81 | 1.61
Socs3 | Profiler | 3.863745315699379 | 1.042465760841123 | 1.986184990874075 | 1.214194884395048 | 1.532619960207779 | 1.714752073268342 | 1.26575659397028 | 0.7988519164491472 | 0.9972312513520684 | 0.9486843151404536 | 0.7695036100804311 | 0.6944775678825553 | 3.244508622308172 | 2.743466577181194 | 3.831740872890782 | 1.815038310634326
Socs5 | Profiler |  | 1.931872657849694 | 1.394743666350406 | 1.385109468110927 | 1.046810281974575 | 1.220946512669135 | 1.414213562373095 | 1.410297960484388 | 1.061423208949793 | 1.097331937579241 | 1.150291893350604 | 0.9163686446753507 | 1.345367208521827 | 0.7149924931808462 | 1.179356592261216 | 1.76540599258131
Spp1 | Profiler | 0.6372803136596317 | 0.4931163522466798 |  | 0.4263174458839793 | 0.6096275470381507 | 0.6964057403345183 | 0.7578582832551998 | 0.7350936681945192 | 1.046810281974575 | 0.7041721134560386 | 1.001387255711331 | 0.5880456251454816 | 1.762960315814337 | 0.892546971472978 | 1.115739322324965 | 1.741101126592249
Stat1 | Profiler | 2.969047141258099 | 0.8235910172675747 | 0.829319545814442 | 0.846745312362528 | 1.522033380839356 | 1.825130977086902 | 1.93187265784969 | 0.740206649000024 | 2.16745193368956 | 1.950710923055282 | 1.387030969131383 | 0.6754870422678175 | 2.111107435275933 | 1.597703832898294 | 2.510543982565285 | 1.424050195597073
Stat1 | qRT-PCR |  |  |  |  | 2.34 | 3.73 | 2.26 | 0.86 | 1.7 | 1.16 | 0.73 | 0.45 | 0.92 | 1.45 | 1.39 | 0.54 | 1.11 | 0.75 | 1.34 | 1.38
Stat4 | Profiler |  |  | 0.7684375906440086 |  |  |  |  |  | 1.039579435175277 | 1.217566018662986 | 4.475348404142844 |  | 1.392811480669035 | 1.011152080851303 | 3.267075964440096 | 1.972465408986721
Tbet | Profiler | 7.674112954602117 | 2.297396709994075 | 4.346939450104224 | 1.180992661429532 | 8.790833740615032 | 6.671448350599712 | 3.386981249450112 | 1.953417057924451 | 3.521036054252593 | 5.219819790755816 | 1.560490960347403 | 1.458009378816576 | 4.28115479370649 | 3.377603549695126 | 3.885229982166527 | 0.8827029962906545
Tbet | qRT-PCR |  |  |  |  | 3.73 | 5.22 | 0.93 | 1.17 | 2.27 | 1.89 | 2.12 | 2.04 | 1.72 | 3.74 | 0.92 | 0.38 | 9.5 | 2.27 | 3.37 | 0.86
Tcfcp2 | Profiler |  | 0.7320428479728149 | 1.101905115876611 | 0.9726549474122863 | 0.8621435450175948 | 0.8281706613345468 | 1.356604327447674 | 1.025267237888593 | 0.5456319385274668 | 0.6524774738444775 | 0.6293785867524281 | 0.5411123222628568 | 1.997229332202055 | 0.6533826269465868 | 1.633537982220043 | 1.840375301249749
Tgfb3 | Profiler | 1.505246747411067 | 1.189207115002724 | 1.00695555005672 | 1.569168195793504 | 1.429984986361689 | 1.738689120180045 | 1.905275996087875 | 2.022304161702607 | 1.098854217576877 | 1.437936532846955 | 1.036701101019718 | 1.74593518226752 | 2.49320238855019 | 0.9972312513520684 | 2.510543982565285 | 2.828427124746189
Tlr4 | Profiler |  | 0.5285090202806912 | 1.580082623726754 | 1.086734862526062 | 1.177722789594981 | 1.272794935275945 | 1.424050195597073 | 1.083725966844779 | 1.061423208949794 | 1.143930973203806 | 1.496922986792692 | 1.595490479618065 | 2.690734417043655 | 0.8987551266110299 | 2.278366754444234 | 2.000000000000005
Tlr6 | Profiler | 1.717130872875508 | 0.6830201283771987 | 1.443929195522497 | 1.866065983073619 | 1.848045144893646 | 1.775222674538999 | 1.283425897562904 | 0.7988519164491471 | 1.688801774847564 | 1.260503391582985 | 1.05848439487513 | 1.468150636034102 | 4.556733508888445 | 1.236275260920949 | 3.501565318777055 | 2.099433367246135
[NAME] | Profiler | 4.72397064571814 | 1.310393403858363 | 1.424050195597073 | 1.526259208960562 | 1.642621401504128 | 1.691144574755032 | 1.33792755478611 | 1.153485605060018 | 0.9632618940201464 | 0.9889709163809303 | 0.5555543643275458 | 1.016774673291954 | 1.775222674538995 | 1.334223169677769 | 1.187559666073411 | 1.248330548901611
TNF-a | Profiler | 2.657371628193023 | 1.536875181288013 | 2.188587402521483 | 1.394743666350406 | 2.404938497796358 | 2.01112116079694 | 1.021012125707194 | 0.5609717402652425 | 1.860899315406027 | 2.019502595193371 | 1.008352454894593 | 1.341642224882261 | 4.850051276246931 | 2.920064021309033 | 3.501565318777047 | 2.329467172936909
TNF-a | qRT-PCR |  |  |  |  | 3.44 | 4.2 | 0.73 | 0.84 | 1.41 | 1.28 | 0.92 | 1.09 | 1.76 | 1.38 | 1.75 | 0.82 | 3.31 | 0.84 | 0.84 | 1.13
Tnfrsf4 | Profiler | 1.613283518444255 | 0.8179020585577812 | 1.214194884395051 | 1.76540599258131 | 0.8385681844409305 | 1.055553717637966 | 0.8235910172675724 | 0.7300160053272605 | 1.098854217576879 | 0.8432311102501111 | 0.9876008614453721 | 0.6172813034467685 | 0.925304428037851 | 1.271031689305667 | 0.9645981845841385 | 0.946057646725597
Tnfrsf8 | Profiler |  | 0.6973718331752048 | 0.90751915531716 | 1.328685814096512 | 0.7824117817365115 | 1.077733145043672 | 0.7371346086455498 | 0.4280941422732284 | 0.8502741600641949 | 0.860949188338903 | 0.9876008614453717 | 0.2290921611688381 | 2.025109614700983 | 1.325007017045206 | 3.382289149510064 | 1.89211529345119
Tnfsf4 | Profiler | 2.158456473008859 | 1.248330548901615 | 6.727171322029733 | 1.239707699938989 | 1.760518027126301 | 1.019597682998192 | 1.057018040561382 | 0.9767085289622255 | 1.543280175058932 | 1.151887642349355 | 0.7381572028414147 | 0.7090700178397283 | 2.025109614700982 | 0.7557599641581116 | 1.290562490451778 | 1.156688183905289
Tyk2 | Profiler | 0.7526233737055336 | 1.164733586468458 | 1.292352830637497 | 1.214194884395048 | 0.6858666442952985 | 0.9317404293047729 | 1.094293701260739 | 0.9113012806371412 | 0.8681402276010427 | 1.097331937579241 | 1.08072540203935 | 1.016774673291954 | 1.115739322324963 | 0.8621435450175947 | 1.22943886725458 | 1.337927554786114
Yy1 | Profiler | 1.189207115002721 | 1.172834949231879 | 1.337927554786113 | 1.394743666350406 | 0.6625035085226032 | 0.8339310438249657 | 0.7684375906440067 | 1.177722789594982 | 1.219255094076302 | 1.009751297596686 | 1.455989548999085 | 1.030968318794787 | 0.7834971869493405 | 0.9767085289622282 | 0.8111271555770431 | 0.8408964152537167
*  In each experiment, 5 mice per vaccine group as well as 5 naive mice were challenged with 5 x 105 - 106 CFU LVS IP (determined by retrospective plate count), and survival monitored for a month; all naive mice died within 6 days of challenge, and the numbers of surviving mice within each vaccine group are shown.
‡  [NAME] expression was evaluated by RT PCR using [NAME] panel ("Profiler"); selected genes were further evaluated using specific primer/probe sets ("qRT-PCR").
~  All values are calculated fold differences of gene expression between vaccinated and non-vaccinated (naive) splenocytes.
%  In experiment 2, the same mRNA preparation was subjected to two independent PCR reactions (2a and 2b).
#  Mice vaccinated in experiments 2, 3, 4 and 6 were challenged, and spleens obtained for overlay and mRNA analyses, at ~ 6 weeks after vaccination, and mice vaccinated in experiments 1, 5 and 7 were analyzed and challenged ~ 12 weeks after vaccination. Exps 4 and 5 were vaccinated at the same time, and Exps 6 and 7 vaccinated at the same time.