Spreadsheet: 147b83df-4d00-44cd-9e89-700d7dc557e9
Sheet: CisPtTAXonly

Supplemental Table 2. List of identified changed proteins. Identified proteins typical for individual treatment with S2A: cisplatin (CisPt); S2B: paclitaxel (TAX). Proteins with overlap between CisPt and TAX are listed in S2C; between CisPt and DNR in S2D; between CisPt and DOXO in S2E; between CisPt and MTX in S2F; between TAX and DNR in S2G; between TAX and DOXO in S2H. Proteins with overlap between CisPt, DOXO and MTX are summarized in S2I; between CisPt, DNR and TAX in S2J; between CisPt, TAX and MTX in [NAME]; between TAX, DNR and MTX in S2L and between TAX, DOXO and MTX in S2M. Proteins from basic pH 6–11 are annotated as “b” beside spot number. The Gene Ontology biological process classification using PANTHER software is indicated by numbers 1 for cellular process, 2 for developmental process, 3 for generation of precursor metabolites and energy, 4 for immune system process/response to stimulus, 5 for metabolic process, 6 for reproduction, 7 for transport and 8 for un-annotated proteins
S2A | CisPt
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 8b | Phosphoglycerate kinase 1 | [NAME] | P00558 | 5 | 6-11 | ↑ | 1.93 | 0.0009
CisPt | 22b | Glucose-6-phosphate isomerase | GPI | P06744 | 5 | 6-11 | ↑ | 2.97 | 0.0029
CisPt | 34b | Inosine-5'-monophosphate dehydrogenase 2 | IMPDH2 | P12268 | 5 | 6-11 | ↑ | 1.66 | 0.0082
CisPt | 53b | Poly(rC)-binding protein 1 | PCBP1 | Q15365 | 5 | 6-11 | ↑ | 1.53 | 0.0069
CisPt | 83b | Septin-6 | SEPT6 | Q14141 | 1 | 6-11 | ↑ | 1.81 | 0.0093
CisPt | 83b | Tubulin beta chain | TUBB | P07437 | 1 | 6-11 | ↑ | 1.81 | 0.0093
CisPt | 129b | Polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1 | Q8TCS8 | 5 | 6-11 | ↓ | 1.75 | 0.0071
CisPt | 140 | 78 [NAME] glucose-regulated protein | HSPA5 | P11021 | 4 | 4-7 | ↑ | 1.43 | 0.003
CisPt | 144 | Prohibitin | PHB | P35232 | 1 | 4-7 | ↓ | 1.36 | 0.0003
CisPt | 209b | Phosphoglycerate kinase 1 | [NAME] | P00558 | 5 | 6-11 | ↑ | 2.5 | 0.0008
CisPt | 233 | Rab GDP dissociation inhibitor alpha | GDI1 | P31150 | 1 | 4-7 | ↑ | 1.83 | 0.0007
CisPt | 244 | Heterogeneous nuclear ribonucleoprotein H | HNRNPH1 | P31943 | 5 | 4-7 | ↑ | 1.39 | 0.0083
CisPt | 267 | Hematopoietic lineage cell-specific protein | HCLS1 | P14317 | 5 | 4-7 | ↑ | 1.46 | 0.0062
CisPt | 289b | Lamina-associated polypeptide 2, isoform alpha | TMPO | P42166 | 4 | 6-11 | ↓ | 1.48 | 0.0026
CisPt | 295 | Dynactin subunit 2 | DCTN2 | [NAME] | 1 | 4-7 | ↑ | 1.51 | 0.0046
CisPt | 334b | Electron transfer flavoprotein subunit beta | ETFB | P38117 | 3 | 6-11 | ↑ | 1.64 | 0.0134
CisPt | 341b | Phosphoglycerate kinase 1 | [NAME] | P00558 | 5 | 6-11 | ↑ | 1.67 | 0.0086
CisPt | 442 | Serine/threonine-protein phosphatase 2A 65 [NAME] regulatory subunit A alpha isoform | [NAME] | P30153 | 8 | 4-7 | ↑ | 1.71 | 0.0023
CisPt | 635 | Semenogelin-1 | SEMG1 | P04279 | 6 | 4-7 | ↑ | 3.51 | 0.004
CisPt | 671 | Charged multivesicular body protein 2a | CHMP2A | O43633 | 7 | 4-7 | ↑ | 1.64 | 0.0017
CisPt | 783 | Serine/threonine-protein phosphatase 2A 65 [NAME] regulatory subunit A beta isoform | PPP2R1B | P30154 | 8 | 4-7 | ↑ | 3.2 | 0.0056
CisPt | 791 | Phosphoserine phosphatase | PSPH | P78330 | 5 | 4-7 | ↑ | 1.72 | 0.0006
CisPt | 1025 | Serine/threonine-protein kinase 3 | STK3 | Q13188 | 5 | 4-7 | ↑ | 1.71 | 0.0017
CisPt | 1033 | Signal transducing adapter molecule 1 | STAM | Q92783 | 7 | 4-7 | ↑ | 2.23 | 0.0036
S2B | TAX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
TAX | 289 | Galactokinase | GALK1 | P51570 | 5 | 4-7 | ↑ | 1.36 | 0.0068
TAX | 291 | Peptidyl-prolyl cis-trans isomerase FKBP5 | FKBP5 | Q13451 | 4 | 4-7 | ↑ | 1.48593415805161 | 0.0008439706458596152
TAX | 311 | Protein disulfide-isomerase A3 | PDIA3 | P30101 | 5 | 4-7 | ↑ | 1.35626492100495 | 0.006432354747957114
TAX | 318 | Splicing factor, arginine/serine-rich 7 | SFRS7 | [NAME] | 5 | 4-7 | ↓ | 1.35 | 0.003393735134065379
TAX | 387 | Sorting nexin-6 | SNX6 | Q9UNH7 | 7 | 4-7 | ↑ | 1.57227541941424 | 0.00411909044940173
TAX | 387 | Heterogeneous nuclear ribonucleoprotein H | HNRNPH1 | P31943 | 5 | 4-7 | ↑ | 1.57227541941424 | 0.00411909044940173
TAX | 428 | Quinone oxidoreductase-like protein 1 | CRYZL1 | O95825 | 5 | 4-7 | ↑ | 1.59144516251255 | 0.002488022146074242
TAX | 569 | SUMO-activating enzyme subunit 2 | UBA2 | Q9UBT2 | 5 | 4-7 | ↑ | 1.37796368560769 | 0.00131729770802258
TAX | 583 | Dual specificity mitogen-activated protein kinase kinase 2 | MAP2K2 | P36507 | 5 | 4-7 | ↑ | 1.40454665222928 | 0.007520643838189067
TAX | 597 | [NAME]-coenzyme A transferase 1, mitochondrial | [NAME] | P55809 | 5 | 4-7 | ↑ | 1.38120524502422 | 5e-05
TAX | 645 | Gametocyte-specific factor 1 | GTSF1 | Q8WW33 | 8 | 4-7 | ↓ | 5.21 | 0.003910530133719003
TAX | 702 | 4-trimethylaminobutyraldehyde dehydrogenase | ALDH9A1 | P49189 | 5 | 4-7 | ↑ | 1.36 | 0.0041
TAX | 791 | Phosphoserine phosphatase | PSPH | P78330 | 5 | 4-7 | ↑ | 1.37649490853046 | 0.00813258664814931
TAX | 801 | Hypoxia up-regulated protein 1 | HYOU1 | Q9Y4L1 | 4 | 4-7 | ↑ | 1.47357679903589 | 0.007497573702949234
TAX | 1415 | Crk-like protein | CRKL | P46109 | 1 | 4-7 | ↓ | 2.11 | 0.00786213956366466
S2C | CisPt+TAX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 41 | Gamma-enolase | [NAME] | P09104 | 5 | 4-7 | ↑ | 1.74 | 0.0029
TAX | 41 | Gamma-enolase | [NAME] | P09104 | 5 | 4-7 | ↑ | 1.55060789320537 | 0.003797408630276888
CisPt | 382 | [NAME] | ANXA5 | P08758 | 1 | 4-7 | ↑ | 1.59 | 0.0017
TAX | 382 | [NAME] | ANXA5 | P08758 | 1 | 4-7 | ↑ | 1.43751916909279 | 0.001601747632912653
CisPt | 561 | Gamma-enolase | [NAME] | P09104 | 5 | 4-7 | ↑ | 2.13 | 0.0012
TAX | 561 | Gamma-enolase | [NAME] | P09104 | 5 | 4-7 | ↑ | 1.83617703528029 | 0.001200758668661872
S2D | CisPt+DNR
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 107b | Glucose-6-phosphate 1-dehydrogenase | G6PD | P11413 | 5 | 6-11 | ↑ | 1.98 | 0.0291
DNR | 107b | Glucose-6-phosphate 1-dehydrogenase | G6PD | P11413 | 5 | 6-11 | ↓ | 2.95 | 0.0013
S2E | CisPt+DOXO
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 63b | EH domain-containing protein 1 | EHD1 | Q9H4M9 | 7 | 6-11 | ↑ | 1.52 | 0.0013
DOXO | 63b | EH domain-containing protein 1 | EHD1 | Q9H4M9 | 7 | 6-11 | ↑ | 1.79 | 0.049
CisPt | 163b | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | ACADM | P11310 | 3 | 6-11 | ↓ | 1.67 | 0.0002
DOXO | 163b | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | ACADM | P11310 | 3 | 6-11 | ↓ | 1.45 | 0.0025
CisPt | 170b | Far upstream element-binding protein 2 | KHSRP | Q92945 | 5 | 6-11 | ↑ | 1.55 | 0.013
DOXO | 170b | Far upstream element-binding protein 2 | KHSRP | Q92945 | 5 | 6-11 | ↑ | 1.89 | 0.0013
CisPt | 592 | Ezrin | EZR | P15311 | 1 | 4-7 | ↑ | 2.08 | 0.0028
DOXO | 592 | Ezrin | EZR | P15311 | 1 | 4-7 | ↑ | 2.59 | 0.0071
S2F | CisPt+MTX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 615 | Transformer-2 protein homolog beta | [NAME] | P62995 | 5 | 4-7 | ↑ | 1.81 | 0.0002
MTX | 615 | Transformer-2 protein homolog beta | TRA2B | P62995 | 5 | 4-7 | ↓ | 1.93 | 0.0025
S2G | TAX+DNR
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
TAX | 407 | Protein phosphatase methylesterase 1 | PPME1 | Q9Y570 | 5 | 4-7 | ↑ | 1.49399730320625 | 0.003276692215478216
DNR | 407 | Protein phosphatase methylesterase 1 | PPME1 | Q9Y570 | 5 | 4-7 | ↑ | 1.44 | 0.0083
S2H | TAX+DOXO
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
TAX | 364 | 4-trimethylaminobutyraldehyde dehydrogenase | ALDH9A1 | P49189 | 5 | 4-7 | ↑ | 1.44249821339337 | 0.003293612146238467
DOXO | 364 | 4-trimethylaminobutyraldehyde dehydrogenase | ALDH9A1 | P49189 | 5 | 4-7 | ↑ | 1.51 | 0.0033
TAX | 699 | Rho GDP-dissociation inhibitor 2 | ARHGDIB | P52566 | 1 | 4-7 | ↓ | 1.74 | 0.00209437506562371
DOXO | 699 | Rho GDP-dissociation inhibitor 2 | ARHGDIB | P52566 | 1 | 4-7 | ↓ | 2 | 0.0015
S2I | CisPt+DOXO+MTX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 25b | Peptidyl-prolyl cis-trans isomerase | PPIA | P62937 | 4 | 6-11 | ↓ | 1.46 | 0.0069
DOXO | 25b | Peptidyl-prolyl cis-trans isomerase | PPIA | P62937 | 4 | 6-11 | ↓ | 1.85 | 0.0023
MTX | 25b | Peptidyl-prolyl cis-trans isomerase | PPIA | P62937 | 4 | 6-11 | ↓ | 1.7 | 0.0025
S2J | CisPt+DNR+TAX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 126 | Cofilin-1 | CFL1 | [NAME] | 1 | 4-7 | ↑ | 2.13 | 0.0013
TAX | 126 | Cofilin-1 | CFL1 | [NAME] | 1 | 4-7 | ↑ | 1.5230956024002 | 0.009005125280540649
DNR | 126 | Cofilin-1 | CFL1 | [NAME] | 1 | 4-7 | ↑ | 2.01 | 0.0045
[NAME] | CisPt+TAX+MTX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
CisPt | 109b | Heterogeneous nuclear ribonucleoproteins A2/B1 | HNRNPA2B1 | P22626 | 5 | 6-11 | ↓ | 1.42 | 0.0064
TAX | 109b | Heterogeneous nuclear ribonucleoproteins A2/B1 | HNRNPA2B1 | P22626 | 5 | 6-11 | ↑ | 1.47 | 0.0074
MTX | 109b | Heterogeneous nuclear ribonucleoproteins A2/B1 | HNRNPA2B1 | P22626 | 5 | 6-11 | ↓ | 1.87 | 0.0071
S2L | TAX+DNR+MTX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
TAX | 413 | NADH-ubiquinone oxidoreductase 75 [NAME] subunit, mitochondrial | NDUFS1 | P28331 | 3 | 4-7 | ↑ | 1.45323455629415 | 0.007360622208364642
DNR | 413 | NADH-ubiquinone oxidoreductase 75 [NAME] subunit, mitochondrial | NDUFS1 | P28331 | 3 | 4-7 | ↓ | 1.37 | 0.0015
MTX | 413 | NADH-ubiquinone oxidoreductase 75 [NAME] subunit, mitochondrial | NDUFS1 | P28331 | 3 | 4-7 | ↑ | 1.6 | 0.0073
S2M | TAX+DOXO+MTX
Drug | Spot No. | Protein name | [NAME] | UniProt No. | Biological function | pH | Change | Fold change | p-value
TAX | 153 | D-3-phosphoglycerate dehydrogenase | PHGDH | O43175 | 5 | 4-7 | ↑ | 1.57399534615219 | 0.004887617713432935
DOXO | 153 | D-3-phosphoglycerate dehydrogenase | PHGDH | O43175 | 5 | 4-7 | ↑ | 1.68 | 0.0068
MTX | 153 | D-3-phosphoglycerate dehydrogenase | PHGDH | O43175 | 5 | 4-7 | ↑ | 1.9 | 0.0097