Spreadsheet: 1037289e-b1b5-417c-8d61-48439e5718ac
Sheet: Sheet1

Amplicon | Primer | Primer sequence | Product size | Gene ID (BeeBase) | Unnamed: 5
[NAME] | stanex5_F2 | GCAGAATTCGATGTTTTAGGTTTTATTTTGAAG | 443 | GB18207 | [NAME] exon 5 (protocadherin-like, laminin-G domain)
 | stanex5_R2 | CGCAAGCTTAATATTACTAACAATCCCAACCAA
 |  |  |  |  | [NAME] exon 6 (protocadherin-like, laminin-G domain)
stanex6 | stanex6_F2 | GCAGAATTCTAGGTTTGGATGTAGTAATTTTAG | 310 | GB18207
 | stanex6_R2 | CGCAAGCTTTTCTTAACATTTAATTCACCTTAC
[NAME] | lrrk_F2 | [NAME] | 580 | GB18525 | Lrrk/Leucine-rich repeat kinase
 | lrrk_R2 | CGCAAGCTTAAACCTAATAAAACTAATTATAAC
Tap42 | tap42_F2 | GCAGAATTCGAATGTATATTGTTAATGTTGTTG | 366 | GB15132 | Tap42/Two A-associated protein of 42kDa
 | [NAME] | CGCAAGCTTCTTCATCATCTATATTTAAATCAA
RhoGEF | RhoGEF_F2 | GCAGAATTCGAGAAGTTTGAGTTTTGTAGGAGA | 626 | GB18941 | CG10188/Rho guanyl-nucleotide exchange factor
 | RhoGEF_R2 | CGCAAGCTTAACATCTCTTTATTCTACTACCAA
syd | syd_F2 | GCAGAATTCTAAAGTAGATGAATTTATAAGGTA | 407 | GB15356 | syd/jnk interacting protein
 | syd_R2 | CGCAAGCTTTCTAACTACTTCTTAAAATTCCAT
mcm5 | mcm5_F2 | GCAGAATTCTAATGTTTGGATTATTAGTTGGTA | 526 | GB12920 | Mcm5 (APTase)
 | mcm5_R2 | CGCAAGCTTATTAAAAATCATCCACATTATCCA
sertsyn | sertsyn_F2 | GCAGAATTCTTGGTAATGTATGTAAAATAATTG | 441 | GB11061 | [NAME] synthetase
 | sertsyn_R2 | CGCAAGCTTAAAACTTTATCCATCTAATATCTA
lap | lap_F2 | GCAGAATTCTATTTGTATGTTATAATGATGGTA | 349 | GB14848 | lap/PI-binding clathrin assembly protein
 | [NAME] | CGCAAGCTTTCAAATATATCTCCTTTATCAATA
Yhhn | Yhhn_F2 | GCAGAATTCTGGAATGTGTATGTTTGTTTTAGT | 145 | GB18602 | YhhN-like TM protein
 | Yhhn_R2 | GCAAGCTTATATAACATACATATCTTACCTAA
[NAME] | RhoGAP_F2 | GCAGAATTCAATTTAGTAATGGTGATGGTATTA | 346 | GB13464 | RhoGAP93B
 | [NAME] | CGCAAGCTTCAATAATAAACATCCATAAATCTA
ucf | ucf_F2 | GCAGAATTCTTAAGAAGTTGTTGATTGGTTGAA | 325 | GB18798 | CG9934/ubiquitin conjugation factor
 | ucf_R2 | CGCAAGCTTACACCTTCTCAATAAAACAAAATC
nadrin | nadrin_F2 | GCAGAATTCTATTTAGTGGATAAATAATAGGTT | 423 | GB16176 | nadrin
 | nadrin_R2 | CGCAAGCTTATCCTAAAAATTAATACTTTCATA
 |  |  |  | GenBank entry NC_001566.1 used for mito genes primer desing
[NAME] | mito2_F2 | GCAGAATTCATTAGGATTTATAGTTATATTAAG | 223 | COX3
 | mito2_R2 | CGCAAGCTTATATAAACCATAAAATCCTATTAC
mito4 | mito4_F2 | GCAGAATTCAAATATTAATTGTTTATTAGTTTG | 188 | ND4
 | mito4_R2 | CGCAAGCTTCAATTTCATCTATTATTCATATAA
[NAME] | mito5_F2 | [NAME] | 243 | Cyt B
 | mito5_R2 | CGCAAGCTTATATACAAATAAAAAATATCATTC
mito7 | mito7_F2 | GCAGAATTCTTAATTGTATTGATTTTAAAATAG | 331 | ND1
 | mito7_R2 | CGCAAGCTTTATATTCAAAATCATAAAAATCCT
Primers used for Real Time experiment shown in Figure 4
forward primer - common for both isoforms  F
 | AAGTATTGGTCCAAAGTTAGTC
 | reverse primer for "short" isoform  RS
 |  | AAGATCGAATTTCATGTGATTC
 | reverse primer for "long" isoform [NAME]
 |  | TTCGTCTCGAAAAGGTATATTC
 |  | PCR cycling
 |  | Hold (initial denaturation): 94 deg, 2'; Cycle: 94 deg, 15"; 63 deg, 20"; 72 deg, 30"; 40x; Followed by standard melt profile.  Tubes: 0.1 ml Rxn volume: 20 ul; Primers: 20 pmol ea; 1.25 u Promega Go Taq polymerase; [NAME] buffer; 2.5 mM MgCl2; 0.1 mM dNTP; 1:40000 SYBR I. Hold (initial denaturation): 94 deg, 2'; Cycle: 94 deg, 15"; 63 deg, 20"; 72 deg, 30"; 40x; Followed by standard melt profile.  Tubes: 0.1 ml Rxn volume: 20 ul; Primers: 20 pmol ea; 1.25 u Promega Go Taq polymerase; [NAME] buffer; 2.5 mM MgCl2; 0.1 mM dNTP; 1:40000 SYBR I. Hold (initial denaturation): 94 deg, 2'; Cycle: 94 deg, 15"; 63 deg, 20"; 72 deg, 30"; 40x; Followed by standard melt profile.  Tubes: 0.1 ml Rxn volume: 20 ul; Primers: 20 pmol ea; 1.25 u Promega Go Taq polymerase; [NAME] buffer; 2.5 mM MgCl2; 0.1 mM dNTP; 1:40000 SYBR I.