Spreadsheet: 6ec0baa7-3a5b-46e1-b0d1-b70bd3337f2c
Sheet: Sheet1

PCR nr. | Region | PCR primer | Sequence | Position | Seq. primer | Sequence.1 | Position.1
1 | 497-1359 | HXB2startfor | 5'- GCT AAC TAG GGA ACC CAC TGC TT -3' | 497-519 | S667 | 5'- CTT AAG CCT CAA ATA AGC TTG AA -3' | 517-539
 |  | HXB2GAGrev | 5'- TGT GTT TAG CAT GGT GTT TAA ATC TTG -3' | 1359-1333 | 5'LTR-3 | 5'- CAG CCT TCT GAT GTT TCT AA -3' | 959-940
2 | 890-1539 | 5'MA1 | 5'- TAG TAT GGG CAA GCA GGG AG -3' | 890-909 | 5'MA2 | 5'- TAT AAT ACA GTA GCA ACC CTC TAT TGT G -3' | 1024-1051
 |  | 3'MA3 | 5'- CAT CCA TCC TAT TTG TTC CTG AAG -3' | 1539-1516 | 3'MA2 | 5'- TCT GCA GCT TCC TCA TTG ATG GT -3' | 1421-1399
3 | 1318-1867 | cleams-1 | 5'- GAA GGA GCC ACC CCA CAA GAT -3' | 1318-1338 | SK145 | 5'- AGT GGG GGG ACA CA AGC AGC CAT -3' | 1359-1382
 |  | cleamrev-1 | 5'- CCT TAT GGC CGG GTC CTC CTA CT -3' | 1867-1845 | cleamrev2 | 5'- TTC TTC TAG TGT AGC CGC TGG TCC -3' | 1824-1801
4 | 1737-2734 | 5'clea-1 | 5'- GAT GAC AGA AAC CTT GTT GGT CC -3' | 1737-1759 | 5'clea-2 | 5'- AAA TGA TGA CAG CAT GTC AGG G -3' | 1823-1844
 |  | 3'prot-1 | 5'- GCA AAT ACT GGA GTA TTG TAT GGA TTT TCA GG -3' | 2734-2703 | PR5 | 5'- AGC CAA CAG CCC CAC CAG -3' | 2150-2167
 |  |  |  |  | 3'CS-2 | 5'- TCT CTT CTG GTG GGG CTG TTG GCT C -3' | 2173-2149
 |  |  |  |  | PR2 | 5'- CTT TTG GGC CAT CCA TTC -3' | 2610-2593
5 | 2377-3539 | RT18 | 5'- GGA AAC CAA AAA TGA TAG GGG GAA TTG GAG -3' | 2377-2406
 |  | RT21 | 5'- CTG TAT TTC TGC TAT TAA GTC TTT TGA TGG -3' | 3539-3510
5-nested | 2454-3462 | RT19 | 5'- GGA CAT AAA GCT ATA GGT ACA G -3' | 2454-2475 | RT19 | 5'- GGA CAT AAA GCT ATA GGT ACA G -3' | 2454-2475
 |  | RT20 | 5'- CTG CCA GTT CTA GCT CTG CTT C -3' | 3462-3441 | RT19new | 5'- GGA CCT ACA CCT GTC AAC ATA ATT GG -3' | 2484-2509
 |  |  |  |  | RT8 | 5'- GTT CAA TTA GGA ATA CC -3' | 2817-2833
 |  |  |  |  | BRT | 5'- GGA TGG AAA GGA TCA CC -3' | 3003-3019
 |  |  |  |  | BRRT | 5'- GGT GAT CCT TTC CAT CC-3' | 3019-3003
 |  |  |  |  | KRT | 5'- CAG GAT GGA GTT CAT AAC -3' | 3258-3241
6 | 3297-4405 | RT23 | 5'- GAC AGC TGG ACT GTC AAT GAC [NAME] -3' | 3297-3323 | RT20rev | 5'- GAA GCA GAG CTA GAA CTG GCA G -3' | 3441-3462
 |  | 3'RT-INT1 | 5'- CCT GGA CTA CAG TCT ACT TG -3' | 4405-4386 | RT15 | 5'- TTT AGG AGT CTT TCC CC -3' | 3713-3697
 |  |  |  |  | RT2 | 5'- GGA AAA GCA GGR TAT G -3' | 3906-3921
 |  |  |  |  | RT2rev | 5'- CAT AYC CTG CTT TTC C -3' | 3921-3906
 |  |  |  |  | RT22 | 5'- AGG TTA AAA TCA CTA GCC ATT GCT CTC C -3' | 4312-4285
7 | 4058-5264 | 5'INT-OUT | 5'- AGG AAT CAT TCA AGC ACA ACC AGA T -3' | 4058-4082 | 5'INT-IN | 5'- GTC TAC CTG GCA TGG GTA CCA GCA C -3' | 4140-4164
 |  | 3'INT-OUT | 5'- CCT GTA TGC AGA CCC CAA TAT GTT GTT -3' | 5264-5238 | INT-1 | 5'- GGA GAA GCC ATG CAT GG -3' | 4368-4384
 |  |  |  |  | INT-6 | 5'- GCC ATC TTC CTG CTA -3' | 4554-4540
 |  |  |  |  | INT-3 | 5'- TTC GGG TTT ATT ACA G -3' | 4897-4912
 |  |  |  |  | INT-8 | 5'- TGT TTT CCA TAA TCC -3' | 5050-5036
 |  |  |  |  | 3'INT-OUT | 5'- CCT GTA TGC AGA CCC CAA TAT GTT GTT -3' | 5264-5238
8 | 5143-6090 | INT-10 | 5'- GCT AGG GGA TGG TTT -3' | 5143-5157 | INT-10 | 5'- GCT AGG GGA TGG TTT -3' | 5143-5157
 |  | [NAME] | 5'- ACT ATT GCT ACT ATT GGTAT -3' | 6090-6071 | INT-11 | 5'- TAG CCC TAG GTG TGA A -3' | 5427-5442
 |  |  |  |  | INT-NCOI | 5'- TGT TGC CCT AAG CCA TGG AG -3' | 5692-5673
 |  |  |  |  | LA64MOD | 5'- GGT TGC GTT ACA TGT ACT AC -3' | 6069-6050
9 | 5933-7389 | OUT45 | 5'- CAA GTT TGT TTC ATA ACA AAA -3' | 5933-5953 | LA45MOD | 5'- GCC TTA GGC ATC TCC TAT GGC -3' | 5954-5974
 |  | JA170 | 5'- GTG ATG TAT TRC ART AGA AAA ATT C -3' | 7389-7365 | ENV-11 | 5'- TGT GGG TCA CAG TCT ATT ATG -3' | 6325-6345
 |  |  |  |  | ENV-12 | 5'- TGG GTT GGG GTC TGT GGG TA -3' | 6467-6448
 |  |  |  |  | JA-167 | 5'- TAT CYT TTG AGC CAA TTC CYA TAC A -3' | 6847-6871
 |  |  |  |  | ENV-10 | 5'- ACA TTG TAC TGT GCT GAC AT -3' | 6965-6946
 |  |  |  |  | [NAME] | 5'- AGA AAA ATT CYC CTC YAC AAT TAA A-3' | 7374-7350
10 | 7314-7992 | ENV-1 | 5'- TCC TCA GGA GGG GAC CCA GA -3' | 7314-7333 | ENV-3 | 5'- GGG GAC CCA GAA ATT [NAME] -3' | 7323-7342
 |  | ENV-2 | 5'- GAT CCT TTA GGT ATC TTT CCA CAG -3' | 7992-7969 | ENV-4 | 5'- TCT TTC CAC AGC CAG GAT TC -3' | 7979-7960
11 | 7833-8816 | ENV-5 | 5'- CTG ACG GTA CAG GCC AGA CA -3' | 7833-7852 | ENV-13 | 5'- CAA TTT GCT GAG GGC TAT TG -3' | 7883-7902
 |  | NEF-3 | 5'- TTT GAC CAC TTG CCA CCC AT -3' | 8816-8797 | ENV-9 | 5'- GTT TAA GAA TAG TTT TTG CTG TAC TTT -3' | 8305-8331
 |  |  |  |  | ENV-8 | 5'- TAT CCC TGC CTA ACT CTA TTC A -3' | 8359-8338
 |  |  |  |  | ENV-7 | 5'- CCT TTC CAA GCC CTG TCT TA -3' | 8783-8764
12 | 8685-9186 | 5'NEF-1 | 5'- GCA GTA GCT GAG GGG ACA GA -3' | 8685-8704 | 5'NEF-2 | 5'- ATG GGT GGC AAG TGG TCA AA -3' | 8797-8816
 |  | 3'NEF-1 | 5'- CCC TGG TGT GTA GTT CTG C -3' | 9186-9168 | 3'NEF-2 | 5'- TCC AGT CCC CCC TTT TCT TT -3' | 9089-9070
13 | 9016-9719 | NEF-4 | 5'- GTA CCT TTA AGA CCA ATG ACT TAC -3' | 9016-9039 | NEF-5 | 5'- CTT ACA AGG CAG CTG TAG AT -3' | 9035-9054
 |  | 3'LTR-1 | 5'- TGC TAG AGA TTT TCC ACA CTG ACT AA -3' | 9719-9694 | 3'LTR-2 | 5'- TAA AAG GGT CTG AGG GAT CT -3' | 9696-9677