Spreadsheet: a2f6be1d-f2f3-4daa-a5d9-ad6148d89d2f
Sheet: Sheet1

Measurements | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11
worm | worm | rat |  | worm embryos in utero (on DMSO) |  |  | worm embryos in utero (on 5uM nemadipine) |  |  | Rat Cav1.2 | Rat Cav1.2
[NAME] | polymorphism | polymorphism |  | avg | sem |  | avg | sem |  | IC50 on nifedipine | [NAME] (residual current)
wild type |  |  |  | 11.55 | 0.4728135272799911 |  | 18.1 | 0.6723877327538741 |  | 0.12679 | -0.0106
n2368 | G365R | na |  | 3.95 | 0.2944665113655361 |  | 7.45 | 0.45
ad1006 | unknown | na |  | 20.75 | 0.8999268976159293 |  | 2.75 | 0.6107588032844664
n582 | A899H | na |  | 28.35 | 0.9354846759553922 |  | 18.2 | 0.5311358236439097
tr81 | A236V | A283V |  | 10.8 | 0.3947017528263715 |  | 15.55 | 0.545194315914301 |  | 0.28233 | 0.0451
tr73 | A615V | S666V |  | 8.7 | 0.5670793131197973 |  | 11.25 | 0.5123475382979799 |  | 0.1839 | 0.156
tr93 | A615V | S666V |  | 10.25 | 0.4694621782871388 |  | 10.7 | 0.5670793131197969
tr79 | A702T | A752T |  | 7.1 | 0.3831998241951029 |  | 12 | 0.5380275868489703 |  | 0.15182 | 0.0897
tr132 | A702T | A752T |  | 9.25 | 0.4095890109545626 |  | 13.65 | 0.5994515036791203
tr76 | A702V | A752V |  | 8.75 | 0.4405439221785054 |  | 11.35 | 0.448828885457535 |  | 0.82181 | 0.0617
tr86 | A702V | A752V |  | 11.35 | 1.054501628358405 |  | 15.5 | 1.14363225594686
tr92 | M1055L | M1160L |  | 7.9 | 0.3900067475799548 |  | 14.15 | 0.4546542931896607 |  | 0.14572 | 0.431
tr69 | M1056I | na |  | 6.35 | 0.2436023506930049 |  | 10.75 | 0.5706644326895116
tr89 | M1056I | na |  | 6.7 | 0.4110320771193566 |  | 13.1 | 0.5978029951853971
tr71 | V1060L | V1165L |  | 9 | 0.3162277660168379 |  | 21.85 | 0.9010958241000366 |  | 4.60246 | 0.351
tr74 | T1066I | T1171I |  | 13.4 | 0.71229798613546 |  | 20.15 | 0.9098149030955348 |  | 0.09629 | 0.193
The correlation between the strength of the mutant phenotype (off drug) and the degree of resistance to nemadipine.
worm | worm | rat |  | [NAME] |  |  | embryos 5uM
[NAME] | polymorphism | polymorphism |  | avg | strenght of phenotype (egl (wt) over egl(mut) |  | avg | amount of resistance ((mut(egl(nema))/egl(non-nema))/(wt(egl(nema))/egl(non-nema))
wild type |  |  |  | 11.55 |  |  | 18.1
n2368 | G365R | na |  | 3.95 | 2.924050632911392 |  | 7.45 | 1.203622175728838
ad1006 | unknown | na |  | 20.75 | 0.5566265060240965 |  | 2.75 | 0.08457569909504004
n582 | A899H | na |  | 28.35 | 0.4074074074074074 |  | 18.2 | 0.409684306727489
tr81 | A236V | A283V |  | 10.8 | 1.069444444444444 |  | 15.55 | 0.9188352360017964
tr73 | A615V | S666V |  | 8.7 | 1.327586206896552 |  | 11.25 | 0.8252096032392228
tr93 | A615V | S666V |  | 10.25 | 1.126829268292683 |  | 10.7 | 0.6661789655548119
tr79 | A702T | A752T |  | 7.1 | 1.626760563380282 |  | 12 | 1.078583819445069
tr132 | A702T | A752T |  | 9.25 | 1.248648648648649 |  | 13.65 | 0.9417202780317012
tr76 | A702V | A752V |  | 8.75 | 1.32 |  | 11.35 | 0.8277874008569606
tr86 | A702V | A752V |  | 11.35 | 1.017621145374449 |  | 15.5 | 0.8714988937586623
tr92 | M1055L | M1160L |  | 7.9 | 1.462025316455696 |  | 14.15 | 1.143037166883426
tr69 | M1056I | na |  | 6.35 | 1.818897637795276 |  | 10.75 | 1.080353149857544
tr89 | M1056I | na |  | 6.7 | 1.723880597014925 |  | 13.1 | 1.247749764261018
tr71 | V1060L | V1165L |  | 9 | 1.283333333333333 |  | 21.85 | 1.549315748422322
tr74 | T1066I | T1171I |  | 13.4 | 0.8619402985074627 |  | 20.15 | 0.9596243415976912
 |  |  |  |  |  |  | correlation coefficient (strenght of phenotype vs. resistance) = | 0.6414981572378152
The correlation between worm resistance to nemadipine and the IC50 of the rat CaV1.2 mutation on nefidipine.
worm | worm | rat |  | Rat Cav1.2
[NAME] | polymorphism | polymorphism |  | IC50 on nifedipine |  |  | worm resistance to nemadipine
wild type |  |  |  | 0.12679 |  |  | 1
tr81 | A236V | A283V |  | 0.28233 |  |  | 0.9188352360017964
tr73* | A615V | S666V |  | 0.1839 |  |  | 0.746
tr79* | A702T | A752T |  | 0.15182 |  |  | 1.009
tr76* | A702V | A752V |  | 0.82181 |  |  | 0.849599
tr92 | M1055L | M1160L |  | 0.14572 |  |  | 1.143037166883426
tr71 | V1060L | V1165L |  | 4.60246 |  |  | 1.549315748422322
tr74 | T1066I | T1171I |  | 0.09629 |  |  | 0.9596243415976912
 |  |  |  |  |  | correlation coefficient (worm resistance vs. rat IC50) = | 0.8366572253918871
The correlation between worm resistance to nemadipine and the residual current of the rat CaV1.2 mutation on nefidipine.
worm | worm | rat |  | Rat Cav1.2
[NAME] | polymorphism | polymorphism |  | [NAME] (residual current) |  |  | worm resistance to nemadipine
wild type |  |  |  | -0.0106 |  |  | 1
tr81 | A236V | A283V |  | 0.0451 |  |  | 0.9188352360017964
tr73* | A615V | S666V |  | 0.156 |  |  | 0.746
tr79* | A702T | A752T |  | 0.0897 |  |  | 1.009
tr76* | A702V | A752V |  | 0.0617 |  |  | 0.849599
tr92 | M1055L | M1160L |  | 0.431 |  |  | 1.143037166883426
tr71 | V1060L | V1165L |  | 0.351 |  |  | 1.549315748422322
tr74 | T1066I | T1171I |  | 0.193 |  |  | 0.9596243415976912
 |  |  |  |  |  | correlation coefficient (worm resistance vs. rat residual current) = | 0.6301587367009069
*Note that the worm measurements for these polymorphisms is an average of the
measurements for two alleles (i.e. for A615V, we measured the number of embryos in utero for two alleles, tr73 and tr93)