Spreadsheet: 8198c104-e8ff-46b2-92e1-96faea9eeb0c
Sheet: S4 primers

 | Table S4  Oligonucleotides for ovine TMEM154 PCR, RT-PCR, and DNA sequencing
 | Primer numbera | DNA sequenceb | Primer binding region |  | Primer orientation | Position in GenBank Accession HM355886 | Function | Tm (oC) | DMSOc (4%) | STS length (bp)
 |  |  |  | Targeted elements
 | 84253 | [NAME] [NAME] | 5' UTR | exon 1 | sense | 4560-4576 | PCR amplification, DNA sequencing | 56 | + | 914
 | 83023 | [NAME] tca tta gtc aca atc aac | intron 1 | exon 1 | antisense | 5465-5485 | PCR amplification, DNA sequencing | 56 | + | 914
 | 84253 | [NAME] [NAME] | 5' UTR | exon 1 | sense | 4560-4576 | PCR amplification, DNA sequencing | 56 | + | 588
 | 83623 | gcc cat taa agc cgg t | intron 1 | exon 1 | antisense | 5132-5147 | PCR amplification, DNA sequencing | 56 | + | 588
 | 84495 | cgc gtt tca gcg gga ctg aca cc | 5' UTR | exon 1 | sense | 4722-4744 | DNA sequencing | 62 | - | nad
 | 83020 | agt [NAME] | intron 1 | exon 1 | antisense | 4926-4945 | DNA sequencing | 62 | - | na
 | 86824 | tcc att tcc ttt acc taa aag t | intron 1 | exon 2, 3 | sense | 28750-28771 | PCR amplification, DNA sequencing | 60 | - | 1048
 | 86826 | act [NAME] aat tac ata ag | intron 3 | exon 2, 3 | antisense | 29778-29797 | PCR amplification, DNA sequencing | 60 | - | 1048
 | 87811 | gcc ttt gtg gga gat tta | intron 1 | exon 2, 3 | sense | 28921-28938 | PCR amplification, DNA sequencing | 57 | - | 745
 | 87814 | aat ttt gga aaa tgt cac tga | intron 3 | exon 2, 3 | antisense | 29645-29665 | PCR amplification, DNA sequencing | 57 | - | 745
 | 83658 | [NAME] ttt atg ttc aat ca | intron 1 | exon 2, 3 | sense | 28985-29004 | PCR amplification, DNA sequencing | 56 | - | 771
 | 83659 | gag atg [NAME] gtg tgt ttc | intron 3 | exon 2, 3 | antisense | 29735-29755 | PCR amplification, DNA sequencing | 56 | - | 771
 | 85484 | gag ggt aag ttt cag atc att g | intron 1 | exon 2, 3 | sense | 29070-29091 | DNA sequencing | 62 | - | na
 | 85485 | [NAME] tgc ttc act taa a | intron 3 | exon 2, 3 | antisense | 29602-29623 | DNA sequencing | 62 | - | na
 | 83648 | aag [NAME] gac ag | intron 3 | exon 4 | sense | 36851-36867 | PCR amplification, DNA sequencing | 58 | - | 1185
 | 83649 | aat gaa ctg atg tcc ttg aaa t | intron 4 | exon 4 | antisense | 38014-38035 | PCR amplification, DNA sequencing | 58 | - | 1185
 | 83032 | cca cat cct act ccg tga c | intron 3 | exon 4 | sense | 37372-37390 | PCR amplification, DNA sequencing | 58 | - | 768
 | 83034 | agg atc ttc ttc tca cca tac | intron 4 | exon 4 | antisense | 38119-38139 | PCR amplification, DNA sequencing | 58 | - | 768
 | 82981 | ctc cgt gac aag [NAME] | intron 3 | exon 4 | sense | 37382-37398 | DNA sequencing | 62 | - | na
 | 82982 | tct aca aag cag gga aat gac | intron 3 | exon 4 | antisense | 37548-37604 | DNA sequencing | 62 | - | na
 | 82955 | tgt ttt gac cct agg ctg | intron 4 | exon 5 | sense | 38266-38283 | PCR amplification, DNA sequencing | 60 | - | 1325
 | 82962 | caa tta ccg tca gat atc cac | intron 5 | exon 5 | antisense | 39566-39586 | PCR amplification, DNA sequencing | 60 | - | 1325
 | 82956 | gag gag cct cag act cat c | intron 4 | exon 5 | sense | 38583-38601 | PCR amplification, DNA sequencing | 58 | - | 792
 | 83035 | gac ata gat ggc tag tcc cta | intron 5 | exon 5 | antisense | 39354-39374 | PCR amplification, DNA sequencing | 58 | - | 792
 | 82957 | ctt cct ctc agc cca tca t | intron 4 | exon 5 | sense | 38635-38653 | DNA sequencing | 62 | - | na
 | 82959 | cag gat cca acc gca tag t | intron 5 | exon 5 | antisense | 39038-39056 | DNA sequencing | 62 | - | na
 | 83670 | [NAME] aaa ata tga tag a | intron 5 | intron 5 | sense | 39257-39278 | PCR amplification, DNA sequencing | 56 | - | 1452
 | 83676 | [NAME] aag [NAME] cat | intron 5 | intron 5 | antisense | 40691-40708 | PCR amplification, DNA sequencing | 56 | - | 1452
 | 86831 | tgg ata tct gac ggt aat tgt ag | intron 5 | intron 5 | sense | 39567-39589 | DNA sequencing | 62 | - | na
 | 83672 | ggt atg ata ata taa ata gtg gat tgt | intron 5 | intron 5 | antisense | 39936-39962 | DNA sequencing | 62 | - | na
 | 83673 | [NAME] tat cag att caa caa t | intron 5 | intron 5 | sense | 40003-40024 | DNA sequencing | 62 | - | na
 | 83674 | ttc caa att ctg ctg tgt atg | intron 5 | intron 5 | antisense | 40241-40261 | DNA sequencing | 62 | - | na
 | 83675 | cac cac cca gag ata atc tca | intron 5 | intron 5 | sense | 40304-40324 | DNA sequencing | 62 | - | na
 | 83039 | gat gcg tcg gtg ata gat | intron 5 | exon 6 | sense | 40437-40454 | PCR amplification, DNA sequencing | 58 | - | 989
 | 83042 | cct aag tcg gct ttt cgt | intron 7 | exon 6 | antisense | 41408-41425 | PCR amplification, DNA sequencing | 58 | - | 989
 | 83661 | [NAME] ttt a | intron 5 | exon 6 | sense | 40589-40607 | PCR amplification, DNA sequencing | 60 | - | 818
 | 83663 | tct ttt gtt tgt tat [NAME] ctc at | intron 7 | exon 6 | antisense | 41384-41406 | PCR amplification, DNA sequencing | 60 | - | 818
 | 83040 | gtt caa ctg cat gag tct tgt g | intron 5 | exon 6 | sense | 40856-40877 | DNA sequencing | 62 | - | na
 | 83041 | [NAME] gag gtg gtg agt | intron 7 | exon 6 | antisense | 41061-41081 | DNA sequencing | 62 | - | na
 | 83045 | tga att cta aat [NAME] g | intron 6 | exon 7 | sense | 53117-53135 | PCR amplification, DNA sequencing | 60 | - | 1058
 | 83048 | cag gca [NAME] tta c | 3' UTR | exon 7 | antisense | 54156-54174 | PCR amplification, DNA sequencing | 60 | - | 1058
 | 82968 | atc tgc ttc tct ctg tgt cag | intron 6 | exon 7 | sense | 53317-53337 | PCR amplification, DNA sequencing | 58 | - | 712
 | 82970 | aga aac tat cca gct att tag g | 3' UTR | exon 7 | antisense | 54007-54028 | PCR amplification, DNA sequencing | 58 | - | 712
 | 83046 | cac ctc cat agc tga gtg acc | intron 6 | exon 7 | sense | 53494-53514 | DNA sequencing | 62 | - | na
 | 83047 | [NAME] tgg gcg tcg | 3' UTR | exon 7 | antisense | 53731-53745 | DNA sequencing | 62 | - | na
 | 83614 | taa taa ctt gcc cta att gg | 3' UTR | 3' UTR | sense | 55986-56005 | PCR amplification, DNA sequencing | 58 | - | 1099
 | 83652 | cgc ttg tca taa ata gtt caa c | 3' UTR | 3' UTR | antisense | 57063-57084 | PCR amplification, DNA sequencing | 58 | - | 1099
 | 83057 | tta gca cat cct aat atg gc | 3' UTR | 3' UTR | sense | 55139-55158 | PCR amplification, DNA sequencing | 58 | - | 938
 | 83060 | cgt tca aaa tgg caa taa c | 3' UTR | 3' UTR | antisense | 56058-56076 | PCR amplification, DNA sequencing | 58 | - | 938
 | 83615 | tgc cat ttt gaa cgt gtt a | 3' UTR | 3' UTR | sense | 56063-56081 | PCR amplification, DNA sequencing | 58 | - | 626
 | 83618 | acc aat ttg att act ccc at | 3' UTR | 3' UTR | antisense | 56669-56688 | PCR amplification, DNA sequencing | 58 | - | 626
 | 83058 | [NAME] ata cc | 3' UTR | 3' UTR | sense | 55432-55451 | DNA sequencing | 62 | - | na
 | 83059 | cca tac taa tgt cat aca tct tgc | 3' UTR | 3' UTR | antisense | 55819-55842 | DNA sequencing | 62 | - | na
 | 83616 | tga aca tag tgg agt gag aag at | 3' UTR | 3' UTR | sense | 56439-56461 | DNA sequencing | 62 | - | na
 | 83617 | cat gat ctt ctg gtt atc gta ag | 3' UTR | 3' UTR | antisense | 56631-56653 | DNA sequencing | 62 | - | na
 | 83618 | acc aat ttg att act ccc at | 3' UTR | 3' UTR | antisense | 56669-56688 | DNA sequencing | 62 | - | na
 | 83626 | cac cga ccc aaa [NAME] | 5' UTR | CDS 1 - 7 | sense | 4688-4704 | RT-PCR Amplification, cDNA sequencing | 58 | - | 1012
 | 82995 | aag gaa [NAME] gta | 3' UTR | CDS 1 - 7 | antisense | 53919-53939 | RT-PCR Amplification, cDNA sequencing | 58 | - | 1012
 | 82993 | [NAME] aag aca cag acc | exon 2 | CDS 1 - 7 | sense | 29282-29302 | cDNA sequencing | 62 | - | na
 | 83776 | [NAME] cac c | exon 5 | CDS 1 - 7 | sense | 38884-38905 | cDNA sequencing | 62 | - | na
 | 83778 | ggt gtg tct tcc tca aaa ata g | exon 5 | CDS 1 - 7 | antisense | 38884-38905 | cDNA sequencing | 62 | - | na
 | 83777 | [NAME] aag tgg atg aac agc a | exon 5 | CDS 1 - 7 | sense | 38934-38955 | cDNA sequencing | 62 | - | na
 | 83779 | tgc tgt tca tcc act tat caa g | exon 5 | CDS 1 - 7 | antisense | 38934-38955 | cDNA sequencing | 62 | - | na
 | 82997 | [NAME] att ca | 3' UTR | CDS 1 - 7 | antisense | 53582-53601 | cDNA sequencing | 62 | - | na
 | 82996 | [NAME] agt aaa ggt gat g | 3' UTR | CDS 1 - 7 | antisense | 53743-53764 | cDNA sequencing | 62 | - | na
 | aUSMARC primer number listed in GenBank Accession number HM355886
 | bPrimer sequences are listed 5' to 3'.
 | cDimethyl sulfoxide was added because the beginning of exon 1 is rich in GC content that is predicted to form strong secondary structures.  The plus or minus symbols indicate the presence or absence of 4% (vol/vol) DMSO in the final cocktail.
 | dNot applicable