Spreadsheet: 1132879b-0ad3-43f8-ba9e-82155dccb7a0
Sheet: Sheet1

Table S3  - 2-DE gel identities from PiPLC-released proteins | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12 | Unnamed: 13
Protein Identity | Clustered ESTs | Accesion number (SchistoDB/NCBInr) | Theorectical pI | Observed | Mr(expt) | Mr(calc) | Delta | Miss | Score | Expect | Peptide sequence | Number of unique peptides | % Coverage
Sm200 (upper spot) |  | Smp_017730** | 4.8 | 1184,6740 | 1183,6667 | 1183,6713 | -0,0046 | 0 | 46 | 0.013 | R.TIHGQIIQFK.L | 4 | 2.94
 |  |  |  | 1344,7894 | 1343,7821 | 1343,7773 | 0,0049 | 0 | 72 | 2.5e-05 | R.ALIITSSGVNTIR.A
 |  |  |  | 1775,8374 | 1774,8301 | 1774,8414 | -0,0112 | 0 | 74 | 1.1e-05 | R.YELQAEVIYTEDFR.D
 |  |  |  | 1470,7813 | 1469,7740 | 1469,7820 | -0,0079 | 0 | 52 | 0.0023 | K.LHVYGLPFHYPK.I
Sm200 (lower spot) |  | Smp_017730** | 4.8 | 1775,8558 | 1774,8485 | 1774,8414 | 0,0072 | 0 | 100 | 2.7e-08 | R.YELQAEVIYTEDFR.D | 1 | 0.84
Hsp70 |  | Smp_106930** | 5.2 | 1081,5742 | 1080,5669 | 1080,5604 | 0,0065 | 0 | 41 | 0.038 | [NAME] | 8 | 9.43
 |  |  |  | 1169,6766 | 1168,6693 | 1168,6564 | 0,0129 | 0 | 54 | 0.002 | K.DAGAIAGLNVLR.I
 |  |  |  | 1228,6439 | 1227,6366 | 1227,6207 | 0,0159 | 0 | 47 | 0.0094 | [NAME]
 |  |  |  | 1253,6307 | 1252,6234 | 1252,6088 | 0,0146 | 0 | 84 | 1.8e-06 | R.FEELNADLFR.G
 |  |  |  | 1473,6967 | 1472,6894 | 1472,6783 | 0,0111 | 0 | 47 | 0.0084 | R.TTPSYVAFTDSER.L
 |  |  |  | 1481,8055 | 1480,7982 | 1480,7998 | -0,0016 | 0 | 52 | 0.0024 | K.SQIHDIVLVGGSTR.I
 |  |  |  | 1691,7316 | 1690,7243 | 1690,7183 | 0,0060 | 0 | 95 | 1e-07 | K.STAGDTHLGGEDFDNR.M
 |  |  |  | 1982,9850 | 1981,9777 | 1981,9745 | 0,0032 | 0 | 99 | 3.1e-08 | R.TVSDAVITVPAYFNDSQR.Q
[NAME] |  | Smp_155890 | 7.3 | 1060,6349 | 1059,6276 | 1059,6077 | 0,0199 | 0 | 52 | 0.0036 | K.GFLLLVEGGR.I | 4 | 10.76
 |  |  |  | 1210,6542 | 1209,6469 | 1209,6354 | 0,0115 | 0 | 68 | 7.3e-05 | K.AGFSTGIVTTTR.V
 |  |  |  | 1290,7040 | 1289,6967 | 1289,6980 | -0,0013 | 0 | 79 | 5.5e-06 | K.GPFNTVGITGTVK.C
 |  |  |  | 1351,7212 | 1350,7139 | 1350,7044 | 0,0095 | 0 | 78 | 6.8e-06 | K.HVLLHTNNEFK.Q
Enolase | Sm12193 | Smp_024110 | 6.5 | 1188,6478 | 1187,6405 | 1187,6298 | 0,0107 | 0 | 77 | 1.1e-05 | K.AGAAEAGLPLYR.Y | 2 | 6.9
 |  |  |  | 1764,9172 | 1763,9099 | 1763,9166 | -0,0067 | 0 | 121 | 2.6e-10 | R.AAVPSGASTGVHEALELR.D
Carbonic Anhydrase | Sm04975 | Smp_168730 | 7.3 | 919,5006 | 918,4933 | 918,4923 | 0,0010 | 0 | 45 | 0.017 | R.EQINLFR.N | 2 | 5.94
 |  |  |  | 1301,6886 | 1300,6813 | 1300,6928 | -0,0115 | 0 | 103 | 2e-08 | R.FPLEGHIVSFR.R
Frutose Biphosphate Aldolase | Sm05684 | Smp_042160.1** | 8.0 | 1296,7334 | 1295,7261 | 1295,7125 | 0,0136 | 0 | 55 | 0.0013 | R.VTEQVLAFVYK.A | 1 | 3.39
Triose Phosphate Isomerase (TPI) | Sm00999 | Smp_003990 | 7.7 | 1737,8911 | 1736,8838 | 1736,8958 | -0,0120 | 0 | 97 | 6.2e-08 | K.VATPQQAQEVHNFLR.K | 1 | 5.92
[NAME] (GST) | Sm02267 | Smp_054160 | 7.0 | 1667,8098 | 1666,8025 | 1666,7951 | 0,0075 | 0 | 92 | 1.8e-07 | K.LIGQAEDVEHEYHK.T | 1 | 6.63
Sm22.6 |  | Smp_045200 | 7.0 | 1038,5414 | 1037,5341 | 1037,5142 | 0,0200 | 0 | 43 | 0.029 | K.SLLDNTYGR.V | 1 | 4.73
Superoxide Dismutase (SOD) |  | Smp_176200.2 | 6.5 | 2213,0789 | 2212,0716 | 2212,0549 | 0,0167 | 0 | 40 | 0.023 | [NAME] | 1 | 13.07
CD59a* | Sm03249 | Smp_019350 | 7.9 | 1362,6088 | 1361,6015 | 1361,6068 | -0,0053 | 0 | 75 | 1.3e-05 | R.SCVPVCVEADAR.R + 2 Carbamidomethyl (C) | 1 | 9.52
CD59a | Sm03249 | Smp_019350 | 7.9 | 1362,6229 | 1361,6156 | 1361,6068 | 0,0088 | 0 | 92 | 2.9e-07 | R.SCVPVCVEADAR.R + 2 Carbamidomethyl (C) | 2 | 23.01
 |  |  |  | 2028,9451 | 2027,9378 | 2027,9405 | -0,0027 | 1 | 99 | 3.5e-08 | R.VKDTEIQNGCTFCSTIR.T + 2 Carbamidomethyl (C)
Thioredoxin |  | Smp_008070 | 5.9 | 1274,6646 | 1273,6573 | 1273,6514 | 0,0059 | 1 | 82 | 2.6e-06 | K.VDVDKLEETAR.K | 2 | 22.63
 |  |  |  | 1511,7321 | 1510,7248 | 1510,7263 | -0,0015 | 0 | 92 | 2.4e-07 | [NAME]
Sm14 | Sm04779 | Smp_095360.1** | 8.3 | 1000,4328 | 999,4255 | 999,4185 | 0,0070 | 0 | 56 | 0.0013 | K.FGEEFDEK.T | 2 | 15.03
 |  |  |  | 1232,6974 | 1231,6901 | 1231,6772 | 0,0129 | 0 | 62 | 0.00029 | [NAME]
[NAME] |  | Smp_089670 | 5.3 | 955,4767 | 954,4694 | 954,4559 | 0,0135 | 0 | 53 | 0.0027 | K.NSFDAVFR.C | 3 | 1.53
 |  |  |  | 1209,6942 | 1208,6869 | 1208,6877 | -0,0008 | 0 | 51 | 0.0041 | R.QVINQIELPR.Y
 |  |  |  | 1386,8016 | 1385,7943 | 1385,8031 | -0,0087 | 0 | 71 | 3.2e-05 | R.FLTLNVNSLLPR.S
Phosphatidylinositol-specific phospholipase C (PiPLC) |  | P08954 | 6.2 | 1255.6405 | [NAME] | 1254.6649 | -0.0316 | 0 | 84 | 5e-05 | K.NYFVDPIFLK.T | 2 | 6.37
 |  |  |  | 1403.7379 | 1402.7306 | 1402.7609 | -0.0302 | 0 | 74 | 0.00047 | K.WSPLLYQEVIR.A
* Isoform associated to less acidic pI. Please refer to Figure 1B
** Peptide hits common to more than one predicted protein