Spreadsheet: 76fe5e4b-b99b-4395-bfff-d70e3be1f407
Sheet: Sheet1

Table S10 | Unnamed: 1 | Unnamed: 2
Primers used in this study.
name | sequence [1] | description
ooEE220 | CACAATTACAACATAATACTGTTGC | GSP1 ORF19.705/Gcn5
ooEE221 | cacggcgcgcctagcagcggCATTACGAATTGTTATTGATCGG | GSP2 ORF19.705/Gcn5
ooEE222 | gtcagcggccgcatccctgcGAATATATGATATGTGATTCTTGGC | GSP3 ORF19.705/Gcn5
ooEE223 | TAACATATTTTCCAACTGAATTGGC | GSP4 ORF19.705/Gcn5
ooEE224 | CTAATTCTGCTCTTATCCATTGTCC | ORF19.705/Gcn5 F
ooEE225 | GTAACCCATTAGCGTATTCTTTAGC | ORF19.705/Gcn5 R
ooEE226 | CGTATGCAGATAACTATGCTATTGG | ORF19.705/Gcn5 int F
ooEE227 | [NAME] | ORF19.705/Gcn5 int R
ooEE15 | CAAGGATGACTACAGCGATATAC | ORF19.3023/NGG1 F
ooEE16 | GTAGGTTGAGTAGAGGAGACATAAC | ORF19.3023/NGG1 R
ooEE28 | GTTTAGATGACTCTTCGATTCTGG | SPT20/ORF19.422 F
ooEE29 | CTGACGGATTAGATTCTGTTTTCC | SPT20/ORF19.422 R
[NAME] | AAGATTAAGTTCGACAAATTGTGCG | GSP1 ORF19.1680 (2nd)
ooEE239 | cacggcgcgcctagcagcggTAAGAAATAAATATTTAGCTTACAAAAAGC | GSP2 ORF19.1680 (2nd)
ooEE254 | gtcagcggccgcatccctgcCATCATGGTCTAGGTCCATTACAAGTGAGC | GSP3 ORF19.1680 (3rd)
ooEE276 | ATTGACAATTTTTTGTTTTGTACATAAGC | GSP4 ORF19.1680 (4th)
ooEE0128 | gtttcgacgagaccaatttcatttATTTCTTAGAAGCTTCGTACGCTGCAGGTC | MSP1/ORF19.1680
ooEE0129 | GTAATAAAATAATCAAAAAGACAAACTACCTtctgatatcatcgatgaattcgag | MSP2/ORF19.1680
ooEE244 | AATAGAAAAGGCTGGTGAGGG | ORF19.1680 F (2nd)
ooEE245 | CATATGATGCTACACCACAACC | ORF19.1680 R (2nd)
ooEE164 | CTGAAATATCCGGTAGAAAAGAACC | ORF19.1680 Internal F
ooEE165 | [NAME] | ORF19.1680 Internal R
ooEE0136 | CTTCTCCTGGTGAAGACTTGTCATTGTTTG | GSP1/ORF19.2598
ooEE0137 | TGTATTAAATGAAATTGGTCTCGTCGAAAC | GSP2/ORF19.2598
[NAME] | TGAGTGTTTCCTTTATTAGTATTACCTCTC | GSP3/ORF19.2598
ooEE0139 | TAAAAGATTGACTAATATTGCACTTGAAGG | GSP4/ORF19.2598
ooEE0140 | gtttcgacgagaccaatttcatttAATACAGAAGCTTCGTACGCTGCAGGTC | MSP1/ORF19.2598
ooEE0141 | GAGAGGTAATACTAATAAAGGAAACACTCAtctgatatcatcgatgaattcgag | MSP2/ORF19.2598
ooEE0156 | TTTCTACTGCTGGGTTTATTAGTGC | ORF19.2598 G1
ooEE0157 | ATTCCCAACTTTGTTGCTACTTCG | ORF19.2598 G2
ooEE162 | CCAAAGACAAGAAGCAGTATAAACC | ORF19.2598 Internal F
ooEE163 | CTGAACCATTGACTACCACAATACC | ORF19.2598 Internal R
oMR1298 | CAACACTCTCAATCAGAAGTGCTTAACAAGTGCAATCCCCGTCCCCAATTATTATCCATAtgtggaattgtgagcggata | GSP1/ORF19.1866
oMR1299 | ATTTTTCCAACATTGTCGCACCGAAACTCTCAAAATGTTCACTGATAAAGCTTAACACACcaacactctcaatcagaagt | GSP2/ORF19.1866
oMR1300 | ATTGTAACCATTTAAATACATTTATTGTAATTGTTGGAGATCGTCTTGTTTGCCTGGCATgttttcccagtcacgacgtt | GSP3/ORF19.1866
[NAME] | CAAACAGAATTATGACTATGAATTACTATCAATTATTTTTTTTTTCTAAACATAACTATAattgtaaccatttaaataca | GSP4/ORF19.1866
ooEE68 | [NAME] | [NAME] R (2nd)
ooEE69 | GGATGTTCAGGTAAGAAGAATGC | [NAME] (2nd)
ooEE48 | TGAAGTCTGCAAAACAAGATGC | [NAME] internal F
ooEE49 | TGGTGTTGGCTTGACAGTAGC | [NAME] internal R
ooEE74 | GGAGTTCCATTTAGAGAAACTCATC | pRS-ARG upstream
ooEE75 | CGAAAATTCTCATATCGGTAGCAAC | pRS-ARG downstream
ooEE76 | GAAGTTGTGTGGGAAAAATCTTCAC | pGEM-HIS upstream
ooEE77 | ACAGTTCACCTGGTACGGTTTTCTAG | pGEM-HIS downstream
ooEE130 | TAGTATAGTGGTCATTATAAATCGTTGTGG | GSP1/ORF19.645.1
ooEE131 | CTATTTATTAAAGAGAGAAATTGAAATTGC | GSP2/ORF19.645.1
ooEE132 | CCGCCATTAGGGTCATGATATGTTACTCAAG | GSP3/ORF19.654.1
ooEE133 | TGGTGGATTTGCTTGTTGGTTCTGATAAAG | GSP4/ORF19.654.1
ooEE134 | [NAME] | MSP1/ORF19.645.1
ooEE135 | CTTGAGTAACATATCATGACCCTAATGGCGGtctgatatcatcgatgaattcgag | MSP2/ORF19.645.1
ooEE154 | CTGTATATCAAAGGTTGTCAAGAGC | [NAME]
ooEE155 | TGAGAGCTAAAGGTGTATCACTTGG | ORF19.645.1 G2
ooEE32 | TGAGAAAGAGAGAGAGAGTTTATCG | ORF19.645.1 F
ooEE33 | AGGAATGTAAGGTAATGACTTGTCG | ORF19.645.1 R
ooEE60 | TATCACAACAAGATCCAAACAAACAAAACTACTCTTTTACACTCCCCTCCCTTTCCCCATCCCCCATCACTGTTGAATCGttatatacttacttgaaatg | ORF19.6590 S1
ooEE61 | GATATTAATCCTGAAGATTATGAAAATGATAGTGATTTATATAGTGATGTTGATGTATAAAAGAAAAAGCCAATATATACATTATTATTATTATTATTAT | ORF19.6590 S2
ooEE38 | AGTGTAACTCCACCAAATGTTACTC | ORF19.6590 F
ooEE39 | AGAAAAGCTAGAGAAGAAGGTAGAG | ORF19.6590 R
oEE108 | AATCGTGAAAATTTCATTAATGG | or19.6590 int F (2nd)
oEE109 | TTTTGTTGACATTTATATAATTCTG | or19.6590 int R (2nd)
oEE256 | CAATCGTGTGAACACAGTAGG | GSP1 ORF19.5237/VPH2
oEE257 | cacggcgcgcctagcagcggTGTATGGGGAAGCTAATGAGG | GSP2 ORF19.5237/VPH2
[NAME] | gtcagcggccgcatccctgcAAATTTGAAATTGGATTAGTTCGTGGG | GSP3 ORF19.5237/VPH2
oEE259 | CGACTTAGAAAGTTAATGCTTCTTTGC | GSP4 ORF19.5237/VPH2
oEE260 | TTTCTCGAGTGAATCTCTGCC | ORF19.5237/VPH2 F
oEE261 | [NAME] | ORF19.5237/VPH2 R
oEE262 | CATGTCACCCAACGTCTACC | ORF19.5237/VPH2 int F
oEE263 | TTCAATTCTTTCAGTGCTTGTTGG | ORF19.5237/VPH2 int R
oMR1302 | TTTTGATTTTTATTATTAATACCTAACAAATATATCAAACTAATATTTTAATCAACCATCtgtggaattgtgagcggata | GSP1/ORF19.6573
oMR1303 | GTTCTTTATAAATCTATTGGGATCTTACAAACATCATATCCATTTTTTTATATTTTTGATttttgatttttattattaat | GSP2/ORF19.6573
oMR1304 | TATTATCATTATCACTTTCTATAAATACAAAAGTGAGCTTTAGAAAATCACATTTTATATGttttcccagtcacgacgtt | GSP3/ORF19.6573
oMR1305 | TTGTAAAACATTTCTAAAAAATATTCGTATATATATATATATATATGTATGTATAAATTAtattatcattatcactttct | GSP4/ORF19.6573
oEE34 | GTGTTTCTCACGATACTAATTCACC | BEM2/ORF19.6573 F
oEE35 | CACCACTACCACTATTTCCTGTAGC | BEM2/ORF19.6573 R
oEE62 | AAATCCAAAAAAATCTTGTCATTCGATTCAACGATATTCCTTTTTAGATTTGAATTACTTTATAGTATAAAATAATTGATcctttatccttcttatcatg | ORF19.3683 S1
oEE63 | ATAAATATGTATAAATATATATCATATATATCATATCTATATATAATCTATACATAAATGATCTAAAAGACAAAAAGAAGTactagactaattgaggtta | ORF19.3683 S2
oEE26 | GGCAAGTATCTTCTGGTGTTGG | AGE3/ORF19.3683 F
oEE27 | GAAACGAAGCATTATTGTCAGC | AGE3/ORF19.3683 R
oEE40 | GTGCTGGAGTACATAGAGGTTTAGG | AGE3/ORF19.3683 int F
oEE41 | CATTTGTTAATGGAACAGGAGTAGG | AGE3/ORF19.3683 int R
oEE269 | CATTACATTATTATGGTGTACCATTCC | AGE3/ORF19.3683 F (2nd)
oEE270 | CAATGATGTTAGATTATAATCCAGTTCC | AGE3/ORF19.3683 R (2nd)
oEE237 | [NAME] | AGE3 Not1
oEE238 | TCCCCGCGGGGACAGCTCATTCTCAATTCATGGG | AGE3 SacII
oEE242 | CGGGGTACCCCGGTATTAGATCAAATGCTATTGCCCC | AGE3 Kpn1 2nd
oEE243 | CCGCTCGAGCGGGACTTGTTGAATGGATTTTAAATTGGCC | AGE3 Xho1 2nd
oEE30 | GAATGGTAAGCTAAGTGAGTTGTC | SAC6/ORF19.5544 F
oEE31 | AGTATAGTTTTCCCCATCACTGAC | SAC6/ORF19.5544 R
oMR1091 | CCACCACCCAAGTACACATTT | ORF19.736 F
oMR1092 | TGACATTGGACCCTGATTGA | ORF19.736 R
oEE21 | GTTGAAGAGGGACTGGTAGTTATA | ORF19.794 F
oEE22 | ATCTCCAATAATCGGTTGTG | ORF19.794 R
oEE13 | CCATAACAGCAACAAAGACTTC | ORF19.1298 F
oEE14 | GGTTTTGGATCAAGGTAATG | ORF19.1298 R
oEE1 | GAACAGTAATAGTAAGACGAGTAGC | ORF19.6000 F
oEE2 | ACCAGTAGTGACTCTTTCAGAT | ORF19.6000 R
oMR1095 | TCGGTTGATGGTTTTTGTC | ORF19.923 F
oMR1096 | GCGTTAAACTAGCCTAACTT | ORF19.923 R
oMR1089 | GCAATCGACCCTTCCATAAATA | ORF19.3038 F
oMR1090 | CTTGGCACCATTAGTAGGA | ORF19.3038 R
oEE235 | CGGGGTACCCCGGGCAAGTATCTTCTGGTGTTGG | AGE3 Kpn1
oEE236 | CCGCTCGAGCGGGAAACGAAGCATTATTGTCAGC | AGE3 Xho1
[1] Lower-case letters (in blue) indicate annealing sequences for auxotrophic marker amplification or, as in the case for Gene Specific Primers (GSP), annealing sequences for fusion PCR (Noble et al. Strains and Strategies for Large-Scale Gene Deletion Studies of the Diploid Human [NAME] albicans. Eukaryotic [NAME] (2005). Underlined sequences indicate restriction recognition sites. PCR verification of mutant constructs was done as previously described ([NAME]. New pFA-cassettes for PCR-based gene manipulation in Candida albicans. J Basic Microbiol (2006).