Spreadsheet: 0392727c-041f-46cf-ba63-facdc9c6a6b4
Sheet: Sheet1

Additional File 10. Construction of promoter fusions for selected transcription-factor genes. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5
AGI | 5' flanking region (bp) 1 | Forward primer (5'-3') 2 | Reverse primer (5'-3') 2 | Cloning method | Vector
AT1G06160 | -2304 to +15 | AAAAGGGGTACCCACTGTCGCTGAAACGTCC | CTCTCTCGCGGATCCAGTTTGATATTCCATTTTCGATC | KpnI / BamHI | pBI-n1GFP
AT1G28160 | -1982 to +33 | GTCAGTCCCAAGCTTCGACTTACGGTGTATTCCATG | GAGAGCTCTAGAAGAACCGGCATTCAAATTACCA | HindIII / XbaI | pBI-n1GFP
AT1G35520 | -1683 to +39 | CATGCAGTGAAGCTTCTCATCAAACCAATTGACCC | CCTCTGAGCTCTAGATAATTCAGGTTGTGCATTCAC | HindIII / XbaI | pBI-n1GFP
AT1G43160 | -2113 to +36 | [NAME] | GACTCGGGATCCCTCACCAGAGACAACATTAGT | HindIII / BamHI | pBI-n1GFP
[NAME] | -2009 to +18 | TGATTACGCCAAGCTTGTGTTACGTTGTAACGAATTT | TGCTCACCATGGATCCCTCTTGAGCATTAGTCATATTG | HindIII / BamHI | pBI-GFP[S65T]
AT1G55600 | -2050 to +9 | TGATTACGCCAAGCTTAATGTAAGAGTTTACTAAGC | TGCTCACCATGGATCCATCACTCATTTTTGACAAATC | HindIII / BamHI | pBI-n1GFP
AT1G60280 | -1317 to +15 | TGATTACGCCAAGCTTCGTCGTCGCCATGTATAAAC | TGCTCACCATGGATCCGTCTTCAACTTTCATTGGTTCG | HindIII / BamHI | pBI-n1GFP
AT1G67030 | -2658 to +12 | AAACGGGGTACCGTACTAATACGAGAATTGGGCA | TCAGGCTCTAGATTCAGTCGCCATATATGTGAGC | KpnI / XbaI | pBI-n2GFP
AT1G75250 | -2018 to +18 | TGATTACGCCCTGCAGTATAAAAAAACACATGGAGA | TGCTCACCATGGATCCTCTTGAGTTTGACGCCATTAAT | PstI 3/ BamHI | pBI-GFP[S65T]
AT1G75490 | -1434 to +15 | TATTCACGCGTCGACTGCTGTGTCACCTGGATTGC | TGCTCACCATGGATCCCTCTATGGATGACATGTACGGATG | SalI / BamHI | pBI-n1GFP
AT2G22750 | -1921 to +18 | TGATTACGCCTGCAGCCCTAGCCAATCACATTC | TGCTCACCATTCTAGATCCGACGAGTGAGTTCATGT | PstI 3/ XbaI | pBI-GFP[S65T]
AT2G33710 | -1976 to +36 | GACTGAGAGAAGCTTCTCCGTAACTCAATCTCAGC | GTCAGTCGGGATCCTGATTCTGGTGATAGAGGTCT | HindIII / BamHI | pBI-n1GFP
AT2G40220 | -2557 to +15 | ATATGGGGTACCGGCAATTGGGTTGAGATCTC | GAGACGGGATCCAGCTAAAGGGTCCATAGATG | KpnI / BamHI | pBI-n1GFP
AT3G01030 | -896 to +15 | ATATGGGGTACCGCAAGCAAAGACTAATGCTG | ATATCGGGATCCGCCAGTCTTCTTCATGAGAT | KpnI / BamHI | pBI-n2GFP
AT5G01380 | -1318 to +9 | TGCTCATCCGCTCGAGCCATTTCAGAACAAGAGTAC | TGCTCACCATGGATCCTCGGTCCATTATTTGGAATCG | XhoI / BamHI | pBI-n1GFP
AT5G01860 | -1453 to +18 | AAACGGGGTACCTTAGGTACAAGAAGGGGTCTTC | AAACGCGGATCCAGAGAGGTTTAGTTCCATAGTC | KpnI / BamHI | pBI-1GFPB, pBI-n1GFP, pBI-n2GFP 4
AT5G11050 | -1487 to +10 | TGATTACGCCAAGCTTTGCTCTGTTAGTTGATTTC | TGCTCACCATGGATCGTTCTTCCATGGAAGAAAAAG | In-Fusion | pBI-GFP[S65T]
AT5G15830 | -1955 to +39 | CTTACTCCGCTCGAGGACCAACTAAGAGATAACACTCG | GACTCGGGATCCCATGTTGTTAAGACTTGAGCTATC | XhoI / BamHI | pBI-n1GFP
AT5G27880 | -2059 to +12 | AAACGGGGTACCCTGAATGTGCAAACAAACATGTC | AAACGCGGATCCTGAAGAAGACATAGCTGGTTCTC | KpnI / BamHI | pBI-1GFPB, pBI-n1GFP, pBI-n2GFP 4
AT5G41090 | -1960 to +15 | CTGAAGGGGTACCTCAAGCTTCTCTCTCAGTTG | AAAACGGGATCCGTATGTTGGGCCCATTATTC | KpnI / BamHI | pBI-n2GFP
AT5G45980 | -1370 to +12 | TGATTACGCCAAGCTTTCAAACAAGAAAGATGCTTGG | TGCTCACCATGGATCCTGAGGAGGACATGATGATGG | HindIII / BamHI | pBI-n1GFP
AT5G50490 | -960 to +18 | GAGTGACCCAAGCTTTGAAGTGCATTGGTTCGA | GTGTCGGGATCCGTTGTTGTTGTTCTCCATTTT | HindIII / BamHI | pBI-n1GFP
[NAME] | -1245 to +15 | TGATTACGCCAAGCTTTCTTGAAACTTCGTGTAGC | TGCTCACCATGGATCCCTGAGAATTCTCCATTTTTCTC | HindIII / BamHI | pBI-n1GFP
AT5G56200 | -2167 to +18 | CACACGGAAAAGCTTGTGGCCCTCCACTACTAAG | GACTCGGGATCCACCTCGTAATTGGTCCATCG | HindIII / BamHI | pBI-n1GFP
The 5' upstream sequences of 24 transcription-factor genes were amplified using the forward and reverse primer, and cloned into the designated vectors through restriction digestion or In-Fusion technology (BD Biosciences) as described in Methods. The AGI numbers are listed in ascending order.    1 Positions are relative to the predicted translation start codon.     2 Underlined sequences are restriction sites or vector sequences for homologous recombination through In-Fusion technology.    3 The PCR product was digested with PstI, while the binary vector was digested with SbfI (compatible with PstI).     4 The amplified PCR product was first cloned into pBI-n2GFP, sequenced, then subcloned into pBI-n1GFP or pBI-1GFPB.