Spreadsheet: 393dd017-5dd1-44f0-86c4-8e4da91074ec
Sheet: Sheet1

Supplementary Table S5: Selected Regions for Sanger Sequencing, [NAME] and Primer Details | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11
region_start | region_end | name_of_chunk | [NAME] | position | sequence (in forward orientation) | Tm | name_of_reverse_primer | position | sequence (will amplify in reverse orientation) | Tm | Estimated product_length
1 | 500 | CH1 | CH1-F | 5-26 | CGCGTTTCCCGAGAAGGTCTGG | 60 | CH1-R | 527-546 | TGCGGTTCACCGACAGCACA | 59 | 542
49933 | 50432 | CH4 | CH4-F | 49843-49862 | GGCGTCTTACGGGCGCAACT | 60 | CH4-R | 50437-50456 | GCAGGAGAATCTGCCGGCCC | 60 | 614
210190 | 210689 | CH7 | CH7-F | 210027-210046 | GAACGGCCGGCGTGTTCTGA | 60 | CH7-R | 210665-210684 | CCCCATGAATGCGCCCCAGG | 60 | 658
210690 | 211190 | CH8 | [NAME] | 210665-210684 | CCTGGGGCGCATTCATGGGG | 60 | CH8-R | 211140-211159 | CGACGTGACCGAGATCGGCG | 60 | 495
234789 | 235288 | CH9 | CH9-F | 234806-234825 | GGTTTCGGCCGCGTCCTTCA | 60 | CH9-R | 235332-235351 | ATCCGCCTGGCTCGGGAACT | 60 | 546
235289 | 235789 | CH10 | CH10-F | 235332-235351 | AGTTCCCGAGCCAGGCGGAT | 60 | CH10-R | 235770-235789 | GGCGAAGGGGGAGCCAACAC | 60 | 458
434644 | 435143 | CH11 | CH11-F | 434586-434605 | [NAME] | 60 | CH11-R | 435174-435193 | TGTCCCGACGGGGTTTGGGT | 60 | 608
435144 | 435644 | CH12 | CH12-F | 435042-435061 | [NAME] | 60 | CH12-R | 435608-435627 | AGGCGATCCCCTGGCTGGAG | 60 | 586
474322 | 474821 | CH13 | CH13-F | 474109-474128 | CTCCTCCGGCTCGGCTCTGT | 60 | CH13-R | 474751-474770 | TCAGGAACAGGCGCCGCAAG | 60 | 662
474822 | 475322 | CH14 | [NAME] | 474797-474816 | AGTTCCACGGCCAGGTTGCG | 60 | [NAME] | 475449-475468 | CCTGGCCACCATGTACGCCG | 60 | 672
795396 | 795895 | CH15 | [NAME] | 795402-795421 | CGGCTTTTCGGGCAGGTCGT | 60 | CH15-R | 795821-795841 | CCCAGGTGTTGCGAGCCTTCA | 59 | 440
795896 | 796396 | CH16 | CH16-F | 795813-795832 | [NAME] | 59 | CH16-R | 796424-796443 | CGACCTTGGGATCGTGGGCG | 60 | 631
864896 | 865395 | CH17 | CH17-F | 864675-864694 | CCTGCTGGGGGTGACCCTGT | 60 | CH17-R | 865327-865346 | GGCGACCACGATCACGCCAT | 60 | 672
865396 | 865896 | [NAME] | [NAME] | 865407-865426 | [NAME] | 60 | [NAME] | 865915-865934 | GACGATGGCGGATTGCCCCC | 60 | 528
943735 | 944234 | CH19 | CH19-F | 943758-943777 | CCCCTCTCGCTCCAGCAGGT | 60 | CH19-R | 944362-944381 | GATGGGGCGATCCGACGCTG | 60 | 624
944235 | 944735 | CH20 | [NAME] | 944197-944216 | CGCAACCGCGCCACATGTTC | 60 | [NAME] | 944804-944823 | CGACGGCCACGAAGCCCTTT | 60 | 627
1252207 | 1252706 | CH21 | [NAME] | 1252143-1252162 | TCGCCGCGCAGATGTCTTGG | 60 | CH21-R | 1252649-1252668 | CGCCCGACACACATCGGGAC | 60 | 526
1252707 | 1253207 | CH22 | [NAME] | 1252628-1252647 | TTTTCCCGTGAGGTCGCCGC | 60 | CH22-R | 1253303-1253322 | ACGGCGGCAATCATGCCGAT | 60 | 695
1453524 | 1454023 | CH23 | [NAME] | 1453579-1453598 | ATCGGGCCAGACGCTCCTGT | 60 | CH23-R | 1454224-1454243 | TTGCCTCTGACGTGCCTGGA | 58 | 665
1454024 | 1454524 | CH24 | CH24-F | 1453942-1453962 | AGGCCTTCAGCAATGGGCCTC | 59 | CH24-R | 1454492-1454512 | CGCGAGCTGGACGACCATCAA | 60 | 571
1471223 | 1471722 | CH25 | CH25-F | 1471128-1471147 | GTCCTGTCCCGCGACTCCCT | 60 | CH25-R | 1471674-1471693 | GATGCTCAGGTGGCGGTCGG | 60 | 566
1471723 | 1472223 | CH26 | [NAME] | 1471611-1471630 | TCGCCAAGCACGGGCTCAAG | 60 | CH26-R | 1472261-1472280 | GGGAGAGTGCGCTTCCGCTC | 60 | 670
1578218 | 1578717 | CH27 | CH27-F | 1578122-1578141 | CCTGCTCGCAAAGTCGGCCA | 60 | CH27-R | 1578692-1578711 | TCATGCCGGGCTTACGGGGA | 60 | 590
1578718 | 1579218 | CH28 | CH28-F | 1578692-1578711 | TCCCCGTAAGCCCGGCATGA | 60 | CH28-R | 1579157-1579176 | CGCTGGACGCAGGGCTGTAG | 60 | 485
1593199 | 1593698 | CH29 | CH29-F | 1593250-1593269 | GCGCGATAAGGCGGTCGGAA | 60 | CH29-R | 1593916-1593935 | CCTGCACTGCCAAGCGGACA | 60 | 686
1593699 | 1594199 | CH30 | [NAME] | 1593721-1593740 | AGGCTGAACTGCGGAGGGGT | 60 | CH30-R | 1594354-1594373 | TCGTCCCCCGCCAACGGTAT | 60 | 653
1611551 | 1612050 | CH31 | [NAME] | 1611467-1611486 | TGCTCCGTGCTCGGCAATCG | 60 | CH31-R | 1612080-1612099 | TGACGCCGCGCTGTTGTACG | 61 | 633
1612051 | 1612551 | CH32 | CH32-F | 1612080-1612099 | CGTACAACAGCGCGGCGTCA | 61 | CH32-R | 1612512-1612531 | GTCACCGGTTCCCAAGGCGG | 60 | 452
1855569 | 1856068 | CH33 | CH33-F | 1855610-1855629 | CATGAGCGCCGTAGTGGCCC | 60 | CH33-R | 1856260-1856279 | CCCCCAGGGCCGAAGATGGA | 60 | 670
1856069 | 1856569 | CH34 | [NAME] | 1855941-1855960 | GCCGGCAAAAGGGCGAAAGC | 60 | CH34-R | 1856497-1856516 | AGGGCGGTCCTTTCCCGAGG | 60 | 576
1890003 | 1890502 | CH35 | [NAME] | 1889868-1889887 | TCCCTGAGACAGCCGGGTCG | 60 | CH35-R | 1890515-1890534 | TTGAGTTGGGTGCGCAGGGC | 60 | 667
1890503 | 1891003 | CH36 | CH36-F | 1890452-1890471 | TATGCATCCCCCTCCCCCGC | 60 | CH36-R | 1891042-1891061 | TCCCGTAGAGTGCCGACCCG | 60 | 610
1998425 | 1998924 | CH37 | [NAME] | 1998206-1998228 | TCTTCTAAATGGGGACAGCGCCA | 58 | CH37-R | 1998870-1998891 | TCAGTCGCGGTTAGCATGTCTG | 57 | 686
1998925 | 1999425 | CH38 | CH38-F | 1998806-1998825 | CCTGCCTGAGCCATCCTCGG | 59 | CH38-R | 1999442-1999465 | TGGAATGAGTATCGGCTACGCAAC | 57 | 660
2123076 | 2123576 | CH40 | [NAME] | 2122849-2122868 | CGTTGTCGCTGGCCTCCACT | 59 | CH40-R | 2123513-2123532 | TTTGAAGGCCGGACGCGAGC | 60 | 684
2284365 | 2284864 | CH43 | CH43-F | 2284354-2284373 | GGCCGGACAGATGCGGCTAC | 60 | CH43-R | 2284850-2284869 | AAACCACCTGGGGCCGGAGT | 60 | 516
2284865 | 2285365 | CH44 | [NAME] | 2284850-2284869 | ACTCCGGCCCCAGGTGGTTT | 60 | CH44-R | 2285340-2285359 | TCTGTTGCCGCTGGTGCAGG | 60 | 510
2524407 | 2524906 | CH45 | CH45-F | 2524394-2524416 | TCGCTTCCCGAATTACCACAACG | 58 | CH45-R | 2525070-2525090 | CGGCATCAAGAGGGTCTGGCA | 59 | 697
2524907 | 2525407 | CH46 | [NAME] | 2524865-2524887 | GCACACGTTTTGCACCCTACCCT | 60 | CH46-R | 2525518-2525537 | AACTGCTGCCACGCCCTACG | 60 | 673
2630039 | 2630538 | CH47 | [NAME] | 2630035-2630054 | TTCAAAGGCCTGCCGGAGCG | 60 | CH47-R | 2630572-2630591 | GCCCCGGCCATGAAACCTCC | 60 | 557
2630539 | 2631039 | CH48 | CH48-F | 2630370-2630389 | GGGGCCGTCCTCGATCACCA | 60 | CH48-R | 2631035-2631054 | CTGATGCACACGGCCTCCCG | 60 | 685
2658071 | 2658570 | CH49 | CH49-F | 2657949-2657968 | GGCATGTCGGCTTCTGCGGT | 60 | CH49-R | 2658594-2658613 | CGACGCCACCAGCAACGTGA | 60 | 665
2751194 | 2751693 | CH51 | [NAME] | 2751055-2751074 | CCACGAGATGGCAGTGGCGG | 60 | CH51-R | 2751656-2751675 | CGTGCTGAGCAGTGGGACCG | 60 | 621
2851969 | 2852468 | CH53 | [NAME] | 2851792-2851811 | GGCCGTTGCGAACGATTGCC | 60 | CH53-R | 2852460-2852479 | GGCAGGGCGGGTTCTTTCCC | 60 | 688
2855121 | 2855620 | CH55 | CH55-F | 2855134-2855153 | AGGCCGATCAGGACGGGGAC | 60 | CH55-R | 2855793-2855812 | TGCAGGGGATGCACGCACAG | 60 | 679
2855621 | 2856121 | CH56 | [NAME] | 2855425-2855444 | AGAAGCGCGGCTGCAGTGAG | 60 | CH56-R | 2856083-2856102 | CGGCATCAGCCTGGACGTGG | 60 | 678
2902542 | 2903041 | CH57 | [NAME] | 2902515-2902534 | CCGCGATTACGGACGGGTCG | 60 | CH57-R | 2903026-2903045 | GTGACCAAGGACGGCACGGG | 60 | 531
2903042 | 2903542 | [NAME] | [NAME] | 2903026-2903045 | CCCGTGCCGTCCTTGGTCAC | 60 | [NAME] | [PHONE] | AGCGCTGCCACACTTCGACC | 60 | 586
2982484 | 2982983 | CH59 | [NAME] | 2982487-2982506 | AATTCCGGCGCACCCGTTGT | 60 | CH59-R | 2983010-2983029 | GCGGTAGGAAGCCGGGATGC | 60 | 543
2982984 | 2983484 | CH60 | CH60-F | 2982894-2982913 | CCACGTCGTCAAGCGGGACC | 60 | CH60-R | 2983465-2983484 | TGGGCGTCGCCGTAGGAGAA | 60 | 591
2987493 | 2987993 | CH62 | CH62-F | 2987372-2987391 | CCGCCATGGGCGAGCATGAA | 60 | CH62-R | 2988041-2988060 | GCCGGTACCCGGTGAGGTCT | 60 | 689
3199426 | 3199925 | CH63 | [NAME] | 3199346-3199365 | TCCGTCGCTCCCCTCTTCCC | 60 | CH63-R | 3199863-3199882 | GGGCGGCTTACCACGCAAGT | 60 | 537
3199926 | 3200426 | CH64 | [NAME] | 3199863-3199882 | ACTTGCGTGGTAAGCCGCCC | 60 | CH63-R | 3200449-3200468 | TGGCGACCTTATCGCGGGGA | 60 | 606
3207925 | 3208424 | CH65 | CH65F | 3207800-3207819 | ATTGTCGCCGAGCACCGCAT | 60 | CH65-R | 3208441-3208460 | GATCCGGCTCGCTTCCCGTG | 60 | 661
3208425 | 3208925 | CH66 | CH66-F | 3208433-3208452 | CACAGTGGCACGGGAAGCGA | 60 | CH66-R | 3209089-3209109 | TGCCAACCCAGGGGAAAAGCA | 59 | 677
3316457 | 3316956 | CH67 | CH67-F | 3316484-3316503 | CACGGCGGGCTTCTTGTCGT | 60 | CH67-R | 3316976-3316995 | CACCCAACGTCGGCTGGTCC | 60 | 512
3316957 | 3317457 | CH68 | [NAME] | 3316982-3317001 | GCCGACGTTGGGTGCCAGAA | 60 | CH68-R | 3317388-3317407 | CCCATGCCCAACGGCCACAT | 60 | 426
3372431 | 3372930 | CH69 | [NAME] | 3372385-3372404 | AACTCCCCCGCGAGGAGACC | 60 | CH69-R | 3372863-3372882 | CAGACGCCAGACATCCCGGC | 60 | 498
3372931 | 3373431 | CH70 | [NAME] | 3372948-3372967 | ATCAGCCTCGTCGCTTCGGC | 60 | CH70-R | 3373555-3373574 | TCGGGATCACCTCCCGCTCG | 60 | 627
3477191 | 3477690 | CH71 | CH71-F | 3477245-3477264 | CCGGTGCGTCCCCACCATTG | 60 | CH71-R | 3477747-3477766 | GGTGAAGTGGCCCGGCTGTC | 60 | 522
3477691 | 3478191 | CH72 | [NAME] | 3477743-3477762 | TCCTGACAGCCGGGCCACTT | 60 | CH72-R | 3478260-3478279 | TTTTCACTGCGCGACGCCCT | 60 | 537
3485342 | 3485841 | CH73 | CH73-F | 3485276-3485295 | GGCGAGAATGATGGCGGCGA | 60 | CH73-R | 3485792-3485811 | CACCGGCTCTTGCACCCCTG | 60 | 536
3485842 | 3486342 | CH74 | [NAME] | 3485792-3485811 | CAGGGGTGCAAGAGCCGGTG | 60 | CH74-R | 3486415-3486434 | CGCCGGGGTCGGATTTGTCC | 60 | 643
3490774 | 3491273 | [NAME] | [NAME] | 3490714-3490773 | GCCTGACACCATGCCGCACT | 60 | [NAME] | 3491296-3491315 | CCGGGGCTCCGACTGCAAAA | 60 | 602
3491274 | 3491774 | CH76 | CH76-F | 3491150-3491169 | AGGTCGCCTCGCCACCTCAT | 60 | CH76-R | 3491828-3491847 | GTGGCGGCCTCCTCCGTTTC | 60 | 698
3583497 | 3583996 | CH77 | [NAME] | 3583448-3583467 | ACCATCGCCACCACCGAGGA | 60 | CH77-R | 3584090-3584109 | GACCGGGAGTTTACCGCCGC | 60 | 662
3583997 | 3584497 | CH78 | CH78-F | 3583824-3583843 | CTGCCCATCCCGAGCCTCCT | 60 | CH78-R | 3584479-3584498 | CGGCAGCCCATATCTCGCGG | 60 | 675
3685210 | 3685709 | CH79 | CH79-F | 3685161-3685180 | GAGACGGGGTGAGCCGACCA | 60 | CH79-R | 3685687-3685706 | CTGGGGCCGCACATTCAGCA | 60 | 546
3685710 | 3686210 | CH80 | CH80-F | 3685648-3685667 | CGGACGCACCGGTAGCACTG | 60 | CH80-R | 3686208-3686227 | CGCTCCTGCTAGGCATCGGC | 60 | 580