Spreadsheet: cf193d92-b826-4457-b745-64ca3528090f
Sheet: Ung+

mutant number | mutated gene | position in Chr. V (distance from telomere) | distance between adjacent mutations | >1 mutation in gene? | WT base(s) | mutant base(s) | deletion/ insertion (-/+ number of bases) | 5′ flanking sequence | 3′ flanking sequence | type of mutation
066-051-G12A | ADE2 | 3849 | 670 | + | G | C |  | GTTACCCAAAGTGTTCCTGT | GAACAAGCCAGTGAGACGTC | substitution
 | ADE2 | 4519 | 3464 | + | G | T |  | GTTCCTCAGTGTACTTATAT | GAAAAGAGTCTAGACCTAAC | substitution
 | CAN1 | 7983 |  |  | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
066-051-G12B | ADE2 | 4025 | 968 | + | G | T |  | [NAME] | [NAME] | substitution
 | ADE2 | 4993 | 2880 | + | G | C |  | CAATTGTGCAAATGCCTAGA | GTGTTCCAGTAGCTACCGTC | substitution
 | CAN1 | 7873 |  |  | G | C |  | TATTCTGTCACGCAGTCCTT | GGCGAAATGGCTACATTCAT | substitution
066-051-G13A | ADE2 | 3894 | 131 | + | G | C |  | TTGAATGTTGGAAGAGATTT | GGTTTTCCATTCGTCTTGAA | substitution
 | ADE2 | 4025 | 4039 | + | G | A |  | [NAME] | [NAME] | substitution
 | CAN1 | 8064 |  |  | G | A |  | CAAAGTTCCACTGGCGGCAT | GATTAGTATTTTTTGGGTAA | substitution
066-051-G15 | ADE2 | 4261 | 2268 |  | G | - | -1 | TGTTCTATTTAGAAACAGGG | AATTGCTTATTAACGAAATT | deletion
 | URA3 | 6529 | 1642 |  | G | A |  | GCAGTTGGGTTAAGAATACT | GGCAATTTCATGTTTCTTCA | substitution
 | CAN1 | 8171 |  |  | G | C |  | TCAAAGTTTTAGCCATTATC | GGTTTCTAATATACTGTTTT | substitution
066-051-G16A | 3′ LYS2 | 2342 | 1961 |  | G | A |  | TATATATACCAACCTCTTCT | GAGTAACACCTATTCCATTA | substitution
 | ADE2 | 4303 | 3680 |  | G | C |  | CCCCAAGGCCTCACAACTCT | GACATTATACCATTGATGCT | substitution
 | CAN1 | 7983 | 1131 | + | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
 | CAN1 | 9114 |  | + | G | A |  | ATTCAGATGCAGATTTATTT | GAAGATTGGAGATGTCGACA | substitution
067-051-H15 | ADE2 | 3883 | 348 | + | G | C |  | AGACGTCCCTATTGAATGTT | GAAGAGATTTGGGTTTTCCA | substitution
 | ADE2 | 4231 | 3848 | + | G | C |  | TTCCCGGTTGTGGTATATTT | GTGTGGAAATGTTCTATTTA | substitution
 | CAN1 | 8079 | 92 | + | G | C |  | GGCATGGATTAGTATTTTTT | GGTAATTATCACAATAATGA | substitution
 | CAN1 | 8171 |  | + | G | A |  | TCAAAGTTTTAGCCATTATC | GGTTTCTAATATACTGTTTT | substitution
067-060-A1A | ADE2 | 4698 | 2864 |  | G | A |  | CCATTGGTTGGAATCATCAT | GGATCAGACTCTGACTTGCC | substitution
 | CAN1 | 7562 | 609 | + | G | A |  | CACAAACACACCACAGACGT | GGTCAATACCATTAAAAGAT | substitution
 | CAN1 | 8171 |  | + | G | A |  | TCAAAGTTTTAGCCATTATC | GGTTTCTAATATACTGTTTT | substitution
067-060-A1B | ADE2 | 3895 | 4088 |  | -G | CT | +1 | TGAATGTTGGAAGAGATTTG | TTTTCCATTCGTCTTGAAGT | complex
 | CAN1 | 7983 |  |  | G | - | -1 | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | deletion
067-060-A3 | ADE2 | 3937 | 1931 |  | G | C |  | CGAGGACTTTGGCATACGAT | GAAGAGGTAACTTCGTTGTA | substitution
 | URA3 | 5868 | 2303 |  | G | A |  | TTGTCATCTAAACCCACACC | GGTGTCATAATCAACCAATC | substitution
 | CAN1 | 8171 | 139 | + | G | A |  | TCAAAGTTTTAGCCATTATC | GGTTTCTAATATACTGTTTT | substitution
 | CAN1 | 8310 |  | + | G | A |  | CGAAGGGAGGTTCTTAGGTT | GGTTTCCTCTTTGATTAACG | substitution
067-060-A4A | ADE2 | 4871 | 3090 |  | [NAME] | ---CG | -3 | AATTATCGCTGGAGCTGGTG | GCTCACTTGCCAGGTATGGT | complex
 | CAN1 | 7961 |  |  | G | C |  | CAGCATTTGGTGCGGCCAAT | GTTACATGTATTGGTTTTCT | substitution
067-060-A4B | ADE2 | 3894 | 4089 |  | - | C |  | TTGAATGTTGGAAGAGATTT | GGGTTTTCCATTCGTCTTGA | insertion
 | CAN1 | 7983 |  |  | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
067-060-A4C | ADE2 | 4588 | 3395 |  | G | - | -1 | CCTCCAGTATGGCGGAATGT | AACAAAGGCTGAACTACATT | deletion
 | CAN1 | 7983 |  |  | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
067-064-A1A | 3′ LYS2 | 2615 | 1410 |  | G | C |  | AGTCTTTGAATAATATTCTT | GGTGACCCGTTACTTGAGCA | substitution
 | ADE2 | 4025 | 2290 |  | G | A |  | [NAME] | [NAME] | substitution
 | URA3 | 6315 | 1525 |  | G | C |  | GTTTTTAGTAAACAAATTTT | GGACCTAATGCTTCAACTAA | substitution
 | CAN1 | 7840 | 143 | + | G | C |  | CTTATATCATATTTATTTAT | GGTTCTTTGGCATATTCTGT | substitution
 | CAN1 | 7983 | 188 | + | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
 | CAN1 | 8171 | 943 | + | G | A |  | TCAAAGTTTTAGCCATTATC | GGTTTCTAATATACTGTTTT | substitution
 | CAN1 | 9114 |  | + | G | C |  | ATTCAGATGCAGATTTATTT | GAAGATTGGAGATGTCGACA | substitution
067-064-A4A | 3′ LYS2 | 2751 | 1552 |  | G | C |  | ATCTGGAATCTTACCTAATT | GACTGAACCTTTCAAAAATC | substitution
 | ADE2 | 4303 | 3776 |  | G | A |  | CCCCAAGGCCTCACAACTCT | GACATTATACCATTGATGCT | substitution
 | CAN1 | 8079 | 215 | + | G | A |  | GGCATGGATTAGTATTTTTT | GGTAATTATCACAATAATGA | substitution
 | CAN1 | 8294 | 258 | + | G | C |  | TATCTAAGGATAAAAACGAA | GGAGGTTCTTAGGTTGGGTT | substitution
 | CAN1 | 8552 |  | + | G | C |  | CTCCCTTTATTATTGCTATT | [NAME] | substitution
067-064-A5A | ADE2 | 3894 | 131 |  | G | C |  | TTGAATGTTGGAAGAGATTT | GGTTTTCCATTCGTCTTGAA | substitution
 | CAN1 | 4025 | 2206 |  | G | A |  | [NAME] | [NAME] | substitution
 | URA3 | 6231 | 2079 |  | G | A |  | AGTAAAAAATTGTACTTGGC | GATAATGCCTTTAGCGGCTT | substitution
 | CAN1 | 8310 |  |  | G | A |  | CGAAGGGAGGTTCTTAGGTT | GGTTTCCTCTTTGATTAACG | substitution
067-064-A6A | ADE2 | 3513 | 4470 |  | G | C |  | ATATTAGGAGGGGGACAATT | GGACGTATGATTGTTGAGGC | substitution
 | CAN1 | 7983 |  |  | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
067-064-A8A | ADE2 | 5046 | 1269 |  | G | C |  | AGTACGAACGCTGCGCTGTT | GCTGTCAGACTGCTTGGCGC | substitution
 | URA3 | 6315 | 1824 |  | G | A |  | GTTTTTAGTAAACAAATTTT | GGACCTAATGCTTCAACTAA | substitution
 | CAN1 | 8139 |  |  | G | A |  | TTACGGTGAATTCGAGTTCT | GGTCGCTTCCATCAAAGTTT | substitution
067-064-A9A | ADE2 | 3869 | 4359 |  | C | - | -1 | GGAACAAGCCAGTGAGACGT | CCTATTGAATGTTGGAAGAG | deletion
 | CAN1 | 7983 | 245 | + | G | C |  | TGGAGAAACCCAGGTGCCTG | GGTCCAGGTATAATATCTAA | substitution
 | CAN1 | 8228 | 32 | + | G | C |  | TGGGGTTACCGGCCCAGTTG | [NAME] | substitution
 | CAN1 | 8260 |  | + | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
067-064-A12A | ADE2 | 4025 | 3958 |  | G | A |  | [NAME] | [NAME] | substitution
 | CAN1 | 7983 | 1215 | + | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
 | CAN1 | 9198 |  | + | G | A |  | TCATGAACCAAAGACTTTTT | GGACAAATTTTGGAATGTTG | substitution
067-064-A14A | ADE2 | 3505 | 4228 |  | G | A |  | CAGTTGGTATATTAGGAGGG | GACAATTGGGACGTATGATT | substitution
 | CAN1 | 7733 | 140 | + | G | C |  | AGCTTAAGCAAAGACATATT | GTATGATTGCCCTTGGTGGT | substitution
 | CAN1 | 7873 |  | + | G | C |  | TATTCTGTCACGCAGTCCTT | GGCGAAATGGCTACATTCAT | substitution
067-064-A19A | ADE2 | 4025 | 2490 |  | G | A |  | [NAME] | [NAME] | substitution
 | URA3 | 6515 | 1695 |  | G | A |  | GGTTTTTGTTCTGTGCAGTT | [NAME] | substitution
 | CAN1 | 8210 |  |  | G | - | -1 | TTTGTATGGTTTGTGGTGCT | GGGTTACCGGCCCAGTTGGA | deletion
067-064-A21A | ADE2 | 3937 | 3823 |  | G | C |  | CGAGGACTTTGGCATACGAT | GAAGAGGTAACTTCGTTGTA | substitution
 | CAN1 | 7760 | 319 | + | G | T |  | TTGCCCTTGGTGGTACTATT | GTACAGGTCTTTTCATTGGT | substitution
 | CAN1 | 8079 | 996 | + | G | C |  | GGCATGGATTAGTATTTTTT | GGTAATTATCACAATAATGA | substitution
 | CAN1 | 9075 |  | + | G | C |  | TTTCCTGTTCTTAGCTGTTT | GATCTTATTTCAATGCATAT | substitution
067-064-A22A | ADE2 | 3474 | 4605 |  | G | A |  | CGGACAAAACAATCAAGTAT | GATTCTAGAACAGTTGGTAT | substitution
 | CAN1 | 8079 |  |  | G | A |  | GGCATGGATTAGTATTTTTT | GGTAATTATCACAATAATGA | substitution
067-064-A23A | ADE2 | 5094 | 1221 |  | G | A |  | TCAAGTTATACAACGAAAAT | GAACAGTTTTTATTAAAGCA | substitution
 | URA3 | 6315 | 214 | + | G | A |  | GTTTTTAGTAAACAAATTTT | GGACCTAATGCTTCAACTAA | substitution
 | URA3 | 6529 | 1311 | + | G | C |  | GCAGTTGGGTTAAGAATACT | GGCAATTTCATGTTTCTTCA | substitution
 | CAN1 | 7840 | 470 | + | G | A |  | CTTATATCATATTTATTTAT | GGTTCTTTGGCATATTCTGT | substitution
 | CAN1 | 8310 |  | + | G | A |  | CGAAGGGAGGTTCTTAGGTT | GGTTTCCTCTTTGATTAACG | substitution
067-064-A24A | ADE2 | 4870 | 2896 |  | G | A |  | CAATTATCGCTGGAGCTGGT | GGGCTGCTCACTTGCCAGGT | substitution
 | CAN1 | 7766 | 217 | + | G | C |  | TTGGTGGTACTATTGGTACA | GTCTTTTCATTGGTTTATCC | substitution
 | CAN1 | 7983 | 188 | + | G | A |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
 | CAN1 | 8171 | 89 | + | G | A |  | TCAAAGTTTTAGCCATTATC | GGTTTCTAATATACTGTTTT | substitution
 | CAN1 | 8260 | 50 | + | G | C |  | TGGAGAAACCCAGGTGCCTG | GGTCCAGGTATAATATCTAA | substitution
 | CAN1 | 8310 | 888 | + | G | A |  | CGAAGGGAGGTTCTTAGGTT | GGTTTCCTCTTTGATTAACG | substitution
 | CAN1 | 9198 |  | + | G | C |  | TCATGAACCAAAGACTTTTT | GGACAAATTTTGGAATGTTG | substitution
067-064-A25A | ADE2 | 4924 | 3059 |  | G | A |  | TGACACCACTTCCTGTCATC | GTGTGCCCGTAAAAGGTTCT | substitution
 | CAN1 | 7983 |  |  | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution
067-064-A27A | 3′ LYS2 | 2656 | 2042 |  | G | C |  | ACGGCCAATTCATTTTCTTT | GGAGGATTCAAAGACGTAGC | substitution
 | ADE2 | 4698 | 3381 |  | G | A |  | CCATTGGTTGGAATCATCAT | GGATCAGACTCTGACTTGCC | substitution
 | CAN1 | 8079 |  |  | G | A |  | GGCATGGATTAGTATTTTTT | GGTAATTATCACAATAATGA | substitution
067-064-A28A | ADE2 | 3505 | 4478 |  | G | A |  | CAGTTGGTATATTAGGAGGG | GACAATTGGGACGTATGATT | substitution
 | CAN1 | 7983 |  |  | G | C |  | TTACATGTATTGGTTTTCTT | GGCAATCACTTTTGCCCTGG | substitution