Spreadsheet: 955904ca-944a-46ff-aef1-79d7be91b507
Sheet: Sheet1

Table S1. | Residual currents for all sequences used in this article. | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5
Name | Sequence | Ires (pA) | HWHH (pA) | No. Events | No. Pores
poly-dC | 5’- /biotin/ CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 37.7612999270073 | 0.8057426781021898 | 2065 | 8
poly-dA | 5’- /biotin/ AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAC CTG TCT CCC TGC CG | 85.92097153284672 | 2.043942226277372 | 1637 | 6
poly-dT | 5’- /biotin/ TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTC CTG TCT CCC TGC CG | 58.01058802919708 | 1.211269339051095 | 689 | 3
poly3-dG | 5’- /biotin/ AAA AAA AAA AAA AGG GAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 89.20570306569343 | 5.109363839416059 | 1287 | 4
poly4-mC | 5’- /biotin/ CCC CCC CCC CCC (mC)(mC)(mC) (mC)CC CCC CCC CCC CCC TGT CTC CCT GCC GCT GTC TCC CTG CCT GTC TCC CTG CCG | 38.85621043795621 | 0.283578544160584 | 878 | 3
poly-dC (5' lead) | 5’- CTG TCT CCC TGC CGC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CC /biotin/ | 83.18697343065693 | 1.425350569343066 | 1989 | 3
poly-dA (5' lead) | 5’- CTG TCT CCC TGC CGA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA /biotin/ | 91.39552408759124 | 1.809716609489051 | 568 | 3
poly-dT (5' lead) | 5’- CTG TCT CCC TGC CGT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TT /biotin/ | 49.80203737226277 | 0.9805677605839416 | 1279 | 3
poly3-dG (5' lead) | 5’- CTG TCT CCC TGC CGA AAA AAA AAA AAA AAA AAA AGG GAA AAA AAA AAA AA /biotin/ | 100.6989852554745 | 3.535184116788321 | 325 | 3
strand1 | 5’- /biotin/ CCC CCC CCC CCC CCC AAA AAT TTT TCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 42.68839722627737 | 2.400935503649635 | 495 | 2
strand2 | 5’- /biotin/ CCC CCC CCC CCC AAA TTT CCC CAA AAA CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 82.09206291970803 | 0.588891389781022 | 33 | 3
CinA11 | 5’- /biotin/ AAA AAA AAA ACA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 87.01588204379563 | 1.099090184306569 | 401 | 3
CinA12 | 5’- /biotin/ AAA AAA AAA AAC AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 85.37351627737226 | 2.827753912408759 | 748 | 3
CinA13 | 5’- /biotin/ AAA AAA AAA AAA CAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 56.36822226277372 | 5.687142215328467 | 330 | 4
 | second peak | 80.44969715328467 | 9.538998054744525 | 371 | 4
CinA14 | 5’- /biotin/ AAA AAA AAA AAA ACA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 51.44440313868613 | 0.4465366324817518 | 71 | 3
CinA15 | 5’- /biotin/ AAA AAA AAA AAA AAC AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 53.08676890510949 | 2.014766467153285 | 1922 | 5
CinA16 | 5’- /biotin/ AAA AAA AAA AAA AAA CAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 54.72585649635037 | 4.249006762773723 | 831 | 4
 | second peak | 76.07005510948906 | 3.641233083941606 | 589 | 4
CinA17 | 5’- /biotin/ AAA AAA AAA AAA AAA ACA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 86.46842678832117 | 2.163103894160584 | 75 | 3
CinA18 | 5’- /biotin/ AAA AAA AAA AAA AAA AAC AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA CTG TCT CCC TGC CG | 86.46842678832117 | 1.421924876277372 | 216 | 4
AinC11 | 5’- /biotin/ CCC CCC CCC CAC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 38.30875518248175 | 0.5726972043795621 | 156 | 3
AinC12 | 5’- /biotin/ CCC CCC CCC CCA CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 38.30875518248175 | 0.7050863091240877 | 68 | 3
AinC13 | 5’- /biotin/ CCC CCC CCC CCC ACC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 36.11893416058394 | 1.557215166058394 | 325 | 4
AinC14 | 5’- /biotin/ CCC CCC CCC CCC CAC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 35.02402364963504 | 0.751095497810219 | 584 | 4
AinC15 | 5’- /biotin/ CCC CCC CCC CCC CCA CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 40.49857620437956 | 1.190043154744526 | 498 | 6
AinC16 | 5’- /biotin/ CCC CCC CCC CCC CCC ACC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 41.59348671532847 | 0.7930561401459854 | 289 | 4
AinC17 | 5’- /biotin/ CCC CCC CCC CCC CCC CAC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 38.85621043795621 | 1.642693583941606 | 616 | 3
AinC18 | 5’- /biotin/ CCC CCC CCC CCC CCC CCA CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CCC CTG TCT CCC TGC CG | 39.40366569343066 | 0.8157575032846716 | 182 | 3
AinT13 | 5’- /biotin/ TTT TTT TTT TTT ATT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT CTG TCT CCC TGC CG | 70.59878072992701 | 5.552081397810219 | 671 | 3
AinT14 | 5’- /biotin/ TTT TTT TTT TTT TAT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT CTG TCT CCC TGC CG | 73.88351226277372 | 0.947261500729927 | 555 | 4
AinT15 | 5’- /biotin/ TTT TTT TTT TTT TTA TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT CTG TCT CCC TGC CG | 73.88351226277372 | 6.833356167883212 | 2231 | 3
AinT16 | 5’- /biotin/ TTT TTT TTT TTT TTT ATT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT CTG TCT CCC TGC CG | 61.84277481751825 | 3.285223259124087 | 860 | 3
SNP1-C13 | 5’- /biotin/ GCT GGA GAA AGG CAT GTG CAA ATT AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 51.99185839416058 | 4.461596423357665 | 353 | 4
 | second peak | 80.99715240875912 | 13.39560724817518 | 1321 | 4
SNP1-A13 | 5’- /biotin/ GCT GGA GAA AGG AAT GTG CAA ATT AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 96.86679846715327 | 2.498625124087591 | 454 | 3
SNP1-C14 | 5’- /biotin/ TGC TGG AGA AAG GCA TGT GCA AAT TAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 48.70712686131387 | 1.060604407664234 | 573 | 3
SNP1-A14 | 5’- /biotin/ TGC TGG AGA AAG GAA TGT GCA AAT TAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 89.20570306569343 | 5.194760302919708 | 599 | 3
SNP1-C15 | 5’- /biotin/ CTG CTG GAG AAA GGC ATG TGC AAA TTA AGA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 52.53931364963503 | 4.627635996350365 | 500 | 3
SNP1-A15 | 5’- /biotin/ CTG CTG GAG AAA GGA ATG TGC AAA TTA AGA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 82.09206291970803 | 1.989852335766423 | 312 | 3
SNP1-C16 | 5’- /biotin/ CCT GCT GGA GAA AGG CAT GTG CAA ATT AAG AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 80.99715240875912 | 13.80931295620438 | 1371 | 3
SNP1-A16 | 5’- /biotin/ CCT GCT GGA GAA AGG AAT GTG CAA ATT AAG AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 87.01588204379563 | 5.087727883211679 | 844 | 3
SNP2-C13 | 5’- /biotin/ AGG TAT GTA AAA CGT GCT ATG GAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 50.89694788321168 | 4.378658591240876 | 1027 | 3
SNP2-A13 | 5’- /biotin/ AGG TAT GTA AAA AGT GCT ATG GAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 82.63951817518247 | 2.329471284671533 | 1065 | 3
SNP2-C14 | 5’- /biotin/ GAG GTA TGT AAA ACG TGC TAT GGA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 49.25458211678832 | 0.7460143262773722 | 803 | 4
SNP2-A14 | 5’- /biotin/ GAG GTA TGT AAA AAG TGC TAT GGA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 90.30061357664233 | 3.251130237226277 | 1152 | 3
SNP2-C15 | 5’- /biotin/ GGA GGT ATG TAA AAC GTG CTA TGG AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 39.40366569343066 | 0.4695166405109489 | 125 | 5
 | second peak | 50.89694788321168 | 3.021166248175183 | 1124 | 5
SNP2-A15 | 5’- /biotin/ GGA GGT ATG TAA AAA GTG CTA TGG AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 39.40366569343066 | 2.280462565693431 | 1112 | 3
 | second peak | 93.03788985401461 | 2.091311857664234 | 874 | 3
SNP2-C16 | 5’- /biotin/ GGG AGG TAT GTA AAA CGT GCT ATG GAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 38.85621043795621 | 0.7518003054744525 | 80 | 3
 | second peak | 73.33605700729927 | 7.363764912408759 | 425 | 3
SNP2-A16 | 5’- /biotin/ GGG AGG TAT GTA AAA AGT GCT ATG GAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AAA AA | 39.95112094890511 | 2.365695120437956 | 185 | 3
 | second peak | 88.65824781021898 | 3.803174937956205 | 178 | 3
All DNA strands used in these experiments are listed above including the full sequence for each strand type, the residual current for this strand immobilized in M1-MspA with an applied voltage of 180 mV, the number of total events analyzed, and the number of M1-MspA pores that this strand interacted with.  The residual current and half-width-at-half-height (HWHH) listed is the peak value of a histogram of residual currents from all events and the corresponding half with half height (see Methods Section for complete analysis procedure).  Half with at half height values include broadening due to slight variations between experiments.