Spreadsheet: 25222de2-8c84-4dce-9755-8ea1d02c04a3
Sheet: Sheet1

Relative Quantification (RQ), ΔΔCT estimation | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12
Measurement of changes in mRNA levels (GFP transcript) by [NAME]. The expression levels of [NAME] was used as internal reference. Expression ratios obtained by [NAME]. RQ (relative quantification number) or DDCT was obtained from the equation DCT(x2)–DCT(WT), where DCT represents ¨[CT(ACT1)*E]-[CT(GFP)*E] and E is the PCR efficiency ([10(-1/m)]-1).  Expression levels were obtained from at least three experimental replicates.
Δx2 |  | WT
X1-GFP | DCт | X1-GFP | DCт |  | DDCт | DDCт SE | FoldChange |  | DDCт AVG | R | R_error | Significance
Fpr3 | -3.493298850695291 | Fpr3_wt | -3.503803280639648 |  | 0.01050442994435752 | 0.08894794665849726 | 0.99274532710841 |  | 0.01050442994435752 | -0.576831418853548 | 0.0655376294466487 | -1
Emi2 | -2.672392143249487 | Emi2_wt | -3.155937498168966 |  | 0.483545354919479 | 0.1371242719717801 | 1.398175397964 |  | 0.483545354919479 | -0.532727794767979 | 0.105987243703137 | -1
Fpr4 | -2.928395398712181 | Fpr4_wt | -2.261672966766358 |  | -0.6667224319458236 | 0.1083172578181766 | 0.6299361752098991 |  | -0.6667224319458236 | -0.386592643115128 | 0.0491307498741868 | -1
Ape2 | -3.310970680236807 | Ape2_wt | -3.292622270813188 |  | -0.01834840942361948 | 0.130432752518554 | 0.9873623856123895 |  | -0.01834840942361948 | -0.283221184379822 | 0.0714623926985786 | -1
Cot1 | -3.472758973696255 | Cot1_wt | -2.1110462283325 |  | -1.361712745363755 | 0.1366343718657469 | 0.3891200581590314 |  | -1.068417131825707 | -0.255716440098952 | 0.0595090855419202 | -1
Cot1.2 | -2.815749146461487 | Cot1_wt.2 | -2.040627628173827 |  | -0.7751215182876598 | 0.1334232223835957 | 0.5843394032655994
Nma2 | -3.338642240651469 | Nma2_wt | -4.06532611846924 |  | 0.7266838778177709 | 0.1056172554301112 | 1.654830987185694 |  | 0.7266838778177709 | -0.186465537153657 | 0.127352319026176 | 0
[NAME] | -1.615128783416766 | Lac1_wt | -1.789477727508545 |  | 0.1743489440917791 | 0.1478215523364088 | 1.128455043436433 |  | 0.1743489440917791 | -0.16581876886416 | 0.123788151775424 | 0
Hrb1 | -3.980450196329726 | Hrb1_wt | -3.09998804397583 |  | -0.8804621523538954 | 0.1357473446841018 | 0.5431933970333682 |  | -0.8804621523538954 | -0.0983348051106807 | 0.102204390126651 | 0
Pyc1 | -1.946348302205402 | Pyc1_wt | -2.516201040649415 |  | 0.5698527384440135 | 0.1185142969628625 | 1.484372047203976 |  | 0.5698527384440135 | -0.0914475644266222 | 0.033359031862801 | 0
Kes1 | -1.786583915074667 | Kes1_wt | -2.417071254730224 |  | 0.630487339655557 | 0.0900240332014482 | 1.54808784637394 |  | 0.630487339655557 | -0.0130670061727707 | 0.0333587427949127 | 0
Sbp1 | -3.185811297734413 | Sbp1_wt | -2.43466197128296 |  | -0.7511493264514533 | 0.09584976624951361 | 0.5941300537905244 |  | -0.7511493264514533 | -0.00757955773662586 | 0.0421817988968661 | 0
Emp70 | -3.388520720799765 | Emp70_wt | -3.734510643157956 |  | 0.3459899223581915 | 0.3596773709056558 | 1.27102281081299 |  | 0.3459899223581915 | 0.0433475316650461 | 0.0418812445312829 | 0
Pir1 | -1.844515515136723 | Pir1_wt | -2.058515270233154 |  | 0.2139997550964319 | 0.1196695467006789 | 1.159899457607593 |  | 0.2139997550964319 | 0.0509451717076739 | 0.0736271402213306 | 0
Nmd5 | -2.350591643015543 | Nmd5_wt | -3.082292239379855 |  | 0.7317005963643126 | 0.1576467921539849 | 1.660595387753275 |  | 0.7317005963643126 | 0.0520118595447947 | 0.0814218091209269 | 0
Caf120 | -3.604058017730713 | Caf120_wt | -3.177601046142295 |  | -0.4264569715884172 | 0.1176371543326147 | 0.7440869017916473 |  | -0.4264569715884172 | 0.261230480589589 | 0.16342504189431 | 0
Bmh1 | -2.497630675760914 | Bmh1_wt | -1.800168702850338 |  | -0.6974619729105758 | 0.1377865818638829 | 0.6166560906200915 |  | -0.6974619729105758 | 0.29479365888008 | 0.0608584885759887 | 1
Sam1 | -3.138031296539306 | Sam1_wt | -3.541305028915405 |  | 0.4032737323760993 | 0.09759215309330938 | 1.322505509334957 |  | 0.7246351215490376 | 0.313388029227764 | 0.04275428153629 | 1
Sam1.2 | -2.700369610341233 | Sam1_wt.2 | -3.746366121063209 |  | 1.045996510721976 | 0.1591727223018057 | 2.064792077088965
Hsc82 | -2.692024568684873 | Hsc82_wt | -2.209048575592309 |  | -0.4829759930925643 | 0.1446609652953204 | 0.7155001660642644 |  | -0.4829759930925643 | 0.341593600821067 | 0.0420739362605013 | 1
Mmf1 | -3.105229499562575 | Mmf1_wt | -4.810251509246775 |  | 1.7050220096842 | 0.1110364364555119 | 3.260339068802695 |  | 1.7050220096842 | 0.347638687473653 | 0.107302213066916 | 1
Cue4 | -2.290316575368247 | Cue4_wt | -2.938953115081787 |  | 0.6486365397135407 | 0.09604992765791145 | 1.567685908977261 |  | 0.6486365397135407 | 0.432988269123509 | 0.0827771227286233 | 1
[NAME] | -3.295379130045572 | Imd3_wt | -6.055014851684599 |  | 2.759635721639027 | 0.1252880694415579 | 6.772252299323035 |  | 2.759635721639027 | 0.516351137915862 | 0.0323787784974432 | 1
Pgm2 | -3.31079930973053 | Pgm2_wt | -4.594236532287576 |  | 1.283437222557046 | 0.08529212061984066 | 2.434182307769949 |  | 1.283437222557046 | 0.552495732960129 | 0.130834906930254 | 1
Dot6 | -3.62917386856079 | Dot6_wt | -3.856954195150769 |  | 0.2277803265899787 | 0.0697159331886821 | 1.171031859300262 |  | 0.2277803265899787 | 0.69741506835003 | 0.152059445903035 | 1
Rga2 | -3.341189407348635 | Rga2_wt | -4.264712269337945 |  | 0.9235228619893103 | 0.126032182394591 | 1.896741223254459 |  | 0.9235228619893103 | 0.720848036672333 | 0.0943332411433284 | 1
Imd4 | -3.155453804016113 | Imd4_wt | -2.618116446838386 |  | -0.5373373571777278 | 0.1284675121429788 | 0.6890414334554634 |  | -0.5373373571777278 | 0.750882240395231 | 0.0547148180543002 | 1
Gin4 | -4.315295171737671 | Gin4_wt | -3.26144677734375 |  | -1.053848394393921 | 0.1444681405502853 | 0.4816815618567591 |  | -1.053848394393921 | 0.824969149615322 | 0.127653309298424 | 1
Lys21 | -3.662624610900879 | Lys21_wt | -5.217260638061513 |  | 1.554636027160635 | 0.1130434580247523 | 2.937596056507522 |  | 1.554636027160635 | 0.898394958372542 | 0.104229679045562 | 1
Htb1 | -4.273390361658718 | Htb1_wt | -5.179998583221437 |  | 0.9066082215627187 | 0.1091386504647624 | 1.874633048211611 |  | 0.9066082215627187 | 0.905292024007721 | 0.0782640276525207 | 1
Hor2 | -2.273704510370889 | Hor2_wt | -2.247232543945275 |  | -0.02647196642561367 | 0.1209495985659962 | 0.9818183485077571 |  | -0.02647196642561367 | 1.18152936698224 | 0.185226670995905 | 1
Sam2 | -2.269265819549561 | Sam2_wt | -2.888735225658095 |  | 0.6194694061085344 | 0.08864226941966374 | 1.536310053784113 |  | 0.7273105984517108 | 1.18153991935371 | 0.0768546557435985 | 1
Sam2.2 | -1.666713810984319 | Sam2_wt.2 | -2.063613352203369 |  | 0.3968995412190501 | 0.08620121830474134 | 1.31667523518807
Sam2.3 | -1.273442532221475 | Sam2_wt.3 | -2.439005380249022 |  | 1.165562848027547 | 0.07049462987561676 | 2.243207142399607
Hsp82 | -1.286060279697934 | Hsp82_wt | -2.365115643310547 |  | 1.079055363612613 | 0.08667647313281533 | 2.112652322459855 |  | 1.079055363612613 | 1.40626335514823 | 0.0676578779588893 | 1
Hxk1 | 0.6867513290405274 | Hxk1_wt | 0.2798518908818573 |  | 0.4068994381586701 | 0.09575196266300239 | 1.325833340982613 |  | 0.4687624724896809 | 3.47433279159847 | 0.333877445518088 | 1
Hxk1.2 | 0.6575049261474739 | Hxk1_wt.2 | 0.1268794193267822 |  | 0.5306255068206917 | 0.1304385498269985 | 1.444555373169077