Spreadsheet: 99c41a27-4b2d-4e0e-a71a-ca4033bc3259
Sheet: Sheet1

Target gene (accession ID)- hyperlinked to NCBI | Primers for dsRNA synthesis | Unnamed: 2 | qRT-PCR primers | Unnamed: 4
 | Name | Sequence 5’-3’ | Name | Sequence 5’-3’
Cytochrome Oxydase (AF216698) |  |  | COX-q-s | TACGGTTGGTGGTGTCACAG
 |  |  | COX-q-as | ACGGCCATCACCATACTAGC
mCherry control (AY678264) | [NAME] | TAATACGACTCACTATAGGGATGGTGAGCAAGGGCGAGGAG
 | Cherry-as | TTACTTGTACAGCTCGTCC
 | [NAME] | ATGGTGAGCAAGGGCGAGGAG
 | [NAME] | TAATACGACTCACTATAGGGTTACTTGTACAGCTCGTCC
[NAME] (AJ506157) | CB1-T7-s | AAGTAATACGACTCACTATAGGGATGCTCACATCTATTTTGTGTATTGC | BqrtSmCB1.1f | ACTTGGTGGGCACGCTATAC
Smp_103610 | CB1-as | CAATACCTTCCTTCACCCAGTAATC | BqrtSmCB1.1r | GGCTGTTACCTCGGATTCAA
 | CB1-s | ATGCTCACATCTATTTTGTGTATTGC
 | CB1-T7-as | AAGTAATACGACTCACTATAGGGCAATACCTTCCTTCACCCAGTAATC
Cathepsin C (Z32531) | CC_FWD1_T7 | AAGTAATACGACTCACTATAGGGACACCGATTCGTAACCAAGG | CC-qA-s | ACACCGATTCGTAACCAAGG
Smp_019030 | CC_R2 | GCTCGATTTCTGAGTTCATCTATTG | CC-qA-as | ATCCTTCGGAGTAGGGGCTA
 | CC_FWD1 | ACACCGATTCGTAACCAAGG
 | CC_R2_T7 | AAGTAATACGACTCACTATAGGGGCTCGATTTCTGAGTTCATCTATTG
Cathepsin D (U60995) | CD-R1_T7 | AAGTAATACGACTCACTATAGGGATCCTTCGGAGTAGGGGCTA | CD-qD-s | GGGTGCCGTCAAAATACTGT
Smp_013040.1 | CD_FWD3 | ATTGGTACGCCACCTCAGAC | CD-qD-as | ACCAACCCTGGTTGTTGTGT
 | CD_R1 | ATCCTTCGGAGTAGGGGCTA | CD-qB-s | ATTGGTACGCCACCTCAGAC
 | CD_FWD3_T7 | [NAME] | CD-qB-as | AGCCCAACTGAAGGGAATCT
[NAME] (AY334553.1) | Cyst-T7-s | TAATACGACTCACTATAGGGAACCGTCTGCAGAGGAAAAA | Cyst-qB-s | GATGCGTTGGAATATTTCTG
Smp_006390 | Cyst-rev | ATTCCAACGCATCACTTTCC | Cyst-qB-as | TAACACTCAACCCACAATCA
 | Cyst-fwd | AACCGTCTGCAGAGGAAAAA
 | Cyst-T7-as | TAATACGACTCACTATAGGGATTCCAACGCATCACTTTCC
Sm29 (XP_002578407.1) | [NAME] | TAATACGACTCACTATAGGGTGGCTGGGAGTATATTGGAA | Sm29-qC-s | ACAGATCTATGTAACGGAATGAC
Smp_072190 | [NAME] | TCCTATGCAAACAAACAGAACAA | Sm29-qC-as | GTGAATGTGTATGGAAGAAGG
 | [NAME] | TGGCTGGGAGTATATTGGAA
 | [NAME] | TAATACGACTCACTATAGGGTCCTATGCA
[NAME] (AJ312106) | CB2 fwd T7 | TAATACGACTCACTATAGGGCATCGTGTGGTTCTTGTTGG | qRT-CB2-A-f | GAGGCCAATACGACTTGGAA
Smp_141610 | [NAME] | ATTTGGGAAATCGGCATACA | qRT-CB2-A-rev | CTACCCTCGCATCAAAGCTC
 | CB2 fwd | CATCGTGTGGTTCTTGTTGG | qRT-CB2-B-f | CATCGTGTGGTTCTTGTTGG
 | [NAME] | TAATACGACTCACTATAGGGATTTGGGAAATCGGCATACA | qRT-CB2-B-rev | AATGTGGGAAGCCACCATTA
[NAME] ([NAME]) | [NAME] | TAATACGACTCACTATAGGGTAGCGCTGAAAATGATGCAG | Q-AnnexF A | GGACTTGGAACACGAGAGGA
Smp_077720 | AnnexA-R | TCCTCTCGTGTTCCAAGTCC | Q-AnnexR A | CTTGCGAAAATCTCCTGATG
 | [NAME] | TAGCGCTGAAAATGATGCAG
 | [NAME] | TAATACGACTCACTATAGGGTCCTCTCGTGTTCCAAGTCC
Glycogen synthase kinase-3 (XP_002572305.1) | [NAME] | GGATCCTAATACGACTCACTATAGGTCAGCCAATTTTTCCTGGAG | [NAME] | AAAGATTCCTGCCATGAAGA
Smp_008260 | GSK3-as | GCGGTTGTGGTTGTTCTAC | [NAME] | GACGAGAAACCTGAGTCGAA
 | [NAME] | TCAGCCAATTTTTCCTGGAG
 | GSK3-as-T7 | GGATCCTAATACGACTCACTATAGGGCGGTTGTGGTTGTTCTAC
[NAME] aminopeptidase (XP_002572573.1) | MAP-s-T7 | GGATCCTAATACGACTCACTATAGGGAACGCGGATTGAATGCAG | MAP quant-s/2 | [NAME]
Smp_011120 | MAP-as | CGTGTGCTCCCATTGAGCA | MAP quant-as/2 | ACGGCTAGTGCGTTCAAGTT
 | MAP-s | GAACGCGGATTGAATGCAG
 | MAP-as-T7 | GGATCCTAATACGACTCACTATAGGCGTGTGCTCCCATTGAGCA
N-myristoyltransferase (XP_002571399.1 ) | NMT-s-T7 | GGATCCTAATACGACTCACTATAGGCAGCTATTCCATGCCATC | NMT-q-D-s | CAGTCATGAAAAGCAAACAA
Smp_121420 | NMT-as | GTGATTTGGCCATCTCCACT | NMT-q-D-as | CCAAATTTCAACTCTTCCAG
 | NMT-s | GCAGCTATTCCATGCCATC
 | NMT-as-T7 | GGATCCTAATACGACTCACTATAGGTGATTTGGCCATCTCCACT
Neuroendocrine convertase (XP_002569775.1) | NEC-s-T7 | GGATCCTAATACGACTCACTATAGGTGGTCGTGGAGTGATTACAG | NEC-q-E-s | CAACAACCACCATATGCTCA
Smp_077980 | NEC-as | CTGCCGCTGATGTACCTG | NEC-q-E-as | [NAME]
 | NEC-s | TGGTCGTGGAGTGATTACAG
 | NEC-as-T7 | GGATCCTAATACGACTCACTATAGGCTGCCGCTGATGTACCTG
Protein phosphatase 2A (XP_002574514.1) | [NAME] | GGATCCTAATACGACTCACTATAGGTGCAGTGCGTAGATTCTC | PP2Aq(c)-s | AGGTGCTGGCTATTTATTTG
Smp_030710 | [NAME] | CAGTCTCTTCCGGATCAGA | PP2Aq(c)-as | ATTTGGAGCAGACCATACAG
 | PP2A-s | GTGCAGTGCGTAGATTCTC
 | [NAME] | GGATCCTAATACGACTCACTATAGGCAGTCTCTTCCGGATCAGA
 |  |  | This set of CB2 qRT primers (Set B) was designed to amplify within the sequence of cDNA used to generate dsRNA. See [NAME] and text for details.