Spreadsheet: f76cb808-85d4-43bb-bc88-8cee7eec2210
Sheet: Sheet1

Supplementary Table 4.  Moehle et al. "The Yeast SR-like Protein Npl3 Links Chromatin Modification to mRNA processing" | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4
Genes that when deleted restored growth to npl3∆ at 16°C after 4 or 8 days
Systematic Name | [NAME] | length of time | notes | Putative Function
YER170W | ADK2 | suppressed after 4 days |  | Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
YFR011C | AIM13 | suppressed after 4 days |  | Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YML087C | AIM33 | suppressed after 4 days |  | Putative protein of unknown function, highly conserved across species and orthologous to human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss
YOL053W | AIM39 | suppressed after 4 days |  | Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
YML086C | ALO1 | suppressed after 4 days |  | D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress
YDR242W | AMD2 | suppressed after 4 days |  | Putative amidase
YJR047C | ANB1 | suppressed after 4 days |  | Translation elongation factor eIF-5A, previously thought to function in translation initiation; similar to and functionally redundant with Hyp2p; undergoes an essential hypusination modification; expressed under anaerobic conditions
YDR530C | [NAME] | suppressed after 4 days |  | Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase II (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa1p
YNL094W | APP1 | suppressed after 4 days |  | Protein of unknown function, interacts with Rvs161p and Rvs167p; computational analysis of protein-protein interactions in large-scale studies suggests a possible role in actin filament organization
YIL040W | [NAME] | suppressed after 4 days |  | Protein required for nuclear envelope morphology, nuclear pore complex localization, mRNA export from the nucleus; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YJL075C | APQ13 | suppressed after 4 days |  | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate
YNL008C | ASI3 | suppressed after 4 days |  | Putative integral membrane E3 ubiquitin ligase; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
YMR098C | ATP25 | suppressed after 4 days |  | Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
YER114C | [NAME] | suppressed after 4 days |  | Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain
YER164W | CHD1 | suppressed after 4 days | confirmed using tetrad analysis | Chromatin remodeler that regulates various aspects of transcription; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
YOR061W | CKA2 | suppressed after 4 days |  | [NAME]' catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and [NAME], has many substrates including transcription factors and all RNA polymerase
[NAME] | [NAME] | suppressed after 4 days |  | Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
YJR032W | CPR7 | suppressed after 4 days |  | Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity
YKL139W | CTK1 | suppressed after 4 days | confirmed using tetrad analysis | Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YMR238W | DFG5 | suppressed after 4 days |  | Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to [NAME]
YGL043W | [NAME] | suppressed after 4 days |  | General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites
YML080W | DUS1 | suppressed after 4 days |  | [NAME] synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17
YPR023C | EAF3 | suppressed after 4 days | confirmed using tetrad analysis | Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
[NAME] | ECM21 | suppressed after 4 days |  | Protein involved in regulating the endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements
YNL084C | END3 | suppressed after 4 days |  | EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YDR518W | EUG1 | suppressed after 4 days |  | Protein disulfide isomerase of the endoplasmic reticulum lumen, function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
YIL098C | [NAME] | suppressed after 4 days |  | Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p
YKR049C | FMP46 | suppressed after 4 days |  | Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YNR047W | FPK1 | suppressed after 4 days |  | Ser/Thr protein kinase that regulates the putative phospholipid translocases Lem3p-Dnf1p/Dnf2p; phosphorylates and inhibits upstream inhibitory kinase, Ypk1p; localizes to the cytoplasm, early endosome/TGN compartments, and plasma membrane
YOR381W | [NAME] | suppressed after 4 days |  | Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
[NAME] | GAL2 | suppressed after 4 days |  | Galactose permease, required for utilization of galactose; also able to transport glucose
YJR040W | GEF1 | suppressed after 4 days |  | Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels
YNL153C | GIM3 | suppressed after 4 days |  | Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
YOL151W | GRE2 | suppressed after 4 days |  | 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway
YDR174W | [NAME] | suppressed after 4 days |  | Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
YNL108C | HUF | suppressed after 4 days |  | Putative protein of unknown function with similarity to Tfc7p and prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus
YIL154C | IMP2' | suppressed after 4 days |  | Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YOR013W | IRC11 | suppressed after 4 days |  | Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci
YIL094C | LYS12 | suppressed after 4 days |  | Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate
YJL013C | MAD3 | suppressed after 4 days |  | Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover
YCR020C-A | MAK31 | suppressed after 4 days |  | Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family
[NAME] | MET18 | suppressed after 4 days |  | DNA repair and TFIIH regulator, required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription; involved in telomere maintenance
YDR296W | MHR1 | suppressed after 4 days |  | Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
YDR205W | MSC2 | suppressed after 4 days |  | Member of the cation diffusion facilitator family, localizes to the endoplasmic reticulum and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
YDR277C | MTH1 | suppressed after 4 days |  | Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and [NAME] glucose sensors; phosphorylated by [NAME], triggering Mth1p degradation
YDL040C | NAT1 | suppressed after 4 days |  | Subunit of the N-terminal acetyltransferase [NAME] (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
YHR073W | OSH3 | suppressed after 4 days |  | Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YER178W | PDA1 | suppressed after 4 days |  | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose
[NAME] | [NAME] | suppressed after 4 days |  | S-adenosylmethionine transporter of the mitochondrial inner membrane, member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth
YBR106W | PHO88 | suppressed after 4 days |  | Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YER053C | PIC2 | suppressed after 4 days |  | Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
YML061C | PIF1 | suppressed after 4 days |  | DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis
YPL148C | PPT2 | suppressed after 4 days |  | [NAME]:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YIL117C | PRM5 | suppressed after 4 days |  | Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling
YDR529C | QCR7 | suppressed after 4 days |  | Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
[NAME] | QDR1 | suppressed after 4 days |  | Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, ketoconazole, fluconazole, and barban
YDR217C | RAD9 | suppressed after 4 days |  | DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate
YMR075W | RCO1 | suppressed after 4 days | confirmed using random sporulation | Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p
YPL208W | RKM1 | suppressed after 4 days |  | SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit protein L23a (RPL23A and RPL23B)
YFL052W | ROP1 | suppressed after 4 days |  | Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to [NAME], low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity
YEL054C | RPL12A | suppressed after 4 days |  | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins
YNL069C | RPL16B | suppressed after 4 days |  | N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Ap, E. coli [NAME] and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p
[NAME] | RPL2B | suppressed after 4 days |  | Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures
YML063W | RPS1B | suppressed after 4 days |  | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein
YMR266W | RSN1 | suppressed after 4 days |  | Membrane protein of unknown function; overexpression suppresses [NAME] sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane
YJR004C | SAG1 | suppressed after 4 days |  | Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor
YER129W | SAK1 | suppressed after 4 days |  | Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
YPL047W | SGF11 | suppressed after 4 days | confirmed using tetrad analysis | Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
[NAME] | SGF29 | suppressed after 4 days | confirmed using tetrad analysis | Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA recruitment to target promoters and H3 acetylation
YBR077C | SLM4 | suppressed after 4 days |  | Component of the EGO complex, which is involved in the regulation of microautophagy, and of the [NAME] complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
YDR104C | [NAME] | suppressed after 4 days |  | Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
YLR375W | [NAME] | suppressed after 4 days |  | Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids
[NAME] | SUM1 | suppressed after 4 days |  | Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
YGL162W | SUT1 | suppressed after 4 days |  | Transcription factor of the Zn[II]2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression
YDR346C | SVF1 | suppressed after 4 days |  | Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
YML081W | TDA9 | suppressed after 4 days |  | DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene
YER113C | TMN3 | suppressed after 4 days |  | Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation
YER175C | TMT1 | suppressed after 4 days |  | Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
YDR074W | TPS2 | suppressed after 4 days |  | Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the [NAME]-cAMP pathway
YMR223W | UBP8 | suppressed after 4 days | confirmed using tetrad analysis | Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B
YDR200C | [NAME] | suppressed after 4 days |  | Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
YER072W | [NAME] | suppressed after 4 days |  | Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity
YJL012C | VTC4 | suppressed after 4 days |  | Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion
YER024W | YAT2 | suppressed after 4 days |  | Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
YER123W | YCK3 | suppressed after 4 days |  | Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
YML082W |  | suppressed after 4 days |  | Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene
YML083C |  | suppressed after 4 days |  | Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
YML084W |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
[NAME] |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage
YML079W |  | suppressed after 4 days |  | Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm
YBR144C |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene
YOR268C |  | suppressed after 4 days |  | Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene
YMR075C-A |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W
YHL037C |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJR037W |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces
YIL029C |  | suppressed after 4 days |  | Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YNR042W |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene [NAME]
YGL088W |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing
[NAME] |  | suppressed after 4 days |  | Putative protein of unknown function
YJL028W |  | suppressed after 4 days |  | Protein of unknown function; may interact with ribosomes, based on co-purification experiments
[NAME] |  | suppressed after 4 days |  | Protein of unknown function; localizes to the mitotic spindle; overexpression of [NAME] affects endocytic protein trafficking
YGL242C |  | suppressed after 4 days |  | Putative protein of unknown function; deletion mutant is viable
YMR304C-A |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10
YNR005C |  | suppressed after 4 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR008C-A |  | suppressed after 4 days |  | Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
YKL106W | [NAME] | suppressed after 8 days |  | Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
YOR239W | ABP140 | suppressed after 8 days |  | AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift
[NAME] | ACF4 | suppressed after 8 days |  | Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
YPL267W | ACM1 | suppressed after 8 days |  | Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and [NAME]; cell cycle regulated protein
YDR511W | ACN9 | suppressed after 8 days |  | Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes
YGL256W | ADH4 | suppressed after 8 days |  | Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
YBR145W | ADH5 | suppressed after 8 days |  | Alcohol dehydrogenase isoenzyme V; involved in ethanol production
YCR105W | ADH7 | suppressed after 8 days |  | NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance
YMR282C | AEP2 | suppressed after 8 days |  | Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YOR129C | AFI1 | suppressed after 8 days |  | Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, [NAME]; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
YPR021C | AGC1 | suppressed after 8 days |  | Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis
YDR524C | AGE1 | suppressed after 8 days |  | ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
YDR214W | AHA1 | suppressed after 8 days |  | Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock
YNR074C | AIF1 | suppressed after 8 days |  | Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase
YAL046C | AIM1 | suppressed after 8 days |  | Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss
YHR198C | AIM18 | suppressed after 8 days |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YIL087C | AIM19 | suppressed after 8 days |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth
YIR003W | AIM21 | suppressed after 8 days |  | Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton
YBR108W | AIM3 | suppressed after 8 days |  | Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss
YPR004C | AIM45 | suppressed after 8 days |  | Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, [NAME]; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response
YOR175C | ALE1 | suppressed after 8 days |  | Broad-specificity lysophospholipid acyltransferase, part of [NAME] family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids
YNR030W | ALG12 | suppressed after 8 days |  | Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
YML035C | AMD1 | suppressed after 8 days |  | AMP deaminase, tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; may be involved in regulation of intracellular adenine nucleotide pools
YLR102C | APC9 | suppressed after 8 days |  | Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
[NAME] | APE4 | suppressed after 8 days |  | Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family
YPR029C | APL4 | suppressed after 8 days |  | Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
YLR170C | APS1 | suppressed after 8 days |  | Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
YLR118C | APT1 | suppressed after 8 days |  | Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
YDR441C | APT2 | suppressed after 8 days |  | Apparent pseudogene, not transcribed or translated under normal conditions; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity
YLR392C | ART10 | suppressed after 8 days |  | Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
YGR097W | ASK10 | suppressed after 8 days |  | Component of RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; paralogous to Rgc1p
YDR184C | [NAME] | suppressed after 8 days |  | Nuclear protein, possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern
YGR177C | ATF2 | suppressed after 8 days |  | Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
YBR128C | ATG14 | suppressed after 8 days |  | Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); [NAME] targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; homolog of human [NAME]
YCR068W | [NAME] | suppressed after 8 days |  | Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway
YDL113C | ATG20 | suppressed after 8 days |  | Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
[NAME] | ATG32 | suppressed after 8 days |  | Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane
YPR026W | ATH1 | suppressed after 8 days |  | Acid trehalase required for utilization of extracellular trehalose
YNR002C | [NAME] | suppressed after 8 days |  | Putative transmembrane protein involved in export of ammonia, a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of [NAME] and Y. lipolytica Gpr1p
YLR393W | ATP10 | suppressed after 8 days |  | Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6
YNR020C | ATP23 | suppressed after 8 days |  | Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex
YAL020C | ATS1 | suppressed after 8 days |  | Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
YOR011W | AUS1 | suppressed after 8 days |  | Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen
YIL088C | AVT7 | suppressed after 8 days |  | Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
[NAME] | BAG7 | suppressed after 8 days |  | Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p
YPL161C | BEM4 | suppressed after 8 days |  | Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YGR282C | BGL2 | suppressed after 8 days |  | Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance
YBR270C | BIT2 | suppressed after 8 days |  | Subunit of TORC2, a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; similar to Bit61p
YDR099W | BMH2 | suppressed after 8 days |  | 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, [NAME]/MAPK signaling, and rapamycin-sensitive signaling
YJR025C | [NAME] | suppressed after 8 days |  | 3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YJR078W | BNA2 | suppressed after 8 days |  | Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and [NAME]
YGR041W | [NAME] | suppressed after 8 days |  | Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole
YDL099W | BUG1 | suppressed after 8 days |  | Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, [NAME]; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
[NAME] | BUL1 | suppressed after 8 days |  | Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases
[NAME] | CAF130 | suppressed after 8 days |  | Part of the evolutionarily-conserved [NAME]-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation
YKR036C | [NAME] | suppressed after 8 days |  | WD40 repeat-containing protein associated with the [NAME]-NOT complex, interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission
YLR220W | CCC1 | suppressed after 8 days |  | Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation
YAL021C | [NAME] | suppressed after 8 days |  | Component of the [NAME]-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YDR254W | CHL4 | suppressed after 8 days |  | Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore subcomplex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p
YJL099W | CHS6 | suppressed after 8 days |  | Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, [NAME], Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane
YER030W | CHZ1 | suppressed after 8 days |  | Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif
YGR207C | CIR1 | suppressed after 8 days |  | Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
YJL158C | CIS3 | suppressed after 8 days |  | Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
YCR005C | CIT2 | suppressed after 8 days |  | Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors
[NAME] | [NAME] | suppressed after 8 days | confirmed using tetrad analysis | Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and [NAME], has many substrates including transcription factors and all RNA polymerase
YFR014C | CMK1 | suppressed after 8 days |  | Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk2p and mammalian [NAME]
YML057W | CMP2 | suppressed after 8 days |  | Calcineurin A; one isoform (the other is [NAME]) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
YOL008W | COQ10 | suppressed after 8 days |  | Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
YLL018C-A | COX19 | suppressed after 8 days |  | Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
YHR116W | COX23 | suppressed after 8 days |  | Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
YDR155C | CPR1 | suppressed after 8 days |  | Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A
YCR069W | CPR4 | suppressed after 8 days |  | Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
YMR025W | CSI1 | suppressed after 8 days |  | Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
YDR151C | CTH1 | suppressed after 8 days |  | Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis
YDR371W | CTS2 | suppressed after 8 days |  | Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect
[NAME] | CTT1 | suppressed after 8 days |  | Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide
[NAME] | CUE1 | suppressed after 8 days |  | Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination
YCR017C | CWH43 | suppressed after 8 days |  | Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
YKL096W | CWP1 | suppressed after 8 days |  | Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance
YJR048W | CYC1 | suppressed after 8 days |  | Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration
YJR152W | DAL5 | suppressed after 8 days |  | Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
[NAME] | DAL80 | suppressed after 8 days |  | Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Deh1p
YDR020C | DAS2 | suppressed after 8 days |  | Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
[NAME] | [NAME] | suppressed after 8 days |  | Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis
YOR163W | [NAME] | suppressed after 8 days |  | Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; has high specificity for diadenosine hexa- and pentaphosphates; exhibits endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; member of the MutT family of nucleotide hydrolases
YDL024C | DIA3 | suppressed after 8 days |  | Protein of unknown function, involved in invasive and pseudohyphal growth
YLR348C | DIC1 | suppressed after 8 days |  | Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
YDR263C | DIN7 | suppressed after 8 days |  | Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination
YMR162C | DNF3 | suppressed after 8 days |  | Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase
YIL103W | [NAME] | suppressed after 8 days |  | Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
YGL196W | [NAME] | suppressed after 8 days |  | D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate
YBR007C | [NAME] | suppressed after 8 days |  | Deletion suppressor of mpt5 mutation
YMR276W | DSK2 | suppressed after 8 days |  | Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome
YPR017C | DSS4 | suppressed after 8 days |  | Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
YKR054C | DYN1 | suppressed after 8 days |  | Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p
YLR284C | ECI1 | suppressed after 8 days |  | Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced
YDR125C | ECM18 | suppressed after 8 days |  | Protein of unknown function, similar to Rlp24p
YLR390W | ECM19 | suppressed after 8 days |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOR092W | ECM3 | suppressed after 8 days |  | Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation
[NAME] | ECM34 | suppressed after 8 days |  | Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
YGR007W | ECT1 | suppressed after 8 days |  | Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: [NAME]transferases
YPL037C | EGD1 | suppressed after 8 days |  | [NAME] of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b
YBR177C | EHT1 | suppressed after 8 days |  | Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane
YPL046C | ELC1 | suppressed after 8 days |  | [NAME], conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated [NAME] polymerase II to trigger its proteolysis; plays a role in global genomic repair
YCL045C | EMC1 | suppressed after 8 days |  | Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p
[NAME] | EMC5 | suppressed after 8 days |  | Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance
[NAME] | EMI1 | suppressed after 8 days |  | Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor [NAME], also required for sporulation; contains twin cysteine-x9-cysteine motifs
[NAME] | EMI2 | suppressed after 8 days |  | Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor [NAME]; required for sporulation; expression is regulated by glucose-repression transcription factors Mig1/2p
YLR206W | ENT2 | suppressed after 8 days |  | Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus
YDR153C | ENT5 | suppressed after 8 days |  | Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
[NAME] | [NAME] | suppressed after 8 days |  | Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YNL280C | ERG24 | suppressed after 8 days |  | C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions
[NAME] | ERG5 | suppressed after 8 days |  | C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YAR002C-A | ERP1 | suppressed after 8 days |  | Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles
YLR300W | EXG1 | suppressed after 8 days |  | Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes
YDR261C | EXG2 | suppressed after 8 days |  | Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YJL157C | FAR1 | suppressed after 8 days |  | Cyclin-dependent kinase inhibitor that mediates cell cycle arrest in response to pheromone; also forms a complex with [NAME], Ste4p, and [NAME] that may specify the direction of polarized growth during mating; potential Cdc28p substrate
YER056C | FCY2 | suppressed after 8 days |  | Purine-cytosine permease, mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation
YDR539W | FDC1 | suppressed after 8 days |  | Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm
YCR089W | FIG2 | suppressed after 8 days |  | Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating
[NAME] | FIT3 | suppressed after 8 days |  | Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
YLL029W | FRA1 | suppressed after 8 days |  | Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation
YGL220W | FRA2 | suppressed after 8 days |  | Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YOR384W | FRE5 | suppressed after 8 days |  | Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOL152W | FRE7 | suppressed after 8 days |  | Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels
YCL026C-A | FRM2 | suppressed after 8 days |  | Protein of unknown function, involved in the integration of lipid signaling pathways with cellular homeostasis; expression induced in cells treated with the mycotoxin patulin; has similarity to bacterial nitroreductases
YCL027W | [NAME] | suppressed after 8 days |  | Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate
YGL254W | FZF1 | suppressed after 8 days |  | Transcription factor involved in sulfite metabolism, sole identified regulatory target is SSU1, overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers
YBR019C | GAL10 | suppressed after 8 days |  | UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers
YOL051W | GAL11 | suppressed after 8 days |  | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
[NAME] | GAL7 | suppressed after 8 days |  | Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism
YMR215W | GAS3 | suppressed after 8 days |  | Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation
[NAME] | GAS4 | suppressed after 8 days |  | 1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall
YDR019C | GCV1 | suppressed after 8 days |  | T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
[NAME] | GCV2 | suppressed after 8 days |  | P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm
YOR120W | GCY1 | suppressed after 8 days |  | Putative NADP(+) coupled glycerol dehydrogenase, proposed to be involved in an alternative pathway for glycerol catabolism; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family
YMR135C | [NAME] | suppressed after 8 days |  | Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains [NAME] and CTLH domains, like Vid30p; dosage-dependent regulator of START
YBR045C | GIP1 | suppressed after 8 days |  | Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p
YML006C | GIS4 | suppressed after 8 days |  | CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway
YDL171C | GLT1 | suppressed after 8 days |  | NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source
YOL059W | GPD2 | suppressed after 8 days |  | NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria
YGL121C | [NAME] | suppressed after 8 days |  | Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires [NAME] or Gpb2p to interact with Gpa2p; overproduction causes prion curing
YOL056W | GPM3 | suppressed after 8 days |  | [NAME] of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
YPL223C | [NAME] | suppressed after 8 days |  | Hydrophilin of unknown function; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway
YCL035C | GRX1 | suppressed after 8 days |  | Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair; protects cells from oxidative damage
YDL010W | GRX6 | suppressed after 8 days |  | Cis-golgi localized monothiol glutaredoxin that binds an iron-sulfur cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; functional overlap with GRX7
[NAME] | [NAME] | suppressed after 8 days |  | Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase
YLR258W | [NAME] | suppressed after 8 days |  | Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase
YHL032C | GUT1 | suppressed after 8 days |  | Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
YIL155C | GUT2 | suppressed after 8 days |  | Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner
YPR005C | HAL1 | suppressed after 8 days |  | Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p
[NAME] | [NAME] | suppressed after 8 days |  | Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
[NAME] | [NAME] | suppressed after 8 days |  | Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
[NAME] | [NAME] | suppressed after 8 days |  | Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
YNL281W | HCH1 | suppressed after 8 days |  | Heat shock protein regulator that binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus
[NAME] | [NAME] | suppressed after 8 days |  | Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation
YDR528W | HLR1 | suppressed after 8 days |  | Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A
[NAME] | HMI1 | suppressed after 8 days |  | Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
YJR147W | HMS2 | suppressed after 8 days |  | Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant
[NAME] | HNM1 | suppressed after 8 days |  | Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
YER062C | HOR2 | suppressed after 8 days |  | One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition
YEL066W | HPA3 | suppressed after 8 days |  | D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro
YIL110W | HPM1 | suppressed after 8 days |  | AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
YBR272C | [NAME] | suppressed after 8 days |  | Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b
YCR021C | HSP30 | suppressed after 8 days |  | Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
YPL240C | HSP82 | suppressed after 8 days |  | Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p
YOL068C | HST1 | suppressed after 8 days | confirmed using random sporulation | NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YOR025W | HST3 | suppressed after 8 days |  | Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
[NAME] | HUA2 | suppressed after 8 days |  | Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YHR096C | [NAME] | suppressed after 8 days |  | Hexose transporter with moderate affinity for glucose, induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs
YOR126C | IAH1 | suppressed after 8 days |  | Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing
YLR099C | ICT1 | suppressed after 8 days |  | Lysophosphatidic acid acyltransferase, responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress
YMR195W | [NAME] | suppressed after 8 days |  | Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation
YJL057C | IKS1 | suppressed after 8 days |  | Putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p
YGR287C | IMA1 | suppressed after 8 days |  | Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization; has specificity for isomaltose, palatinose, and methyl-alpha-glucoside; member of the IMA isomaltase family
YJL082W | IML2 | suppressed after 8 days |  | Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YJL037W | IRC18 | suppressed after 8 days |  | Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci
YIR036C | IRC24 | suppressed after 8 days |  | Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci
YOR155C | ISN1 | suppressed after 8 days |  | Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
YPL135W | ISU1 | suppressed after 8 days |  | Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
YBR245C | ISW1 | suppressed after 8 days |  | Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase that forms a complex with Ioc2p and Ioc4p to regulate transcription elongation, and a complex with Ioc3p to repress transcription initiation
YOR304W | ISW2 | suppressed after 8 days |  | ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, [NAME], and early meiotic genes during mitotic growth
YOL103W | ITR2 | suppressed after 8 days |  | Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively
YDR229W | IVY1 | suppressed after 8 days |  | Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase
YER051W | JHD1 | suppressed after 8 days |  | [NAME] family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, [NAME], C. elegans, and S. pombe
YJR091C | JSN1 | suppressed after 8 days |  | Member of the [NAME] family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl
YPL269W | [NAME] | suppressed after 8 days |  | Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
YPL263C | [NAME] | suppressed after 8 days |  | Cytoplasmic protein of unknown function
YPL145C | KES1 | suppressed after 8 days |  | Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex
YGL203C | KEX1 | suppressed after 8 days |  | Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
YHR082C | KSP1 | suppressed after 8 days |  | Ser/thr protein kinase; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, [NAME], and Sks1p; overproduction causes allele-specific suppression of prp20-10
YJL207C | LAA1 | suppressed after 8 days |  | AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
[NAME] | [NAME] | suppressed after 8 days |  | Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p
YLR011W | LOT6 | suppressed after 8 days |  | FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions
YPR073C | [NAME] | suppressed after 8 days |  | Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
[NAME] | [NAME] | suppressed after 8 days |  | Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
[NAME] | LYS4 | suppressed after 8 days |  | Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
YKL029C | MAE1 | suppressed after 8 days |  | Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
YCR019W | MAK32 | suppressed after 8 days |  | Protein necessary for structural stability of L-A double-stranded RNA-containing particles
YDL054C | MCH1 | suppressed after 8 days |  | Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
YOR306C | [NAME] | suppressed after 8 days |  | Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
YOL126C | MDH2 | suppressed after 8 days |  | Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
YDL078C | [NAME] | suppressed after 8 days |  | Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle
YPL270W | MDL2 | suppressed after 8 days |  | Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and [NAME] implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
YLR368W | MDM30 | suppressed after 8 days |  | F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; [NAME] degradation of Gal4p in some strains
YFR030W | MET10 | suppressed after 8 days |  | Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide
[NAME] | MET31 | suppressed after 8 days |  | Zinc-finger DNA-binding protein, involved in transcriptional regulation of the methionine biosynthetic genes, similar to Met32p
YPL140C | MKK2 | suppressed after 8 days |  | Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p
YPR188C | MLC2 | suppressed after 8 days |  | Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
YLR035C | MLH2 | suppressed after 8 days |  | Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p
YOR350C | [NAME] | suppressed after 8 days |  | Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from [NAME]
YKL064W | MNR2 | suppressed after 8 days |  | Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations
YJL066C | [NAME] | suppressed after 8 days |  | Mitochondrial membrane protein of unknown function, contains no hydrophobic stretches
YDL079C | MRK1 | suppressed after 8 days |  | Glycogen synthase kinase 3 (GSK-3) homolog; one of four [NAME] homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation
YML009C | MRPL39 | suppressed after 8 days |  | Mitochondrial ribosomal protein of the large subunit
YOR188W | MSB1 | suppressed after 8 days |  | Protein involved in positive regulation of both 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway, potential Cdc28p substrate; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4
YNL053W | MSG5 | suppressed after 8 days |  | Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates [NAME]
YNR049C | MSO1 | suppressed after 8 days |  | Probable component of the secretory vesicle docking complex, acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation
YMR023C | MSS1 | suppressed after 8 days |  | Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3
YGL051W | MST27 | suppressed after 8 days |  | Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles
YKR080W | MTD1 | suppressed after 8 days |  | NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
YGR023W | MTL1 | suppressed after 8 days |  | Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p
YPL070W | MUK1 | suppressed after 8 days |  | Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation
YBR057C | MUM2 | suppressed after 8 days |  | Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex
YKR103W | [NAME] | suppressed after 8 days |  | Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.
YDR383C | NKP1 | suppressed after 8 days |  | Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p)
YLR285W | NNT1 | suppressed after 8 days |  | S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination
YOL041C | NOP12 | suppressed after 8 days |  | Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to [NAME] and Nsr1p
YOR209C | NPT1 | suppressed after 8 days |  | Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
YGL067W | NPY1 | suppressed after 8 days |  | NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes
YNL129W | [NAME] | suppressed after 8 days |  | Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis
[NAME] | NST1 | suppressed after 8 days |  | Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor [NAME] and also displays genetic interaction with MSL1
YOL043C | [NAME] | suppressed after 8 days |  | DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus; sumoylated
[NAME] | NTH2 | suppressed after 8 days |  | Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses
YKL120W | OAC1 | suppressed after 8 days |  | Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family
YKR035C | OPI8 | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
YJL212C | OPT1 | suppressed after 8 days |  | Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
YOR085W | OST3 | suppressed after 8 days |  | Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
YMR174C | PAI3 | suppressed after 8 days |  | Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
YBR301W | PAU24 | suppressed after 8 days |  | Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
YFL020C | PAU5 | suppressed after 8 days |  | Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme
YAR020C | PAU7 | suppressed after 8 days |  | Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
YIL071C | PCI8 | suppressed after 8 days |  | Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
YNL289W | PCL1 | suppressed after 8 days |  | Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth
YOR328W | PDR10 | suppressed after 8 days |  | ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p
YBL005W | PDR3 | suppressed after 8 days |  | Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome
YOR153W | PDR5 | suppressed after 8 days |  | Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth
YGR193C | PDX1 | suppressed after 8 days |  | Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
YCR020C | PET18 | suppressed after 8 days |  | Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
[NAME] | [NAME] | suppressed after 8 days |  | Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly
YJL179W | PFD1 | suppressed after 8 days |  | Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin
YKL127W | PGM1 | suppressed after 8 days |  | Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism
YMR105C | PGM2 | suppressed after 8 days |  | Phosphoglucomutase, catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase
[NAME] | PHA2 | suppressed after 8 days |  | Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
YBR296C | PHO89 | suppressed after 8 days |  | Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p
[NAME] | PIG1 | suppressed after 8 days |  | Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase
YHR034C | PIH1 | suppressed after 8 days |  | Protein of unresolved function; may function in protein folding and/or rRNA processing, interacts with a chaperone (Hsp82p), two chromatin remodeling factors (Rvb1p, [NAME]) and two rRNA processing factors (Rrp43p, Nop58p)
YOR363C | PIP2 | suppressed after 8 days |  | Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes
YMR008C | PLB1 | suppressed after 8 days |  | Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol
YDR183W | PLP1 | suppressed after 8 days |  | Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
YOR161C | PNS1 | suppressed after 8 days |  | Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
YOR266W | PNT1 | suppressed after 8 days |  | Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
YMR129W | POM152 | suppressed after 8 days |  | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase;
YIL114C | POR2 | suppressed after 8 days |  | Putative mitochondrial porin (voltage-dependent anion channel), related to Por1p but not required for mitochondrial membrane permeability or mitochondrial osmotic stability
YOL141W | PPM2 | suppressed after 8 days |  | AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
YAR031W | PRM9 | suppressed after 8 days |  | Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family
YKL116C | PRR1 | suppressed after 8 days |  | Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor
YOL061W | PRS5 | suppressed after 8 days |  | 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
YMR137C | PSO2 | suppressed after 8 days |  | Nuclease required for a post-incision step in the repair of DNA single and double-strand breaks that result from interstrand crosslinks produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation
[NAME] | [NAME] | suppressed after 8 days |  | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
YBL056W | PTC3 | suppressed after 8 days |  | Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation
YOR208W | PTP2 | suppressed after 8 days |  | Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus
YER075C | PTP3 | suppressed after 8 days |  | Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm
YPR042C | PUF2 | suppressed after 8 days |  | Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer [NAME] binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YLR142W | PUT1 | suppressed after 8 days |  | Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
YPL147W | PXA1 | suppressed after 8 days |  | Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins
YOR347C | PYK2 | suppressed after 8 days |  | Pyruvate kinase that appears to be modulated by phosphorylation; PYK2 transcription is repressed by glucose, and Pyk2p may be active under low glycolytic flux
YFR033C | QCR6 | suppressed after 8 days |  | Subunit 6 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1
YGR183C | [NAME] | suppressed after 8 days |  | Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
[NAME] | [NAME] | suppressed after 8 days |  | Multidrug transporter of the major facilitator superfamily, has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
YML095C | RAD10 | suppressed after 8 days |  | Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
YMR201C | [NAME] | suppressed after 8 days |  | Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
YBR114W | RAD16 | suppressed after 8 days |  | Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
YLR032W | RAD5 | suppressed after 8 days |  | DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p
[NAME] | RAD59 | suppressed after 8 days |  | Protein involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; homologous to Rad52p
YLR084C | RAX2 | suppressed after 8 days |  | N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; [NAME] stability is regulated by Mpt5p
YPL246C | RBD2 | suppressed after 8 days |  | Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
YAL036C | RBG1 | suppressed after 8 days |  | Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with [NAME], Ygr250cp, Gir2p and Yap1p via two-hybrid
YLR248W | RCK2 | suppressed after 8 days |  | Protein kinase involved in the response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to [NAME] (calmodulin) kinases
YKL159C | RCN1 | suppressed after 8 days |  | Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region
YBR073W | RDH54 | suppressed after 8 days |  | DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference
YJR021C | REC107 | suppressed after 8 days |  | Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex ([NAME]) required for ds break formation
YCL001W | RER1 | suppressed after 8 days |  | Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER
[NAME] | REX2 | suppressed after 8 days |  | 3'-5' [NAME] exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease
YLR107W | [NAME] | suppressed after 8 days |  | RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with [NAME] and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases
YKL038W | RGT1 | suppressed after 8 days |  | Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor
YKR055W | RHO4 | suppressed after 8 days |  | Non-essential small GTPase of the Rho/Rac subfamily of [NAME]-like proteins, likely to be involved in the establishment of cell polarity
YPL089C | RLM1 | suppressed after 8 days |  | MADS-box transcription factor, component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p
YGR044C | RME1 | suppressed after 8 days |  | Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by [NAME] regulator; mediates cell type control of sporulation
YBR229C | ROT2 | suppressed after 8 days |  | Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
YNL301C | RPL18B | suppressed after 8 days |  | Protein component of the large (60S) ribosomal subunit, identical to Rpl18Ap and has similarity to rat L18 ribosomal protein
YFL034C-A | RPL22B | suppressed after 8 days |  | Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Ap and to rat L22 ribosomal protein
YGL031C | RPL24A | suppressed after 8 days |  | Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate
YDL081C | RPP1A | suppressed after 8 days |  | Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
YDR382W | RPP2B | suppressed after 8 days |  | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
YCR031C | RPS14A | suppressed after 8 days |  | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins
YJL191W | RPS14B | suppressed after 8 days |  | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins
[NAME] | RPS16B | suppressed after 8 days |  | Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosomal proteins
YER074W | RPS24A | suppressed after 8 days |  | Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein
YOR167C | [NAME] | suppressed after 8 days |  | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Bp and has similarity to rat S28 ribosomal protein
YNL096C | RPS7B | suppressed after 8 days |  | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and [NAME] ribosomal proteins
YOL117W | RRI2 | suppressed after 8 days |  | Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
YHR031C | RRM3 | suppressed after 8 days |  | DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p
YDR083W | RRP8 | suppressed after 8 days |  | Nucleolar protein involved in rRNA processing, pre-rRNA cleavage at site A2; also involved in telomere maintenance; mutation is synthetically lethal with a gar1 mutation
YOR049C | [NAME] | suppressed after 8 days |  | Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
YJR127C | RSF2 | suppressed after 8 days |  | Zinc-finger protein involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions
YGR152C | RSR1 | suppressed after 8 days |  | GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases
[NAME] | SAL1 | suppressed after 8 days |  | ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains
[NAME] | SAM3 | suppressed after 8 days |  | High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
YDR143C | SAN1 | suppressed after 8 days |  | Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition
[NAME] | SAS3 | suppressed after 8 days |  | Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal
YDR351W | SBE2 | suppressed after 8 days |  | Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
YMR214W | SCJ1 | suppressed after 8 days |  | One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with [NAME] to mediate maturation of proteins
YGL126W | SCS3 | suppressed after 8 days |  | Protein required for inositol prototrophy, identified as an ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis; disputed role in the synthesis of inositol phospholipids from inositol
YMR305C | SCW10 | suppressed after 8 days |  | Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p
YGR279C | SCW4 | suppressed after 8 days |  | Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating
YLR292C | SEC72 | suppressed after 8 days |  | Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YOR184W | SER1 | suppressed after 8 days |  | 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p
YGR208W | SER2 | suppressed after 8 days |  | Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
YJL168C | SET2 | suppressed after 8 days | confirmed using tetrad analysis | Histone methyltransferase with a role in transcriptional elongation, methylates a lysine residue of histone H3; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
YPL165C | SET6 | suppressed after 8 days |  | SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
YJR095W | SFC1 | suppressed after 8 days |  | Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization
YOR140W | SFL1 | suppressed after 8 days |  | Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p
YGL066W | SGF73 | suppressed after 8 days |  | SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
YER096W | SHC1 | suppressed after 8 days |  | Sporulation-specific activator of Chs3p (chitin synthase III), required for the synthesis of the chitosan layer of ascospores; has similarity to Skt5p, which activates Chs3p during vegetative growth; transcriptionally induced at alkaline pH
YLR058C | SHM2 | suppressed after 8 days |  | Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
YOL110W | SHR5 | suppressed after 8 days |  | Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization
YOL031C | SIL1 | suppressed after 8 days |  | Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein
YEL065W | SIT1 | suppressed after 8 days |  | Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
YNL032W | SIW14 | suppressed after 8 days |  | Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
YOR076C | SKI7 | suppressed after 8 days |  | Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype
YGR143W | SKN1 | suppressed after 8 days |  | Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p
YBL061C | SKT5 | suppressed after 8 days |  | Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation
YBR266C | SLM6 | suppressed after 8 days |  | Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase
YLR135W | SLX4 | suppressed after 8 days |  | Endonuclease involved in processing DNA during recombination and repair; cleaves branched structures in a complex with Slx1p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing
YOR307C | SLY41 | suppressed after 8 days |  | Protein involved in ER-to-Golgi transport
YDR525W-A | [NAME] | suppressed after 8 days |  | Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YDR073W | SNF11 | suppressed after 8 days |  | Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p
YGR197C | SNG1 | suppressed after 8 days |  | Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance
[NAME] | [NAME] | suppressed after 8 days |  | Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
YDR011W | SNQ2 | suppressed after 8 days |  | Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
[NAME] | SNX3 | suppressed after 8 days |  | Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
YGL127C | SOH1 | suppressed after 8 days |  | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan [NAME] subunits
YNR034W | SOL1 | suppressed after 8 days |  | Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol2p, Sol3p, and Sol4p
[NAME] | SOP4 | suppressed after 8 days |  | ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER
YKL184W | SPE1 | suppressed after 8 days |  | Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
YMR107W | SPG4 | suppressed after 8 days |  | Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
YIL073C | SPO22 | suppressed after 8 days |  | Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis
YPR032W | SRO7 | suppressed after 8 days |  | Effector of Rab GTPase Sec4p, forms a complex with [NAME] and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro77p and Drosophila lgl tumor suppressor
YMR101C | SRT1 | suppressed after 8 days |  | Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase
[NAME] | SRX1 | suppressed after 8 days |  | Sulfiredoxin, contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes
YLL024C | SSA2 | suppressed after 8 days |  | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes
YDR293C | [NAME] | suppressed after 8 days |  | Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YNR031C | SSK2 | suppressed after 8 days |  | MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
YOR047C | STD1 | suppressed after 8 days |  | Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]
[NAME] | STF2 | suppressed after 8 days |  | Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p may act as stabilizing factors that enhance inhibitory action of the Inh1p protein
YDL048C | STP4 | suppressed after 8 days |  | Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor
[NAME] | SUC2 | suppressed after 8 days |  | Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively
YHR181W | SVP26 | suppressed after 8 days |  | Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment
YPL163C | SVS1 | suppressed after 8 days |  | Cell wall and vacuolar protein, required for wild-type resistance to vanadate
YLR385C | SWC7 | suppressed after 8 days |  | Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin
YDR126W | SWF1 | suppressed after 8 days |  | Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
[NAME] | SYP1 | suppressed after 8 days |  | Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization
YGL243W | TAD1 | suppressed after 8 days |  | tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala
YGL232W | TAN1 | suppressed after 8 days |  | Putative tRNA acetyltransferase, RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA
[NAME] | TCB1 | suppressed after 8 days |  | Lipid-binding ER protein, enriched at ER-plasma membrane contact sites; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
YJR009C | TDH2 | suppressed after 8 days |  | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall
[NAME] | TES1 | suppressed after 8 days |  | Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
YLR178C | TFS1 | suppressed after 8 days |  | Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase
YKL140W | [NAME] | suppressed after 8 days |  | [NAME] ester hydrolase, one of three gene products (Yeh1p, Yeh2p, [NAME]) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
YKR089C | TGL4 | suppressed after 8 days |  | Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p
YNL139C | THO2 | suppressed after 8 days | confirmed using random sporulation | Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
YGL049C | TIF4632 | suppressed after 8 days |  | Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains [NAME] ([NAME]); associates with the poly(A)-binding protein [NAME], also interacts with eIF4A (Tif1p); homologous to Tif4631p
YGR181W | TIM13 | suppressed after 8 days |  | Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YOR010C | TIR2 | suppressed after 8 days |  | Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
YKR010C | [NAME] | suppressed after 8 days |  | Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site
YLR234W | TOP3 | suppressed after 8 days |  | DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination
YNL079C | TPM1 | suppressed after 8 days |  | Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently
YOL093W | TRM10 | suppressed after 8 days |  | tRNA methyltransferase, methylates the N-1 position of guanosine in tRNAs
YML005W | TRM12 | suppressed after 8 days |  | S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA
YDR354W | TRP4 | suppressed after 8 days |  | Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YOR115C | TRS33 | suppressed after 8 days |  | One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YOR344C | TYE7 | suppressed after 8 days |  | Serine-rich protein that contains a basic-helix-loop-helix ([NAME]) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression
[NAME] | UBC5 | suppressed after 8 days |  | Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible
YKR098C | UBP11 | suppressed after 8 days |  | Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins
YIL156W | UBP7 | suppressed after 8 days |  | Ubiquitin-specific protease that cleaves ubiquitin-protein fusions
YKL010C | [NAME] | suppressed after 8 days |  | Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins
YBR006W | UGA2 | suppressed after 8 days |  | Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YDR207C | UME6 | suppressed after 8 days |  | Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p
YML106W | URA5 | suppressed after 8 days |  | Major orotate phosphoribosyltransferase (OPRTase) isozyme that catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; minor OPRTase encoded by URA10
YDL077C | VAM6 | suppressed after 8 days |  | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
YDL128W | VCX1 | suppressed after 8 days |  | Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
YPL234C | VMA11 | suppressed after 8 days |  | Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen
YEL027W | VMA3 | suppressed after 8 days |  | Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis
YPL019C | VTC3 | suppressed after 8 days |  | Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion
YIL173W | VTH1 | suppressed after 8 days |  | Putative membrane glycoprotein with strong similarity to [NAME] and Pep1p/Vps10p, may be involved in vacuolar protein sorting
YML076C | WAR1 | suppressed after 8 days |  | [NAME] finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and [NAME], encoding an acid transporter and a putative ammonia transporter, respectively
[NAME] | WSC2 | suppressed after 8 days |  | Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YAR035W | YAT1 | suppressed after 8 days |  | Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
YBR216C | [NAME] | suppressed after 8 days |  | Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress
YGL060W | YBP2 | suppressed after 8 days |  | Central kinetochore associated protein that mediates mitotic progression; interacts with several central kinetochore proteins and the centromeric histone Cse4p; role in resistance to oxidative stress; similarity to Ybp1p and Slk19p
YCR004C | YCP4 | suppressed after 8 days |  | Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR020C | YEH2 | suppressed after 8 days |  | [NAME] ester hydrolase, [NAME] ester hydrolysis at the plasma membrane; involved in sterol metabolism
YHR029C | YHI9 | suppressed after 8 days |  | Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production
[NAME] | YHM2 | suppressed after 8 days |  | Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
YIL006W | [NAME] | suppressed after 8 days |  | Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs
YKL067W | [NAME] | suppressed after 8 days |  | Nucleoside diphosphate kinase, catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate
YDR057W | [NAME] | suppressed after 8 days |  | ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family
YMR104C | YPK2 | suppressed after 8 days |  | Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with [NAME] at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK
YHR105W | YPT35 | suppressed after 8 days |  | Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport
YKR014C | YPT52 | suppressed after 8 days |  | Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis
YHR017W | YSC83 | suppressed after 8 days |  | Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
YJL139C | [NAME] | suppressed after 8 days |  | Mannosyltransferase of the KTR1 family, involved in protein N-glycosylation; located in the Golgi apparatus
YOL109W | [NAME] | suppressed after 8 days |  | Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria
YLR057W | MNL2 | suppressed after 8 days |  | Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and [NAME], that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
YGL226W | MTC3 | suppressed after 8 days |  | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
YHL029C | OCA5 | suppressed after 8 days |  | Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
YHL046C | PAU13 | suppressed after 8 days |  | Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock
YLL023C | POM33 | suppressed after 8 days |  | [NAME] nucleoporin involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p
YER067W | RGI1 | suppressed after 8 days |  | Protein of unknown function involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion
YDR198C | RKM2 | suppressed after 8 days |  | Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp
YLR046C | RLA1 | suppressed after 8 days |  | Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
YER066W | RRT13 | suppressed after 8 days |  | Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
YNL105W | RRT16 | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
YBR147W | RTC2 | suppressed after 8 days |  | Protein of unknown function; mutant produces large lipid droplets, is resistant to fluconazole, has decreased levels of rDNA transcription, growth defects on minimal media, and suppresses cdc13-1; detected in highly purified mitochondria; similar to a G-protein coupled receptor from S. pombe
YDL025C | [NAME] | suppressed after 8 days |  | Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and [NAME]
[NAME] | RTT10 | suppressed after 8 days |  | WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; has a role in regulation of Ty1 transposition; human ortholog is WDR6
[NAME] | SAF1 | suppressed after 8 days |  | F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1
YGR263C | [NAME] | suppressed after 8 days |  | Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
YBL104C | SEA4 | suppressed after 8 days |  | Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites
YIL064W | SEE1 | suppressed after 8 days |  | Probable lysine methyltransferase involved in the dimethylation of [NAME] (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
YLR426W | TDA5 | suppressed after 8 days |  | Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YLR004C | THI73 | suppressed after 8 days |  | Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs
[NAME] | TRI1 | suppressed after 8 days |  | Non-essential sumoylated protein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus
[NAME] | TRM13 | suppressed after 8 days |  | 2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases
YPL030W | TRM44 | suppressed after 8 days |  | tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
YDR119W | VBA4 | suppressed after 8 days |  | Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
YKR105C | VBA5 | suppressed after 8 days |  | Putative transporter of the Major Facilitator Superfamily (MFS); proposed role as a basic amino acid permease based on phylogeny
YNL321W | [NAME] | suppressed after 8 days |  | Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate
YKL069W | YKG9 | suppressed after 8 days |  | [NAME] reductase, reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress
YBR028C | [NAME] | suppressed after 8 days |  | An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner
YPR053C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C
YGL117W |  | suppressed after 8 days |  | Putative protein of unknown function
YDR215C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein
YMR244W |  | suppressed after 8 days |  | Putative protein of unknown function
YJL215C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR111C |  | suppressed after 8 days |  | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene
YOR366W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; expression is increased greatly during sporulation; [NAME] is not an essential gene
YFR035C |  | suppressed after 8 days |  | Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein
YIL165C |  | suppressed after 8 days |  | Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene
YKL123W |  | suppressed after 8 days |  | Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4
YNL285W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR015C |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
YNR021W |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; [NAME] is not an essential gene
YBR113W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8
YLR281C |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; [NAME] is not an essential gene
YCR023C |  | suppressed after 8 days |  | Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
YLR287C |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene
YHL042W |  | suppressed after 8 days |  | Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
YJR107W |  | suppressed after 8 days |  | Putative protein of unknown function; has sequence or structural similarity to lipases
YIL024C |  | suppressed after 8 days |  | Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p
[NAME] |  | suppressed after 8 days |  | Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; [NAME] is not an essential gene
YDR220C |  | suppressed after 8 days |  | Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein
YDR222W |  | suppressed after 8 days |  | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YPL108W |  | suppressed after 8 days |  | Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YMR178W |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR178W is not an essential gene
[NAME] |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; [NAME] is not an essential gene.
YBL096C |  | suppressed after 8 days |  | Non-essential protein of unknown function
[NAME] |  | suppressed after 8 days |  | Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene
YMR124W |  | suppressed after 8 days |  | Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
YML020W |  | suppressed after 8 days |  | Putative protein of unknown function
[NAME] |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
YOR012W |  | suppressed after 8 days |  | Putative protein of unknown function
YPL257W |  | suppressed after 8 days |  | Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene
YLR149C |  | suppressed after 8 days |  | Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; [NAME] is not an essential gene
YPL185W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C
YCR100C |  | suppressed after 8 days |  | Putative protein of unknown function
YKL066W |  | suppressed after 8 days |  | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene [NAME]
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family
YLR290C |  | suppressed after 8 days |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene
YDR010C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR365W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene
[NAME] |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A
YLR294C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14
YBR056W |  | suppressed after 8 days |  | Putative cytoplasmic protein of unknown function
YGL262W |  | suppressed after 8 days |  | Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; [NAME] is not an essential gene
YNL089C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of [NAME]-like proteins
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; [NAME] is not an essential gene
YMR316C-B |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOL037C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W
YNL058C |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene
YMR206W |  | suppressed after 8 days |  | Putative protein of unknown function; YMR206W is not an essential gene
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
YPL264C |  | suppressed after 8 days |  | Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene
YER079W |  | suppressed after 8 days |  | Putative protein of unknown function
YKL075C |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
[NAME] |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
YBR209W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene
YHR162W |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
YBR064W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C
YBR139W |  | suppressed after 8 days |  | Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
YKR032W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR012C |  | suppressed after 8 days |  | Putative protein of unknown function; YLR012C is not an essential gene
YNL092W |  | suppressed after 8 days |  | [NAME]-dependent methyltransferase of the seven beta-strand family; [NAME] is not an essential gene
YCR006C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR209C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W.
YER188W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p
YGR042W |  | suppressed after 8 days |  | Putative protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YHR022C |  | suppressed after 8 days |  | Putative protein of unknown function; YHR022C is not an essential gene
[NAME] |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; [NAME] is not an essential gene.
YFL034W |  | suppressed after 8 days |  | Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
YJL175W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor
YMR074C |  | suppressed after 8 days |  | Protein with homology to human PDCD5, which is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene
YIR007W |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene
YPR014C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YMR245W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR455W |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YDR102C |  | suppressed after 8 days |  | Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index
YGL235W |  | suppressed after 8 days |  | Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and [NAME]
YCL022C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W
YBL044W |  | suppressed after 8 days |  | Putative protein of unknown function; YBL044W is not an essential protein
YKR040C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF [NAME]
[NAME] |  | suppressed after 8 days |  | Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; [NAME] is not an essential gene
YNL319W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14
YJL107C |  | suppressed after 8 days |  | Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
YPR096C |  | suppressed after 8 days |  | Protein of unknown function that may interact with ribosomes, based on co-purification experiments
YAR023C |  | suppressed after 8 days |  | Putative integral membrane protein, member of DUP240 gene family
YDR541C |  | suppressed after 8 days |  | Putative dihydrokaempferol 4-reductase
YAR029W |  | suppressed after 8 days |  | Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YDL086W |  | suppressed after 8 days |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; [NAME] is not an essential gene
YNR063W |  | suppressed after 8 days |  | Putative zinc-cluster protein of unknown function
YMR086W |  | suppressed after 8 days |  | Component of the eisosome with unknown function; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cell periphery; expression is repressed by cAMP; similar to A. gossypii SEG gene which is important for stabilizing eisosomes
YNL034W |  | suppressed after 8 days |  | Putative protein of unknown function; [NAME] is not an essential gene
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function with similarity to proteins containing WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; [NAME] is not an essential gene
YLR194C |  | suppressed after 8 days |  | Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress
YPR027C |  | suppressed after 8 days |  | Putative protein of unknown function
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YHR180W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR042C |  | suppressed after 8 days |  | Putative protein of unknown function; expression is increased in ssu72-ts69 mutant
YDL012C |  | suppressed after 8 days |  | Tail-anchored plasma membrane protein containing a conserved CYSTM module, possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype
YNL324W |  | suppressed after 8 days |  | Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR010W |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; [NAME] is transcribed with [NAME]
YLR036C |  | suppressed after 8 days |  | Putative protein of unknown function predicted to have transmembrane domains; interacts with [NAME] by yeast two-hybrid analysis; YLR036C is not an essential protein
YKL027W |  | suppressed after 8 days |  | Protein of unknown function, localized to the mitochondrial outer membrane
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; [NAME] is a non-essential gene
YOL098C |  | suppressed after 8 days |  | Putative metalloprotease
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p
YGL034C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR139W |  | suppressed after 8 days |  | Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL109W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene [NAME]
YKL177W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene STE3
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles
YOL024W |  | suppressed after 8 days |  | Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site
YBR238C |  | suppressed after 8 days |  | Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
YOR342C |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus
YDR124W |  | suppressed after 8 days |  | Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor
YBL062W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJL120W |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
YCR007C |  | suppressed after 8 days |  | Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene
[NAME] |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
[NAME] |  | suppressed after 8 days |  | Putative protein of unknown function
YDL026W |  | suppressed after 8 days |  | Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YFR018C |  | suppressed after 8 days |  | Putative protein of unknown function
YPL080C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR271C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
YEL008W |  | suppressed after 8 days |  | Hypothetical protein predicted to be involved in metabolism
YJL055W |  | suppressed after 8 days |  | Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU
YJR087W |  | suppressed after 8 days |  | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and [NAME]
YIL077C |  | suppressed after 8 days |  | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YMR027W |  | suppressed after 8 days |  | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene
YLR334C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here
YNR014W |  | suppressed after 8 days |  | Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible
YML100W-A |  | suppressed after 8 days |  | Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YER038W-A |  | suppressed after 8 days |  | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria
YGL041C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR416C |  | suppressed after 8 days |  | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
[NAME] |  | suppressed after 8 days |  | Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
YDL186W |  | suppressed after 8 days |  | Putative protein of unknown function; YDL186W is not an essential gene
YPR089W |  | suppressed after 8 days |  | Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p
YOL013W-A |  | suppressed after 8 days |  | Putative protein of unknown function; identified by SAGE
YIR044C |  | suppressed after 8 days |  | Possible pseudogene in strain S288C; [NAME] and the adjacent ORF, [NAME], together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
YCL075W |  | suppressed after 8 days |  | [NAME]: encodes fragment of [NAME] protein