Spreadsheet: 37df2cbc-030d-44a1-af1c-0ee46ea66f21
Sheet: Sheet1

Table S3: Age-dependent aggregation-prone proteins identified as significantly regulated in daf-2(e1370) versus daf-16(mu86); daf-2(e1370) microarrays (Shaw et al. 2007) | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7
Uniprot ID | Gene WB ID | Gene name | Sequence name | # Sig. Probes | % Probe sign agreement | Average fold change | Protein name
O16226 | [NAME] | C49G7.3 | C49G7.3 | 2 | 1 | 0.2525799089637966 | Putative uncharacterized protein
Q22038 | WBGene00004357 | rho-1 | Y51H4A.3 | 1 | 1 | 0.2855591631205323 | Ras-like GTP-binding protein rhoA
O01761 | WBGene00006820 | unc-89 | C09D1.1 | 2 | 1 | 0.4555078206252541 | Muscle M-line assembly protein unc-89
Q9N358 | WBGene00021934 | Y55F3AR.3 | Y55F3AR.3 | 2 | 1 | 0.4673334928724696 | T-complex protein 1 subunit theta
P91374 | WBGene00004427 | rpl-15 | [NAME] | 2 | 1 | 0.4940348373871485 | 60S ribosomal protein L15
P34334 | WBGene00004433 | rpl-21 | C14B9.7 | 2 | 1 | 0.5050436484799805 | 60S ribosomal protein [NAME]
P11141 | WBGene00002010 | hsp-6 | C37H5.8 | 2 | 1 | 0.5115945856777981 | Heat shock 70 kDa protein F, mitochondrial
Q19877 | WBGene00004492 | rps-23 | F28D1.7 | 2 | 1 | 0.5397042252103273 | 40S ribosomal protein S23
Q9NLD1 | WBGene00002000 | hrp-2 | F58D5.1 | 2 | 1 | 0.5456386530189316 | Protein F58D5.1a
Q9BKU3 | WBGene00021351 | Y37E3.10 | Y37E3.10 | 2 | 1 | 0.5646973802408238 | Putative uncharacterized protein
O61955 | WBGene00002061 | ife-3 | B0348.6 | 4 | 1 | 0.569627185908249 | Eukaryotic translation initiation factor 4E-3
Q19286 | WBGene00002054 | ifb-2 | F10C1.7 | 4 | 1 | 0.5861543251124017 | Intermediate filament protein ifb-2
Q23315 | WBGene00001337 | ers-2 | ZC434.5 | 2 | 1 | 0.5939175908511036 | Protein ZC434.5
Q22099 | WBGene00002238 | krs-1 | T02G5.9 | 2 | 1 | 0.6031545790106094 | Lysyl-tRNA synthetase
P48158 | WBGene00004435 | rpl-23 | B0336.10 | 1 | 1 | 0.605066647978988 | 60S ribosomal protein [NAME]
Q21351 | WBGene00010677 | K08F4.2 | K08F4.2 | 2 | 1 | 0.6234891936444706 | [NAME]
[NAME] | WBGene00004418 | rpl-7 | F53G12.10 | 2 | 1 | 0.6280172293973338 | 60S ribosomal protein L7
Q7K7B6 | WBGene00006876 | vab-10 | ZK1151.1 | 2 | 1 | 0.6338517016172308 | Protein ZK1151.1b
Q6EUT7 | WBGene00006876 | vab-10 | ZK1151.1 | 2 | 1 | 0.6338517016172308 | [NAME]
Q09581 | WBGene00002266 | lec-3 | ZK892.1 | 6 | 1 | 0.6505285603125716 | 32 [NAME] beta-galactoside-binding lectin lec-3
P14792 | WBGene00006728 | ubq-1 | ZK1010.1 | 2 | 1 | 0.65554689005562 | Ubiquitin
Q966C6 | WBGene00004419 | rpl-7A | Y24D9A.4 | 4 | 1 | 0.662112393369617 | 60S ribosomal protein L7a
Q20684 | WBGene00002265 | lec-2 | F52H3.7 | 4 | 1 | 0.6691222031607112 | Protein F52H3.7a
Q20655 | WBGene00001502 | ftt-2 | F52D10.3 | 4 | 1 | 0.6783602699825985 | 14-3-3-like protein 2
Q95ZT1 | WBGene00001502 | ftt-2 | F52D10.3 | 4 | 1 | 0.6783602699825985 | Protein F52D10.3b
O76840 | WBGene00016498 | ppn-1 | C37C3.6 | 3 | 1 | 0.6810343035279709 | Papilin
O02141 | WBGene00016722 | C46G7.2 | C46G7.2 | 2 | 1 | 0.6829417917991312 | Putative uncharacterized protein
O18673 | WBGene00001488 | frm-1 | ZK270.2 | 4 | 1 | 0.6908504977995751 | [NAME]
[NAME] | WBGene00002978 | lev-11 | Y105E8B.1 | 2 | 1 | 0.6922906886909715 | Tropomyosin isoforms a/b/d/f
O18240 | [NAME] | rps-18 | Y57G11C.16 | 2 | 1 | 0.7335647895595487 | Protein Y57G11C.16
A7DT47 | WBGene00004130 | [NAME] | F54E2.3 | 4 | 0.75 | 0.7473289554704381 | [NAME] (Drosophila actin-binding) homolog protein 1, isoform a
O17338 | WBGene00020732 | T23E7.2 | T23E7.2 | 8 | 0.875 | 0.8115005796011666 | Putative uncharacterized protein
Q9TXR9 | WBGene00020732 | T23E7.2 | T23E7.2 | 8 | 0.875 | 0.8115005796011666 | Putative uncharacterized protein
B6VQ97 | WBGene00006787 | unc-52 | ZC101.2 | 4 | 0.5 | 0.8513449226612346 | Protein ZC101.2d
P91128 | [NAME] | rpl-13 | C32E8.2 | 4 | 0.5 | 0.8806236131980063 | 60S ribosomal protein [NAME]
Q27527 | WBGene00011884 | enol-1 | T21B10.2 | 4 | 0.5 | 1.117895652282194 | Enolase
Q23081 | WBGene00022500 | lfi-1 | ZC8.4 | 3 | 0.6666666666666666 | 1.203095748093121 | Lin-5 (Five) interacting protein protein 1, isoform a
[NAME] | WBGene00022500 | lfi-1 | ZC8.4 | 3 | 0.6666666666666666 | 1.203095748093121 | Lin-5 (Five) interacting protein protein 1, isoform d
P34552 | WBGene00007010 | alx-1 | R10E12.1 | 4 | 1 | 1.329246124035233 | Apoptosis-linked gene 2-interacting protein X 1
Q09EF5 | WBGene00018051 | dyf-14 | F35D11.11 | 4 | 1 | 1.389200967125658 | Abnormal dye filling protein 14, isoform a  (Fragment)
O62053 | WBGene00007458 | C08F11.11 | C08F11.11 | 2 | 1 | 1.451968822895382 | UPF0375 protein C08F11.11
O02143 | WBGene00004112 | pqn-22 | C46G7.4 | 2 | 1 | 1.511731075731115 | Prion-like-(Q/n-rich)-domain-bearing protein protein 22, isoform a
[NAME] | WBGene00002023 | hsp-25 | C09B8.6 | 2 | 1 | 1.533818202658401 | Heat shock protein protein 25, isoform a
Q18625 | WBGene00002267 | lec-4 | C44F1.3 | 2 | 1 | 1.544466320961382 | [NAME]
Q20330 | WBGene00018350 | F42C5.10 | F42C5.10 | 2 | 1 | 1.545191817005454 | Putative uncharacterized protein
O45786 | WBGene00011735 | T12D8.8 | T12D8.8 | 2 | 1 | 1.563362703393436 | Protein T12D8.8
Q23258 | WBGene00013867 | ZC373.2 | ZC373.2 | 2 | 1 | 1.643098751356494 | Protein ZC373.2
O62390 | WBGene00012185 | W01F3.2 | W01F3.2 | 2 | 1 | 1.689958614034435 | Protein W01F3.2
O62289 | WBGene00010440 | JC8.8 | JC8.8 | 2 | 1 | 1.745477032465269 | Protein JC8.8
P46563 | WBGene00017166 | F01F1.12 | F01F1.12 | 4 | 1 | 1.798335874646324 | Fructose-bisphosphate aldolase 2
[NAME] | WBGene00012878 | Y45F10C.2 | Y45F10C.2 | 2 | 1 | 1.858345199510697 | UPF0375 protein Y45F10C.2
Q9XWU9 | WBGene00012557 | Y37D8A.19 | Y37D8A.19 | 2 | 1 | 2.007867921170956 | Protein Y37D8A.19
Q94215 | WBGene00002274 | lec-11 | F38A5.3 | 4 | 1 | 2.164584701294048 | Galectin LEC-11
Q9TYY2 | WBGene00000535 | cpi-1 | K08B4.6 | 2 | 1 | 2.286638114796779 | [NAME]
O16202 | WBGene00003096 | lys-7 | C02A12.4 | 2 | 1 | 2.297283038197131 | Lysozyme protein 7
Q17625 | WBGene00007295 | C04C11.1 | C04C11.1 | 2 | 1 | 2.331015559803962 | Protein C04C11.1a
Q95ZL8 | WBGene00006803 | [NAME] | [NAME] | 2 | 1 | 2.391073829346755 | Putative uncharacterized protein
O16319 | WBGene00015671 | C10B5.3 | C10B5.3 | 2 | 1 | 2.803195357295764 | Putative uncharacterized protein
Note: Microarray data from Shaw et al. comparing [NAME]) versus daf-16(mu86); daf-2(e1370) identified 1570 genes up-regulated and 796 down-regulated
in daf-2 mutants (q<0.1, 17965 genes in total). The mRNA levels of 457 age-dependent aggregation-prone proteins were evaluated in these microarrays.
We note that mRNA levels of rho-1 were down-regulated in daf-2(-) mutants but the protein levels of RHO-1 remained unchanged as detected by western blot
possibly reflecting the variation between mRNA and protein levels as previously described in Gygi et al. (1999) [NAME] Biol 19: 1720-1730.
 | All Genes | Expected number | Actual number
 |  | of aggregation- | of aggregation-
 |  | prone proteins | prone proteins
Up | 1570 | 40 | 23
Not changed | 15599 | 397 | 399
Down | 796 | 20 | 35
Total | 17965 | 457 | 457
Chi-test | p-value = 0.0002