Spreadsheet: 035fb97a-3c1a-4d1a-bef9-da581a02653f
Sheet: Sheet1

BSL2 Laboratory Check List - NIH Guidelines & BMBL Condensed Version
Principal [NAME]:
Date of Visit:
Technical Representative:
Laboratory Room Number(s):
Pricipal Investigator's Phone:                                                                Lab Contact:                                                                Contact Phone:
Protocol #  IBC:                                                                                           IACUC:
Agents:
THIS DOCUMENT IS CONFIDENTIAL AND [NAME] WHEN INFORMATION IS ENTERED
Reference | Statement | Response |  |  | Comments
 |  | Yes | No | N/A
Combined NIH Recombinant DNA and BMBL REQUIREMENTS For BSL2
A | Administration
NIH: G-II:B-1-a NIH: G-II:B-2-b NIH: G-II:B-2-c BMBL: A1 BMBL: B1 | The PI establishes policies and procedures consistent with institutional policies that limit access to the laboratory or animal rooms to persons who have been advised of the potential hazard and meet any specific entry requirements (e.g. immunization).  The PI has final responsibility for assessing each circumstance and determining who may enter or work in the laboratory; access to the laboratory is limited or restricted by the PI when work with organisms containing recombinant DNA molecules is in progress; and the laboratory supervisor must enforce the institutional policies that control access to the laboratory.
NIH: G-II: B-1-h | Experiments of lesser biohazard potential can be conducted concurrently in carefully demarcated areas of the same laboratory.
BMBL: A11 NIH: G-II: B-2-m | The laboratory supervisor must ensure that laboratory personnel receive appropriate training regarding their duties, the necessary precautions to prevent exposures, and exposure evaluation procedures. Personnel are advised of special hazards and are required to read and follow instructions on practices and procedures.  Personnel must receive annual updates or additional training when procedural or policy changes occur.
BMBL: B5 | The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with BSL-2 agents.
BMBL: A11 | All laboratory personnel, particularly women of child-bearing age, should be provided with information regarding immune competence and conditions that may predispose them to infection.  Individuals having these conditions should be encouraged to self-identify to the institution’s healthcare provider for appropriate counseling and guidance.
BMBL: B2 | Laboratory personnel must be provided medical surveillance and offered appropriate immunizations for agents handled or potentially present in the laboratory.
BMBL: B3 | When appropriate and giving consideration to the agent handled based upon the facility's risk assessment, each institution must establish policies and procedures describing the collection and storage of baseline serum samples from at-risk personnel. Additional serum specimens may be periodically collected, depending on the agents handled or the function of the laboratory.
NIH: G-II: B-2-m BMBL: B4 | A laboratory-specific biosafety manual is prepared or adopted, and must be available and accessible upon request.
NIH: G-II: B-2-d NIH: G-II: B-2-d BMBL: A9 | When organisms containing rDNA molecules or experimental animals are present in the laboratory or containment module, a hazard warning sign incorporating the universal biosafety symbol is posted on all laboratory and animal room access doors, in accordance with the institutional policy. The hazard warning sign identifies the agent(s), lists the name and telephone number of the Principal Investigator or other responsible person(s), and indicates any special requirements for entering the laboratory such as the need for immunizations, respirators, or other personal protective measures.
BMBL: B8 | Incidents that  result in potential exposure to infectious materials must be immediately evaluated and treated according to procedures described in the laboratory biosafety manual. All such incidents must be reported to the laboratory supervisor.
NIH: G-II: B-2-k | Spills and accidents which result in overt exposures to organisms containing recombinant DNA molecules are immediately reported to the Biological Safety Officer, Institutional Biosafety Committee, and NIH/OBA. Reports to NIH/OBA shall be sent to the Office of Biotechnology Activities, National Institutes of Health, 6705 Rockledge Drive, [NAME] 750, MSC 7985, Bethesda, MD 20892-7985 (20817 for non-USPS mail), [PHONE], [PHONE] (fax).
NIH: G-II: B-2-k BMBL: B8 | Post-exposure medical evaluation, surveillance, and treatment should be provided and appropriate records maintained.
BMBL: A5 | Policies for the safe handling of sharps, such as needles, scalpels, pipettes, and broken glassware must be developed and implemented.
BMBL: A5 BMBL: A5-d | Whenever practical, laboratory supervisors should adopt improved engineering and work practice controls that reduce risk of sharps injuries. Plasticware should be substituted for glassware whenever possible.
BMBL: A10 | An effective integrated pest management program is required.
BMBL: B9 NIH: G-II: B-2-g | Animals and plants not associated with the work being performed must not be permitted in the laboratory.
B | Laboratory Practices
NIH: G-II:B-1-e BMBL: A3 | Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing food for human consumption must not be permitted in laboratory areas. Food may be stored outside the laboratory in cabinets or refrigerators designated and used for this purpose only.
NIH: G-II:B-1-d BMBL: A4 | Mechanical pipetting devices are used; mouth pipetting is prohibited.
NIH: G-II:B-1-f BMBL: C4-c BMBL: A2 | Handwashing protocols must be rigorously followed. Persons wash their hands: (i) after handling materials involving potentially hazardous materials or organisms containing rDNA molecules, after handling animals, and (ii) when exiting the laboratory.
NIH: G-II:B-1-g BMBL: A6 | All procedures are performed carefully to minimize the creation of splashes or aerosols.
NIH: G-II: B-2-j BMBL: A5 BMBL: A5-a | Hypodermic needles and syringes are used only for parenteral injection and aspiration of fluids from laboratory animals and diaphragm bottles.  Only needlelocking syringes or disposable syringe-needle units (i.e., needle is integral to the syringe) are used for the injection or aspiration of fluids containing organisms that contain rDNA molecules. Extreme caution should be used when handling needles and syringes to avoid autoinoculation and the generation of aerosols during use and disposal. [NAME] should not be bent, sheared, replaced in the needle sheath or guard, removed from the syringe, or otherwise manipulated by hand following use.
NIH: G-II: B-2-j BMBL: A5-b | The needle and syringe should be promptly placed in a conveniently placed puncture-resistant container and decontaminated, preferably by autoclaving, before discard.
BMBL: A5-c | Non-disposable sharps must be placed in a hard walled container for transport to a processing area for decontamination, preferably by autoclaving.
BMBL: A5-d | [NAME] glassware must not be handled directly.  Instead, it must be removed using a brush and dustpan, tongs, or forceps.
BMBL: B6 | Potentially infectious materials must be placed in a durable, leak proof container during collection, handling, processing, storage, or transport within a facility.
NIH: G-II:B-1-b BMBL: A7 BMBL: B7 | The work surfaces of biological safety cabinets and other laboratory equipment are decontaminated routinely with appropriate disinfectant:  when work with organisms containing recombinant DNA molecules is finished, and after any spill or splash of potentially infectious material or other event leading to potential contamination.  Plastic-backed paper toweling used on non-perforated work surfaces within biological safety cabinets facilitates clean-up.
NIH: G-II:B-2-i NIH: G-II: B-2-a NIH: G-II: B-1-c BMBL: A8 BMBL: A8-a BMBL: A8-b | All wastes, cultures, stocks, and other potentially infectious materials  from laboratories and animal rooms are appropriately decontaminated before disposal. Contaminated materials that are to be decontaminated at a site away from the laboratory are placed in a durable leak-proof container which is closed before being removed from the laboratory, in accordance with applicable local, state, and federal regulations.
BMBL: B7-a | Spills involving infectious materials must be contained, decontaminated, and cleaned up by staff properly trained and equipped to work with infectious material.
BMBL: 7-b | Equipment must be decontaminated before repair, maintenance, or removal from the laboratory.
C | Personal Protective Equipment (PPE)
NIH: G-II: B-2-f BMBL: C2 | Laboratory coats, gowns, smocks, or uniforms are worn while in the laboratory, and are not worn outside the laboratory.  This protective clothing is removed and left in the laboratory or covered with a clean coat not used in the laboratory.
BMBL: C2 | Reusable clothing is changed when contaminated, and is decontaminated with appropriate disinfectant before being laundered.  It is recommended that laboratory clothing not be taken home.
NIH: G-II: B-2-h BMBL: C4 NIH: G-II: B-2-h | Special care is taken to avoid skin contamination with agents containing recombinant DNA molecules. Gloves, selected on the basis of an appropriate risk assessment, must be worn to protect hands from exposure to hazardous materials.
BMBL: C4 | Alternatives to latex gloves should be available.
BMBL: C4-a | BSL-2 laboratory workers should wear two pairs of gloves when appropriate.
BMBL: C4-a BMBL: C4-c | BSL-2 laboratory workers should change gloves when contaminated, integrity has been compromised, or when otherwise necessary. Disposable gloves should not be washed or re-used.
BMBL: C4-b BMBL: C4-c | BSL-2 laboratory workers should remove gloves, dispose of used gloves with other contaminated laboratory waste, and wash hands when work with hazardous materials has been completed and before leaving the laboratory. Gloves must not be worn outside the laboratory.
BMBL: C3 | Eye and face protection (goggles, mask, face shield or other splatter guard) is used for anticipated splashes or sprays of infectious or other hazardous materials when the biohazardous agents  must be handled outside the BSC or containment device.
BMBL: C3 | Persons who wear contact lenses in laboratories must also wear eye protection.
BMBL: C3 | Eye and face protection must be disposed of with other contaminated laboratory waste or decontaminated before reuse.
BMBL: C5 | Eye, face, and respiratory protection must be used in BSL-2 rooms containing infected animals as determined by the risk assessment.
D | [NAME]
BMBL: C1-a NIH: G-II: B-3-a-(1) BMBL: B10 NIH: G-II: C-3-a | Properly maintained BSCs (preferably Class II), other appropriate personal protective equipment, or other physical containment devices must be used whenever: Procedures with a potential for creating infectious aerosols or splashes are conducted.  These may include pipetting, centrifuging, grinding, blending, shaking, mixing, sonicating, opening containers of infectious materials.  Inoculating animals intranasally, and harvesting infected tissues from animals or eggs, and the necropsy of experimental animals.
BMBL: C1-b NIH: G-II: B-3-a-(2) | Properly maintained BSCs (preferably Class II), other appropriate personal protective equipment, or other physical containment devices must be used whenever: High concentrations or large volumes of infectious agents are used.  Such materials may be centrifuged in the open laboratory using sealed rotor heads or centrifuge safety cups,and if they are opened only in a biological safety cabinet.
BMBL: D6 | BSCs should be located away from doors, windows that can be opened, heavily traveled laboratory areas, and other possible airflow disruptions, and so that fluctuations of the room air supply and exhaust do not interfere with proper operations.
E | Housekeeping and General Lab Safety
BMBL: D3 NIH: G-II: B-4-a | The laboratory should be designed so that it can be easily cleaned and decontaminated.
BMBL: D3 | Carpets and rugs in laboratories are not permitted.
NIH: G-II: B-4-b BMBL: D4-a | Bench tops are impervious to water and resistant to acids, alkalis, organic solvents, and moderate heat.
NIH: G-II: B-4-c BMBL: D4 | Laboratory furniture must be capable of supporting anticipated loads and uses, and spaces between benches, cabinets, and equipment are accessible for cleaning.
BMBL: D4-b | Chairs used in laboratory work must be covered with a non-porous material that can be easily cleaned and decontaminated with appropriate disinfectant.
BMBL: D2 NIH: G-II: B-4-d | Laboratories must have a sink for hand washing.  The sink may be manually, hands free, or automatically operated.  It should be located near the exit door.
BMBL: D5 NIH: G-II: B-4-e | Laboratory windows that open to the exterior are not recommended.  However, if a laboratory does have windows that open to the exterior, they must be fitted with fly screens.
NIH: G-II: C-2-a BMBL: D1 | Laboratory doors are kept closed when experiments are in progress,must be self closing and have locks in accordance with the institutional policies.
BMBL: D11 NIH: G-II: B-4-f | A method for decontaminating all laboratory wastes should be available in the facility (e.g. autoclave, chemical disinfection, incineration, or other validated decontamination method).
BMBL: D8 | An eyewash station must be readily available.
BMBL: D9 | There are no specific requirements on ventilation systems.  However, planning of new facilities should consider mechanical ventilation systems that provide an inward flow of air without recirculation to spaces outside of the laboratory.
Miscellaneous
Inspector Summary and Comments
Inspector completing checklist |  | Date
Other Inspectors Present                                                                                                                      Date