Spreadsheet: 04e3ff8c-0042-40c1-a3f3-4d98dbfde824
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Additional file 5. Summary of promoter-fusion analyses. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12
 |  |  |  |  | FG (1 DAE) |  |  | Seed (stage 16) |  |  | Sporophytic tissue
AGI | Gene family 1 | Gene name 2 | Reporter |  | Pattern | T1 lines |  | Pattern | T1 lines |  | Pattern | T1 lines
AT1G06160 | AP2-EREBP | ORA59 | n1GFP |  | ne | 6 |  | ne | 5 |  | sp | 14
AT1G28160 | AP2-EREBP |  | n1GFP |  | ne | 5 |  | ne | 5 |  | sp | 7
AT1G35520 | ARF | ARF15 | n1GFP |  | A,s | 8 |  | ne | 7 |  | sp | 11
AT1G43160 | AP2-EREBP | RAP2.6 | n1GFP |  | ne | 5 |  | ne | 5 |  | SP | 6
[NAME] | bHLH | ZOU/RGE1/bHLH95 | cGFP |  | [NAME] | 7 |  | EN | 7 |  | sp | 7
AT1G55600 | WRKY | MINI3/WRKY10 | n1GFP |  | c | 7 |  | EN3 | 7 |  | sp | 7
AT1G60280 | NAC | NAC23 | n1GFP |  | [NAME]) | 7 |  | em,en | 7 |  | sp | 10
AT1G67030 | C2H2 | ZFP6 | n2GFP |  | A | 7 |  | ne | 7 |  | sp | 13
AT1G75250 | MYB-related | RL6 | cGFP |  | C,E,S | 13 |  | EN | 2 |  | nd
AT1G75490 | AP2-EREBP |  | n1GFP |  | ne | 6 |  | EN | 6 |  | sp | 8
AT2G22750 | bHLH |  | cGFP |  | (A)4 | 5 |  | ne | 5 |  | ne | 5
AT2G33710 | AP2-EREBP |  | n1GFP |  | E,(S) | 7 |  | EM,EN | 11 |  | sp | 23
AT2G40220 | AP2-EREBP | ABI4 | n1GFP |  | E | 9 |  | EM | 7 |  | sp | 10
AT3G01030 | C2H2 |  | n2GFP |  | S,(C),e | 8 |  | en5 | 11 |  | SP | 24
AT5G01380 | Trihelix |  | n1GFP |  | A,s | 7 |  | em | 7 |  | ne | 9
AT5G01860 | C2H2 |  | n2GFP |  | A,C,(E),(S) | 8 |  | (EN) | 9 |  | sp | 10
AT5G01860 |  |  | n1GFP |  | A,C,(E),(S) | 8 |  | (EN) | 11 |  | sp | 15
AT5G01860 |  |  | cGFP |  | a,C,(E),(S) | 6 |  | EN | 6 |  | sp | 7
AT5G11050 | MYB | MYB64 | cGFP |  | C,(E),(S) | 16 |  | EN | 2 |  | nd
AT5G15830 | bZIP | BZIP3 | n1GFP |  | ne | 5 |  | ne | 5 |  | SP | 19
AT5G27880 | C2H2 |  | n2GFP |  | C,a | 7 |  | EN | 7 |  | sp | 11
AT5G27880 |  |  | n1GFP |  | C,a | 7 |  | EN | 9 |  | sp | 15
AT5G27880 |  |  | cGFP |  | C | 5 |  | EN | 7 |  | ne | 7
AT5G41090 | NAC | NAC95 | n2GFP |  | A6 | 8 |  | ne | 7 |  | sp | 15
AT5G45980 | Homeobox | WOX8 | n1GFP |  | E,s | 8 |  | EM | 8 |  | sp | 11
AT5G50490 | CCAAT-HAP5 |  | n1GFP |  | C | 6 |  | EN | 12 |  | sp | 24
[NAME] | Homeobox | HB52 | n1GFP |  | ne | 6 |  | em,en5 | 6 |  | SP | 8
AT5G56200 | C2H2 |  | n1GFP |  | A,c,e,s | 5 |  | EN3 | 15 |  | sp | 24
GFP activity of 28 promoter fusions representing 24 transcription-factor genes were analyzed at one day after emasculation (1 DAE, corresponding to the mature stage of female gametophyte development), young seeds at flower stage 16 (corresponding to endosperm stages V to VI and embryo stages from elongated zygote to two-cell embryo-proper), and sporophytic tissues of the ovary. Description of each GFP expression pattern is accompanied by the number of T1 lines analyzed at the corresponding stage of development. Predominant, consistent patterns of GFP expression are noted in upper case, while the patterns observed in rare instances are in lower case. A, antipodal cells; C, central cell; E, egg cell; EM, embryo; EN, endosperm; ne, no expression; nd, not determined; S, synergid cells; SP, sporophytic tissues; (), weak expression.    1 Based on the classification of Arabidopsis transcription-factor genes provided by AGRIS http://arabidopsis.med.ohio-state.edu/AtTFDB/ and DATF http://datf.cbi.pku.edu.cn/ databases.   2 Based on gene annotation provided by TAIR http://www.arabidopsis.org/.   3 GFP activity was more intense in the micropylar endosperm compared to the chalazal and peripheral endosperm.   4 GFP activity was not detected at FG7 but was detected at FG5.   5 GFP activity was not detected in the endosperm after stage IV.   6 GFP activity was detected in both the nucleus and the cytoplasm of the antipodal cells.