Spreadsheet: 26759bed-c415-4bf8-865b-e62b3be9a9ab
Sheet: Sheet1

[Main strains library] | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6
ID | plt | rw | col | X1 | X2 | SGD description of X1
1 | 1 | A | 1 | empty | empty
2 | 1 | A | 2 | GDH3 | GDH1 | NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources
3 | 1 | A | 3 | GPB2 | GPB1 | Proposed beta subunit of the heterotrimeric G protein; interacts with the receptor Grp1p, affects signaling by altering the level of phosphorylation of PKS substrates; regulates pseudohyphal growth through cAMP levels; homolog of Gpb2p
4 | 1 | A | 4 | [NAME] | YOR342C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus
5 | 1 | A | 5 | [NAME] | SNC2 | Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins
6 | 1 | A | 6 | MYO4 | MYO2 | One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle
7 | 1 | A | 7 | FRT2 | FRT1 | Tail-anchored endoplasmic reticulum membrane protein that is a substrate of the phosphatase calcineurin, interacts with homolog Frt2p, promotes cell growth in conditions of high Na+, [NAME], and cell wall stress
8 | 1 | A | 8 | [NAME] | PMT3 | Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; target for new antifungals
9 | 1 | A | 9 | [NAME] | [NAME] | Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
10 | 1 | A | 10 | PSK1 | PSK2 | One of two (see also PSK1) PAS domain containing S/T protein kinases; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation)
11 | 1 | A | 11 | NTG1 | [NAME] | DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus
12 | 1 | A | 12 | empty | empty
13 | 1 | B | 1 | UIP3 | YAR028W | Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
14 | 1 | B | 2 | YAR028W | UIP3 | Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
15 | 1 | B | 3 | SWH1 | OSH2 | Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
16 | 1 | B | 4 | YEH1 | YEH2 | [NAME] ester hydrolase, [NAME] ester hydrolysis at the plasma membrane; involved in sterol metabolism
17 | 1 | B | 5 | [NAME] | SPH1 | Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p
18 | 1 | B | 6 | TPO1 | [NAME] | Polyamine transport protein, recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane; member of the major facilitator superfamily
19 | 1 | B | 7 | RPL8B | RPL8A | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits
20 | 1 | B | 8 | RNP1 | [NAME] | Nucleolar single-strand nucleic acid binding protein; associates with small nuclear RNAs
21 | 1 | B | 9 | YEH2 | YEH1 | [NAME] ester hydrolase, one of three gene products (Yeh1p, Yeh2p, [NAME]) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
22 | 1 | B | 10 | SNF7 | VPS20 | Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
23 | 1 | B | 11 | ADE16 | ADE17 | Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade16p; ade16 ade17 mutants require adenine and histidine
24 | 1 | B | 12 | RPS0B | RPS0A | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal
25 | 1 | C | 1 | empty | empty
26 | 1 | C | 2 | RPL22A | RPL22B | Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Ap and to rat L22 ribosomal protein
27 | 1 | C | 3 | EMP46 | EMP47 | Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
28 | 1 | C | 4 | ALT1 | ALT2 | Putative alanine transaminase (glutamic pyruvic transaminase)
29 | 1 | C | 5 | [NAME] | KIN1 | Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to [NAME]
30 | 1 | C | 6 | ICT1 | ECM18 | Protein of unknown function, similar to Rlp24p
31 | 1 | C | 7 | [NAME] | YDR132C | Putative protein of unknown function
32 | 1 | C | 8 | YML087C | PGA3 | Essential protein required for maturation of Gas1p and Pho8p, protein trafficking; GFP-fusion protein localizes to the endoplasmic reticulum; null mutants have a cell separation defect
33 | 1 | C | 9 | RPS1B | [NAME] | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein
34 | 1 | C | 10 | PIF1 | RRM3 | DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p
35 | 1 | C | 11 | CMP2 | [NAME] | Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
36 | 1 | C | 12 | [NAME] | [NAME] | Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
37 | 1 | D | 1 | SRC1 | HEH2 | Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
38 | 1 | D | 2 | TSA1 | TSA2 | Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants
39 | 1 | D | 3 | RPS18B | RPS18A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Bp and has similarity to E. coli S13 and rat S18 ribosomal proteins
40 | 1 | D | 4 | RPS17A | RPS17B | Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Ap and has similarity to rat S17 ribosomal protein
41 | 1 | D | 5 | PPZ1 | PPZ2 | Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
42 | 1 | D | 6 | RAD33 | YHR009C | Putative protein of unknown function; not an essential gene
43 | 1 | D | 7 | YAP1 | CAD1 | AP-1-like bZIP transcriptional activator involved in multiple stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins, binds consensus sequence TTACTAA; 5' UTR contains uORFs
44 | 1 | D | 8 | SOK2 | PHD1 | Transcriptional activator that enhances pseudohyphal growth; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to [NAME], an A. nidulans developmental regulator; potential Cdc28p substrate
45 | 1 | D | 9 | YET2 | [NAME] | Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein
46 | 1 | D | 10 | ARG80 | [NAME] | Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
47 | 1 | D | 11 | NUP53 | ASM4 | Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p
48 | 1 | D | 12 | YMR155W | MCH1 | Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
49 | 1 | E | 1 | [NAME] | ALD2 | Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p
50 | 1 | E | 2 | ALD2 | [NAME] | Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
51 | 1 | E | 3 | SSO2 | SSO1 | Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that [NAME]; syntaxin homolog; functionally redundant with Sso2p
52 | 1 | E | 4 | HSC82 | HSP82 | Cytoplasmic chaperone ([NAME] family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor [NAME] for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p
53 | 1 | E | 5 | GYL1 | GYP5 | GTPase-activating protein (GAP) for yeast Rab family members, involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by [NAME], also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p
54 | 1 | E | 6 | RPL36A | RPL36B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Ap and has similarity to rat [NAME] ribosomal protein; binds to 5.8 S rRNA
55 | 1 | E | 7 | [NAME] | ICY2 | Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate
56 | 1 | E | 8 | CLN1 | CLN2 | [NAME] involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, [NAME] (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
57 | 1 | E | 9 | HFA1 | ACC1 | Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA; required for de novo biosynthesis of long-chain fatty acids
58 | 1 | E | 10 | TRI1 | UAF30 | Subunit of UAF (upstream activation factor), which is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, [NAME], histones H3 and H4; deletion decreases cellular growth rate
59 | 1 | E | 11 | BCH1 | BUD7 | Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane
60 | 1 | E | 12 | RPL20A | RPL20B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Ap and has similarity to rat L18a ribosomal protein
61 | 1 | F | 1 | [NAME] | COT1 | Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium
62 | 1 | F | 2 | FAA4 | FAA1 | Long chain fatty acyl-CoA synthetase with a preference for C12:0-C16:0 fatty acids; involved in the activation of imported fatty acids; localized to both lipid particles and mitochondrial outer membrane; essential for stationary phase
63 | 1 | F | 3 | TPS3 | TSL1 | Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts [NAME] and glucose 6-phosphate to trehalose, homologous to Tps3p and may share function
64 | 1 | F | 4 | CUE1 | CUE4 | Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
65 | 1 | F | 5 | YMR265C | NAB6 | Putative RNA-binding protein, based on computational analysis of large-scale protein-protein interaction data
66 | 1 | F | 6 | TMA23 | PXR1 | Essential protein involved in rRNA and [NAME] maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
67 | 1 | F | 7 | ZDS1 | ZDS2 | Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; paralog of Zds1p
68 | 1 | F | 8 | BUL1 | BUL2 | Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1
69 | 1 | F | 9 | YMR310C | YGR283C | Protein of unknown function; may interact with ribosomes, based on co-purification experiments; deletion mutant is resistant to fluconazole; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
70 | 1 | F | 10 | MSG5 | SDP1 | Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates [NAME] kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock
71 | 1 | F | 11 | RPS19B | RPS19A | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Bp
72 | 1 | F | 12 | TRF5 | PAP2 | Catalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), a nuclear poly (A) polymerase complex involved in RNA quality control; catalyzes polyadenylation of unmodified tRNAs, and [NAME] and rRNA precursors; disputed role as a DNA polymerase
73 | 1 | G | 1 | [NAME] | SKM1 | Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; proposed to be a downstream effector of [NAME] during polarized growth
74 | 1 | G | 2 | [NAME] | [NAME] | GTPase-activating protein of the [NAME] superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; [NAME] double mutation causes defects in secretion and actin organization
75 | 1 | G | 3 | CAF120 | SKG3 | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p
76 | 1 | G | 4 | ALP1 | [NAME] | Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
77 | 1 | G | 5 | PDR17 | PDR16 | Phosphatidylinositol transfer protein (PITP) controlled by the multiple drug resistance regulator Pdr1p, localizes to lipid particles and microsomes, controls levels of various lipids, may regulate lipid synthesis, homologous to Pdr17p
78 | 1 | G | 6 | [NAME] | YSP1 | Mitochondrial protein with a potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone
79 | 1 | G | 7 | [NAME] | PRB1 | Vacuolar proteinase B (yscB), a serine protease of the [NAME] family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation
80 | 1 | G | 8 | [NAME] | AKR1 | Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats
81 | 1 | G | 9 | [NAME] | GLO2 | Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
82 | 1 | G | 10 | YOR062C | YKR075C | Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
83 | 1 | G | 11 | YOR066W | YKR077W | Putative transcriptional activator, identified by high-throughput two-hybrid experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YKR077W is not an essential gene
84 | 1 | G | 12 | VPS5 | YKR078W | Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P)
85 | 1 | H | 1 | empty | empty
86 | 1 | H | 2 | TGL5 | TGL4 | Triacylglycerol lipase involved in triacylglycerol mobilization and degradation; found in lipid particles; potential Cdc28p substrate
87 | 1 | H | 3 | TCB1 | TCB2 | Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud via the mRNA transport system involving She2p
88 | 1 | H | 4 | ECM3 | YNL095C | Putative protein of unknown function predicted to contain a transmembrane domain; [NAME] is not an essential gene
89 | 1 | H | 5 | UAF30 | TRI1 | Non-essential sumoylated protein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus
90 | 1 | H | 6 | BUD7 | BCH1 | Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p; may interact with ribosomes
91 | 1 | H | 7 | RPL20B | RPL20A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Bp and has similarity to rat L18a ribosomal protein
92 | 1 | H | 8 | COT1 | [NAME] | Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; also has a role in resistance to zinc shock resulting from a sudden influx of zinc into the cytoplasm
93 | 1 | H | 9 | PMT3 | [NAME] | Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt1p, can instead interact with Pmt5p in some conditions; target for new antifungals
94 | 1 | H | 10 | MRS2 | LPE10 | Mitochondrial inner membrane magnesium transporter, involved in maintenance of magnesium concentrations inside mitochondria; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
95 | 1 | H | 11 | PYK2 | CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration
96 | 1 | H | 12 | empty | empty
97 | 2 | A | 1 | empty | empty
98 | 2 | A | 2 | GPB1 | GPB2 | Proposed beta subunit of the heterotrimeric G protein; interacts with the receptor Grp1p, affects signaling by altering the level of phosphorylation of PKS substrates; regulates pseudohyphal growth through cAMP levels; homolog of Gpb1p
99 | 2 | A | 3 | ATF1 | ATF2 | Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important in brewing
100 | 2 | A | 4 | [NAME] | IOC3 | Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing
101 | 2 | A | 5 | [NAME] | [NAME] | Component of the [NAME]-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
102 | 2 | A | 6 | [NAME] | NTG1 | DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus and mitochondrion
103 | 2 | A | 7 | PSK2 | PSK1 | One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status
104 | 2 | A | 8 | GPM3 | GPM2 | [NAME] of Gpm1p phosphoglycerate mutase which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
105 | 2 | A | 9 | GPD2 | GPD1 | NAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p
106 | 2 | A | 10 | HST1 | SIR2 | Conserved NAD+ dependent histone deacetylase of the [NAME] family involved in regulation of lifespan;  plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication
107 | 2 | A | 11 | IRA2 | [NAME] | GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase
108 | 2 | A | 12 | empty | empty
109 | 2 | B | 1 | YOL083W | ATG19 | Protein involved in the cytoplasm-to-vacuole targeting pathway and in autophagy, recognizes cargo proteins and delivers them to the preautophagosomal structure for eventual engulfment by the autophagosome and degradation
110 | 2 | B | 2 | APM1 | APM2 | Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
111 | 2 | B | 3 | CLN2 | CLN1 | [NAME] involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, [NAME] (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
112 | 2 | B | 4 | VIK1 | CIK1 | Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p
113 | 2 | B | 5 | ICY2 | [NAME] | Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation
114 | 2 | B | 6 | GYP5 | GYL1 | Putative GTPase activating protein (GAP) that may have a role in polarized exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p
115 | 2 | B | 7 | HSP82 | HSC82 | Cytoplasmic chaperone of the [NAME] family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock
116 | 2 | B | 8 | SSO1 | SSO2 | Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
117 | 2 | B | 9 | FLC1 | FLC3 | [NAME] transporter, similar to Flc1p and Flc2p; localized to the ER
118 | 2 | B | 10 | RPL1A | RPL1B | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Ap and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal
119 | 2 | B | 11 | PCL8 | PCL10 | Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate
120 | 2 | B | 12 | YPL216W | ITC1 | Component of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1
121 | 2 | C | 1 | AFT2 | AFT1 | Transcription factor involved in iron utilization and homeostasis; binds the consensus site PyPuCACCCPu and activates the expression of target genes in response to changes in iron availability
122 | 2 | C | 2 | empty | empty
123 | 2 | C | 3 | YPL191C | YGL082W | Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene
124 | 2 | C | 4 | GUP2 | GUP1 | Plasma membrane protein with a possible role in proton symport of glycerol; member of the [NAME] family of putative membrane-bound O-acyltransferases
125 | 2 | C | 5 | SET6 | SET5 | Zinc-finger protein of unknown function, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
126 | 2 | C | 6 | PRM4 | YDL010W | Putative thiol oxidoreductase; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; non-essential gene
127 | 2 | C | 7 | YPL141C | KIN4 | Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck
128 | 2 | C | 8 | MKK2 | [NAME] | Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p
129 | 2 | C | 9 | UME1 | WTM2 | Transcriptional repressor involved in regulation of meiosis and silencing; contains WD repeats
130 | 2 | C | 10 | ISU1 | ISU2 | Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable
131 | 2 | C | 11 | RDS2 | YBR239C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR239C is not an essential gene
132 | 2 | C | 12 | YPL113C | YGL185C | Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
133 | 2 | D | 1 | SSE1 | SSE2 | Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; highly homologous to the heat shock protein Sse1p
134 | 2 | D | 2 | YPL105C | SMY2 | Protein of unknown function that interacts with Myo2p; has similarity to [NAME]
135 | 2 | D | 3 | EEB1 | EHT1 | Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; contains esterase activity; localizes to lipid particles and the mitochondrial outer membrane
136 | 2 | D | 4 | RPS6B | RPS6A | Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to rat S6 ribosomal protein
137 | 2 | D | 5 | SMP1 | RLM1 | MADS-box transcription factor, component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p
138 | 2 | D | 6 | [NAME] | YDC1 | Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity
139 | 2 | D | 7 | KTR4 | [NAME] | Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
140 | 2 | D | 8 | DER1 | DFM1 | ER localized derlin-like family member involved in ER stress and homeostasis; not involved in ERAD or substrate retrotranslocation; interacts with CDC48; contains four transmembrane domains and two SHP boxes
141 | 2 | D | 9 | [NAME] | KTR4 | Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
142 | 2 | D | 10 | ERV15 | ERV14 | Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon
143 | 2 | D | 11 | SDS24 | SDS23 | One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation
144 | 2 | D | 12 | PYC2 | [NAME] | Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc2p but differentially regulated; mutations in the human homolog are associated with lactic acidosis
145 | 2 | E | 1 | YBR238C | RMD9 | Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes
146 | 2 | E | 2 | YBR239C | RDS2 | Zinc cluster transcriptional activator involved in conferring resistance to ketoconazole
147 | 2 | E | 3 | YBR242W | [NAME] | Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p
148 | 2 | E | 4 | GPX2 | HYR1 | Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor
149 | 2 | E | 5 | REI1 | REH1 | Protein of unknown function, similar to Rei1p but not involved in bud growth; contains dispersed C2H2 zinc finger domains
150 | 2 | E | 6 | UBC5 | UBC4 | Ubiquitin-conjugating enzyme (E2), mediates degradation of short-lived and abnormal proteins; interacts with E3-CaM in ubiquitinating calmodulin; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response
151 | 2 | E | 7 | YDR066C | YER139C | Putative protein of unknown function; YER139C has been shown to be required for growth at high temperatures; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
152 | 2 | E | 8 | SED1 | SPI1 | GPI-anchored, serine/threonine rich cell wall protein of unknown function; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p
153 | 2 | E | 9 | DNF2 | DNF1 | Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
154 | 2 | E | 10 | GRX3 | GRX4 | Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage
155 | 2 | E | 11 | BMH2 | BMH1 | 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, [NAME]/MAPK signaling, and rapamycin-sensitive signaling
156 | 2 | E | 12 | YDR107C | EMP70 | Protein whose 24kDa cleavage product is found in endosome-enriched membrane fractions, predicted to be a transmembrane protein
157 | 2 | F | 1 | KIN1 | [NAME] | Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to [NAME]
158 | 2 | F | 2 | YDR132C | [NAME] | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; [NAME] is not an esssential gene
159 | 2 | F | 3 | SWI5 | ACE2 | Transcription factor that activates expression of early G1-specific genes, localizes to daughter cell nuclei after cytokinesis and delays [NAME] in daughters, localization is regulated by phosphorylation; potential Cdc28p substrate
160 | 2 | F | 4 | ATP22 | RIF2 | Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
161 | 2 | F | 5 | SBE2 | SBE22 | Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
162 | 2 | F | 6 | GGA1 | GGA2 | Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi
163 | 2 | F | 7 | RGA2 | [NAME] | GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth
164 | 2 | F | 8 | EFT2 | EFT1 | Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin
165 | 2 | F | 9 | SXM1 | NMD5 | Karyopherin, a carrier protein involved in nuclear import of proteins; importin beta homolog
166 | 2 | F | 10 | HPT1 | XPT1 | Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine
167 | 2 | F | 11 | SIZ1 | NFI1 | SUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; involved in maintenance of proper telomere length
168 | 2 | F | 12 | RPL12B | RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins
169 | 2 | G | 1 | CAD1 | YAP1 | Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; activated by [NAME] through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; mediates resistance to cadmium
170 | 2 | G | 2 | PPZ2 | PPZ1 | Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
171 | 2 | G | 3 | [NAME] | CYC1 | Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration
172 | 2 | G | 4 | YEF1 | UTR1 | ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p)
173 | 2 | G | 5 | YEL047C | OSM1 | Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity
174 | 2 | G | 6 | RPL12A | RPL12B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins
175 | 2 | G | 7 | AFG3 | YTA12 | Component, with [NAME], of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes
176 | 2 | G | 8 | ACA1 | [NAME] | Basic leucine zipper (bZIP) transcription factor of the ATF/[NAME] family, activates transcription of genes involved in utilization of non-optimal carbon sources; involved in telomere maintenance
177 | 2 | G | 9 | CAJ1 | [NAME] | Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ
178 | 2 | G | 10 | GIP2 | PIG2 | Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase
179 | 2 | G | 11 | HMF1 | MMF1 | Mitochondrial protein involved in maintenance of the mitochondrial genome
180 | 2 | G | 12 | PCL6 | PCL7 | Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated
181 | 2 | H | 1 | empty | empty
182 | 2 | H | 2 | HOR2 | RHR2 | Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and, along with the Hor2p/Gpp2p isoform, osmotic stress
183 | 2 | H | 3 | YER067W | YIL057C | Putative protein of unknown function; expression induced under carbon limitation and repressed under high glucose
184 | 2 | H | 4 | PTP3 | PTP2 | Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus
185 | 2 | H | 5 | SER3 | SER33 | 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser3p
186 | 2 | H | 6 | PPT1 | TAH1 | HSP90 cofactor; interacts with Hsp82p, Pih1p, [NAME] and Rvb2, contains a single TPR domain with at least two TPR motifs
187 | 2 | H | 7 | ASN2 | ASN1 | Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway
188 | 2 | H | 8 | [NAME] | NCE102 | Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs)
189 | 2 | H | 9 | LSB1 | PIN3 | Protein that induces appearance of [PIN+] prion when overproduced
190 | 2 | H | 10 | TPO2 | TPO3 | Polyamine transport protein specific for spermine; localizes to the plasma membrane; member of the major facilitator superfamily
191 | 2 | H | 11 | SKN1 | KRE6 | Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functionally redundant with Skn1p
192 | 2 | H | 12 | empty | empty
193 | 3 | A | 1 | empty | empty
194 | 3 | A | 2 | RPL24B | RPL24A | Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate
195 | 3 | A | 3 | ATF2 | ATF1 | Alcohol acetyltransferase with potential roles in lipid and sterol metabolism; responsible for the major part of volatile acetate ester production during fermentation
196 | 3 | A | 4 | UBR1 | UBR2 | Cytoplasmic ubiquitin-protein ligase (E3)
197 | 3 | A | 5 | TDH3 | TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall
198 | 3 | A | 6 | SNG1 | YJR015W | Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene
199 | 3 | A | 7 | RPS0A | RPS0B | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal
200 | 3 | A | 8 | RPL8A | RPL8B | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits
201 | 3 | A | 9 | APM2 | APM1 | Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting
202 | 3 | A | 10 | LAG1 | [NAME] | Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p
203 | 3 | A | 11 | DOG1 | DOG2 | 2-deoxyglucose-6-phosphate phosphatase, member of a family of low molecular weight phosphatases, similar to Dog1p, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed
204 | 3 | A | 12 | empty | empty
205 | 3 | B | 1 | AAP1 | APE2 | Zinc-dependent metallopeptidase yscII, may have a role in obtaining leucine from dipeptide substrates; sequence coordinates have changed since RT-PCR analysis showed that the adjacent ORF YKL158W comprises the 5' exon of APE2/YKL157W
206 | 3 | B | 2 | CPR2 | [NAME] | Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER
207 | 3 | B | 3 | GIC1 | GIC2 | Protein of unknown function involved in initiation of budding and cellular polarization, interacts with [NAME] via the Cdc42/Rac-interactive binding (CRIB) domain
208 | 3 | B | 4 | SSF1 | [NAME] | Protein required for ribosomal large subunit maturation, functionally redundant with Ssf1p; member of the [NAME] family
209 | 3 | B | 5 | [NAME] | YSP2 | Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification
210 | 3 | B | 6 | TRR2 | TRR1 | Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress
211 | 3 | B | 7 | GGA2 | GGA1 | Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi
212 | 3 | B | 8 | DMA1 | DMA2 | Protein involved in regulating spindle position and orientation, functionally redundant with Dma1p; homolog of S. pombe Dma1 and H. sapiens Chfr
213 | 3 | B | 9 | TOM71 | TOM70 | Component (70 kDa) of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
214 | 3 | B | 10 | EPT1 | CPT1 | Cholinephosphotransferase, required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription
215 | 3 | B | 11 | NSG1 | [NAME] | Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins
216 | 3 | B | 12 | YCK1 | YCK2 | Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with [NAME] in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of [NAME]
217 | 3 | C | 1 | YSP1 | [NAME] | Protein that activates transcription through interaction with DNA-bound Snf1p, C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate
218 | 3 | C | 2 | KEL1 | KEL2 | Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
219 | 3 | C | 3 | empty | empty
220 | 3 | C | 4 | PEX18 | PEX21 | [NAME] required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with [NAME]
221 | 3 | C | 5 | YAP1801 | YAP1802 | Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1801p, member of the [NAME] protein family
222 | 3 | C | 6 | SOL3 | SOL4 | 6-phosphogluconolactonase with similarity to Sol3p
223 | 3 | C | 7 | YHR177W | YEL007W | Putative protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1)
224 | 3 | C | 8 | GND1 | GND2 | 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone
225 | 3 | C | 9 | YHR198C | YHR199C | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
226 | 3 | C | 10 | YHR199C | YHR198C | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
227 | 3 | C | 11 | RPS4B | RPS4A | Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein
228 | 3 | C | 12 | GBP2 | HRB1 | Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Gbp2p and Npl3p
229 | 3 | D | 1 | KCC4 | [NAME] | Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p
230 | 3 | D | 2 | GFD2 | YDR514C | Putative protein of unknown function
231 | 3 | D | 3 | SRO9 | SLF1 | RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts
232 | 3 | D | 4 | GLK1 | EMI2 | Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor [NAME]; required for sporulation; expression is regulated by glucose-repression transcription factors Mig1/2p
233 | 3 | D | 5 | APA1 | [NAME] | Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase II (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa1p
234 | 3 | D | 6 | LRE1 | HLR1 | Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A
235 | 3 | D | 7 | CIT2 | CIT3 | Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
236 | 3 | D | 8 | MAK31 | YGL080W | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
237 | 3 | D | 9 | HSP30 | YRO2 | Putative protein of unknown function; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p
238 | 3 | D | 10 | ACE2 | SWI5 | Transcription factor that activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase
239 | 3 | D | 11 | YLR177W | PSP1 | Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition
240 | 3 | D | 12 | TFS1 | YLR179C | Protein of unknown function, transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus; [NAME] is not essential
241 | 3 | E | 1 | YLR179C | TFS1 | Carboxypeptidase Y inhibitor, function requires acetylation by the NatB N-terminal acetyltransferase; phosphatidylethanolamine-binding protein involved in protein kinase A signaling pathway
242 | 3 | E | 2 | SAM1 | [NAME] | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)
243 | 3 | E | 3 | SKG3 | CAF120 | Part of the evolutionarily-conserved [NAME]-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation
244 | 3 | E | 4 | CLB4 | CLB3 | B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation
245 | 3 | E | 5 | CRR1 | CRH1 | Cell wall protein that functions in the transfer of chitin to beta(1-6)glucan, putative chitin transglycosidase
246 | 3 | E | 6 | YLR225C | YDR222W | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
247 | 3 | E | 7 | [NAME] | LAG1 | Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p
248 | 3 | E | 8 | YKL027W | YHR003C | Protein of unknown function, localized to the mitochondrial outer membrane
249 | 3 | E | 9 | PTM1 | [NAME] | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles
250 | 3 | E | 10 | PHD1 | SOK2 | Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; negatively regulates pseudohyphal differentiation; homologous to several transcription factors
251 | 3 | E | 11 | YKL050C | YMR031C | Protein of unknown function with similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; YMR031C is not an essential gene
252 | 3 | E | 12 | MSN4 | MSN2 | Transcriptional activator related to Msn4p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression
253 | 3 | F | 1 | NUP100 | NUP116 | Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor [NAME] and with karyopherin Kap95p; homologous to Nup100p
254 | 3 | F | 2 | SMY1 | KIP3 | Kinesin-related motor protein involved in mitotic spindle positioning
255 | 3 | F | 3 | YKL091C | SEC14 | Phosphatidylinositol/phosphatidylcholine transfer protein involved in coordinate regulation of PtdIns and PtdCho metabolism, products of which are regulators in Golgi to plasma membrane transport; functionally homologous to mammalian PITPs
256 | 3 | F | 4 | CWP1 | CWP2 | Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
257 | 3 | F | 5 | [NAME] | [NAME] | Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; [NAME] is not an essential gene
258 | 3 | F | 6 | [NAME] | YPK2 | Protein kinase with similarityto serine/threonine protein kinase Ypk1p; functionally redundant with [NAME] at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK
259 | 3 | F | 7 | PGM1 | PGM2 | Phosphoglucomutase, catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase
260 | 3 | F | 8 | RMA1 | [NAME] | Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
261 | 3 | F | 9 | YKL133C | YMR115W | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
262 | 3 | F | 10 | APE2 | AAP1 | Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation
263 | 3 | F | 11 | YKL161C | SLT2 | Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progression through the cell cycle; regulated by the PKC1-mediated signaling pathway
264 | 3 | F | 12 | PIR3 | PIR1 | O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle
265 | 3 | G | 1 | YKL187C | YLR413W | Putative protein of unknown function; YLR413W is not an essential gene
266 | 3 | G | 2 | RPS25A | RPS25B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similarity to rat S25 ribosomal protein
267 | 3 | G | 3 | RPL26B | RPL26A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Bp and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA
268 | 3 | G | 4 | [NAME] | BUD8 | Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
269 | 3 | G | 5 | YGR043C | TAL1 | Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
270 | 3 | G | 6 | SCM4 | YLR356W | Putative protein of unknown function with similarity to SCM4; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR356W is not an essential gene
271 | 3 | G | 7 | YGR052W | CAK1 | Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
272 | 3 | G | 8 | RSC1 | RSC2 | Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance
273 | 3 | G | 9 | [NAME] | VID22 | Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to [NAME] (vacuole import and degradation) vesicles
274 | 3 | G | 10 | RPL11B | RPL11A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Bp; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11
275 | 3 | G | 11 | KTR1 | KRE2 | Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation
276 | 3 | G | 12 | RAS1 | RAS2 | GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian [NAME] proto-oncogenes
277 | 3 | H | 1 | empty | empty
278 | 3 | H | 2 | RGS2 | SST2 | GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
279 | 3 | H | 3 | INP53 | INP52 | Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in endocytosis; hyperosmotic stress causes translocation to actin patches
280 | 3 | H | 4 | [NAME] | RGA2 | GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth
281 | 3 | H | 5 | EFT1 | EFT2 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin
282 | 3 | H | 6 | BAG7 | SAC7 | GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
283 | 3 | H | 7 | NFI1 | SIZ1 | SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
284 | 3 | H | 8 | [NAME] | RPS28B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Ap and has similarity to rat S28 ribosomal protein
285 | 3 | H | 9 | [NAME] | LCB5 | Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules
286 | 3 | H | 10 | DCS2 | DCS1 | Non-essential hydrolase involved in mRNA decapping, may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p
287 | 3 | H | 11 | PTP2 | PTP3 | Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm
288 | 3 | H | 12 | empty | empty
289 | 4 | A | 1 | empty | empty
290 | 4 | A | 2 | ODC2 | ODC1 | Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation
291 | 4 | A | 3 | ISU2 | ISU1 | Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
292 | 4 | A | 4 | YOR227W | [NAME] | Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments
293 | 4 | A | 5 | WTM2 | UME1 | Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p
294 | 4 | A | 6 | [NAME] | MKK2 | Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p
295 | 4 | A | 7 | KIN4 | YPL141C | Putative protein kinase; similar to Kin4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL141C is not an essential gene
296 | 4 | A | 8 | HES1 | KES1 | Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex
297 | 4 | A | 9 | FSH3 | FSH2 | Serine hydrolase that localizes to the cytoplasm; sequence is similar to Fsh1p and Fsh3p
298 | 4 | A | 10 | SWE1 | CHK1 | Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
299 | 4 | A | 11 | HAL5 | KKQ8 | Putative serine/threonine protein kinase with unknown cellular role
300 | 4 | A | 12 | empty | empty
301 | 4 | B | 1 | FBP26 | YLR345W | Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the [NAME]-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
302 | 4 | B | 2 | SNA3 | PMP3 | Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
303 | 4 | B | 3 | TIF2 | TIF1 | Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G
304 | 4 | B | 4 | MRS3 | MRS4 | Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with [NAME]; functions under low-iron conditions; may transport other cations in addition to iron
305 | 4 | B | 5 | PEX30 | PEX31 | Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p
306 | 4 | B | 6 | TMA10 | STF2 | Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein
307 | 4 | B | 7 | NMA1 | NMA2 | Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway
308 | 4 | B | 8 | RPS25B | RPS25A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similarity to rat S25 ribosomal protein
309 | 4 | B | 9 | RPL26A | RPL26B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Ap and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA
310 | 4 | B | 10 | YLR345W | FBP26 | Fructose-2,6-bisphosphatase, required for glucose metabolism
311 | 4 | B | 11 | DIC1 | [NAME] | S-adenosylmethionine transporter of the mitochondrial inner membrane, member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth
312 | 4 | B | 12 | ORM2 | ORM1 | Evolutionarily conserved protein with similarity to Orm2p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum
313 | 4 | C | 1 | BUD8 | [NAME] | Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole
314 | 4 | C | 2 | TAL1 | YGR043C | Protein of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; null mutant non-quiescent cells exhibit reduced reproductive capacity
315 | 4 | C | 3 | YLR356W | SCM4 | Potential regulatory effector of CDC4 function, suppresses a temperature-sensitive allele of CDC4, tripartite protein structure in which a charged region separates two uncharged domains, not essential for mitosis or meiosis
316 | 4 | C | 4 | empty | empty
317 | 4 | C | 5 | VID22 | [NAME] | Putative protein of unknown function; deletion mutant has increased glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
318 | 4 | C | 6 | [NAME] | STP4 | Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p
319 | 4 | C | 7 | [NAME] | ZIP2 | Meiosis-specific protein involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis
320 | 4 | C | 8 | REH1 | REI1 | Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
321 | 4 | C | 9 | RPS29A | RPS29B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Ap and has similarity to rat [NAME] and E. coli S14 ribosomal proteins
322 | 4 | C | 10 | YLR413W | YKL187C | Putative protein of unknown function; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
323 | 4 | C | 11 | FAR10 | [NAME] | Cytoplasmic protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p, Far7p, [NAME], and [NAME] that is involved in pheromone-induced cell cycle arrest; also localized to the endoplasmic reticulum membrane
324 | 4 | C | 12 | [NAME] | [NAME] | Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase
325 | 4 | D | 1 | LCB5 | [NAME] | Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
326 | 4 | D | 2 | RPS28B | [NAME] | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Bp and has similarity to rat S28 ribosomal protein
327 | 4 | D | 3 | DCS1 | DCS2 | Non-essential, stress induced regulatory protein containing a HIT (histidine triad) motif; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by [NAME], Msn4p, and the [NAME]-cAMP-cAPK signaling pathway, similar to Dcs1p.
328 | 4 | D | 4 | CTH1 | TIS11 | mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis
329 | 4 | D | 5 | YDR222W | YLR225C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene
330 | 4 | D | 6 | MDS3 | PMD1 | Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions
331 | 4 | D | 7 | SIP2 | GAL83 | One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain
332 | 4 | D | 8 | YPT32 | YPT31 | GTPase of the [NAME]/Rab family, very similar to Ypt32p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi
333 | 4 | D | 9 | KIP3 | SMY1 | Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins
334 | 4 | D | 10 | SDT1 | PHM8 | Protein of unknown function, expression is induced by low phosphate levels and by inactivation of Pho85p
335 | 4 | D | 11 | SHE10 | YFR039C | Putative protein of unknown function; YFR039C is not an essential gene
336 | 4 | D | 12 | SAP4 | SAP155 | Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p
337 | 4 | E | 1 | ZIP2 | [NAME] | Protein of unknown function; deletion results in a mutator phenotype suggesting a role for this protein as a mutational suppressor; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
338 | 4 | E | 2 | HXK2 | HXK1 | Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p
339 | 4 | E | 3 | PEX31 | PEX30 | Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
340 | 4 | E | 4 | STF2 | TMA10 | Protein of unknown function that associates with ribosomes
341 | 4 | E | 5 | NMA2 | NMA1 | Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway
342 | 4 | E | 6 | RPS6A | RPS6B | Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein
343 | 4 | E | 7 | RPS9A | [NAME] | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli [NAME] and rat S9 ribosomal proteins
344 | 4 | E | 8 | RPL21B | RPL21A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat [NAME] ribosomal protein
345 | 4 | E | 9 | YPL077C | YBR197C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene
346 | 4 | E | 10 | LPE10 | MRS2 | Mitochondrial inner membrane [NAME], required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns
347 | 4 | E | 11 | SUR1 | CSH1 | Probable catalytic subunit of a mannosylinositol phosphorylceramide ([NAME]) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p
348 | 4 | E | 12 | DIG1 | DIG2 | Regulatory protein of unknown function, pheromone-inducible, involved in the regulation of mating-specific genes and the invasive growth pathway, required for MAP-kinase imposed repression, inhibits pheromone-responsive transcription
349 | 4 | F | 1 | EGD1 | BTT1 | Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex (alpha, [NAME], beta3) which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides
350 | 4 | F | 2 | SVL3 | [NAME] | Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype
351 | 4 | F | 3 | SKS1 | VHS1 | Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p
352 | 4 | F | 4 | VTC3 | VTC2 | Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in protein localization and non-autophagic vacuolar fusion
353 | 4 | F | 5 | CIT3 | CIT2 | Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors
354 | 4 | F | 6 | [NAME] | DBF2 | Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis
355 | 4 | F | 7 | ASN1 | ASN2 | Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway
356 | 4 | F | 8 | PIN3 | LSB1 | Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human [NAME] Syndrome protein involved in actin patch assembly and actin polymerization
357 | 4 | F | 9 | TPO3 | TPO2 | Polyamine transport protein specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; member of the major facilitator superfamily
358 | 4 | F | 10 | YPR158W | [NAME] | v-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase
359 | 4 | F | 11 | YPR174C | [NAME] | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex
360 | 4 | F | 12 | HPA2 | HPA3 | D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro
361 | 4 | G | 1 | KIN82 | YNR047W | Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate
362 | 4 | G | 2 | CDC50 | YNR048W | Protein proposed to interact with phospholipid translocases, shares similarity to Cdc50p
363 | 4 | G | 3 | STF1 | INH1 | Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase, inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has similarity to Stf1p and both Inh1p and Stf1p exhibit the potential to form coiled-coil structures
364 | 4 | G | 4 | UBP5 | DOA4 | Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
365 | 4 | G | 5 | RPL22B | RPL22A | Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 ribosomal protein
366 | 4 | G | 6 | SAP155 | SAP4 | Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p
367 | 4 | G | 7 | CNN1 | RSC58 | Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance58KDa subunit
368 | 4 | G | 8 | HXK1 | HXK2 | Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and [NAME] and to induce expression of its own gene
369 | 4 | G | 9 | TOS2 | SKG6 | Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
370 | 4 | G | 10 | BNS1 | SPO12 | Nucleolar protein of unknown function, positive regulator of exit from mitosis; involved in regulating the release of Cdc14p from the nucleolus in early anaphase; proposed to play similar role in meiosis
371 | 4 | G | 11 | YAP1802 | YAP1801 | Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1802p, member of the [NAME] protein family
372 | 4 | G | 12 | YGR243W | YHR162W | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
373 | 4 | H | 1 | empty | empty
374 | 4 | H | 2 | PDR3 | PDR1 | Zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes
375 | 4 | H | 3 | ALK2 | ALK1 | Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk2p and to mammalian haspins
376 | 4 | H | 4 | URA7 | URA8 | Minor CTP synthase isozyme (see also URA7), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis
377 | 4 | H | 5 | SAS3 | ESA1 | Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression
378 | 4 | H | 6 | YBL054W | DOT6 | Protein of unknown function, involved in telomeric gene silencing and filamentation
379 | 4 | H | 7 | PTC3 | PTC2 | Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation
380 | 4 | H | 8 | UBP13 | UBP9 | Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions
381 | 4 | H | 9 | PRS4 | PRS2 | 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
382 | 4 | H | 10 | RPS8A | RPS8B | Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein
383 | 4 | H | 11 | SSA3 | SSA4 | Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation
384 | 4 | H | 12 | empty | empty
385 | 5 | A | 1 | empty | empty
386 | 5 | A | 2 | NUP170 | [NAME] | Abundant subunit of the nuclear pore complex (NPC), present on both sides of the NPC, has similarity to Nup170p
387 | 5 | A | 3 | BOI1 | [NAME] | Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain
388 | 5 | A | 4 | RPL23A | RPL23B | Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E. coli [NAME] and rat [NAME] ribosomal proteins
389 | 5 | A | 5 | ERP6 | ERP1 | Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles
390 | 5 | A | 6 | PDR1 | PDR3 | Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements)
391 | 5 | A | 7 | RPL24A | RPL24B | Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate
392 | 5 | A | 8 | TIF4632 | [NAME] | Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains [NAME] ([NAME]); associates with the poly(A)-binding protein [NAME], also interacts with eIF4A (Tif1p); homologous to [NAME]
393 | 5 | A | 9 | SDS23 | SDS24 | One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis
394 | 5 | A | 10 | [NAME] | PYC2 | Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis
395 | 5 | A | 11 | AFT1 | AFT2 | Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; similar to Aft1p
396 | 5 | A | 12 | empty | empty
397 | 5 | B | 1 | YGL081W | LDB19 | Protein of unknown function involved in maintenance of proper telomere length; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface
398 | 5 | B | 2 | YGL082W | YPL191C | Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
399 | 5 | B | 3 | [NAME] | CUP9 | Homeodomain-containing transcriptional repressor of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription
400 | 5 | B | 4 | PDR16 | PDR17 | Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition
401 | 5 | B | 5 | WHI3 | WHI4 | Putative RNA binding protein and partially redundant [NAME] homolog that regulates the cell size requirement for passage through Start and commitment to cell division
402 | 5 | B | 6 | [NAME] | FMP45 | Integral membrane protein localized to mitochondria (untagged protein) and eisosomes, immobile patches at the cortex associated with endocytosis; sporulation and sphingolipid content are altered in mutants; has homologs [NAME] and [NAME]
403 | 5 | B | 7 | [NAME] | YDL211C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
404 | 5 | B | 8 | MDG1 | CRP1 | Protein that binds to cruciform DNA structures
405 | 5 | B | 9 | YNL157W | YHR132W-A | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
406 | 5 | B | 10 | [NAME] | NSG1 | Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins
407 | 5 | B | 11 | YCK2 | YCK1 | Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p
408 | 5 | B | 12 | [NAME] | PTK2 | Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
409 | 5 | C | 1 | TRP3 | [NAME] | Para-aminobenzoate (PABA) synthase, has similarity to Escherichia coli PABA synthase components PabA and PabB
410 | 5 | C | 2 | MCH2 | ESBP6 | Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
411 | 5 | C | 3 | YKR018C | IML2 | Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
412 | 5 | C | 4 | ALY1 | ALY2 | Cytoplasmic protein of unknown function that interacts with the cyclin Pcl7p; phosphorylated in vitro by the cyclin-CDK complex, Pcl7p-Pho85p; identified as a potential Cdc28p substrate; mRNA is cell cycle regulated, peaking in M phase
413 | 5 | C | 5 | empty | empty
414 | 5 | C | 6 | MRS4 | MRS3 | Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrs4p; functions under low-iron conditions; may transport other cations in addition to iron
415 | 5 | C | 7 | GLG1 | GLG2 | Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin
416 | 5 | C | 8 | TIF1 | TIF2 | Translation initiation factor eIF4A, identical to Tif1p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G
417 | 5 | C | 9 | UTP30 | CIC1 | Essential protein that interacts with proteasome components and has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles
418 | 5 | C | 10 | VHS1 | SKS1 | Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the [NAME] regulated pathway
419 | 5 | C | 11 | [NAME] | SVL3 | Protein of unknown function, mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck
420 | 5 | C | 12 | BTT1 | EGD1 | [NAME] of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b
421 | 5 | D | 1 | YAP6 | CIN5 | Basic leucine zipper transcriptional factor of the yAP-1 family that mediates pleiotropic drug resistance and salt tolerance; localizes constitutively to the nucleus
422 | 5 | D | 2 | DIN7 | [NAME] | 5'-3' exonuclease and flap-endonuclease involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains
423 | 5 | D | 3 | AKR1 | [NAME] | Ankyrin repeat-containing protein similar to Akr1p; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p
424 | 5 | D | 4 | GLO2 | [NAME] | Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
425 | 5 | D | 5 | DON1 | [NAME] | Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
426 | 5 | D | 6 | PMP3 | SNA3 | Integral membrane protein localized to vacuolar intralumenal vesicles, computational analysis of large-scale protein-protein interaction data suggests a possible role in either cell wall synthesis or protein-vacuolar targeting
427 | 5 | D | 7 | [NAME] | CPR2 | Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
428 | 5 | D | 8 | GIC2 | GIC1 | Protein of unknown function involved in initiation of budding and cellular polarization, interacts with [NAME] via the Cdc42/Rac-interactive binding (CRIB) domain
429 | 5 | D | 9 | [NAME] | SSF1 | Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the [NAME] family
430 | 5 | D | 10 | RAD34 | [NAME] | Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 ([NAME]); homolog of human XPC protein
431 | 5 | D | 11 | [NAME] | SCS3 | Protein required for inositol prototrophy, appears to be involved in the synthesis of inositol phospholipids from inositol but not in the control of inositol synthesis
432 | 5 | D | 12 | YIL029C | YPR071W | Putative membrane protein; YPR071W is not an essential gene
433 | 5 | E | 1 | PIG2 | GIP2 | Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p
434 | 5 | E | 2 | PCL7 | PCL6 | Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by [NAME] binding
435 | 5 | E | 3 | RHR2 | HOR2 | One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition
436 | 5 | E | 4 | YIL057C | YER067W | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YER067W is not an essential gene
437 | 5 | E | 5 | PRK1 | ARK1 | Serine/threonine protein kinase involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis
438 | 5 | E | 6 | [NAME] | YEA6 | Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also [NAME]); has putative human ortholog
439 | 5 | E | 7 | [NAME] | NOT5 | Subunit of the [NAME]-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
440 | 5 | E | 8 | SER33 | SER3 | 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser33p
441 | 5 | E | 9 | HXT10 | HXT2 | High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose
442 | 5 | E | 10 | EMP47 | EMP46 | Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
443 | 5 | E | 11 | CMK1 | [NAME] | Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk1p and mammalian [NAME]
444 | 5 | E | 12 | [NAME] | [NAME] | Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase
445 | 5 | F | 1 | PES4 | MIP6 | Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export
446 | 5 | F | 2 | GND2 | GND1 | 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress
447 | 5 | F | 3 | YGR283C | YMR310C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene
448 | 5 | F | 4 | MGA2 | SPT23 | ER membrane protein involved in regulation of [NAME] transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
449 | 5 | F | 5 | YIR035C | YIR036C | Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52 foci
450 | 5 | F | 6 | YIR036C | YIR035C | Putative cytoplasmic protein of unknown function
451 | 5 | F | 7 | HYR1 | GPX2 | Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress
452 | 5 | F | 8 | YKR075C | YOR062C | Protein of unknown function; similar to [NAME] and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
453 | 5 | F | 9 | YKR078W | VPS5 | Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p
454 | 5 | F | 10 | VPS20 | SNF7 | One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
455 | 5 | F | 11 | YTA12 | AFG3 | Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes
456 | 5 | F | 12 | [NAME] | TBS1 | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
457 | 5 | G | 1 | ITR2 | ITR1 | Myo-inositol transporter with strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p
458 | 5 | G | 2 | [NAME] | [NAME] | GTPase-activating protein for [NAME] and several other Rab GTPases, regulates exocytosis via its action on Sec4p, also required for proper actin organization; similar to Msb4p; both Msb3p and Msb4p localize to sites of polarized growth
459 | 5 | G | 3 | SKM1 | [NAME] | Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p
460 | 5 | G | 4 | PAP2 | TRF5 | Poly (A) polymerase involved in nuclear RNA quality control based on: homology with Trf4p, genetic interactions with TRF4 mutants, physical interaction with Mtr4p (TRAMP subunit), and by direct assay; disputed role as a sigma DNA polymerase
461 | 5 | G | 5 | MSN1 | [NAME] | Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
462 | 5 | G | 6 | RPS19A | RPS19B | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Ap
463 | 5 | G | 7 | YER064C | VHR1 | Transcriptional activator, required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and [NAME] (biotin biosynthesis intermediate transporter) in response to low biotin concentrations
464 | 5 | G | 8 | DOT6 | YBL054W | Protein of unknown function involved in rRNA and ribosome biosynthesis
465 | 5 | G | 9 | YER093C-A | YBL059W | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
466 | 5 | G | 10 | SHC1 | SKT5 | Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation
467 | 5 | G | 11 | UBP9 | UBP13 | Putative ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions
468 | 5 | G | 12 | YSC84 | [NAME] | Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human [NAME] Syndrome protein involved in actin patch assembly and actin polymerization
469 | 5 | H | 1 | empty | empty
470 | 5 | H | 2 | YHR162W | YGR243W | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
471 | 5 | H | 3 | YLR031W | YMR124W | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene
472 | 5 | H | 4 | YMR181C | YPL229W | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL229W is not an essential gene
473 | 5 | H | 5 | [NAME] | URA5 | One of two orotate phosphoribosyltransferase isozymes (see also URA10) that catalyze the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate
474 | 5 | H | 6 | YMR279C | ATR1 | Multidrug efflux pump of the major facilitator superfamily, required for resistance to aminotriazole and 4-nitroquinoline-N-oxide
475 | 5 | H | 7 | [NAME] | CMK1 | Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk2p and mammalian [NAME]
476 | 5 | H | 8 | FAA1 | FAA4 | Long chain fatty acyl-CoA synthetase, regulates protein modification during growth in the presence of ethanol, functions to incorporate palmitic acid into phospholipids and neutral lipids
477 | 5 | H | 9 | FPR3 | FPR4 | Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
478 | 5 | H | 10 | TSL1 | TPS3 | Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the [NAME]-cAMP pathway
479 | 5 | H | 11 | CUE4 | CUE1 | Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination
480 | 5 | H | 12 | empty | empty
481 | 6 | A | 1 | empty | empty
482 | 6 | A | 2 | URA5 | [NAME] | One of two orotate phosphoribosyltransferase isozymes (see also URA5) that catalyze the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate
483 | 6 | A | 3 | ZDS2 | ZDS1 | Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; has a role in localization of Bcy1p, a regulatory subunit of protein kinase A; implicated in mRNA nuclear export
484 | 6 | A | 4 | NGL3 | [NAME] | Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p and Ngl3p
485 | 6 | A | 5 | TUB3 | TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules
486 | 6 | A | 6 | PGM2 | PGM1 | Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism
487 | 6 | A | 7 | ADE17 | ADE16 | Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine
488 | 6 | A | 8 | [NAME] | SUT1 | Transcription factor of the Zn[II]2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression
489 | 6 | A | 9 | CSR2 | ECM21 | Non-essential protein of unknown function; promoter contains several Gcn4p binding elements
490 | 6 | A | 10 | PUF2 | JSN1 | Member of the [NAME] family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells
491 | 6 | A | 11 | TKL1 | TKL2 | Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids
492 | 6 | A | 12 | empty | empty
493 | 6 | B | 1 | [NAME] | YKR011C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
494 | 6 | B | 2 | NCA3 | UTH1 | Mitochondrial outer membrane and cell wall localized SUN family member required for mitochondrial autophagy; involved in the oxidative stress response, life span during starvation, mitochondrial biogenesis, and cell death
495 | 6 | B | 3 | MDV1 | [NAME] | WD40 repeat-containing protein associated with the [NAME]-NOT complex, interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission
496 | 6 | B | 4 | CHS6 | BCH2 | Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane
497 | 6 | B | 5 | ALY2 | ALY1 | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
498 | 6 | B | 6 | IML2 | YKR018C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
499 | 6 | B | 7 | DLS1 | DPB3 | Third-largest subunit of DNA polymerase II (DNA polymerase epsilon), required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; mRNA abundance peaks at the G1/S boundary of the cell cycle
500 | 6 | B | 8 | UBX6 | UBX7 | UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p
501 | 6 | B | 9 | YPT31 | YPT32 | GTPase of the [NAME]/Rab family, very similar to [NAME]; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi
502 | 6 | B | 10 | BUB1 | MAD3 | Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover
503 | 6 | B | 11 | YHR003C | YKL027W | Protein of unknown function, localized to the mitochondrial outer membrane
504 | 6 | B | 12 | STP2 | STP1 | Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing
505 | 6 | C | 1 | YHR009C | RAD33 | Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
506 | 6 | C | 2 | URA8 | URA7 | Major [NAME] synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis
507 | 6 | C | 3 | TGL4 | TGL5 | Triacylglycerol lipase involved in TAG mobilization; localizes to lipid particles; potential Cdc28p substrate
508 | 6 | C | 4 | SRL3 | WHI5 | Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
509 | 6 | C | 5 | UBP11 | UBP7 | Ubiquitin-specific protease that cleaves ubiquitin-protein fusions
510 | 6 | C | 6 | empty | empty
511 | 6 | C | 7 | [NAME] | [NAME] | Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
512 | 6 | C | 8 | [NAME] | CMP2 | Calcineurin A; one isoform (the other is [NAME]) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
513 | 6 | C | 9 | YLR437C | SML1 | Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase
514 | 6 | C | 10 | [NAME] | RPS1B | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein
515 | 6 | C | 11 | FPR4 | FPR3 | Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p
516 | 6 | C | 12 | HMG2 | [NAME] | One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; localizes to the nuclear envelope; overproduction induces the formation of karmellae
517 | 6 | D | 1 | SST2 | RGS2 | Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
518 | 6 | D | 2 | RIF2 | ATP22 | Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
519 | 6 | D | 3 | YLR456W | YPR172W | Protein of unknown function, transcriptionally activated by Yrm1p along with genes involved in multidrug resistance
520 | 6 | D | 4 | JSN1 | PUF2 | Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer [NAME] binding activity; preferentially binds mRNAs encoding membrane-associated proteins
521 | 6 | D | 5 | XPT1 | HPT1 | Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the formation of both inosine monophosphate and [NAME]; mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
522 | 6 | D | 6 | RPS4A | RPS4B | Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to rat S4 ribosomal protein
523 | 6 | D | 7 | [NAME] | MSN1 | Transcriptional activator involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus
524 | 6 | D | 8 | ARF1 | ARF2 | ADP-ribosylation factor, GTPase of the [NAME] superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf1p
525 | 6 | D | 9 | YDL211C | [NAME] | Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p
526 | 6 | D | 10 | YDL218W | YNR061C | Putative protein of unknown function
527 | 6 | D | 11 | FMP45 | [NAME] | Integral membrane protein localized to eisosomes; sporulation and plasma membrane sphingolipid content are altered in mutants; has homologs [NAME] and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
528 | 6 | D | 12 | WHI4 | WHI3 | RNA binding protein that sequesters [NAME] in cytoplasmic foci; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start
529 | 6 | E | 1 | SSB1 | SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partner [NAME]; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; homolog of SSB1
530 | 6 | E | 2 | ADY3 | [NAME] | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration
531 | 6 | E | 3 | [NAME] | NTH2 | Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses
532 | 6 | E | 4 | [NAME] | [NAME] | Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane
533 | 6 | E | 5 | [NAME] | CST26 | Protein of unknown function, affects chromosome stability when overexpressed
534 | 6 | E | 6 | RPS11A | RPS11B | Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins
535 | 6 | E | 7 | YDR026C | [NAME] | [NAME] polymerase I enhancer binding protein; DNA binding protein which binds to genes transcribed by both [NAME] polymerase I and RNA polymerase II; required for termination of [NAME] polymerase I transcription
536 | 6 | E | 8 | UBX7 | UBX6 | UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline
537 | 6 | E | 9 | DPB3 | DLS1 | Subunit of ISW2/yCHRAC chromatin accessibility complex along with Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing
538 | 6 | E | 10 | YBR284W | [NAME] | Putative protein of unknown function with similarity to AMP deaminases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; [NAME] is a non-essential gene
539 | 6 | E | 11 | RPS14A | RPS14B | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins
540 | 6 | E | 12 | SOL2 | SOL1 | Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol2p, Sol3p, and Sol4p
541 | 6 | F | 1 | TDH2 | TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall
542 | 6 | F | 2 | [NAME] | GEA2 | Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p
543 | 6 | F | 3 | CYC1 | [NAME] | Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration
544 | 6 | F | 4 | OSM1 | YEL047C | Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies
545 | 6 | F | 5 | LYS21 | LYS20 | Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys21p
546 | 6 | F | 6 | PPH21 | PPH22 | Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis
547 | 6 | F | 7 | ARF2 | ARF1 | ADP-ribosylation factor, GTPase of the [NAME] superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p
548 | 6 | F | 8 | CLB3 | CLB4 | B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation
549 | 6 | F | 9 | INH1 | STF1 | Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein
550 | 6 | F | 10 | LYS20 | LYS21 | Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys20p
551 | 6 | F | 11 | PPH22 | PPH21 | Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis
552 | 6 | F | 12 | HRB1 | GBP2 | Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to [NAME] and Npl3p; also binds single-stranded telomeric repeat sequence in vitro
553 | 6 | G | 1 | ASI3 | ASI1 | Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and [NAME] to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
554 | 6 | G | 2 | ARK1 | PRK1 | Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton through the phosphorylation of the Pan1p-Sla1p-End3p protein complex
555 | 6 | G | 3 | SFB2 | SEC24 | Component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat; involved in ER to Golgi transport, cargo selection and autophagy; required for the binding of the Sec13 complex to ER membranes; homologous to Lst1p and Lss1p
556 | 6 | G | 4 | ARE2 | ARE1 | Acyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen
557 | 6 | G | 5 | BUD17 | BUD16 | Protein involved in bud-site selection and telomere maintenance; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; has similarity to pyridoxal kinases
558 | 6 | G | 6 | SOL1 | SOL2 | Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol1p, Sol3p, and Sol4p
559 | 6 | G | 7 | YNR048W | CDC50 | Endosomal protein that regulates cell polarity, controls polarized growth; similar to Ynr048wp and Lem3p
560 | 6 | G | 8 | SRO77 | SRO7 | Putative effector of Rab GTPase Sec4p, forms a complex with [NAME] and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro77p and Drosophila lgl tumor suppressor
561 | 6 | G | 9 | NTH2 | [NAME] | Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p
562 | 6 | G | 10 | [NAME] | [NAME] | Vacuolar protein that presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; similar to Rcr1p
563 | 6 | G | 11 | YBR016W | YDL012C | Plasma membrane protein of unknown function; YDL012C is not an essential gene
564 | 6 | G | 12 | GAL1 | GAL3 | Transcriptional regulator involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity
565 | 6 | H | 1 | empty | empty
566 | 6 | H | 2 | SCO2 | SCO1 | Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; has similarity to thioredoxins
567 | 6 | H | 3 | RPL4A | RPL4B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarity to E. coli L4 and rat [NAME] ribosomal proteins
568 | 6 | H | 4 | RPS11B | RPS11A | Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins
569 | 6 | H | 5 | YRO2 | HSP30 | Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
570 | 6 | H | 6 | MFA2 | MFA1 | Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2
571 | 6 | H | 7 | YNL144C | YHR131C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
572 | 6 | H | 8 | DMA2 | DMA1 | Protein involved in regulating spindle position and orientation, functionally redundant with Dma2p; homolog of S. pombe Dma1 and H. sapiens Chfr
573 | 6 | H | 9 | YNL108C | TFC7 | One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes
574 | 6 | H | 10 | AVT4 | [NAME] | Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
575 | 6 | H | 11 | RAS2 | RAS1 | GTPase involved in G-protein signaling in the adenylate cyclase activating pathway, plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes
576 | 6 | H | 12 | empty | empty
577 | 7 | A | 1 | empty | empty
578 | 7 | A | 2 | RPL16B | RPL16A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Bp, E. coli [NAME] and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p
579 | 7 | A | 3 | SDP1 | MSG5 | Dual-specificity protein phosphatase required for maintenance of a low level of signaling through the cell integrity pathway; regulates and is regulated by Slt2p; also required for adaptive response to pheromone
580 | 7 | A | 4 | POR2 | POR1 | Mitochondrial porin (voltage-dependent anion channel), outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated
581 | 7 | A | 5 | QDR1 | QDR2 | Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, barban, cisplatin, and bleomycin; may have a role in potassium uptake
582 | 7 | A | 6 | SIM1 | SUN4 | Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family
583 | 7 | A | 7 | VHS2 | MLF3 | Serine-rich protein of unknown function; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide
584 | 7 | A | 8 | MLP2 | MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with [NAME] in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs
585 | 7 | A | 9 | IOC3 | [NAME] | Protein involved in telomeric and mating-type locus silencing, interacts with Sir2p and also interacts with the Gal11p, which is a component of the RNA pol II mediator complex
586 | 7 | A | 10 | OCA2 | OCA1 | Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
587 | 7 | A | 11 | SUN4 | SIM1 | Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
588 | 7 | A | 12 | empty | empty
589 | 7 | B | 1 | RPL9B | RPL9A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Bp and has similarity to E. coli L6 and rat L9 ribosomal proteins
590 | 7 | B | 2 | [NAME] | FKH1 | Forkhead family transcription factor with a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; regulates donor preference during switching
591 | 7 | B | 3 | MLF3 | VHS2 | Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
592 | 7 | B | 4 | TPM1 | TPM2 | Minor isoform of tropomyosin, binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p
593 | 7 | B | 5 | TCB2 | TCB1 | Lipid-binding protein containing three calcium and lipid binding domains; non-tagged protein localizes to mitochondria and GFP-fusion protein localizes to the cell periphery; C-termini of Tcb1p, Tcb2p and Tcb3p interact
594 | 7 | B | 6 | YNL095C | ECM3 | Non-essential protein of unknown function
595 | 7 | B | 7 | OCA1 | OCA2 | Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
596 | 7 | B | 8 | INP52 | INP53 | Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in a TGN (trans Golgi network)-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches
597 | 7 | B | 9 | TOM70 | TOM71 | Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins
598 | 7 | B | 10 | CPT1 | EPT1 | sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability
599 | 7 | B | 11 | [NAME] | RPS9A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Bp and has similarity to E. coli [NAME] and rat S9 ribosomal proteins
600 | 7 | B | 12 | ORM1 | ORM2 | Evolutionarily conserved protein with similarity to Orm1p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum
601 | 7 | C | 1 | DBF2 | [NAME] | Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis
602 | 7 | C | 2 | KEL2 | KEL1 | Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
603 | 7 | C | 3 | SOL4 | SOL3 | 6-phosphogluconolactonase, catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p
604 | 7 | C | 4 | RPL17B | RPL17A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the Dam1 complex (aka DASH complex)
605 | 7 | C | 5 | CWP2 | CWP1 | Cell wall mannoprotein, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; involved in cell wall organization
606 | 7 | C | 6 | FRE2 | [NAME] | Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
607 | 7 | C | 7 | empty | empty
608 | 7 | C | 8 | [NAME] | NET1 | Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase exit; stimulates transcription by [NAME] polymerase I and regulates nucleolar structure
609 | 7 | C | 9 | BCH2 | CHS6 | Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane
610 | 7 | C | 10 | DAL80 | [NAME] | GATA zinc finger protein and Dal80p homolog that negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with [NAME] and binds to Tor1p
611 | 7 | C | 11 | [NAME] | MDV1 | Peripheral protein of the cytosolic face of the mitochondrial outer membrane, required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats
612 | 7 | C | 12 | ASI1 | ASI3 | Putative integral membrane E3 ubiquitin ligase; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
613 | 7 | D | 1 | TBS1 | [NAME] | Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression
614 | 7 | D | 2 | SSE2 | SSE1 | ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm
615 | 7 | D | 3 | YBR270C | BIT61 | Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p-Slm1p-Slm2p), a membrane-associated complex that regulates cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity
616 | 7 | D | 4 | ARE1 | ARE2 | Acyl-CoA:sterol acyltransferase, isozyme of Are1p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen
617 | 7 | D | 5 | TAH1 | PPT1 | Protein serine/threonine phosphatase with similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
618 | 7 | D | 6 | SED4 | SEC12 | Guanine nucleotide exchange factor (GEF); glycosylated integral membrane protein of the endoplasmic reticulum, important for the initiation of transport vesicle budding from the endoplasmic reticulum through activation of the GTPase Sar1p
619 | 7 | D | 7 | SSK22 | SSK2 | MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
620 | 7 | D | 8 | FIG2 | AGA1 | Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds
621 | 7 | D | 9 | RPL21A | RPL21B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Ap and has similarity to rat [NAME] ribosomal protein
622 | 7 | D | 10 | GRX1 | GRX2 | Cytoplasmic glutaredoxin, thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress
623 | 7 | D | 11 | APT1 | APT2 | Apparent pseudogene, not transcribed or translated under normal conditions; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity
624 | 7 | D | 12 | YOX1 | [NAME] | One of two homeobox transcriptional repressors (see also Yox1p), that bind to Mcm1p and to early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval
625 | 7 | E | 1 | PRM6 | YJR054W | Vacuolar protein of unknown function; potential Cdc28p substrate
626 | 7 | E | 2 | [NAME] | HMG2 | One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks
627 | 7 | E | 3 | [NAME] | MMT1 | Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p
628 | 7 | E | 4 | YHR132W-A | YNL157W | Hypothetical protein
629 | 7 | E | 5 | VPH1 | STV1 | Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
630 | 7 | E | 6 | [NAME] | TPO1 | Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane
631 | 7 | E | 7 | [NAME] | YOR227W | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
632 | 7 | E | 8 | YFL042C | YLR072W | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene
633 | 7 | E | 9 | CRP1 | MDG1 | Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations
634 | 7 | E | 10 | SET4 | SET3 | Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains
635 | 7 | E | 11 | MLP1 | MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the [NAME] pathway that controls telomere length
636 | 7 | E | 12 | YKR096W | [NAME] | Putative protein of unknown function, predicted to contain a PINc (PilT N terminus) domain
637 | 7 | F | 1 | VAN1 | ANP1 | Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
638 | 7 | F | 2 | YPK2 | [NAME] | Serine/threonine protein kinase required for receptor-mediated endocytosis; involved in sphingolipid-mediated and cell integrity signaling pathways; localized to the bud neck, cytosol and plasma membrane; homolog of mammalian kinase SGK
639 | 7 | F | 3 | UBC4 | UBC5 | Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins, central component of the cellular stress response; expression is heat inducible
640 | 7 | F | 4 | PHO3 | PHO5 | Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
641 | 7 | F | 5 | PHO5 | PHO3 | Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
642 | 7 | F | 6 | YBR147W | YOL092W | Putative protein of unknown function; predicted to contain six transmembrane domains and is 58% similar to the uncharacterized ORF YBR147W; deletion mutant has no detectable phenotype
643 | 7 | F | 7 | CSH1 | SUR1 | Probable catalytic subunit of a mannosylinositol phosphorylceramide ([NAME]) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p
644 | 7 | F | 8 | SMY2 | YPL105C | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
645 | 7 | F | 9 | YDL012C | YBR016W | Plasma membrane protein of unknown function; has similarity to hydrophilins, which are hydrophilic, glycine-rich proteins involved in the adaptive response to hyperosmotic conditions
646 | 7 | F | 10 | GPM2 | GPM3 | [NAME] of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
647 | 7 | F | 11 | GPD1 | GPD2 | NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria
648 | 7 | F | 12 | PUS9 | RIB2 | DRAP deaminase, catalyzes the third step of the riboflavin biosynthesis pathway; cytoplasmic tRNA pseudouridine synthase involved in pseudouridylation of cytoplasmic tRNAs at position 32
649 | 7 | G | 1 | SIR2 | HST1 | NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
650 | 7 | G | 2 | STP4 | [NAME] | Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids
651 | 7 | G | 3 | RPS29B | RPS29A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Bp and has similarity to rat [NAME] and E. coli S14 ribosomal proteins
652 | 7 | G | 4 | MRK1 | [NAME] | Protein kinase required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta
653 | 7 | G | 5 | ASM4 | NUP53 | Subunit of the nuclear pore complex (NPC), interacts with karyopherin [NAME] or with [NAME] via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex
654 | 7 | G | 6 | THI74 | YML018C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; [NAME] is not an essential gene
655 | 7 | G | 7 | APT2 | APT1 | Adenine phosphoribosyltransferase, catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis
656 | 7 | G | 8 | RPS17B | RPS17A | Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similarity to rat S17 ribosomal protein
657 | 7 | G | 9 | RPS18A | RPS18B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins
658 | 7 | G | 10 | [NAME] | YOX1 | Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate
659 | 7 | G | 11 | TSA2 | TSA1 | Ubiquitous housekeeping thioredoxin peroxidase, reduces reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; mediates redox regulation of the nuclear localization of Yap1p; deletion results in mutator phenotype
660 | 7 | G | 12 | HEH2 | SRC1 | Inner nuclear membrane (INM) protein with a putative role in sister chromatid segregation, potentially phosphorylated by Cdc28p; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence
661 | 7 | H | 1 | empty | empty
662 | 7 | H | 2 | STP1 | STP2 | Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes
663 | 7 | H | 3 | DIG2 | DIG1 | Regulatory protein of unknown function, constitutively-expressed, involved in the regulation of mating-specific genes and the invasive growth pathway, required for MAP-kinase imposed repression, inhibits pheromone-responsive transcription
664 | 7 | H | 4 | PKH1 | PKH2 | Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and [NAME], components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
665 | 7 | H | 5 | ITR1 | ITR2 | Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively
666 | 7 | H | 6 | PLM2 | TOS4 | Transcription factor that binds to a number of promoter regions, particularly promoters of some genes involved in pheromone response and cell cycle; potential Cdc28p substrate; expression is induced in G1 by bound SBF
667 | 7 | H | 7 | PSP1 | YLR177W | Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; YLR177W is not an essential gene
668 | 7 | H | 8 | [NAME] | KCC4 | Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p
669 | 7 | H | 9 | GRX2 | GRX1 | Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair; protects cells from oxidative damage
670 | 7 | H | 10 | EMI2 | GLK1 | Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources
671 | 7 | H | 11 | EUG1 | PDI1 | Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds
672 | 7 | H | 12 | empty | empty
673 | 8 | A | 1 | empty | empty
674 | 8 | A | 2 | RPL1B | RPL1A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal
675 | 8 | A | 3 | FLC3 | FLC1 | [NAME] transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance
676 | 8 | A | 4 | RPL9A | RPL9B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Ap and has similarity to E. coli L6 and rat L9 ribosomal proteins
677 | 8 | A | 5 | RCK1 | RCK2 | Protein kinase involved in the response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations
678 | 8 | A | 6 | TOS3 | SAK1 | Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
679 | 8 | A | 7 | SSA4 | SSA3 | ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm
680 | 8 | A | 8 | [NAME] | APA1 | Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p
681 | 8 | A | 9 | [NAME] | YBR242W | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; [NAME] is not an essential gene
682 | 8 | A | 10 | VPS73 | YBR241C | Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene
683 | 8 | A | 11 | RMD9 | YBR238C | Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
684 | 8 | A | 12 | empty | empty
685 | 8 | B | 1 | SCS3 | [NAME] | Putative protein of unknown function; identified as interacting with Sst2p and Hsp82p in high-throughput two-hybrid screens
686 | 8 | B | 2 | [NAME] | BOI1 | Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain
687 | 8 | B | 3 | RPL23B | RPL23A | Protein component of the large (60S) ribosomal subunit, identical to Rpl23Bp and has similarity to E. coli [NAME] and rat [NAME] ribosomal proteins
688 | 8 | B | 4 | [NAME] | RGM1 | Putative transcriptional repressor with proline-rich zinc fingers; overproduction impairs cell growth
689 | 8 | B | 5 | RPS26B | RPS26A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Bp and has similarity to rat S26 ribosomal protein
690 | 8 | B | 6 | PMD1 | MDS3 | Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions
691 | 8 | B | 7 | YER139C | YDR066C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene
692 | 8 | B | 8 | SPI1 | SED1 | Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites
693 | 8 | B | 9 | [NAME] | RAD34 | Protein involved in nucleotide excision repair (NER); homologous to [NAME]
694 | 8 | B | 10 | DNF1 | DNF2 | Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
695 | 8 | B | 11 | GRX4 | GRX3 | Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage
696 | 8 | B | 12 | BMH1 | BMH2 | 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, [NAME]/MAPK signaling, and rapamycin-sensitive signaling
697 | 8 | C | 1 | DMC1 | RAD51 | Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
698 | 8 | C | 2 | [NAME] | STB6 | Protein that binds Sin3p in a two-hybrid assay
699 | 8 | C | 3 | STV1 | VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes
700 | 8 | C | 4 | FKH1 | [NAME] | Forkhead family transcription factor with a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase
701 | 8 | C | 5 | VHR1 | YER064C | Non-essential nuclear protein; null mutation has global effects on transcription
702 | 8 | C | 6 | RPL31A | RPL31B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similarity to rat [NAME] ribosomal protein; associates with the karyopherin Sxm1p
703 | 8 | C | 7 | KRE2 | KTR1 | Alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family
704 | 8 | C | 8 | empty | empty
705 | 8 | C | 9 | [NAME] | PRO1 | Gamma-glutamyl kinase, catalyzes the first step in proline biosynthesis
706 | 8 | C | 10 | [NAME] | SAM1 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)
707 | 8 | C | 11 | SLF1 | SRO9 | Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif
708 | 8 | C | 12 | PTC2 | PTC3 | Type 2C protein phosphatase; dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA checkpoint inactivation
709 | 8 | D | 1 | VTC2 | VTC3 | Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion
710 | 8 | D | 2 | ENO1 | [NAME] | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose
711 | 8 | D | 3 | [NAME] | TRP3 | Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
712 | 8 | D | 4 | YGR273C | YMR295C | Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; [NAME] is not an essential gene
713 | 8 | D | 5 | HEF3 | YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP
714 | 8 | D | 6 | MSW1 | RSM10 | Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
715 | 8 | D | 7 | RPL2B | RPL2A | Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins
716 | 8 | D | 8 | BUL2 | BUL1 | Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases
717 | 8 | D | 9 | MAD3 | BUB1 | Protein kinase that forms a complex with Mad1p and Bub3p that is crucial in the checkpoint mechanism required to prevent cell cycle progression into anaphase in the presence of spindle damage, associates with centromere DNA via Skp1p
718 | 8 | D | 10 | [NAME] | DIC1 | Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
719 | 8 | D | 11 | ECM33 | PST1 | Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1
720 | 8 | D | 12 | NOT5 | [NAME] | Subunit of the [NAME]-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
721 | 8 | E | 1 | BUD16 | BUD17 | Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
722 | 8 | E | 2 | POR1 | POR2 | Putative mitochondrial porin (voltage-dependent anion channel), related to Por1p but not required for mitochondrial membrane permeability or mitochondrial osmotic stability
723 | 8 | E | 3 | ANP1 | VAN1 | Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
724 | 8 | E | 4 | [NAME] | HTB1 | One of two nearly identical (see [NAME]) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
725 | 8 | E | 5 | [NAME] | TIF4632 | Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains [NAME] ([NAME]); associates with the poly(A)-binding protein [NAME], also interacts with eIF4A (Tif1p); homologous to Tif4631p