Spreadsheet: 2e94ad79-5f76-4e59-9b27-ef96bf625a38
Sheet: Sheet1

Additional File 1:  Details of the SSR markers derived from the BAC sequences and the whole genome sequence spanning the LG I QTL region. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6
Forward and reverse primer sequences and start and end sites for SSR markers are listed with PCR product size in ‘Williams82’ and segregation status in NILs.
ssr_name | forward_primer | reverse_primer | SSR start (Mb) | SSR end (Mb) | Product size in Wms82 | Segregation status
ssrpqtl_1 | TTC TCA CAC ACT TAA CCA | TAA ATC AGG AGG AAC TAT A | 23824808 | 23824866 | 181 | no
ssrpqtl_2 | CTC TAG ATC TGG CAT TTC ACT TAT A | GCA TTG GTC ATA ATC TTG TCG ATG | 23941887 | 23941901 | 134 | no
ssrpqtl_3 | GCC AAG TAT CCA TAC TTA T | GTT AGA TCT GAT GAG CTG TTA | 23988543 | 23988580 | 191 | no
ssrpqtl_4 | TGTGATATGATTTACATATGAATTTTTATGTTTTG | ACCTTACATTCAACATTCAATTTAGGTGCTC | 24812334 | 24812353 | 111 | yes
ssrpqtl_5 | TCCTGTGATGTATATAGTGTTTCAATCATGAAG | GAAATGAGACAGATGTCAAATATCAAAAGATGA | 24971501 | 24971517 | 114 | yes
ssrpqtl_6 | GCTAATGGGATCAGATTTTTATTGTTTTG | ACGAAAATATCATCTAATTTTCATCTTTCTTTT | 25548316 | 25548362 | 193 | yes
ssrpqtl_7 | CAACATCCTAAGGGTCCAAAATAGGAA | GCACCTTAGGTTGCCTAAAATCCACA | 25725062 | 25725131 | 188 | yes
ssrpqtl_8 | GCATTTATATATTCATTGAATTTCGTAGTTTTT | CAATAAACATTTTCAAAATCATGCTAACA | 25751901 | 25751942 | 224 | yes
ssrpqtl_9 | GAGGGATAGCTAGGGGAAAATATGTGTG | GGACAAGTTCAGACATGGATACAAGACA | 26125218 | 26125269 | 128 | yes
ssrpqtl_10 | TCTGATTTATGGAACCAATTGACTGGA | GGACACCATTTCCCTTTTGGGC | 26246206 | 26246235 | 156 | yes
ssrpqtl_11 | ATCACTGTCGTCCTCGCCGTT | AAAATTATGCTAGTTAAGAAGAAAAGATCGGA | 26270814 | 26270846 | 147 | yes
ssrpqtl_12 | AAGCCTGTTGGACTCTTCCAT | AACCATTCTCATCAGTCACACC | 26442066 | 26442101 | 220 | yes
ssrpqtl_13 | ATT GCA CAG GTA TTG ACG | TAT TAC TGC ATA CTA ATA AGG TT | 26444803 | 26444882 | 226 | yes
ssrpqtl_14 | CCC ATC ATT AGA CTA GC | AGT TTT ATT GAT TGG TAG TAT A | 26538403 | 26538420 | 146 | yes
ssrpqtl_15 | CCT CTA TGG TAG CTT AAA T | GAT ATC TAA TTA GCT AGC ACA | 26542454 | 26542492 | 108 | yes
ssrpqtl_16 | GAA GAA TTT CCG TGC CT | GTT GGG AAT TTA TCA GTT CGT | 26609299 | 26609318 | 131 | yes
ssrpqtl_17 | GAA TAT TTG TCA CCG ATA GT | GAG TAA GAG ATT TCA CGT TTG | 26649308 | 26649351 | 170 | yes
ssrpqtl_18 | ACA TTT CTT TAG GCC AAT ATA C | CTA ATA CAT ATA TTG CCA TTG AAG TAA | 26958336 | 26958351 | 100 | yes
ssrpqtl_19 | ATC ACC AAT GTT AGG CTC G | AAG TTT CTT TCC TTA GTT CCC | 27305324 | 27305356 | 105 | yes
ssrpqtl_20 | GTTGTTTTGAAGGCCATGCT | TCACACACGACACCATTTGA | 27877620 | 27877658 | 222 | yes
ssrpqtl_21 | CCCCTCTTCAGCAGATCTCA | TTGCATTTTGGATCCCTTTC | 28275797 | 28275814 | 206 | yes
ssrpqtl_22 | CAAGGTTGACAGGCCACTAA | ACCCCCAGATGATAGCTTCC | 28611270 | 28611287 | 200 | yes
ssrpqtl_23 | GCCGAAACAAATCAATGACA | TGCAAAGGACCATGTGATGT | 28631415 | 28631436 | 201 | yes
ssrpqtl_24 | CATCCCTCCCCTCAAACATA | [NAME] | 29942367 | 29942392 | 174 | yes
ssrpqtl_25 | TGCAAGATCCTGATCGGTAA | GAAAATTGCTCTTTCACGCTTT | 30489918 | 30489971 | 256 | yes
ssrpqtl_26 | AAACAATTCGAGGCAGAGGA | AAAGTTTTGCGGCAAGCAT | 30641540 | 30641560 | 196 | yes
ssrpqtl_27 | CCC CAC TAT TCC TAC TTC TAC CAG | CAA AAT TAA CCT CCC AGC ACT TGT G | 31575661 | 31575726 | 199 | yes
ssrpqtl_28 | CAT TAA AAA TAT CAA TAG AAA TGT G | TGT TTT TGT ACT ATC CAT CG | 31637247 | 31637272 | 109 | yes
ssrpqtl_29 | TTG AAA ATT GAT AAT CGA TCT GAT | GCA GCA GAG AGT TTT GTA GAG TTA | 31787239 | 31787292 | 256 | yes
ssrpqtl_30 | CAC TTT ATT TGA TAT TAC TTG | TAA AAC TAA CAC TAA CGT ATC | 31891817 | 31891846 | 134 | yes
ssrpqtl_31 | ATC ACC ATA ATC AAT TAA ACG | TGT TTT GCT GAT ATG GGT AG | 31978370 | 31978401 | 111 | yes
ssrpqtl_32 | [NAME] GAA TTA TGC TGC ATA T | ATT CCA TCA AAT AAA AGT TGT TAC G | 31992972 | 31993007 | 205 | yes
ssrpqtl_33 | GGG TGA ATT ATA CTA TAT TAC | TTG ATT ACA GAA TAA CCA | 32022042 | 32022063 | 119 | yes
ssrpqtl_34 | GAA ATT CAT GTC CAG AAC | TAC ATC ATA TAC GTT TAT AGA | 32178223 | 32178254 | 138 | yes
ssrpqtl_35 | TAC CAA TAA TAT TGA GGT T | TAT ATA GAA AAC AAT CAA GTG | 32216450 | 32216488 | 176 | yes
ssrpqtl_36 | TTT GCA TAT TTG TTA CTG GCT | TAG TGA TCT ATA CCG GTT AT | 32384780 | 32384811 | 109 | yes
ssrpqtl_37 | [NAME] C | CAT TTC AAG AAA TTT CGA TAC | 32717564 | 32717592 | 134 | yes
ssrpqtl_38 | ATG ATA AAG AGA AAA AGT GAT AGA T | AAG ACA TCA ATG TAC CTC ATC ATT A | 32910185 | 32910244 | 182 | no
ssrpqtl_39 | AAC CTT TTA AAT AAG ATA AGA | AGG GTT TTA TAT TAT GAA G | 32934120 | 32934139 | 177 | no
ssrpqtl_40 | [NAME] ACA AAA GGT CCA AGG | [NAME] GAA ACA ACG GAT | 32936161 | 32936183 | 107 | no
ssrpqtl_41 | TAT TTA TAC CTA GGG TAC TCA | GGC TCA TCC GTT ATG TA | 33048585 | 33048608 | 121 | no
ssrpqtl_42 | GAC AAG AAA TTC TGG ATG GC | TGAATTGAATTGAGAAACGGA | 33091573 | 33091596 | 114 | no