Spreadsheet: 0a4b1248-39ce-4c95-b962-af92725a7a01
Sheet: Sheet1

Unnamed: 0 | readthru | wild type | stem2A | stem2A' | stem2B' | stem2C' | a13465G | stem2C | stem3A | stem3A' | stem3B | stem3C' | deltaS3
 | c1 | f1 | f2 | S2A" | f4 | f5 | a465g | f3 | S3a | S3a" | S3b | S3c" | deltaS3
Maximum | 0.4017921146953405 | 0.1145038167938931 | 0.001841222879684418 | 0.001027101335428123 | 0.0005273069679849341 | 0.002950641658440276 | 0.04845437065522851 | 0.003497544277422235 | 0.02521056388294076 | 0.03666111111111111 | 0.03695568400770713 | 0.04333564333564333 | 0.0641954969796815
q(75) | 0.3915104471572712 | 0.05771492296222252 | 0.001692800516224189 | 0.0009966659989584243 | 0.0004340100797947132 | 0.002887248555404036 | 0.04323755077478562 | 0.003409521404217704 | 0.02146316669208867 | 0.03628708551483421 | 0.03657056145675266 | 0.04152193784277879 | 0.06242603550295858
median, x~ | 0.3832575848044236 | 0.05506072874493927 | 0.001612434411686988 | 0.0009723955292148391 | 0.0003522533322531477 | 0.00286046511627907 | 0.0299756953821226 | 0.003035714285714286 | 0.02028616681947941 | 0.03464665415884928 | 0.03525458248472505 | 0.04070565174779484 | 0.05954545454545455
q(25) | 0.3724077211739178 | 0.05440074517988279 | 0.001496299810246679 | 0.0009397256743230994 | 0.0002519131845019385 | 0.002758036713087747 | 0.02914934885009698 | 0.002787799278451952 | 0.01294827200807131 | 0.03418554006968641 | 0.03379841343910406 | 0.03951434878587196 | 0.0580253723110866
Minimum | 0.3540688575899844 | 0.05272226050999311 | 0.0009157509157509158 | 0.0008998965338851526 | 0.0001870251315020456 | 0.002366594879157693 | 0.0224375 | 0.002635526033854725 | 0.01043074324324324 | 0.03407163441745575 | 0.03250753985351142 | 0.03777982214044771 | 0.05680954964731416
fourth spread | 0.01910272598335339 | 0.003314177782339729 | 0.0001965007059775096 | 5.694032463532492e-05 | 0.0001820968952927747 | 0.0001292118423162885 | 0.01408820192468864 | 0.0006217221257657527 | 0.008514894684017355 | 0.002101545445147797 | 0.0027721480176486 | 0.002007589056906831 | 0.004400663191871976
[NAME] | 0.4119116737794537 | 0.06003199541844886 | 0.001907185470653252 | 0.001057806016167827 | 0.0006253986751923097 | 0.003054282879753502 | 0.05110799826915556 | 0.003968297474362914 | 0.03305850884550544 | 0.03779897232657098 | 0.03941280451119795 | 0.04371703533315508 | 0.06614644933326251
Lower Outlier Boundary | 0.3546034958293935 | 0.05008946207142968 | 0.001317683352720724 | 0.0008869850422618516 | 7.910798931398569e-05 | 0.002666647352804637 | 0.008843392495089645 | 0.002103131097065657 | 0.007513824793453376 | 0.03149433599112758 | 0.03109636045825215 | 0.03769426816243459 | 0.05294445975764658
OUTLIERS? | 1 | 3 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0
mean, xbar | 0.3826415529115957 | 0.05509656369247188 | 0.001618749065031271 | 0.0009680625795526733 | 0.0003471008275988665 | 0.002866806848088667 | 0.03389627138514606 | 0.003055402153391646 | 0.01769228549074623 | 0.03514444795822241 | 0.03503424772030064 | 0.04052361495768736 | 0.0601810665187964
VAR(xbar) | 0.0001608033640977113 | 2.596671881939516e-06 | 1.707770575765671e-08 | 1.782607962513552e-09 | 1.177095454713871e-08 | 3.908233893448199e-09 | 7.902532975816616e-05 | 1.140329796882494e-07 | 2.3833205901574e-05 | 1.149609632652938e-06 | 2.691746829323405e-06 | 2.972165964729113e-06 | 7.560716431971246e-06
STDEV(xbar) | 0.01268082663305951 | 0.001611419213593879 | 0.0001306816963375388 | 4.222094222673804e-05 | 0.0001084940300069027 | 6.251586913295054e-05 | 0.008889619213339015 | 0.0003376876954942975 | 0.004881926453929227 | 0.001072198504313888 | 0.001640654390578164 | 0.001723997089536149 | 0.002749675695781458
se(xbar) | 0.003075552213426466 | 0.0004651766583728807 | 3.169496690203324e-05 | 1.218813618002342e-05 | 2.313100491777876e-05 | 1.804677693626621e-05 | 0.002465536761003359 | 0.0001125625651647658 | 0.001040830217173116 | 0.0003573995014379626 | 0.0005468847968593879 | 0.0005746656965120498 | 0.000916558565260486
Min. [NAME] (N~) | 2 | 2 | 11 | 3 | 151 | 1 | 106 | 19 | 118 | 2 | 4 | 3 | 4
Min. [NAME] (N*) | 7 | 7 | 18 | 8 | 169 | 6 | 124 | 27 | 136 | 7 | 9 | 8 | 9
Actual Sample Size (N) | 17 | 12 | 17 | 12 | 22 | 12 | 13 | 9 | 22 | 9 | 9 | 9 | 9
Enough Sampling? | YES | YES | NO | YES | NO | YES | NO | NO | NO | YES | YES | YES | YES
PPCC | 0.988789065350873 | 0.9834288368566644 | 0.9710192501989022 | 0.9865436933861427 | 0.9675824304890077 | 0.9664780599454097 | 0.9536736045113113 | 0.9637672943560739 | 0.9335369475441553 | 0.9374227604689794 | 0.9729273884245886 | 0.9968076696142113 | 0.9754100264799705
critical value for 0.1alpha | 0.916 | 0.8918 | 0.916 | 0.8918 | 0.9535 | 0.8918 | 0.8974 | 0.8689 | 0.9308 | 0.8689 | 0.8689 | 0.8689 | 0.8689
Normal? | YES | YES | YES | YES | YES | YES | YES | YES | YES | YES | YES | YES | YES
frameshifting, xbar_r |  | 0.1439900169577276 | 0.004230458121220467 | 0.00252994629617848 | 0.0009071174444011823 | 0.007492147223098388 | 0.08858492008309757 | 0.007985024444267703 | 0.04623723000317689 | 0.09184691963222841 | 0.09155892101555119 | 0.1059048988520329 | 0.1572779173115588
se(xbar_r) |  | 0.001678503149787276 | 8.953966200343168e-05 | 3.779018444224504e-05 | 6.088895681084546e-05 | 7.649053148650351e-05 | 0.006482683629382013 | 0.0003010923228939157 | 0.002745388683729181 | 0.001190549987854364 | 0.001607574021777579 | 0.001726299741833992 | 0.002708459741086799
stdev(xbar_r) |  | 0.006364410976707869 | 0.0003691814841222531 | 0.0001385810936352066 | 0.0002851287935250559 | 0.000297223095958125 | 0.02341698759775548 | 0.0009213376152167137 | 0.01285017182935954 | 0.004137224869280438 | 0.005252741638951031 | 0.005711194273665596 | 0.008877296663206899
var(xbar_r) |  | 4.050572708043961e-05 | 1.362949682187094e-07 | 1.920471951312988e-08 | 8.129842889705396e-08 | 8.834156877093275e-08 | 0.0005483553081534342 | 8.488630012132211e-07 | 0.0001651269160440656 | 1.711662961899254e-05 | 2.759129472556996e-05 | 3.261774003155069e-05 | 7.880639604658435e-05
comparing wild type versus mutants
Fold Change |  |  | -0.9706197817695762 | -0.9824297104088736 | -0.993700136554137 | -0.9479675926054101 | -0.3847842930034223 | -0.9445445968201258 | -0.6788858632001469 | -0.3621299478060849 | -0.3641300768619886 | -0.2644983236363921 | 0.09228348349825022
Degrees of Freedom (v) |  |  | 11 | 11 | 11 | 11 | 13 | 11 | 31 | 18 | 18 | 18 | 13
t-statistic |  |  | 75.97991034206554 | 76.97741935099897 | 77.83636434033987 | 74.21389162248093 | 8.208689061383689 | 73.01345830484176 | 29.63396955230681 | 22.69812089778771 | 20.65885892813814 | 14.39516030249524 | 3.815001852784884
p-value |  |  | 2.553658212788674e-16 | 2.212893281081246e-16 | 1.959020594768128e-16 | 3.306055295168192e-16 | 1.684893878155339e-06 | 3.954299530455511e-16 | 2.611671701014953e-24 | 1.072465333241848e-14 | 5.500534671820314e-14 | 2.561375073423884e-11 | 0.002145339803664078
% frameshifting |  | 14.39900169577276 | 0.4230458121220467 | 0.252994629617848 | 0.09071174444011823 | 0.7492147223098388 | 8.858492008309756 | 0.7985024444267703 | 4.623723000317689 | 9.184691963222841 | 9.155892101555118 | 10.59048988520329 | 15.72779173115588
Fold Change |  |  | 0.02938021823042385 | 0.01757028959112644 | 0.006299863445863006 | 0.05203240739458989 | 0.6152157069965776 | 0.05545540317987419 | 0.3211141367998531 | 0.6378700521939151 | 0.6358699231380114 | 0.7355016763636077 | 1.09228348349825
Dataset S1.  Molecular genetic analyses of Stems 2 and 3.  This page provides a summary of the final statistics for the frameshifting experiments shown in figure 6.  Subsequent pages show the raw data and subsequent analyses for all of the different constructs following the methodologies as previously described [46].