Spreadsheet: da5b0a87-3d86-4d67-a201-c27633d4e250
Sheet: Sheet1

Table S3. Quantitative real-time RT-PCR assays for 51 transcripts representing highly significant differences between NOA subclassses | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8
[NAME] | Name | [NAME]. No. | Probe ID | Real-time PCR |  | Assay ID | Primer sequences
 |  |  | (microarray) | (assay) |  |  | Forward (5' -> 3') | Reverse (5' -> 3')
ACAT2 | acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase) | [NAME] | A_23_P383835 | SYBR | TAKARA | HA041816 | GCAAGATGAATGCAGGCTCAGA | CAGCAGCTAAGGCACCATTGAA
ART3 | ADP-ribosyltransferase 3 | NM_001179 | A_23_P80918 | TaqMan | AB | Hs00154151_m1
BUB1 | BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) | NM_004336 | A_23_P124417 | SYBR | TAKARA | HA039468 | GCCATCAAGCCCAAGACTGAA | CCATCAACTGGGTCCCAATGTAG
[NAME] | cell division cycle associated 2 | NM_152562 | A_23_P385861 | SYBR | TAKARA | HA060441 | CCTATTCCCGAGCTGCCTGA | CTTCCAGTTTGCCATTTGAACTGA
CDCA5 | cell division cycle associated 5 | NM_080668 | A_23_P104651 | SYBR | QIAGEN | Hs_CDCA5_SG_1
CDT1 | DNA replication factor | NM_030928 | A_23_P37704 | SYBR | TAKARA | HA054038 | TGGTGGGCAGCTGTTGTACTATC | CTTGTCCAGCTTGACGTAGGTG
CT45-2 | cancer/testis antigen CT45-2 | NM_152582 | A_23_P362076 | SYBR | TAKARA | HA060464 | ACGCTGTGGCTGGCTACTTC | TAGGGCACCTGGGAGTGATG
CTAG1B | cancer/testis antigen 1B | NM_001327 | A_23_P148541 | SYBR | TAKARA | HA035019 | AGAGCCGCCTGCTTGAGTTC | GCAGCAGTCAGTCGGATAGTCAGTA
DAZL | deleted in azoospermia-like | NM_001351 | A_23_P212105 | SYBR | TAKARA | HA035049 | CACCAGTTCAGGTCATCACTGGATA | CAACTTCAGCTCCTGGATCAACTTC
[NAME] | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 | NM_019039 | A_23_P256624 | SYBR | TAKARA | HA050045 | GCTGATCGCATGTTGGATATGG | AGTTGCACTGAACATAAGGGTTTGG
DDX43 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 | NM_018665 | A_23_P156445 | SYBR | TAKARA | HA049687 | AAGCAGACAAGATGTTGGACATGG | GTGCGAGGCGATGAACTGAA
DMRTB1 | DMRT-like family B with proline-rich C-terminal, 1 | NM_033067 | A_23_P417821 | SYBR | TAKARA | HA055770 | CACTGACCGTCCTGTTTGATACTGA | CCTAGTCGGACTGCTCCTGAGA
DMRTC2 | DMRT-like family C2 | NM_033052 | A_23_P300245 | TaqMan | AB | Hs00540564_m1
DSCR8 | Down syndrome critical region gene 8 | NM_032589 | A_23_P6252 | TaqMan | AB | Hs00741223_m1
EGR4 | early growth response 4 | NM_001965 | A_23_P380318 | SYBR | TAKARA | HA035926 | CCATGCTCCACCTTAGCGAGTT | GCTGTTCAAAGCCCAGCTCAA
FGFR3 | fibroblast growth factor receptor 3 ([NAME], thanatophoric dwarfism) | NM_000142 | A_23_P500501 | SYBR | TAKARA | HA031790 | CACCCTACGTTACCGTGCTCAA | AGCCACGCAGAGTGATGAGAA
FMR1NB | fragile X mental retardation 1 neighbor | NM_152578 | A_23_P396112 | SYBR | TAKARA | HA060460 | TCTAAAGATGCGGGTCAGCAAAC | CAGGATATGTCCATTTGCAAGCAC
GAJ | GAJ protein | NM_032117 | A_23_P133123 | SYBR | TAKARA | HA054663 | GTTGATGATGGTATGGTTGACTGTG | CCCTCAGACAACTGAGATTCCAGA
HIST1H2AA | histone 1, H2aa | NM_170745 | A_23_P355439 | SYBR | TAKARA | HA061653 | AGCTAGCGATCCGCAATGATG | TGCCTGAATGTTAGGCAGGACTC
HIST1H2BA | histone 1, H2ba | NM_170610 | A_23_P323821 | TaqMan | AB | Hs00601111_s1
HNRNPG-T | testes-specific heterogenous nuclear ribonucleoprotein G-T | NM_014469 | A_23_P61967 | SYBR | TAKARA | HA045528 | CGAAGCCGAGTTTGGCAAGTA | GGACTTGCCGTTCATGTCTCTG
HORMAD1 | HORMA domain containing protein | NM_032132 | A_23_P327530 | SYBR | TAKARA | HA054688 | CCCAGAATGCGCTTATGGAAC | ACTAACTGTGTAGATCCTGGGCAAT
INHBB | inhibin, beta B (activin AB beta polypeptide) | NM_002193 | A_23_P153964 | SYBR | TAKARA | HA036226 | AAATCATCAGCTTCGCCGAGAC | GACGTAGGGCAGGAGTTTCAGGTA
[NAME] | similar to alpha tubulin | NM_207312 | A_23_P56736 | SYBR | TAKARA | HA026753 | CTGGAAGTAGCGTTGGGCTGA | CATTTGACCATCGGGCTGAA
[NAME] | hypothetical LOC158812 | XM_088679 | A_23_P255457 | SYBR | QIAGEN | Hs_LOC158812_SG_1
LOC255313 | hypothetical protein LOC255313 | NM_173571 | [NAME] | SYBR | TAKARA | HA062398 | CCCATCTTAGGGTGTCGTCTGAG | GACCACGGTGAAGACGGTGA
LOC92196 | similar to death-associated protein | ENST00000309950 | A_23_P165598 | SYBR | QIAGEN | Hs_LOC92196_SG_1
MAGEA2 | melanoma antigen, family A, 2 | NM_005361 | A_23_P148255 | SYBR | QIAGEN | Hs_MAGEA2_SG_1
MGC40042 | similar to mouse meiosis defective 1 gene | AK093341 | A_23_P211561 | TaqMan | AB | Hs00415170_m1
MTL5 | metallothionein-like 5, testis-specific (tesmin) | NM_004923 | A_23_P161507 | SYBR | TAKARA | HA040397 | CATGGCCTGAGCATTGTTTGTC | GCAGCCTCCAGAGCAACATTC
NYD-SP20 | testes development-related NYD-SP20 | NM_032598 | A_23_P101084 | SYBR | TAKARA | HA055218 | TGGCGTGAACATGCACAGAA | TTCGAATAATATGGGCCAGGTGTAA
OTEX | paired-like homeobox protein OTEX | NM_139282 | A_23_P85083 | SYBR | TAKARA | HA058753 | CCGACACACTCAATACCCTGATG | CGCACTTTGTCTTCAGTCACACC
PAGE1 | P antigen family, member 1 (prostate associated) | NM_003785 | A_23_P171104 | TaqMan | AB | Hs00269455_m1
[NAME] | P antigen family, member 5 | NM_130467 | A_23_P22744 | TaqMan | AB | Hs00373098_m1
PEPP-2 | PEPP subfamily gene 2 | AL590524 | A_23_P73677 | SYBR | TAKARA | HA055095 | TCCATCCACACCACTCAGGGTA | [NAME]
PIK3R3 | phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma) | [NAME] | A_23_P22970 | SYBR | QIAGEN | Hs_PIK3R3_SG_1
RAD54L | RAD54-like (S. cerevisiae) | NM_003579 | A_23_P74115 | SYBR | TAKARA | HA038276 | CGAAGCCGTAGCAGTGACAAAG | ATGGACATCGTGCCATCCAG
SPAG5 | sperm associated antigen 5 | NM_006461 | A_23_P89509 | TaqMan | AB | Hs00197708_m1
SSX6 | synovial sarcoma, X breakpoint 6 | NM_173357 | A_23_P33881 | SYBR | QIAGEN | Hs_SSX6_SG_1
STAG3 | stromal antigen 3 | NM_012447 | A_23_P145657 | SYBR | TAKARA | HA044533 | TCCTAGCCGGGTTCTGCAAG | CAGCAGGATTCGGGAACAATG
SYCP3 | synaptonemal complex protein 3 | NM_153694 | A_23_P76374 | SYBR | TAKARA | HA061362 | TTCTGACTTTGTTTCAGCAGTGG | CAATCTCTGGCTCTGAACAATTCTA
TEX14 | testis expressed sequence 14 | NM_031272 | A_23_P54996 | SYBR | TAKARA | HA054180 | AAGCGACTAGGTTGGTCCGAATC | CTGGAACTGCTGCCCTGACA
TKTL1 | transketolase-like 1 | NM_012253 | A_23_P259901 | SYBR | TAKARA | HA044282 | CTCGCTGAGAGCAACATCAACATTA | GAGACGGCATCAGTTGGGTAGAA
TSPY1 | testis specific protein, Y-linked 1 | NM_003308 | A_23_P22800 | SYBR | TAKARA | HA037903 | CTGGCTTCTGGGCCAATGTTA | CATGATCTTGCAGAGATGAACAGGA
[NAME] | testis specific protein, Y-linked 2 | NM_022573 | A_23_P34200 | SYBR | TAKARA | HA052164 | GCCTTCAGGATGGAGGCTGT | AAAGCCGCCTGCTCACTTTC
[NAME] | testis specific protein, Y-linked 2 | NM_022573 | A_23_P251391 | SYBR | TAKARA | HA052164 | GCCTTCAGGATGGAGGCTGT | AAAGCCGCCTGCTCACTTTC
VCX | variable charge, X-linked | NM_013452 | A_23_P252572 | SYBR | TAKARA | HA044908 | AGATGAGTCCAAAGCCGAGAG | TTGGCCACCTTGGTAGTCTTC
VCY | variable charge, Y-linked | NM_004679 | A_23_P11390 | SYBR | TAKARA | HA040018 | GACCGTTGCGAGACGTTGAG | CTCGGCCACCTTGGTAGTCTTC
XAGE1 | X antigen family, member 1 | NM_133431 | A_23_P383310 | SYBR | TAKARA | HA057720 | TCCAACTGGGAGTTGAAGTGTGAG | AGTTATGGCCGTGACGAGACAAG
XAGE3 | X antigen family, member 3 | NM_130776 | A_23_P114343 | SYBR | QIAGEN | Hs_GAGED4_SG_1
XAGE5 | X antigen family, member 5 | NM_130775 | A_23_P301497 | TaqMan | AB | [NAME]