Spreadsheet: c1447fae-0aa7-4028-87fd-ce4bd2f4b619
Sheet: Sheet1

Supplemental Table S5. Comparison of Quantitative RT-PCR data with microarray expression data for genes differentially expressed in P. falciparum and P. vivax sporozoites. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10
[NAME] values are the threshold values determined by [NAME] assay as described in [NAME] and Methods. P. falciparum sporozoite expression values were taken from previously published data (LeRoch, et [NAME]. 2003). QRT-PCR fold change values are equal to 2 raised to the power of the difference in Ct values between the two species.
P. falciparum Gene | P. vivax Gene | Description | P. falciparum  Normalized Avg Ct | P. vivax Normalized Avg Ct | Fold Difference Pf/Pv | Fold difference Pv/Pf | P. falciparum Spz Exp | P. vivax Spz Exp | Fold Difference Pf/Pv | Fold Difference Pv/Pf
PFC0210c | Pv119355 | CSP | 20.871253 | 19.45052333333333 | 0.3735233488203695 | 2.677208809457607 | 28684.1 | 13189 | 2.174850253999545 | 0.4598017717132488
PF11_0545 | Pv092505 | SCOT2 | 25.67718266666667 | 22.521826 | 0.1122387952253258 | 8.90957532101483 | N.D. | 13536 | N.D. | N.D.
PF10_0164 | Pv001715 | UIS4 | 23.595588 | 23.00951033333333 | 0.6661515496910377 | 1.50115990942872 | 10763.7 | 16095 | 0.6687604846225537 | 1.495303659522283
Pf_PKH_141170 | Pv_PKH_141170 | [NAME] | 25.60156866666667 | 24.52611733333334 | 0.4745225874031826 | 2.107381242845539 | N.D. | 17726 | N.D. | N.D.
PFL0650c | Pv123360 | SCOT4 | 32.22470866666666 | 24.66190033333334 | 0.005288930257236067 | 189.0741513620541 | 65 | 2793 | 0.02327246688148944 | 42.96923076923077
PF11_0344 | Pv092275 | AMA1 | 25.995842 | 25.35944533333334 | 0.6433177201660597 | 1.554441870094718 | 1513.4 | 4855 | 0.3117198764160659 | 3.20800845777719
[NAME] | Pv117230 | UIS2 | 28.088442 | 25.98279333333333 | 0.2323467440793395 | 4.303912258217528 | 802.6 | 2332 | 0.3441680960548885 | 2.905556939945178
PF13_0012 | Pv121950 | UIS3 | 30.663247 | 25.61244166666667 | 0.03016866499056591 | 33.14697552287155 | 112.8 | 1082 | 0.1042513863216266 | 9.592198581560284
PF08_0021 | Pv089045 | UVS1 | 35.4001185 | 28.636834 | 0.009205523611836143 | 108.6304312678352 | 20.6 | 5424 | 0.003797935103244838 | 263.3009708737864
[NAME] | Pv086200 | S11 UVS | 39.709528 | 25.72368133333333 | 6.163687880008475e-05 | 16224.05318808299 | 9.5 | 3055 | 0.003109656301145663 | 321.578947368421
PF14_0279 | Pv085040 | UVS | 30.74270933333333 | 26.68308966666667 | 0.05996981503517505 | 16.67505559944539 | 50.3 | 2794 | 0.01800286327845383 | 55.54671968190855
PFD0830w | Pv089950 | DHFR-TS | 32.02608366666666 | 26.2734035 | 0.01854687369326191 | 53.91744272045701 | 10.8 | 2729 | 0.003957493587394651 | 252.6851851851852
MAL13P1.347 | Pv115405 | UVS3 | 28.96020833333333 | 26.88346133333333 | 0.2370483079070334 | 4.218549412266567 | 8.4 | 2696 | 0.003115727002967359 | 320.952380952381
MAL7P1.105 | Pv096306 | [NAME] | 36.556035 | 27.64429266666667 | 0.002076338916915803 | 481.6169421345729 | 4.5 | 1491 | 0.003018108651911469 | 331.3333333333333
PF13_0081 | Pv122540 | UVS | 43.860957 | 26.87374766666667 | 7.697336061669869e-06 | 129915.0760715336 | 36.3 | 111 | 0.327027027027027 | 3.057851239669422
PFB0105c | Pv003535 | PHIST DVS | 24.27322266666667 | 34.16346766666666 | 948.9873197243359 | 0.001053754859749319 | 6534.8 | 94 | 69.51914893617021 | 0.01438452592275203
PF13_0163 | Pv082425 | DVS | 29.86166066666667 | 32.12797033333333 | 4.81090952041439 | 0.2078609035893621 | 3555.1 | 286 | 12.43041958041958 | 0.08044780737531997
PFB0275w | Pv003745 | DVS MFS transporter | 25.83462566666667 | 26.94627366666667 | 2.160923500766277 | 0.4627651092902612 | 2856.2 | 114 | 25.05438596491228 | 0.03991317134654436
PFD0985w | Pv090110 | [NAME] | 26.24438766666667 | 28.76544233333334 | 5.74001563524184 | 0.1742155533271239 | 1194.8 | 200 | 5.974 | 0.1673920321392702
N.D. - Not Determined due to lack of expression data from LeRoch, et [NAME]. 2003
Primers used for Quantitative RT-PCR
Primer Name | Sequence
F-Pv119355 PvCSP | TGCCCCAAATGAAAAGTCTG
R-Pv119355 PvCSP | CCACAGGTTACACTGCATGG
Pf CSP Primer9_F-PFC0210c | TGAATGGTCCCCATGTAGTGT
Pf CSP Primer9_R-PFC0210c | AGCAGAGCCAGGCTTTATTCT
F-Pv001715 UIS4 etramp10.3 | TTTGGATGTGGACGACAGAA
R-Pv001715 UIS4 etramp10.3 | ATAGCCCACTCCACCGAGTA
F-PF10_0164 UIS4 etramp10.3 | TACTGCTGCTGTTGCTTTGG
R-PF10_0164 UIS4 etramp10.3 | GTCGGATCCATCATTCACCT
F-Pv117230 UIS2 | TCTTCCAGTTGTGCATCGAG
R-Pv117230 UIS2 | CAGTCCTACTCCCCCAAGGT
F-PF14_0614 UIS2 | TGAGCGAACTGGTTTCAAAAT
R-PF14_0614 UIS2 | TTCTTTCCATTTGGATTGCTC
F-Pv121950 UIS3 | GCACTGGGAATTGGGTTTTA
R-Pv121950 UIS3 | ACTGGGCACTTGTCCATCTC
F-PF13_0012 UIS3 | TTGATAGCATCTGGGGCAAT
R-PF13_0012 UIS3 | AAATCATCTTCTCGCGATTTTT
F-Pv092275 AMA1 | GAAAAGGGCGGAAATTAAGG
R-Pv092275 AMA1 | ATTTTTCCTCCCCATTTTCG
F-PF11_0344 AMA1 | ATTCGGATTATGGGTCGATG
R-PF11_0344 AMA1 | TCCGAAGCACTCAATTCAAA
F-Pv089950 DHFR-TS | TTAAACTGAACCCGGAGGTG
R-Pv089950 DHFR-TS | CTCCATGGCGATTTTATCGT
F-PFD0830w DHFR-TS | CTGCGCAGTTCATACACGTT
R-PFD0830w DHFR-TS | AAGTGTTGGGAATGGATAGGG
F-Pf_PKH_141170 newSpz1 gene | CAACAAGCCCCTGTCGTTTA
R-Pf_PKH_141170 newSpz1 gene | TTGTGCAGGAACCACATGTAT
F-Pv_PKH141170 newSpz1 gene | GTGTGCACGATTCCACAGAC
R-Pv_PKH141170 newSpz1 gene | GCGTACGCGTAGCTAAGACA
F-PF11_0545 Spz SCOT2 | GGTCAGTAGGCTGTTTTGGTTC
R-PF11_0545 Spz SCOT2 | AACGAGCAGCAGAGGGATAA
F-Pv092505 PvSpz3 SCOT2 | CCTAGGCATGACGTGGAAAT
R-Pv092505 PvSpz3 SCOT2 | ATGTCTCTGGGGTCAAGGTG
F-PFL0650c Spz SCOT4 | TTGCCCCTCTATGTACCAATG
R-PFL0650c Spz SCOT4 | TCTGATTCGTTTTTGACATCAAGT
F-Pv123360 PvSpz24 SCOT4 | TTTCTGGGGCTGAGAAGAAG
R-Pv123360 PvSpz24 SCOT4 | CTCCCACCTCATTTGCCTTA
F-PF14_0279 Spz UVS | TCGTTTCAATTATTTCGCACA
R-PF14_0279 Spz UVS | TGGTTATACATTTACATACCTTTGTTC
F-Pv085040 PvSpz23 UVS | CTTGTCTACACCGCATGCAC
R-Pv085040 PvSpz23 UVS | CTCCAGCACCAGCATCAGTA
F-PF08_0021 UVS1 | AACTTGGGCCCTAATTTTACA
R-PF08_0021 UVS1 | ATACGGTGGGAGGCTCCATA
F-Pv089045 UVS1 | AATTTGGGTCCTCATTTTGC
R-Pv089045 UVS1 | ATCGGTGGCTCCATAAACAC
F-PF13_0081 UVS | TTGGTGGTGTAAAATTCTTGGA
R-PF13_0081 UVS | TTCAGGGTTAATGTCTGGATT
F-PV122540 UVS | GCCAACGACCTCTGTATGGT
R-PV122540 UVS | TCGAGCACCTTCCTGTATCC
F-PF14_0044 Spz S11 UVS | AACCCCTTCAGCTGGATTTT
R-PF14_0044 Spz S11 UVS | ATTTGGGATAACCCTATAAATACAA
F-Pv086200 Spz S11 UVS | ATCAAACCGAAGCAGGTGTC
R-Pv086200 Spz S11 UVS | GTGGGTCCCATCTTCACTTG
F-MAL13P1.347 Spz UVS3 | GCTCTACATCCCCAACCAAA
R-MAL13P1.347 Spz UVS3 | TGTTCGGCATTATTTTGTTCA
F-Pv115405 Spz UVS3 | CAGCAATACAACCCTGCGTA
R-Pv115405 Spz UVS3 | TTCGACGGATAAGTGGAGGT
F-MAL7P1.105 Spz UVS9 | CGAACAGAAAAAGCACAAAAA
R-MAL7P1.105 Spz UVS9 | TCGGATTCATAACCCTCCTG
F-Pv096303new MAL7P1.105 UVS9 | CCGAATGCGTGAGTTCTACA
R-Pv096303new MAL7P1.105 UVS9 | CCCTGGCACTCTGGTACTTC
F-PFB0105c PHIST DVS | TGGTGGAAATGTTGTGGTCA
R-PFB0105c PHIST DVS | AAGAAATTGTTCACGACTTGATGA
F-Pv003535 PHIST DVS | GGGGATTTGTGGAGATTGTG
R-Pv003535 PHIST DVS | CAGCGATTTCTTGCCATTCT
F-PF13_0163 DVS | TGAATTCCTAAAACAAGGTCTGAA
R-PF13_0163 DVS | TTGTCCTCGGGTTGATTAGC
F-Pv082425 DVS | ACTCGGATGACGGAGATGAG
R-Pv082425 DVS | TGATGGCATGAAAAAGGTGA
F-PFB0275w MFS transporter | GGAGCTCCTTTGTTTGGTTATTT
R-PFB0275w MFS transporter | TGTTTTCGATAAGGCTTGTGC
F-Pv003745 MFS transporter | AGCCTGCTCCACTTCACCTA
R-Pv003745 MFS transporter | CTCGTCCTCGTCGTCGTACT
F-PFD0985w AP2 DVS | AGGGCTAGCACAAGCAAAAA
R-PFD0985w AP2 DVS | CCTCCTCTTTCGAGAATTCAA
F-Pv090110 AP2 DVS | CGCAAGAGGAGGATCAACTC
R-Pv090110 AP2 DVS | CTTGCCACTGTTCGTGTTGT