Spreadsheet: 03004f94-84c5-4b09-acce-2d9285f61aac
Sheet: List1

Significantly regulated proteins identified in 2-DE experiment including spot number, protein name, Swiss-Prot database number, number of peptides matched to the identified protein, number of unassigned peaks, sequence coverage, [NAME] score of the identified protein, [NAME] score for the highest ranked hit to a non-homologous protein, peptide sequences confirmed by MS/MS ([NAME] score given in parenthesis), MW and pI.
S1(A) | Differentially expressed proteins in pH range 4-7 of the 2-DE experiment
Spot No. | Protein name | Accession No. | No. matching peptides | Umatched peaks | Sequence coverage (%) | [NAME] score Top hit | [NAME] score 2nd hit | Peptide sequence ([NAME] score) | MW (kDa) | pI
4 | L-lactate dehydrogenase B chain | P07195 | 15 | 3 | 40 | 181 | 26 | No | 37 | 5.7
7 | Rho GDP-dissociation inhibitor 2 | P52566 | 9 | 4 | 48 | 115 | 40 | No | 23 | 5.1
11 | Elongation factor 1-gamma | P26641 | 11 | 5 | 26 | 126 | 45 | No | 50 | 6.3
12 | Protein disulfide-isomerase A3 | P30101 | 18 | 3 | 38 | 241 | 46 | No | 57 | 6
22 | Heterogeneous nuclear ribonucleoprotein F | P52597 | 9 | 4 | 29 | 107 | 36 | No | 46 | 5.4
24 | [NAME], mitochondrial | P38646 | 26 | 1 | 47 | 348 | 41 | No | 74 | 5.9
25 | 60S acidic ribosomal protein P0 | P05388 | 14 | 2 | 51 | 218 | 56 | No | 34 | 5.7
29 | Heat shock 70 kDa protein 1A/1B | P08107 | 17 | 2 | 30 | 226 | 43 | No | 70 | 5.5
35 | T-complex protein 1 subunit beta | P78371 | 18 | 4 | 48 | 231 | 53 | No | 57 | 6
36 | Stathmin | P16949 | 9 | 3 | 44 | 120 | 39 | No | 17 | 5.8
41 | Gamma-enolase | P09104 | 8 | 2 | 30 | 118 | 43 | No | 47 | 4.9
45 | Peptidyl-prolyl cis-trans isomerase FKBP4 | Q02790 | 18 | 5 | 52 | 241 | 77 | No | 52 | 5.4
64 | 60 kDa heat shock protein, mitochondrial | P10809 | 11 | 2 | 27 | 119 | 24 | No | 61 | 5.7
68 | T-complex protein 1 subunit gamma | P49368 | 15 | 5 | 28 | 141 | 48 | No | 60 | 6.1
70 | Actin, cytoplasmic 1 | P60709 | 17 | 2 | 53 | 224 | 114 | No | 42 | 5.3
83 | Activator of 90 kDa heat shock protein ATPase homolog 1 | O95433 | 14 | 2 | 45 | 213 | 47 | No | 38 | 5.4
91 | DNA replication licensing factor [NAME] | P3399 | 10 | 2 | 19 | 116 | 35 | SLEQNIQLPAALLSR (37) | 81 | 6.1
97 | Cytochrome b-c1 complex subunit 1, mitochondrial | [NAME] | 16 | 5 | 33 | 163 | 30 | No | 53 | 5.9
116 | RuvB-like 1 | Q9Y265 | 11 | 2 | 35 | 158 | 39 | ALESSIAPIVIFASNR (56) | 50 | 6
125 | Heat shock 70 kDa protein 4 | P34932 | 16 | 3 | 26 | 147 | 32 | No | 94 | 5.1
126 | Cofilin-1 | [NAME] | 16 | 4 | 71 | 213 | 33 | No | 19 | 8.2
131 | 60 kDa heat shock protein, mitochondrial | P10809 | 14 | 2 | 28 | 171 | 39 | No | 61 | 5.7
136 | Transaldolase | P37837 | 15 | 1 | 46 | 231 | 43 | No | 38 | 6.4
140 | 78 [NAME] glucose-regulated protein | P11021 | 12 | 1 | 26 | 175 | 31 | No | 72 | 5
141 | Caprin-1 | Q14444 | 8 | 5 | 11 | 81 | 52 | GKLDDYQER (43) | 78 | 5.1
144 | Prohibitin | P35232 | 11 | 2 | 60 | 187 | 47 | No | 30 | 5.6
153 | D-3-phosphoglycerate dehydrogenase | O43175 | 11 | 1 | 28 | 155 | 31 | EELIAELQDCEGLIVR (57) ALQSGQCAGAALDVFTEEPPRDR (57) | 57 | 6.3
159 | [NAME]-coenzyme A transferase 1, mitochondrial | P55809 | 8 | 2 | 23 | 94 | 28 | No | 56 | 7.1
166 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial | P10515 | 8 | 1 | 15 | 108 | 40 | VVDGAVGAQWLAEFR (94) | 67 | 8.0
220 | Heterogeneous nuclear ribonucleoprotein H | P31943 | 10 | 2 | 25 | 121 | 35 | No | 49 | 5.9
221 | Plastin-2 | P13796 | 13 | 2 | 25 | 142 | 44 | No | 70 | 5.3
223 | Mitochondrial inner membrane protein | [NAME] | 17 | 2 | 27 | 206 | 51 | No | 84 | 6.1
230 | Proteasome subunit alpha type-5 | P28066 | 8 | 3 | 48 | 132 | 45 | No | 26 | 4.7
233 | Rab GDP dissociation inhibitor alpha | P84103 | 12 | 5 | 32 | 148 | 43 | No | 50 | 5
242 | Splicing factor, arginine/serine-rich 3 | P84103 | 6 | 2 | 37 | 103 | 36 | NPPGFAFVEFEDPR (35) | 19 | 11.6
244 | Heterogeneous nuclear ribonucleoprotein H | P31943 | 10 | 2 | 37 | 148 | 35 | No | 49 | 5.9
267 | Hematopoietic lineage cell-specific protein | P14317 | 6 | 1 | 18 | 85 | 40 | EHPVPLLPIR (37) | 54 | 4.7
278 | Spermidine synthase | P19623 | 8 | 1 | 33 | 129 | 35 | No | 34 | 5.3
279 | Protein disulfide-isomerase A3 | P30101 | 8 | 2 | 24 | 104 | 49 | No | 57 | 6
282 | Ubiquitin-like modifier-activating enzyme 1 | P22314 | 15 | 3 | 18 | 161 | 45 | No | 118 | 5.5
287 | DnaJ homolog subfamily A member 2 | O60884 | 9 | 3 | 20 | 109 | 34 | No | 46 | 6.1
289 | Galactokinase | P51570 | 7 | 1 | 27 | 106 | 37 | No | 42 | 6
291 | Peptidyl-prolyl cis-trans isomerase FKBP5 | Q13451 | 5 | 2 | 17 | 64 | 41 | EEQCILYLGPR (42) | 51 | 5.7
295 | Dynactin subunit 2 | [NAME] | 14 | 4 | 40 | 167 | 36 | No | 44 | 5.1
297 | Heat shock 70 kDa protein 1A/1B | P08107 | 8 | 2 | 16 | 88 | 41 | No | 70 | 5.5
308 | F-actin-capping protein subunit alpha-2 | P47755 | 12 | 2 | 62 | 193 | 43 | No | 33 | 5.6
311 | Protein disulfide-isomerase A3 | P30101 | 9 | 2 | 22 | 119 | 46 | No | 57 | 6.0
318 | Splicing factor, arginine/serine-rich 7 | [NAME] | 10 | 4 | 34 | 142 | 47 | NPPGFAFVEFEDPRDAEDAVR (37) | 27 | 11.8
320 | Heat shock protein 75 kDa, mitochondrial | Q12931 | 10 | 2 | 19 | 124 | 44 | YESSALPSGQLTSLSEYASR (99) | 80 | 8.3
346 | Transitional endoplasmic reticulum ATPase | P55072 | 11 | 1 | 16 | 104 | 28 | No | 89 | 5.1
360 | Mitochondrial-processing peptidase subunit alpha | Q10713 | 11 | 5 | 25 | 119 | 30 | No | 58 | 6.5
364 | 4-trimethylaminobutyraldehyde dehydrogenase | P49189 | 6 | 3 | 17 | 67 | 39 | ANDTTFGLAAGVFTR (80)   [NAME] (40) | 54 | 5.7
382 | [NAME] | P08758 | 6 | 3 | 24 | 87 | 68 | QVYEEEYGSSLEDDVVGDTSGYYQR (158) | 36 | 4.9
399 | [NAME] | Q6FI81 | 6 | 2 | 24 | 75 | 25 | EPLTPEEVQSVR (30) | 34 | 5.4
407 | Protein phosphatase methylesterase 1 | Q9Y570 | 6 | 2 | 19 | 81 | 39 | LPSRPPLPGSGGSQSGAK (41) | 42 | 5.7
413 | NADH-ubiquinone oxidoreductase 75 [NAME] subunit, mitochondrial | P28331 | 12 | 1 | 20 | 156 | 28 | No | 79 | 5.9
424 | ATP synthase subunit beta, mitochondrial | P06576 | 12 | 2 | 28 | 148 | 26 | No | 57 | 5.3
428 | Quinone oxidoreductase-like protein 1 | O95825 | 11 | 4 | 48 | 163 | 48 | No | 39 | 5.5
437 | L-lactate dehydrogenase B chain | P07195 | 7 | 4 | 26 | 100 | 36 | VIGSGCNLDSAR (46) | 37 | 5.7
442 | Serine/threonine-protein phosphatase 2A 65 [NAME] regulatory subunit A alpha isoform | P30153 | 8 | 1 | 14 | 111 | 44 | No | 65 | 5
455 | G-rich sequence factor 1 | Q12849 | 8 | 1 | 20 | 95 | 41 | No | 53 | 5.8
461 | Rho GDP-dissociation inhibitor 2 | P52566 | 13 | 7 | 65 | 180 | 45 | No | 23 | 5.1
466 | Eukaryotic translation initiation factor 3 subunit G | O75821 | 7 | 4 | 24 | 88 | 65 | GFAFISFHR (31) ETDLQELFRPFGSISR (38) | 35 | 5.9
526 | Rab GDP dissociation inhibitor beta | P50395 | 11 | 5 | 35 | 145 | 36 | No | 51 | 6.1
541 | Heat shock protein 105 kDa | Q92598 | 8 | 1 | 12 | 91 | 41 | No | 97 | 5.3
561 | Gamma-enolase | P09104 | 7 | 3 | 20 | 93 | 47 | No | 47 | 4.9
569 | SUMO-activating enzyme subunit 2 | Q9UBT2 | 10 | 3 | 22 | 124 | 37 | YLFNQLFGEEDADQEVSPDR (59) QFILVMNALDNR (41) | 71 | 5.2
573 | 60 kDa heat shock protein, mitochondrial | P10809 | 10 | 2 | 21 | 99 | 48 | No | 61 | 5.7
574 | TAR DNA-binding protein 43 | Q13148 | 4 | 1 | 11 | 53 | 36 | FGGNPGGFGNQGGFGNSR (118) | 45 | 5.9
583 | Dual specificity mitogen-activated protein kinase kinase 2 | P36507 | 7 | 4 | 25 | 88 | 49 | No | 44 | 6.1
592 | Ezrin | P15311 | 10 | 1 | 18 | 131 | 36 | No | 69 | 5.9
595 | L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase | Q9NRN7 | 6 | 2 | 20 | 79 | 41 | No | 36 | 6.4
597 | [NAME]-coenzyme A transferase 1, mitochondrial | P55809 | 8 | 4 | 26 | 91 | 35 | [NAME] (48) | 56 | 7.1
615 | Transformer-2 protein homolog beta | P62995 | 5 | 5 | 23 | 64 | 39 | [NAME] (53) | 34 | 11.3
630 | Ubiquitin-like domain-containing CTD phosphatase 1 | Q8WVY7 | 7 | 1 | 30 | 106 | 36 | NTIMFDDIGR (26) | 37 | 6.1
635 | Semenogelin-1 | P04279 | 7 | 1 | 15 | 99 | 55 | No | 52 | 9.3
642 | Paraspeckle component 1 | Q8WXF1 | 6 | 1 | 17 | 74 | 41 | GFVEFAAKPPAR (37)         FAQPGTFEFEYASR (41) | 59 | 6.3
645 | Gametocyte-specific factor 1 | Q8WW33 | 7 | 2 | 37 | 120 | 33 | SCIEQDVVNQTR (45) LLQCPYDKNHQIR (37) | 19 | 6
647 | Mitochondrial inner membrane protein | [NAME] | 14 | 4 | 23 | 163 | 50 | No | 84 | 6.1
671 | Charged multivesicular body protein 2a | O43633 | 6 | 4 | 18 | 73 | 63 | KAEAAASALADADADLEER  (80)                            Ac-MDLLFGR (33) IMMEFER (35) | 25 | 5.9
679 | Stathmin | P16949 | 6 | 1 | 40 | 99 | 43 | [NAME] (46) | 17 | 5.8
699 | Rho GDP-dissociation inhibitor 2 | P52566 | 6 | 4 | 37 | 93 | 34 | No | 23 | 5.1
702 | 4-trimethylaminobutyraldehyde dehydrogenase | P49189 | 6 | 4 | 15 | 62 | 36 | ANDTTFGLAAGVFTR (82) | 54 | 5.7
768 | Glutathione synthetase | P48637 | 9 | 2 | 22 | 114 | 26 | No | 52 | 5.7
783 | Serine/threonine-protein phosphatase 2A 65 [NAME] regulatory subunit A beta isoform | P30154 | 7 | 3 | 14 | 83 | 53 | LASGDWFTSR (55) TSACGLFSVCYPR (78) | 66 | 4.8
787 | Glutathione S-transferase P | P09211 | 6 | 5 | 44 | 92 | 35 | No | 23 | 5.4
791 | Phosphoserine phosphatase | P78330 | 7 | 1 | 57 | 131 | 34 | No | 25 | 5.5
801 | Hypoxia up-regulated protein 1 | Q9Y4L1 | 8 | 1 | 9 | 88 | 38 | DAVVYPILVEFTR (75) | 111 | 5.2
849 | Heterogeneous nuclear ribonucleoprotein F | P52597 | 6 | 2 | 20 | 76 | 35 | No | 46 | 5.4
908 | GTP-binding protein SAR1b | Q9Y6B6 | 5 | 4 | 34 | 79 | 32 | IDRPEAISEER (45) [NAME] (39) | 22 | 5.8
1025 | Serine/threonine-protein kinase 3 | Q13188 | 7 | 4 | 21 | 87 | 37 | AIFMIPTNPPPTFR (49) | 56 | 5.1
1033 | Signal transducing adapter molecule 1 | Q92783 | 6 | 2 | 17 | 74 | 56 | PLFATNPFDQDVEK (50) | 59 | 4.7
1050 | Adenylosuccinate synthetase isozyme 2 | [NAME] | 9 | 1 | 28 | 131 | 42 | No | 50 | 6.1
1415 | Crk-like protein | P46109 | 8 | 2 | 36 | 128 | 48 | No | 34 | 6.3
S1(B) | Differentially expressed proteins in pH range 6-11 of the 2-DE experiment
Spot No. | Protein name | Accession No. | No. matching peptides | Umatched peaks | Sequence coverage (%) | [NAME] score Top hit | [NAME] score 2nd hit | Peptide sequence ([NAME] score) | MW (kDa) | pI
4b | Elongation factor 2 | P13639 | 16 | 5 | 17 | 146 | 46 | No | 95 | 6.4
8b | Phosphoglycerate kinase 1 | P00558 | 15 | 6 | 49 | 177 | 39 | No | 45 | 8.3
15b | Heterogeneous nuclear ribonucleoprotein H3 | P31942 | 15 | 1 | 63 | 232 | 45 | No | 37 | 6.4
22b | Glucose-6-phosphate isomerase | P06744 | 11 | 1 | 21 | 120 | 51 | No | 63 | 8.4
25b | Peptidyl-prolyl cis-trans isomerase | P62937 | 18 | 5 | 59 | 183 | 42 | No | 18 | 7.7
30b | Staphylococcal nuclease domain-containing protein 1 | Q7KZF4 | 9 | 1 | 12 | 107 | 42 | No | 102 | 6.7
33b | C-1-tetrahydrofolate synthase, cytoplasmic | P11586 | 9 | 2 | 10 | 98 | 36 | [NAME] (42) [NAME] (42) | 101 | 6.9
34b | Inosine-5'-monophosphate dehydrogenase 2 | P12268 | 9 | 2 | 17 | 125 | 46 | No | 56 | 6.4
36b | Peptidyl-prolyl cis-trans isomerase A | P62937 | 11 | 3 | 55 | 155 | 41 | No | 18 | 7.7
37b | C-1-tetrahydrofolate synthase, cytoplasmic | P11586 | 19 | 5 | 22 | 201 | 41 | No | 101 | 6.9
44b | Far upstream element-binding protein 2 | Q92945 | 12 | 1 | 31 | 175 | 34 | No | 73 | 6.8
46b | GMP synthase [glutamine-hydrolyzing] | P49915 | 10 | 3 | 18 | 115 | 41 | [NAME] (47) | 77 | 6.4
53b | Poly(rC)-binding protein 1 | Q15365 | 9 | 2 | 42 | 141 | 58 | No | 37 | 6.7
61b | KH domain-containing, RNA-binding, signal transduction-associated protein 1 | Q07666 | 11 | 4 | 24 | 112 | 31 | No | 48 | 8.7
63b | EH domain-containing protein 1 | Q9H4M9 | 8 | 1 | 16 | 110 | 34 | HLIEQDFPGMR (22) [NAME] (35) | 61 | 6.4
72b | Proteasome subunit beta type-2 | P49721 | 5 | 1 | 36 | 82 | 34 | No | 23 | 6.5
85b | DAZ-associated protein 1 | [NAME] | 5 | 1 | 18 | 76 | 46 | No | 43 | 8.7
96b | Obg-like ATPase 1 | [NAME] | 8 | 2 | 23 | 105 | 33 | No | 45 | 7.6
107b | Glucose-6-phosphate 1-dehydrogenase | P11413 | 6 | 2 | 15 | 78 | 37 | No | 59 | 6.4
109b | Heterogeneous nuclear ribonucleoproteins A2/B1 | P22626 | 9 | 2 | 33 | 139 | 36 | No | 37 | 9.0
110b | ATP-dependent RNA helicase DDX1 | Q92499 | 8 | 3 | 16 | 90 | 30 | No | 82 | 6.8
115b | Elongation factor 2 | P13639 | 11 | 4 | 16 | 109 | 49 | No | 95 | 6.4
124b | S-formylglutathione hydrolase | P10768 | 7 | 1 | 39 | 109 | 32 | No | 31 | 6.5
128b | Eukaryotic translation initiation factor 2 subunit 3 | Q2VIR3 | 4 | 1 | 10 | 54 | 41 | VGQEIEVRPGIVSK (47)  AGGEAGVTLGQPHLSR (63) LIGWGQIR (26) | 51 | 8.7
129b | Polyribonucleotide nucleotidyltransferase 1, mitochondrial | Q8TCS8 | 9 | 3 | 11 | 112 | 40 | No | 86 | 7.9
140b | Far upstream element-binding protein 2 | Q92945 | 12 | 1 | 33 | 187 | 24 | No | 73 | 6.8
163b | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | P11310 | 9 | 1 | 23 | 130 | 32 | No | 46 | 8.6
170b | Far upstream element-binding protein 2 | Q92945 | 7 | 1 | 16 | 94 | 41 | No | 73 | 6.8
209b | Phosphoglycerate kinase 1 | P00558 | 13 | 4 | 43 | 175 | 51 | No | 45 | 8.3
289b | Lamina-associated polypeptide 2, isoform alpha | P42166 | 7 | 1 | 15 | 93 | 30 | FQETEFLSPPR (37) | 75 | 7.6
334b | Electron transfer flavoprotein subunit beta | P38117 | 7 | 3 | 31 | 97 | 35 | No | 28 | 8.2
341b | Phosphoglycerate kinase 1 | P00558 | 5 | 2 | 18 | 67 | 38 | No | 45 | 8.3
S1(C) | Differentially expressed proteins in pH ranges 4-7 and 6-11 of the 2-DE experiment with two proteins identified in one spot
Spot No. | Protein name | Accession No. | No. matching peptides | Umatched peaks | Sequence coverage (%) | [NAME] score Top hit | [NAME] score 2nd hit | Peptide sequence ([NAME] score) | MW (kDa) | pI
387 | Sorting nexin-6 | Q9UNH7 | 4 | 2 | 10 | 50 | 37 | TFLLEYHNR (52) TVAMHEVFLCR (52) | 47 | 5.8
387 | Heterogeneous nuclear ribonucleoprotein H | P31943 | 8 | 5 | 28 | 105 | 31 | ATENDIYNFFSPLNPVR (53) [NAME] (57) | 49 | 5.9
577 | COP9 signalosome complex subunit 8 | Q99627 | 4 | 4 | 24 | 61 | 44 | SANSELGGIWSVGQR (66) | 23 | 5.3
577 | Chromobox protein homolog 3 | Q13185 | 3 | 1 | 22 | 50 | 34 | CPQIVIAFYEER (60) | 21 | 5.2
612 | Proteasome activator complex subunit 1 | Q06323 | 10 | 8 | 37 | 118 | 64 | VDVFREDLCTK (29) | 29 | 5.8
612 | Peflin | Q9UBV8 | 7 | 2 | 22 | 64 | 24 | NLFQQYDR (37) LSFEDFVTMTASR (62) | 30 | 6.1
803 | N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 | O95865 | 10 | 11 | 42 | 129 | 60 | DFAVSTVPVSGPSHLR (66) AGAGLSSLCLVLSTRPHS (46) | 30 | 5.7
803 | F-actin-capping protein subunit beta | P47756 | 6 | 5 | 21 | 60 | 48 | KLEVEANNAFDQYR (37) | 31 | 5.4
840 | Proteasome activator complex subunit 1 | Q06323 | 7 | 5 | 27 | 83 | 52 | No | 29 | 5.8
840 | Glutathione S-transferase P | P09211 | 4 | 1 | 29 | 52 | 37 | No | 23 | 5.4
83b | Septin-6 | Q14141 | 14 | 5 | 30 | 101 | 41 | No | 50 | 6.2
83b | Tubulin beta chain | P07437 | 12 | 19 | 31 | 103 | 101 | No | 50 | 4.8
150b | Adenylosuccinate lyase | P30566 | 5 | 5 | 11 | 50 | 41 | AAGGDHGSPDSYR (67) AAGGDHGSPDSYRSPLASR (56) | 55 | 6.7
150b | Fascin | Q16658 | 4 | 1 | 8 | 39 | 33 | LINRPIIVFR (53) | 55 | 6.8