Spreadsheet: aa8f88bd-c740-4a2a-9eac-bd0054dcce9c
Sheet: Sheet1

Name | Use | Sequence
SN-2 | bridging primer for all deletions | ccgctgctaggcgcgccgtgACCAGTGTGATGGATATCTGC
SN-5 | bridging primer for all deletions | gcagggatgcggccgctgacAGCTCGGATCCACTAGTAACG
ERG2-del-upF | Upstream deletion fragment | AGTAATGGTTGCTCCGGTTG
ERG2-del-upR | Upstream deletion fragment | CACGGCGCGCCTAGCAGCGGTGATACGTTTCGGTTCTCTGTC
ERG2-del-dnF | Downstream deletion fragment | GTCAGCGGCCGCATCCCTGCTAATAGAGGGGGCACGGATT
ERG2-del-dnR | Downstream deletion fragment | ATCCATGGCAACAAATGAAA
ERG2-del-upcheck | Check insertion of deletion | AATCGTATCCCTTCGTCGTC
ERG2-intcheck-F | checking loss of endogenous gene | CCACGGCCATTATGATTGAT
ERG2-intcheck-R | checking loss of endogenous gene | GATTGATCCAGTACCAGCATGA
[NAME] | Upstream deletion fragment | CCTTTGCATTTGCTAAAATCTG
[NAME] | Upstream deletion fragment | CACGGCGCGCCTAGCAGCGGAAAATTGGCTAAACCGAATCC
[NAME] | Downstream deletion fragment | GTCAGCGGCCGCATCCCTGCCATCTTTGTTTTGGACCATTGA
[NAME] | Downstream deletion fragment | CAAAGCCAACCAGATCATCA
[NAME] | Check insertion of deletion | TGTGCAGGAATGCAATTGTT
ERG3-intcheck-F | checking loss of endogenous gene | GATTTCCCAAGTCTCCCAAA
ERG3-intcheck-R | checking loss of endogenous gene | GGAAGAACCCATCAACTGGA
ERG4-del-upF | Upstream deletion fragment | AAT GAC GTC TCC CCC TCT TT
ERG4-del-upR | Upstream deletion fragment | CAC GGC GCG CCT AGC AGC GGT GTT GAT GGT TGT AAA ATG AAA AA
ERG4-del-dnF | Downstream deletion fragment | GTC AGC GGC CGC ATC CCT GCT TGC TAA GCT ACA TAA TAT CAA AAT CA
ERG4-del-dnR | Downstream deletion fragment | CCA CTG GTC CAA AAC AAC AA
ERG4-del-upcheck | Check insertion of deletion | CCA CTG GTC CAA AAC AAC AA
ERG4-intcheck-F | checking loss of endogenous gene | GCT GGG GTT CCA TTC AGT TA
ERG4-intcheck-R | checking loss of endogenous gene | AAT GGA GAT CCA AAC CCA CA
ERG5-del-upF | Upstream deletion fragment | GCAACGCGTAACAACACACT
ERG5-del-upR | Upstream deletion fragment | CACGGCGCGCCTAGCAGCGGAAAGTGAATGTTTTGTGGTTTTTG
ERG5-del-dnF | Downstream deletion fragment | GTCAGCGGCCGCATCCCTGCGAGGGAAATAAGTAGGTCAAAACAA
ERG5-del-dnR | Downstream deletion fragment | CGAGGTTGCTCCATCAATTT
ERG5-del-upcheck | Check insertion of deletion | GTTCCGGTTGGATCGAATTA
ERG5-intcheck-F | checking loss of endogenous gene | TGCTTCATCTCGTGATTTGG
ERG5-intcheck-R | checking loss of endogenous gene | AATGATCGGGAAATTGACCA
ERG6-del-upF | Upstream deletion fragment | CGATGGCCGTAGTTCTTACA
ERG6-del-upR | Upstream deletion fragment | CACGGCGCGCCTAGCAGCGGGAAAATGAAAGGTTCTTTAACTTGA
ERG6-del-dnF | Downstream deletion fragment | GTCAGCGGCCGCATCCCTGCGGGGCTTGACAAACAACAAG
ERG6-del-dnR | Downstream deletion fragment | GCTAAGCCCATTTTCAGTGG
ERG6-del-upcheck | Check insertion of deletion | CCGAAATATCATTGGGAACG
ERG6-intcheck-F | checking loss of endogenous gene | GTGGTGTAGGTGGTCCTGGT
ERG6-intcheck-R | checking loss of endogenous gene | GTTGTTTGTCAAGCCCCATT
ERG11-del-upF | Upstream deletion fragment | TTTGAGAACAGCCACACGAC
ERG11-del-upR | Upstream deletion fragment | CACGGCGCGCCTAGCAGCGGTGAGTTATGATCTTCTTGAAAAGAAAC
ERG11-del-dnF | Downstream deletion fragment | GTCAGCGGCCGCATCCCTGCCGGCAACTTTCTTTCGATTC
ERG11-del-dnR | Downstream deletion fragment | AGCAGATGATGCTGGAACCT
ERG11-del-upcheck | Check insertion of deletion | GCCGGGAATAACTGAGAAAA
ERG11-intcheck-F | checking loss of endogenous gene | TTTGACCGTTCATTTGCTCA
ERG11-intcheck-R | checking loss of endogenous gene | TAGCTTTGGCAGCAGCAGTA
ERG24-del-upF | Upstream deletion fragment | TTGAACCCCATCCAAATTGT
ERG24-del-upR | Upstream deletion fragment | CACGGCGCGCCTAGCAGCGGAACTTTGGTTAGTTATTTTGTGGAAA
ERG24-del-dnF | Downstream deletion fragment | [NAME]
ERG24-del-dnR | Downstream deletion fragment | AAATGATGCCGAAATCGAAC
ERG24-del-upcheck | Check insertion of deletion | AAGGTTTCCAGACATTCCACA
ERG24-intcheck-F | checking loss of endogenous gene | TGGCTGGCCTATTTAGCTTG
ERG24-intcheck-R | checking loss of endogenous gene | ACGACCAATGAACCAATCGT
CNB1-del-upF | Upstream deletion fragment | TTCTGGTGGCTCATTCTTTG
CNB1-del-upR | Upstream deletion fragment | GGGAAGAGAATTGAACAGGTTG
CNB1-del-dnF | Downstream deletion fragment | CACGGCGCGCCTAGCAGCGGTTGTCAGTAGTTGTTGAAGTCGAA
CNB1-del-dnR | Downstream deletion fragment | GTCAGCGGCCGCATCCCTGCCAACAGTTTTGGTTTAGTTCATGT
CNB1-del-upcheck | Check insertion of deletion | TTGTTGAGCAGGTTGCTGTC
CNB1-intcheck-F | checking loss of endogenous gene | TAAAGATGGGTCAGGGCAAA
CNB1-intcheck-R | checking loss of endogenous gene | CGTCCAAATCAGCTTCCATT
YHB1-RT-F | RTPCR | [NAME]
YHB1-RT-R | RTPCR | TCCTTGTCTGTAGCTGGGTCATAGA
RBT5-RT-F | RTPCR | [NAME]
RBT5-RT-R | RTPCR | AGCCTTAGCCAAAGCGTCA
FET34-RT-F | RTPCR | ATGAGGAGGAGGAAGAACAAAGC
FET34-RT-R | RTPCR | GGGCTAGAAGAGGAACCAGTTG
FTR1-RT-F | RTPCR | TATCATTGCCACGGTCTTGA
FTR1-RT-R | RTPCR | GGACCAGAACCGTTTTCAGA
[NAME] | RTPCR | CCCAACTGAATCTGATGACCTTAC
[NAME] | RTPCR | GCCAACACAGCAGCATTTAC
SIT1-RT-F | RTPCR | CTGCCAGTCAACCCGTCTATG
SIT1-RT-R | RTPCR | AACTCCAAACGACCAAGGACAT
CRH11-RT-F | RTPCR | CCAGTTCTTCATCCAGCTCA
CRH11-RT-R | RTPCR | CCAATCAATGCAACAAAGCC
[NAME] | RTPCR | TTCGGTAAAGACGACGACAAAGA
[NAME] | RTPCR | GCCAAATGAAGAGGATCCATAAGA
STP4-RT-F | RTPCR | TCCTTTCAAGAACATCGATTCA
STP4-RT-R | RTPCR | TTATGCATCCAATCATCGACA
CAT1-RT-F | RTPCR | TTG GTC AAC ACG GTC CAT T
CAT1-RT-R | RTPCR | CCA TAA GCA CCG GAA CCT T
GPX1-RT-F | RTPCR | ATG GCA AGA ACC AGG CAC TA
GPX1-RT-R | RTPCR | CTG GAT CTG CTT GTT CAC CA
UTR2-RT-F | RTPCR | CCATGCTGTTCTCAATTTGGTA
UTR2-RT-R | RTPCR | TCTAGGCATAGGCATACAAGCA
[NAME] | RTPCR | AAG AGC CAC ACA AGC GAT TT
[NAME] | RTPCR | [NAME]
ECM331-RT-F | RTPCR | GAGTATTCTCAACTCCCCATCG
ECM331-RT-R | RTPCR | AAATCGCAAAGGAAGGTAATGA
TEF3-RT-F | RTPCR | CCA CTG AAG TCA AGT CCG TTG A
TEF3-RT-R | RTPCR | CAC CTT CAG CCA ATT GTT CGT
[NAME] | RTPCR | GCT TTT GGT GTT TGA CGA GTT TCT
[NAME] | RTPCR | GTG AGC CGG GAA ATC TGT ATA GTC
TDH3-RT-F | RTPCR | ATC CCA CAA GGA CTG GAG A
TDH3-RT-R | RTPCR | GCA GAA GCT TTA GCA ACG TG
RPP2B-RT-F | RTPCR | ACT TAG CTG CTT ACT TAT TGT TAG
RPP2B-RT-R | RTPCR | GTC TTT ACC TTC CAA ATC TTT CA