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Table S1. Geographic locations of materials used in this study, list of accessions used for each analysis, and the results of the PCR-based genotyping. | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10 | Unnamed: 11 | Unnamed: 12 | Unnamed: 13 | Unnamed: 14 | Unnamed: 15 | Unnamed: 16 | Unnamed: 17 | Unnamed: 18 | Unnamed: 19
No. | Species | Geographic location | Population |  | Latitude |  | Longitude | AkSRK-A | Insertion§ | AkSRK-B | AkSRK-C | AkSRK-D | AkSRK-E |  | Clustering‡ | Full length‡‡ | [NAME] | Crossing** | F2††
1 | Arabidopsis kamchatica subsp. kamchatica | Taiwan | [NAME] | N | 24.02 | E | 121.33 | yes | no | no | no | yes | no |  | y
2 | Arabidopsis kamchatica subsp. kamchatica | Taiwan | [NAME] | N | 24.02 | E | 121.33 | yes | no | no | no | yes | no |  | y
3 | Arabidopsis kamchatica subsp. kamchatica | Taiwan | [NAME] | N | 24.02 | E | 121.28 | yes | no | no | no | yes | no |  | y
4 | Arabidopsis kamchatica subsp. [NAME] | Lowland in Western Honshu, Japan | Mie, [NAME], Fukiiura, 2 m | N | 34.6 | E | 136.6 | yes | yes | no | no | yes | no |  | y
5 | Arabidopsis kamchatica subsp. [NAME] | Lowland in Western Honshu, Japan | Mie, [NAME], Fukiiura, 2 m | N | 34.6 | E | 136.6 | yes | yes | no | no | yes | no |  | y
6 | Arabidopsis kamchatica subsp. [NAME] | Lowland in Western Honshu, Japan | Shiga, Takashima, 85m ([NAME]) | N | 35.45 | E | 136.06 | yes | yes | no | no | yes | no |  | y
7 | Arabidopsis kamchatica subsp. [NAME] | Lowland in Western Honshu, Japan | Shiga, Ohtsu, Ohmimaiko, 85 m | N | 35.24 | E | 135.95 | yes | yes | no | no | yes | no |  | y
8 | Arabidopsis kamchatica subsp. [NAME] | Lowland in Western Honshu, Japan | Shiga, Hikone, 85 m | N | 35.23 | E | 136.17 | yes | yes | no | no | yes | no |  | y
9 | Arabidopsis kamchatica subsp. [NAME] | Lowland in Western Honshu, Japan | Toyama, Hamakurosaki, 2 m | N | 36.78 | E | 137.27 | yes | yes | no | no | yes | no |  | y
10 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Western Honshu and Shikoku, Japan | Tokushima, Mt. Shikokutsurugi, 1740 m | N | 33.86 | E | 134.09 | yes | no | no | no | yes | no |  | y
11 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Western Honshu and Shikoku, Japan | [NAME], [NAME], 580 m | N | 35.39 | E | 133.53 | yes | no | no | no | yes | no |  | y
12 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Western Honshu and Shikoku, Japan | Tottori, Mt. Daisen, 1600 m | N | 35.37 | E | 133.53 | yes | no | no | no | yes | no |  | y
13 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, along Jintsu River at Toyama airport, 30 m | N | 36.65 | E | 137.18 | yes | no | no | no | yes §§ | no |  | y
14 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Ishikawa, Mt. Hakusan, Ichinose, 1080 m | N | 36.12 | E | 136.73 | yes | no | no | no | yes | no |  | y
15 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Mt. Shirouma, 2800 m | N | 36.76 | E | 137.76 | no | NP | yes | no | yes | no |  | y
16 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Tsurugigozen, 2740 m | N | 36.58 | E | 137.6 | yes | no | no | no | yes | no |  | y |  |  | y
17 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Tateyama, Mikurigaike, 2400 m | N | 36.58 | E | 137.6 | yes | no | no | no | yes | no |  | y
18 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Tateyama, [NAME], 2400 m | N | 36.58 | E | 137.6 | yes | no | no | no | yes | no |  | y
19 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Kurobe-dam, 1500 m | N | 36.57 | E | 137.66 | yes | no | no | no | yes | no |  | y
20 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Nagano, Kamikochi, Myojin, 1520 m | N | 36.25 | E | 137.66 | yes | no | no | no | yes | no |  | y
21 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Nagano, Kamikochi, Shimomatashirodani-deai, 1570 m | N | 36.26 | E | 137.69 | yes | no | no | no | yes | no |  | y
22 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Yamanashi, Mt. Kitadake, 3090 m | N | 35.67 | E | 138.24 | yes | no | no | no | yes | no |  | y
23 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Shizuoka, Mt. Fuji, Subashiri, 1300 m | N | 35.36 | E | 138.81 | yes | no | no | no | yes | no |  | y
24 | Arabidopsis kamchatica subsp. kamchatica | Lowland in Northern Honshu, Japan | Niigata, Tsugawa | N | 37.69 | E | 139.44 | yes | no | no | no | yes | no |  | y
25 | Arabidopsis kamchatica subsp. kamchatica | Hokkaido, Japan | Takinoue | N | 44.19 | E | 143.08 | no | no | no | no | yes | no |  | y
26 | Arabidopsis kamchatica subsp. kamchatica | Hokkaido, Japan | Asahikawa, Sounkyo, 640 m | N | 43.73 | E | 142.95 | no | NP | yes | no | yes | no |  | y
27 | Arabidopsis kamchatica subsp. kamchatica | Hokkaido, Japan | Kushiro, Obirashike, 20 m | N | 43.03 | E | 144.48 | no | NP | no | no | yes | no |  | y
28 | Arabidopsis kamchatica subsp. kamchatica | Hokkaido, Japan | Kushiro, Kombumori, 5 m | N | 42.95 | E | 144.53 | no | NP | no | no | yes | no |  | y
29 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, Nachiki, basin of the Nachikinskoe ozero lake, 400 m | N | 53.07 | E | 157.77 | no | NP | yes | no | yes | no |  | y
30 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, near the road from Petropavlovsk Kamchatskii to Esso, 260 m | N | 53.22 | E | 157 | no | NP | yes | no | yes | no |  | y
31 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, close to the bridge over the river Vaktan Malkinskii, 300 m | N | 53.49 | E | 157.56 | no | NP | no | yes | yes | no |  | y
32 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, close to the bridge over the river Denokhonok, 265 m | N | 54.27 | E | 158.12 | no | NP | yes | no | yes | no |  | y
33 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, [NAME], Mishenaya gora, 10 m | N | 53.01 | E | 158.61 | no | NP | yes | yes | yes | no |  | y
34 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, Elizovo, 70 m | N | 53.19 | E | 158.5 | no | NP | yes | no | yes | no |  | y
35 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, Nachiki, Mt. Nachikinskoe zerkaltse, 730 m | N | 53.12 | E | 157.77 | no | NP | yes | no | no | yes |  | y
36 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, [NAME], Avachinskaya sopka, 640 m | N | 53.19 | E | 158.78 | no | NP | yes | no | yes | no |  | y
37 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, close to the bridge over the river Vaktan Ganal’skii, 320 m | N | 53.53 | E | 157.61 | no | NP | no | yes | yes | no |  | y
38 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, close to the bridge over the river Pravaya Kamchatka, 500 m | N | 54.02 | E | 157.85 | no | NP | yes | no | yes | no |  | y
39 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula, Russia | [NAME] krai, Esso, Srednii kamchatskii khrebe, 910 m | N | 55.91 | E | 158.76 | no | NP | yes | no | no | yes |  | y
40 | Arabidopsis kamchatica subsp. kamchatica | Alaska, USA | Alaska, Kenai, 300 m | N | 60.18 | W | 149.65 | no | NP | yes | no | yes | no |  | y
41 | Arabidopsis kamchatica subsp. kamchatica | Alaska, USA | Alaska, Chugach State Park, Potter, 5 m | N | 61.06 | W | 149.81 | no | NP | yes | no | yes | no |  | y | AkSRK-B, D | y | y | y
42 | Arabidopsis kamchatica subsp. kamchatica | Alaska, USA | Alaska, Chena River, Chena Hot Springs Rd. | N | 64.92 | W | 146.28 | no | NP | yes | no | yes | no |  | y
43 | Arabidopsis kamchatica subsp. kamchatica | Alaska, USA | Alaska, [NAME], South of Darling Creek bridge | N | 63.42 | W | 145.84 | no | NP | yes | no | yes | no |  | y |  |  | y
44 | Arabidopsis kamchatica subsp. kamchatica | Alaska, USA | Alaska, Portage Bay Rd | N | 60.82 | W | 148.99 | no | NP | yes | no | yes | no |  | y
45 | Arabidopsis kamchatica subsp. kamchatica | Canada and Washington | Canada, Yukon, [NAME], 670 m | N | 60.3 | W | 137.54 | no | NP | yes | no | yes | no |  | y
46 | Arabidopsis kamchatica subsp. kamchatica | Canada and Washington | USA, Washington, Mt. [NAME] | N | 48.8 | W | 121.88 | no | NP | yes | no | yes | no |  | y
47 | Arabidopsis kamchatica subsp. kamchatica | Canada and Washington | USA, Washington, Mt. [NAME] | N | 48.8 | W | 121.88 | no | NP | yes | no | yes | no |  | y
48 | Arabidopsis kamchatica subsp. kamchatica | Okhotsk, Russia | Khabarovsk krai, Okhotskii raion, foothills of the Lanzhinskie gory Mountains | N | 59.39 | E | 143.32 | no | NP | yes | no | no | yes |  | y | AkSRK-B | y
49 | Arabidopsis kamchatica subsp. kamchatica | Okhotsk, Russia | Khabarovsk krai, Okhotskii raion, [NAME] gory Mountains | N | 59.39 | E | 143.32 | no | NP | yes | no | no | yes |  | y | AkSRK-B | y
Accessions that were not used for the distribution analysis, but for crossings, the full-length sequence analysis and/or the F2 analysis.
50 | Arabidopsis kamchatica subsp. kamchatica | Taiwan | [NAME] | N | 24.02 | E | 121.28 | yes | no | no | no | yes | no |  |  | AkSRK-A, D¶ | y
51 | Arabidopsis kamchatica subsp. [NAME] | Lowland in Western Honshu, Japan | Shiga, Takashima, 85m ([NAME]) | N | 35.45 | E | 136.06 | yes | yes | no | no | yes | no |  |  | AkSRK-A, D | y | y | y
52 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Western Honshu and Shikoku, Japan | Tokushima, Mt. Shikokutsurugi, 1740 m | N | 33.86 | E | 134.09 | yes | no | no | no | yes | no |  |  | AkSRK-D | y
53 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Murodo | N | 36.58 | E | 137.6 | yes | no | no | no | yes | no |  |  |  |  | y
54 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Murodo | N | 36.58 | E | 137.6 | yes | no | no | no | yes | no |  |  |  |  | y
55 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Murodo | N | 36.58 | E | 137.6 | yes | no | no | no | yes | no |  |  | AkSRK-A, D | y
56 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Tsurugigozen, 2740 m | N | 36.76 | E | 137.76 | yes | no | no | no | yes | no |  |  |  |  | y
57 | Arabidopsis kamchatica subsp. kamchatica | Mountains in Central Honshu, Japan | Toyama, Tsurugigozen, 2740 m | N | 36.76 | E | 137.76 | yes | no | no | no | yes | no |  |  |  |  | y
58 | Arabidopsis kamchatica subsp. kamchatica | Kamchatka Peninsula | [NAME] krai, close to the bridge over the river Denokhonok, 265 m | N | 54.27 | E | 158.12 | no | NP | no | yes | yes | no |  |  | AkSRK-C¶ | y
59 | Arabidopsis kamchatica subsp. kamchatica | Okhotsk, Russia | Khabarovsk krai, Okhotskii raion, foothills of the Lanzhinskie gory Mountains | N | 59.39 | E | 143.32 | no | NP | yes | no | no | yes |  |  | AkSRK-E¶ | y
60 | Arabidopsis kamchatica subsp. kamchatica | Okhotsk, Russia | Khabarovsk krai, Okhotskii raion, foothills of the Lanzhinskie gory Mountains | N | 59.39 | E | 143.32 | no | NP | yes | no | no | yes |  |  | AkSRK-B¶
61 | Arabidopsis halleri | Lowland in Western Honshu, Japan | Osaka, [NAME] | N | 34.9 | E | 135.35 |  |  |  |  |  |  |  |  | AhSRK-B | y | y
62 | Arabidopsis halleri | Lowland in Western Honshu, Japan | Shiga, Mt. Ibuki | N | 35.42 | E | 136.4 |  |  |  |  |  |  |  |  | AhSRK26-Ibuki |  | y
63 | Arabidopsis halleri | Lowland in Western Honshu, Japan | Shiga, Mt. Ibuki | N | 35.42 | E | 136.4 |  |  |  |  |  |  |  |  | AhSRK01-Ibuki | y | y
64 | Arabidopsis halleri | Switzerland | Boden | N | 46.67 | E | 8.27 |  |  |  |  |  |  |  |  |  |  | y
65 | Arabidopsis halleri | Switzerland | Beride | N | 46 | E | 8.83 |  |  |  |  |  |  |  |  |  |  | y
Columns "AkSRK-A", "AkSRK-B", "AKSRK-C", "AkSRK-D" and "AkSRK-E" are results of the PCR-based genotyping. "yes" indicates the amplification and "no" indicates no amplification. NP: not performed.
§ Genotyping for the ~1,700-bp insertion in AkSRK-A. ‡ Used for the [NAME] and STRUCTURE analysis. ‡‡ Accessions where full-lengh SRK were obtained. ¶ Used for the prediction of amino acid sequences (Figure S3). † Used for calculating nucleotide divergence from A. halleri and A. lyrata. ** Used for the interspecific crossing experiment. †† Used for the F2 segregation analysis.
§§ Amplification was not observed by a PCR using AkSRK-D specific primers (13-42genF4 and 13-42R4b; see Table S3). Then we designed an another primer pair at a different position based on the full-length sequence of AkSRK-D (13-42F4 and 13-42R6; see Table S3), which successfully amplified [NAME]