Spreadsheet: 268726d1380756613-help-circumference-error-circumference-error
Sheet: Table 6 unproc stab (% and MFI)

Time=0
 | Relative Percentage
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 54.9 | 11 | 22.8 | 29.2 | 18.2 | 93.4
MF39735M | 61.1 | 9.4 | 17.9 | 64.1 | 18.3 | 97.4
MF39746M | 61.4 | 5.6 | 15.9 | 34.6 | 13.5 | 89.8
MF39758F | 70 | 14.9 | 13.8 | 55.8 | 13.6 | 95.4
MF39755F | 56.7 | 28.2 | 17.4 | 38.5 | 24.5 | 94.9
MF39754F | 64.8 | 22.7 | 23.2 | 67.9 | 8.1 | 94.8
MF39756F | 61.6 | 7.9 | 26.5 | 34.7 | 6.6 | 78.1
MF39757F | 65.8 | 21 | 21.6 | 44.7 | 10.2 | 87.5
MF39743M | 72 | 3.9 | 10.9 | 35.8 | 9.8 | 91.8
MF39720M | 68 | 9.1 | 23.8 | 29.9 | 4.3 | 90.8
Mean | 63.6 | 13.4 | 19.4 | 43.5 | 12.7 | 91.4
SD | 5.5 | 8.1 | 5 | 14.2 | 6.2 | 5.5
Time=24
 | Relative Percentage
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 55.5 | 5.3 | 21.8 | 9.3 | 14.4 | 90.6
MF39735M | 61.4 | 3.7 | 17.4 | 47.7 | 16.8 | 98.8
MF39746M | 68.9 | 4.2 | 16.2 | 25.8 | 10.8 | 90.6
MF39758F | 71.9 | 9.4 | 12.2 | 25.7 | 11.7 | 95
MF39755F | 57.3 | 18.8 | 16.9 | 18.9 | 22.4 | 93
MF39754F | 65 | 20.7 | 22.9 | 56.9 | 5.6 | 98
MF39756F | 62.4 | 7.3 | 25.7 | 34 | 5.6 | 80
MF39757F | 64.6 | 17.7 | 23.3 | 40.1 | 9.5 | 91.2
MF39743M | 71.1 | 2.5 | 11.3 | 26.5 | 12.4 | 90.5
MF39720M | 68.3 | 7.4 | 24.2 | 28.3 | 4.6 | 85.4
Mean | 64.6 | 9.7 | 19.2 | 31.3 | 11.4 | 91.3
SD | 5.6 | 6.8 | 5.1 | 13.9 | 5.6 | 5.6
Time=48
 | Relative Percentage
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 54.9 | 7.8 | 20.3 | 10.9 | 11.7 | 93.9
MF39735M | 60.5 | 4.5 | 16.2 | 49.6 | 15.3 | 98
MF39746M | 66.8 | 6.2 | 15.6 | 31.1 | 9.2 | 91.5
MF39758F | 69.9 | 14.1 | 12.2 | 34.2 | 11 | 97.3
MF39755F | 57.2 | 28.1 | 16 | 26.3 | 22.6 | 95.2
MF39754F | 64.4 | 27.1 | 22 | 66.3 | 4.7 | 97
MF39756F | 62.3 | 10.1 | 25.7 | 45.4 | 5.1 | 84.4
MF39757F | 65.9 | 29.7 | 21.4 | 55.9 | 10 | 90.8
MF39743M | 71.6 | 3.4 | 10.3 | 39.8 | 8.2 | 91.9
MF39720M | 68.2 | 10.8 | 23.2 | 36.9 | 3.4 | 88.7
Mean | 64.2 | 14.2 | 18.3 | 39.6 | 10.1 | 92.9
SD | 5.4 | 10.2 | 5 | 15.7 | 5.7 | 4.3
 | SUMMARY
 | Mean (Relative Percentage)
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
0 hr | 63.6 | 13.4 | 19.4 | 43.5 | 12.7 | 91.4
24 hr | 64.6 | 9.7 | 19.2 | 31.3 | 11.4 | 91.3
48 hr | 64.2 | 14.2 | 18.3 | 39.6 | 10.1 | 92.9
 | % Difference
0 vs 24 hr | 1.6 | 32 | 1 | 32.6 | 10.8 | 0.1
Pass/Fail | Pass | Fail | Pass | Fail | Pass | Pass
 | % Difference
0 vs 48 hr | 0.9 | 5.8 | 5.8 | 9.4 | 22.8 | 1.6
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
Time=0
 | Mean Fluorescence Intensity of AF488
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 352 | 1427 | 416 | 940 | 1977 | 2093
MF39735M | 346 | 1404 | 1088 | 1560 | 5190 | 5320
MF39746M | 253 | 1286 | 535 | 1204 | 4172 | 4617
MF39758F | 438 | 1457 | 850 | 1308 | 3366 | 3517
MF39755F | 588 | 1342 | 613 | 1181 | 2607 | 2731
MF39754F | 548 | 1485 | 1253 | 1580 | 3915 | 4111
MF39756F | 267 | 1157 | 645 | 1233 | 1804 | 2241
MF39757F | 644 | 1661 | 991 | 1634 | 4909 | 5596
MF39743M | 282 | 2168 | 738 | 1430 | 3840 | 4164
MF39720M | 342 | 1454 | 631 | 1360 | 2817 | 3058
Mean | 406 | 1484 | 776 | 1343 | 3460 | 3745
SD | 141 | 274 | 265 | 215 | 1157 | 1226
Time=24
 | Mean Fluorescence Intensity of AF488
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 309 | 1430 | 368 | 1122 | 1753 | 1881
MF39735M | 318 | 1647 | 825 | 1328 | 4334 | 4382
MF39746M | 247 | 1112 | 453 | 1058 | 3322 | 3639
MF39758F | 353 | 1280 | 634 | 1402 | 2715 | 2840
MF39755F | 496 | 1225 | 590 | 1581 | 2146 | 2272
MF39754F | 459 | 1201 | 909 | 1332 | 3305 | 3365
MF39756F | 267 | 1008 | 611 | 1106 | 1486 | 1794
MF39757F | 591 | 1518 | 852 | 1403 | 4034 | 4400
MF39743M | 262 | 1811 | 625 | 1271 | 2787 | 3047
MF39720M | 323 | 1284 | 600 | 1189 | 2115 | 2388
Mean | 362.5 | 1351.6 | 646.7 | 1279.2 | 2799.7 | 3000.8
SD | 114.7 | 248.6 | 171.7 | 162.8 | 950.5 | 945.8
CD3/CD20/CD16/TSF19
Time=48
 | Mean Fluorescence Intensity of AF488
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 343 | 1473 | 404 | 1211 | 2058 | 2152
MF39735M | 333 | 1582 | 854 | 1353 | 5129 | 5223
MF39746M | 270 | 1145 | 551 | 1194 | 4160 | 4520
MF39758F | 441 | 1389 | 767 | 1475 | 3406 | 3494
MF39755F | 619 | 1361 | 683 | 1447 | 2640 | 2744
MF39754F | 588 | 1291 | 1087 | 1461 | 4002 | 4115
MF39756F | 311 | 1070 | 744 | 1189 | 1751 | 2018
MF39757F | 790 | 1648 | 1136 | 1609 | 5059 | 5551
MF39743M | 295 | 1733 | 755 | 1260 | 3246 | 3508
MF39720M | 384 | 1361 | 710 | 1256 | 2564 | 2822
Mean | 437.4 | 1405.3 | 769.1 | 1345.5 | 3401.5 | 3614.7
SD | 172.2 | 210.7 | 220 | 145.6 | 1178.6 | 1225.1
 | SUMMARY
 | Mean Fluorescence Intensity of AF488
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
0 hr | 406 | 1484 | 776 | 1343 | 3460 | 3745
24 hr | 363 | 1352 | 647 | 1279 | 2800 | 3001
48 hr | 437 | 1405 | 769 | 1346 | 3402 | 3615
 | % Difference
0 vs 24 hr | 11 | 9 | 18 | 5 | 21 | 22
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
 | % Difference
0 vs 48 hr | 7 | 5 | 1 | 0 | 2 | 4
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
Time=0
 | Absolute Count
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 2260.233 | 249 | 938.676 | 274 | 749.294 | 700
MF39735M | 5512.442 | 518 | 1614.938 | 1035 | 1651.026 | 1608
MF39746M | 2290.834 | 128 | 593.229 | 205 | 504 | 453
MF39758F | 4534.599999999999 | 676 | 893.964 | 499 | 881.008 | 840
MF39755F | 2484.027 | 700 | 762.294 | 293 | 1073 | 1018
MF39754F | 3959.928 | 899 | 1417.752 | 963 | 494.991 | 469
MF39756F | 6280.12 | 496 | 2702 | 938 | 672.87 | 526
MF39757F | 3887.463999999999 | 816 | 1276.128 | 570 | 603 | 528
MF39743M | 4967.28 | 194 | 751.991 | 269 | 676.102 | 621
MF39720M | 3972.56 | 362 | 1390.396 | 416 | 251.206 | 228
Mean | 4015 | 504 | 1234 | 546 | 756 | 699
SD | 1373 | 268 | 617 | 319 | 386 | 387
Time=24
 | Absolute Count
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 2284.935 | 121 | 898 | 84 | 592.8480000000001 | 537
MF39735M | 5539.508 | 205 | 1569.828 | 749 | 1515.696 | 1498
MF39746M | 2570.659 | 108 | 604.422 | 156 | 402.948 | 365
MF39758F | 4657.682000000001 | 438 | 790.316 | 203 | 757.9259999999999 | 720
MF39755F | 2510.313 | 472 | 740.3889999999999 | 140 | 981 | 912
MF39754F | 3972.15 | 822 | 1399.419 | 796 | 342.216 | 335
MF39756F | 6361.68 | 464 | 2620.115 | 891 | 570.92 | 457
MF39757F | 3816.567999999999 | 676 | 1376.564 | 552 | 561.26 | 512
MF39743M | 4905.188999999999 | 123 | 779.587 | 207 | 855.476 | 774
MF39720M | 3990.086 | 295 | 1413.764 | 400 | 269 | 230
Mean | 4061 | 372 | 1219 | 418 | 685 | 634
SD | 1350 | 246 | 603 | 306 | 368 | 370
Time=48
 | Absolute Count
Animal | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
MF39741M | 2260.233 | 176 | 835.7510000000001 | 91 | 481.689 | 452
MF39735M | 5458.309999999999 | 246 | 1461.564 | 725 | 1380.366 | 1353
MF39746M | 2492.308 | 155 | 582.036 | 181 | 343.252 | 314
MF39758F | 4528.122 | 638 | 790.316 | 270 | 712.58 | 693
MF39755F | 2505.932 | 704 | 700.96 | 184 | 990.106 | 943
MF39754F | 3935.484 | 1067 | 1344.42 | 891 | 287 | 278
MF39756F | 6351.485 | 641 | 2620.115 | 1190 | 519.9449999999999 | 439
MF39757F | 3893.372 | 1156 | 1264.312 | 707 | 590.8000000000001 | 536
MF39743M | 4939.684 | 168 | 710.5970000000001 | 283 | 565.718 | 520
MF39720M | 3984.244 | 430 | 1355.344 | 500 | 198.628 | 176
Mean | 4035 | 538 | 1167 | 502 | 607 | 570
SD | 1348 | 368 | 605 | 364 | 353 | 351
 | SUMMARY
 | Mean (Absolute Count)
 | CD3+ | CD3+/TSF19+ | CD3-/CD20+ | CD3-/CD20+/TSF19+ | CD3-/CD20-/CD16+ | CD3-/CD20-/CD16+/TSF19+
0 hr | 4015 | 504 | 1234 | 546 | 756 | 699
24 hr | 4061 | 372 | 1219 | 418 | 685 | 634
48 hr | 4035 | 538 | 1167 | 502 | 607 | 570
 | % Difference
0 vs 24 hr | 1.1 | 30.1 | 1.2 | 26.6 | 9.9 | 9.8
Pass/Fail | Pass | Fail | Pass | Pass | Pass | Pass
 | % Difference
0 vs 48 hr | 0.5 | 6.5 | 5.6 | 8.4 | 21.9 | 20.3
Pass/Fail | Pass | Pass | Pass | Pass | Pass | Pass
 | Calculations for Lymphocyte Count
Animal | # of Events R1 |  | # of Events R2 |  | # of Beads Per Test | Test Volume | Total
MF39741M | 7168 |  | 1762 |  | 50602 | 50 | 4117
MF39735M | 8032 |  | 901 |  | 50602 | 50 | 9022
MF39746M | 6871 |  | 1864 |  | 50602 | 50 | 3731
MF39758F | 7969 |  | 1245 |  | 50602 | 50 | 6478
MF39755F | 7437 |  | 1718 |  | 50602 | 50 | 4381
MF39754F | 7808 |  | 1293 |  | 50602 | 50 | 6111
MF39756F | 8341 |  | 828 |  | 50602 | 50 | 10195
MF39757F | 7682 |  | 1316 |  | 50602 | 50 | 5908
MF39743M | 7846 |  | 1151 |  | 50602 | 50 | 6899
MF39720M | 7868 |  | 1363 |  | 50602 | 50 | 5842