Spreadsheet: 036d999d-fc14-4655-9c63-abc1ceb12880
Sheet: Sheet1

Table S1. Yeast strains used in this study. | Unnamed: 1
Strain | Relevant Genotype*
SLJ2039 | Mata [NAME] mps3Δ::NATMX pURA3-MPS3
SLJ0844 | Mata bar1- kar1Δ17 pURA3-KAR1
SLJ0894 | Mata bar1- cdc31-2 pURA3-CDC31
SLJ0718 | Mata [NAME]-1
SLJ1558 | Mata sfi1-3
[NAME] | Mata CDC31-16 pURA3-CDC31
[NAME] | Mata mps3Δ::NATMX ura3::mps3Δ75-150-GFP-URA3 htb2::HTB2-mCherry-HYGMX
SLJ4308 | Mata mps3Δ::NATMX ura3::mps3∆TM-GFP-URA3 htb2::HTB2-mCherry-KANMX
SLJ4246 | Mata mps3Δ::NATMX ura3::mps3Δcc1-GFP-URA3 htb2::HTB2-mCherry-KANMX
SLJ4248 | Mata mps3Δ::NATMX ura3::mps3Δcc2-GFP-URA3 htb2::HTB2-mCherry-KANMX
SLJ1239 | Mata mps3Δ::HIS3 leu2::mps3ΔpQ-GFP-KANMX-LEU2
SLJ3428 | [NAME] ura3::mps3-2xpQ-GFP-URA3 htb2::HTB2-mCherry-KANMX
SLJ2571 | Mata mps3Δ::NATMX ura3::MPS3-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ2694 | Mata mps3Δ::NATMX htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4310 | Mata mps3Δ::NATMX ura3::mps3∆TM(Mps2TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4313 | Mata mps3Δ::NATMX ura3::mps3∆TM(Ire1TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4320 | Mata mps3Δ::NATMX ura3::mps3∆TM(Heh1TM2)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
[NAME] | Mata mps3Δ::NATMX ura3::mps3∆TM(Pho8TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
[NAME] | Mata mps3Δ::NATMX ura3::mps3∆TM(Nem1TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX  pTRP1-MPS3
SLJ4420 | Mata mps3Δ::NATMX ura3::mps3∆TM(Kar1TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4555 | Mata mps3Δ::NATMX ura3::mps3∆TM(Heh1TM1)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4422 | Mata mps3Δ::NATMX ura3::mps3∆TM(Prm3TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4560 | Mata mps3Δ::NATMX ura3::mps3∆TM(Doa10TM1)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4388 | Mata mps3Δ::NATMX ura3::mps3∆TM(Erv41TM2)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4386 | Mata mps3Δ::NATMX ura3::mps3∆TM(Kex2TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4384 | Mata mps3Δ::NATMX ura3::mps3∆TM(Sso1TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ4592 | Mata mps3Δ::NATMX ura3::mps3∆(Pep12TM)-GFP-URA3 htb2::HTB2-mCherry-KANMX pTRP1-MPS3
SLJ001 | Mata
SLJ995 | [NAME]
[NAME] | [NAME]
SLJ4859 | [NAME] pSPC42-mCherry-HIS3
SLJ4864 | Mata ura3::gal-MPS3-G186K-URA3  spc110::SPC110-GFP-KANMX pSPC42-mCherry-HIS3
SLJ5097 | [NAME] nup49::NUP49-GFP-NATMX
SLJ5098 | [NAME] nup49::NUP49-GFP-NATMX
SLJ4963 | [NAME]  trp1::HDEL-dsRed-TRP1  pLEU2-GFP-PUS1
SLJ4965 | Mata ura3::gal-MPS3-G186K-URA3 trp1::HDEL-dsRed-TRP1  pLEU2-GFP-PUS1
SLJ5384 | [NAME]
SLJ5093 | [NAME]
SLJ5094 | [NAME]
[NAME] | [NAME]-3xFLAG-URA3-KANMX
[NAME] | [NAME]
SLJ715 | Mata spc42-11
SLJ1740 | Mata ndc1Δ::KANMX::ndc1-39-TRP1
SLJ910 | Mata/Mata mps3-1/mps3-1
SLJ1779 | Mata mps3∆::HIS3 leu2::MPS3-LEU2
SLJ1781 | Mata mps3∆::HIS3 leu2::mps3-Y502H-LEU2
SLJ1783 | Mata mps3∆::HIS3 leu2::mps3-Q572LQ573L-LEU2
SLJ1785 | Mata/Mata mps3∆::HIS3/mps3D::HIS3 leu2::mps3-W487A-LEU2/leu2::mps3-W487A-LEU2
SLJ1787 | Mata/Mata mps3∆::HIS3/mps3∆::HIS3 leu2::mps3-A540D-LEU2/leu2::mps3-A540D-LEU2
SLJ1789 | Mata/Mata mps3∆::HIS3/mps3√::HIS3 leu2::2x(mps3-∆SUN2)-LEU2/leu2::2x(mps3-∆SUN2)-LEU2
SLJ1791 | Mata mps3∆::HIS3 leu2::mps3-F592S-LEU2
SLJ1793 | Mata/Mata mps3∆::HIS3/mps3∆::HIS3 leu2::2x(mps3-W477A)-LEU2/leu2::2x(mps3-W477A)-LEU2
SLJ4259/SLJ5249 | Mata pom152Δ::HYGMX mps3Δ::NATMX
SLJ4260 | Mata pom152Δ::HYGMX
* pTRP1-MPS3 or pURA3-MPS3 were removed by plating cells to 5-FBA or 5-FOA, respectively, immediately before the experiment