Spreadsheet: 4611c433-1976-4f96-8c9b-6e6c63d22271
Sheet: Sheet1

Random or Selected mutant | Gene | Insertion range predicted by TMDH | Insertion position verified by sequencing | Sequence confirms TMDH prediction? | Notes
Random (Set6) | aroA | 1019537-1019724 | 1019584 | Yes
Random (Set6) | Intergenic: yeaG-mipA | 1320636-1320781 | 1320697 | Yes
Random (Set6) | ydiS | 1389118-1389214 | 1389174 | Yes
Random (Set6) | ydiB | 1398051-1398177 | 1398079 | Yes
Random (Set6) | [NAME] | 1564394-1564611 | 1564566 | Yes
Random (Set6) | pqaA | 1577284-1577472 | 1577389 | Yes
Random (Set6) | [NAME] | 1605832-1605990 | 1605949 | Yes
Random (Set6) | narZ | 1620369-1620625 | 1620473 | Yes
Random (Set6) | yncD | 1631777-1632003 | 1631886 | Yes
Random (Set6) | pykA | 1940149-1940227 | 1940160 | Yes
Random (Set6) | Overlaps: STM0019; Intergenic: STM0019-STM0020 | 23000-23192 | 23082 | Yes
Random (Set6) | sopD | 3109288-3109464 | 3109323 | Yes
Random (Set6) | [NAME] | 3192761-3192904 | 3192809 | Yes
Random (Set6) | [NAME] | 3321687-3321732 | 3321698 | Yes
Random (Set6) | hybA | 3332776-3332860 | 3332833 | Yes
Random (Set6) | Intergenic: aroK-hofQ | 3664347-3664578 | 3664478 | Yes
Random (Set6) | [NAME] | 3668529-3668637 | 3669381 | No | Insertion is within small  restriction fragment with only one probe. Insertion is in the centre of the region detected by the probe.
Random (Set6) | lpfE | 3844206-3844368 | 3844290 | Yes
Random (Set6) | rfaZ | 3932274-3932437 | 3932414 | Yes
Random (Set6) | rfaB | 3935912-3936030 | 3936078 | No | Microarray data suggests two transposon insertions close together and in same orientation
Random (Set6) | sugR | 3972248-3972471 | 3972275 | Yes
Random (Set6) | fidL | 3979409-3979555 | 3979471 | Yes
Random (Set6) | STM3781 | 4002070-4002256 | 4002218 | Yes
Random (Set6) | STM3820 | 4041098-4041313 | 4041258 | Yes
Random (Set6) | wzxE | 4157434-4157591 | 4157504 | Yes
Random (Set6) | yihW | 4257462-4257625 | 4257541 | Yes
Random (Set6) | Intergenic: STM4203-STM4204; Overlaps: STM4204 | 4445580-4445739 | 4445680 | Yes
Random (Set6) | yjeH | 4593748-4593939 | 4593912 | Yes
Random (Set6) | STM4489 | 4755370-4755532 | 4755471 | Yes
Random (Set6) | [NAME] | 4777960-4778115 | 4777260 | No | Two insertions in same orientation within large restriction fragment – signals interfere
Random (Set6) | [NAME] | 4779031-4779112 | 4779043 | Yes
Random (Set6) | Intergenic: yjiH-yjiJ | 4789483-4789732 | 4789679 | Yes
Random (Set6) | hsdS | 4798772-4799033 | 4798869 | Yes
Random (Set6) | Overlaps: sthA; sthA | 4872634-4872837 | 4872663 | Yes
Random (Set6) | glxR | 580374-580464 | 580462 | Yes
Random (Set6) | Intergenic: ybgL-STM0715; STM0715 | 780762-780912 | 780807 | Yes
Random (Set6) | STM0723 | No insertion predicted | 787805 | No | Low [NAME]. Insertion correctly predicted but omitted as signal was below cut-off.
Selected (Set1) | ldcC | 276725-276896 | 276796 | Yes
Selected (Set1) | [NAME] | 2427992-2428072 | 2428003 | Yes
Selected (Set1) | tolA | 815071-815242 | 815165 | Yes
Selected (Set1) | trkH | 4215924-4216056 | 4216019 | Yes
Selected (Set1) | trxA | 4146486-4146578 | 4146533 | Yes
Selected (Set1) | ybjG | 937157-937312 | 937212 | Yes
Selected (Set3) | atpA | 4098006-4098104 | 4098100 | Yes
Selected (Set3) | gidA | 4103450-4103570 | 4103497 | Yes
Selected (Set3) | [NAME] | 2432999-2433067 | 2433030 | Yes
Selected (Set3) | pduC | 2117011-2117174 | 2117087 | Yes
Selected (Set3) | sdhA | 798341-798441 | 798403 | Yes
Selected (Set3) | trkH | 4216090-4216216 | 4216133 | Yes
Selected (Set3) | Intergenic: trmA - btuB | 4369198-4369294 | 4369209 | Yes