Spreadsheet: 1fb52fc7-08a6-41e0-827c-2bf27361d57e
Sheet: Sheet1

[NAME] | 5' primer | Restiction site | 3' primer | Restriction site | Amount of gene amplified | Unnamed: 6 | Unnamed: 7 | Unnamed: 8
PFC0435w | [NAME] TTA ATGAAGGTTGGAATTATATTTTTTTGTTTATTTTTTTTTGTGGTTCTTGG | Gateway | A GAA AGC TGG GTT TGAA TTT TGA TCA TTT TGG AAA TTA ACA GGT GGT TGC GC | Gateway | all
PF10_0177 | [NAME] TTA ATGGATGTACAAAAAAAAAAATGCAAAACGTATTATTTGATAGC | Gateway | A GAA AGC TGG GTT GACAT GCA CTG ATT TTC TAT AGA AGT AAT ACG ATT GTA TGT CCA G | Gateway | 3027/3045 bp  (first annotated exon)
PF14_0607 | [NAME] TTA ATGCTGATGTTGTATCTTCATATTTTGTTATGTATTTTCTTAAATAATCC | Gateway | A GAA AGC TGG GTT ATC AAT GAG GTT TCT GAT TTT TCC ATG TGG CC | Gateway | 3099/9496 bp (cDNA)
PF13_0317 | GGAGgaattcATGAATGTATCAAGGGTATTATTAAATAACTCTAAAATATTAAAGAGG | EcoRI | GGACggattcATG TAG GTG GAC GTG TAA ATC TGA TGG GG | BamHI | all
PF13_0090 | GGACgaattcATG AAT GTT TTA GAT GTT TTA AAA AGA TGG TTT ATT CTT G | EcoRI | GGACggatccTAA TTC CAA ATG TTT AGT AGC TGT TCT TCT ATC AAT AC | BamHI | all
PF13_0218 | GGAGgaattcATGAATGTATCAAGGGTATTATTAAATAACTCTAAAATATTAAAGAGG | SalI | GGAC cccggg  TAC AGC AGG CTT ATG TAG GGA GAA TAT ATC C | SmaI | all
PFA0210c | GGAC gaattc ATG AGT TTA AAA AGA AGA AAA TTT ATT TTG TTG TCT TTT G | EcoRI | GGAC ACTAGT GTC CTT ATT AAA AAA TAT ACC AAA TAT TTT TTT AAA AAA GTT AAC GTT GAT GAT CC | SpeI | all
PFC0555c | GGAC ggatcc ATG TTT CGG TCG AAG GCT CAT TTT TTG ATG C | BamHI | GGAC ACTAGT ATC TAT TTG TGT TTG GTT ATA ATT TTC CTT TGA TGT TTC C | SpeI | all
PFL0600w | GGACgaattcATGATATATTATATAAGAAAAGTCGCTATTAACTTTTTG | EcoRI | GGACggatccAAA ATT TAA AGA TAA GGA TAG ACC TAG GTT ACC ATA TGG | BamHI | all
PFL1660c | [NAME] TTA ATGTTATTCCTTTTGCATATATATATACCACAAAATTCGGTTGC | Gateway | A GAA AGC TGG GTT GGA ATC GAC TTT TGC CTC TGC ATT AAT TAC ACT GTT C | Gateway | 1434/1885 bp  (cDNA)
PFD0495c | aaaaagcaggcttcgaaggagatagaaccatgATGTATAAGAAATGTTTCATTTTATATCCTATCTTTTTTC | Gateway | agaaagctgggtcTCATCTGTCGTCGGAACGGAAGGAATC | BamHI | all
Primers for P. berghei deletion constructs
Gene ID Pb | 5' primer upstream region | Restiction site | 3' primer upstream region | Restiction site | 5' primer downstream region |  | 3' primer downstream region
PB001106.03.0 | [NAME] T ccgcgg CCG AGA ATG TAT ATA TAC TAA ACG TGC TTA GCT | SacII | A GAA AGC TGG GT actagt CTT TCG CTT TTT TGC ATG AAA TAA ATC ACT GTG | SpeI | [NAME] T aagctt CCAATAACTAatTGTGttACTTAttctGGGCGTTC | HindIII | A GAA AGC TGG GT ggtacc TGG ACT ATA ATT ATA TTT TTT CAA GTT ATG TAT TAC ATC CAG | KpnI
PB001047.00.0 | [NAME] T ccgcgg GTC TTG TAT TTT TCC TTT TAA CCA AGT GG | SacII | A GAA AGC TGG GT actagt CAC ATA TAT TAT ATA CTA GGA TTG TTG GCC TTA [NAME] | SpeI | [NAME] T aagctt CTG TTC GTA CTG CAA AAA TAT CAT CTC GC | HindIII | A GAA AGC TGG GT ggtacc ATC ACA CCC ATT TCC TAC ATG TAT TCA TAT TAA TTC G | KpnI
PB000015.03.0 | [NAME] T ccgcgg CTG TGC ACC TGA TTG CCT ACA CCG | SacII | A GAA AGC TGG GT actagt CGTCCTAAAAATTACATGCTGTGTTGTGTATTTC | SpeI | [NAME] T aagctt CGA AGA AGT CAC ATT GCA GCC C | HindIII | A GAA AGC TGG GT ggtacc ATC ACA CCC ATT TCC TAC ATG TAT TCA TAT TAA TTC G | KpnI
PB000056.02.0 | [NAME] T  ccgcgg GGG TTT TCA CAT TTT TTT GCA GAG GTT CAT G | SacII | A GAA AGC TGG GT actagt GCA TTG TGA GGT TAT ATC ATA ATA TTG TGT TCA TG | SpeI | [NAME] T aagctt CAT TCA TTC GTT ACA TTA TCT CAT TTC ACC CC | HindIII | A GAA AGC TGG GT ggtacc CGG AAG TTT GCA TAG ATA ATT TGA AAG AGG G | KpnI
PB000767.00.0 | [NAME] T ccgcgg CGTAATGCTCTTATTAATAGGAATAATTTAAAATATTTTGGAAGG | SacII | A GAA AGC TGG GT actagt TA AAT TGT AAG AAC AAT TAT GCC CTA TTT CAT AAA AAT TTA TTT C | SpeI | [NAME] T aagctt CCT TTA TTT CTT GTT TGA TTT TAT TTT CCC CAT TTT AAC G | HindIII | A GAA AGC TGG GT ggtacc CCT TTA GAT TAC GAA TTT AAG GGC ATT CAA AGT ACC | KpnI
PB000651.03.0 | [NAME] T ccgcgg GCT TCC TTT TTT GCT TCC TTA TCT CCC | SacII | A GAA AGC TGG GT actagt CCG CAG GTT TGT TAC CAT TAA AAA ATG GG | SpeI | [NAME] T aagctt CCC TAT CAT TAT CGC TAC CTT TCA GCG | HindIII | A GAA AGC TGG GT ggtacc GGC AAT CCT AAG GAA TTG GCA AAT GC | KpnI
PB00259.00.0 | [NAME] T ccgcgg GGC TAG CTA ATA AAA TAC CTG AAT GAA AAG CCA TC | SacII | A GAA AGC TGG GT actagt CCG CAG GTT TGT TAC CAT TAA AAA ATG GG | SpeI | [NAME] T aagctt CGCTCATGCACACATATGGCTTATTAAAAACAC | HindIII | A GAA AGC TGG GT ggtacc CAT GGA CAC ATG AAA TAA GAC AAG GTA TTA TCA AC | KpnI
PB001041.00.0 | [NAME] T ccgcgg CAA TTG TTG ATT GAA AAA AAA TAA TAT AAC AAA TAA ATA TCA CAC | SacII | A GAA AGC TGG GT actagt CCC AGT ATT GTT CTT TCC ATT GAT TCC ACC | SpeI | [NAME] T aagctt CGT TAA AGC TCT CCC TTT TGG TAA TCT TGG | HindIII | A GAA AGC TGG GT ggtacc CCT ATC TAC TAA GGT TGG CTC CAT ATA AAT TGG G | KpnI
PB000828.02.0 | [NAME] T ccgcgg GCA TTA TTT CTG TTA TTT TAT GAT TAA AAT GTG CC | SacII | A GAA AGC TGG GT actagt GCT AGC TGC ATG TAA ATG CGT GTG C | SpeI | [NAME] T aagctt CTT GAT TTT CAG GTA TTT CCC AAT CTA AAT GCC | HindIII | A GAA AGC TGG GT ggtacc GAA TGC TTT CTA TGA ACC TCA GTT TCT CTA ATT TC | KpnI
Mutation pf14_0607
Mutation | Upstream 5' primer |  | Upstream 3' primer |  | Downstream 5' primer |  | Downstream 3' primer
N-terminal region | [NAME] TTA ATGCTGATGTTGTATCTTCATATTTTGTTATGTATTTTCTTAAATAATCC |  | A GAA AGC TGG GTT ATC AAT GAG GTT TCT GAT TTT TCC ATG TGG CC
Motif replacement | [NAME] TTA ATGCTGATGTTGTATCTTCATATTTTGTTATGTATTTTCTTAAATAATCC |  | CAT CAC TAC CTT CGA AAT CTG ATG CAT TAT TCA ACT TTT CAT AAA GC |  | CGC TTTATGAAAA GTTGAATAAT gca tca gat TTCGAAGGTA GTGATG |  | A GAA AGC TGG GTT ATC AAT GAG GTT TCT GAT TTT TCC ATG TGG CC
F-A replacement | [NAME] TTA ATGCTGATGTTGTATCTTCATATTTTGTTATGTATTTTCTTAAATAATCC |  | GAG AAA TCA TCA CTA CCT TCT GCC AAA ATA CGA TTA TTC AAC |  | GTTGAATAAT CGTATTTTG GCA GAAGGTAGTG ATGATTTCTC |  | A GAA AGC TGG GTT ATC AAT GAG GTT TCT GAT TTT TCC ATG TGG CC
AttB adapter primers
[NAME] | G GGG ACA AGT TTG TAC AAA AAA GCA GGC T
AttB2 | GGG GAC CAC TTT GTA CAA GAA AGC TGG GT
Table S7 - Primers used for amplification of genes and gene fragments.