Spreadsheet: 0fab0d5f-1374-40b8-8748-235929e70734
Sheet: Sheet1

Table S2. Primers and associated information | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5
Target | Primer | Sequence (5'-3') | Annealing Temp. | Extension Time | Application
NAIP | NAIPf-R | ctgtggtatccatgagctgtac | 55 | 1min | 5' RACE (outer)
NAIP | NAIPf-2R | [NAME] | 55 | 1min | 5' RACE (inner)
mNaipa | 1aORF-3'R | ggaagcttcctgaggctattgc | 58 | 1min | 5' RACE (outer)
mNaipa | 1aORF-R | ttgccagctgaactgcatccac | 58 | 1min | 5' RACE (inner)
mNaipb | 1bORF-3'R | ggaagtttcctgagtctggtgg | 58 | 1min | 5' RACE (outer)
mNaipb | 1bORF-R | caagactgaaagtgcatccactc | 58 | 1min | 5' RACE (inner)
mNaipe | 1eORF-R2 | caaaagcactgaacgccaagtc | 58 | 1min | 5' RACE (outer)
mNaipe | 1eRACE-R | [NAME] | 58 | 1min | 5' RACE (inner)
mNaipf | 1fORF-R2 | aaaagcactgaaccccaagtttc | 58 | 1min | 5' RACE (outer)
mNaipf | 1fRACE-R | gctgaactagattcatcccgaaac | 58 | 1min | 5' RACE (inner)
rNaip1/2 | RAT-outer-R | ttcacccgtatgtcgtacttgc | 55 | 1min | 5' RACE (outer)
rNaip1/2 | RAT-inner-R | atctttgcccgagaggaactc | 55 | 1min | 5' RACE (inner)
NAIP | NAIPb-F | ctcttcgactgcctgttcatc | 55 | 1min | RT-PCR (LTR-Xu et al.)
NAIP | [NAME] | gtgaactcagacagaagtgtgg | 54-58 | 0.5-1min | RT-PCR (LTR-TestisTSS)
NAIP | NAIPf-2R | [NAME] | 54-58 | 0.5-1min | RT-PCR (LTR)
NAIP | NAIPf-F | gtacagctcatggataccacag | 55 | 1min | RT-PCR (ORF)
NAIP | NAIPd-R | cccttgtagctttgaatatactg | 55 | 1min | RT-PCR (ORF)
GAPDH | GAPDH-F | catgagaagtatgacaacagcctc | 55 | 0.5min | RT-PCR (control)
GAPDH | GAPDH-R | [NAME] | 55 | 0.5min | RT-PCR (control)
mNaipa | 5'1aORR1E-F | ggcggagcctcatcagagg | 60 | 30sec | RT-PCR (upstream 1aLTR)
mNaipa | 1aMTE-F | tccttctaccaaaccttccgtg | 58 | 45sec | RT-PCR (1aLTR)
mNaipa | 1aORF-R | ttgccagctgaactgcatccac | 58 | 45sec | RT-PCR (1aLTR)
mNaipa | 1aORFb-F | ggtggatgcagttcagctg | 58 | 45sec | RT-PCR (1aORF)
mNaipa | 1aORFb-R | acaaatccctcatagccttgg | 58 | 45sec | RT-PCR (1aORF)
mNaipb | 5'1bORR1E-F | gaggtggagcatggtggag | 59 | 30sec | RT-PCR (upstream 1bLTR)
mNaipb | 1bMTE-F | gctcctgccaagccttctgtg | 58 | 45sec | RT-PCR (1bLTR)
mNaipb | 1bORF-R | caagactgaaagtgcatccactc | 58 | 45sec | RT-PCR (1bLTR)
mNaipb | 1bORFb-F | agagtggatgcactttcagtc | 58 | 45sec | RT-PCR (1bORF)
mNaipb | 1bORFb-R | catattgggtaatttcctctgg | 58 | 45sec | RT-PCR (1bORF)
mNaipe/f | 5'1e/1fORR1E-F | aggcggggcatggtgaag | 58 | 30sec | RT-PCR (upstream 1e/1fLTR)
mNaipe | 1eMTE-F | ccttgagatacagatcttcagag | 58 | 45sec | RT-PCR (1eLTR)
mNaipe | 1eORF-R | gaaggaactcacattccggac | 58 | 45sec | RT-PCR (1eLTR)
mNaipe | 1eORF-F | tgctcttctcggggtggatgc | 58 | 45sec | RT-PCR (1eORF)
mNaipe | 1eORF-3'R | [NAME] | 58 | 45sec | RT-PCR (1eORF)
mNaipf | 1fMTE-F | tgtgaccagctggcttcctgc | 58 | 45sec | RT-PCR (1fLTR)
mNaipf | 1fORF-R | tgaaggaactcacattctggtc | 58 | 45sec | RT-PCR (1fLTR)
mNaipf | 1fORF-F | gctcgtttcgggatgaatctag | 58 | 45sec | RT-PCR (1fORF)
mNaipf | 1fORF-3'R | catgaacaaatccctcgtagtc | 58 | 45sec | RT-PCR (1fORF)
gapdh | gapdh-f | gtggcagtgatggcatggac | 55 | 0.5min | RT_PCR (control)
gapdh | gapdh-r | gtggagtctactggtgtcttc | 55 | 0.5min | RT_PCR (control)
rNaip1 | Naip1MTE-F | tgctccttctaccacagcttc | 59 | 30sec | RT-PCR (LTR)
rNaip1 | Naip1ORF-R | ggtcgtaatttcctgtggctc | 59 | 30sec | RT-PCR (LTR)
rNaip1 | 1aORFb-F | ggtggatgcagttcagctg | 59 | 45sec | RT-PCR (ORF)
rNaip1 | Naip1ORF-3'R | catgaagaaatcccgtgtagc | 59 | 45sec | RT-PCR (ORF)
rNaip2 | Naip2MTE-F | tgtgaccagctggtttcctg | 59 | 30sec | RT-PCR (LTR)
rNaip2 | Naip2ORF-R | ctggttgtaatttccggtgaac | 59 | 30sec | RT-PCR (LTR)
rNaip2 | Naip2ORF-F | agttcaccggaaattacaaccag | 59 | 45sec | RT-PCR (ORF)
rNaip2 | Naip2ORF-F.2 | tgccaagtacatacaaacctacg | 59 | 30sec | RT-PCR (ORF)
rNaip2 | Naip2ORF-3'R | catgaagaaatccctcgtagg | 59 | 45sec | RT-PCR (ORF)
rNaip2 | Naip2ORF-3'R.2 | cagaagaatgaactgccactcc | 59 | 30sec | RT-PCR (ORF)
GAPDH | [NAME] | cccatcaccatcttccaggag | see Methods | see Methods | qRT-PCR (control)
GAPDH | qGAPDH-R | cttctccatggtggtgaagacg | see Methods | see Methods | qRT-PCR (control)
NAIP | qTotal-F | gcattctcctctattagactag | see Methods | see Methods | qRT-PCR (Total)
NAIP | qTotal-R | gccaactgaactgcatctag | see Methods | see Methods | qRT-PCR (Total)
NAIP | qLTR-F2 | ggaaaacccatatatttgatgtc | see Methods | see Methods | qRT-PCR (LTR)
NAIP | [NAME] | [NAME] | see Methods | see Methods | qRT-PCR (LTR)
mNaipa | 1aCPE-F3 | agctcttccggaaagttcctggagaatactg | 55 | 0.5min | genomic PCR
mNaipb | 1bCPE-F3 | agctcttaagactgtgtctgggcctacactg | 55 | 0.5min | genomic PCR
mNaipe/f | 1e/fCPE-F3 | agctcttaaggagtttggatcctttaagaggc | 55 | 0.5min | genomic PCR
mNaipa | 1aCPE-R3 | agctaagcttagttacttcatgttcttcaacac | 55 | 0.5min | genomic PCR
mNaipb/e/f | 1b/e/fCPE-R3 | agctaagctttactttcatttcccctcgcac | 55 | 0.5min | genomic PCR
Primer name suffixes F and R, distinguish forward and reverse primers, respectively.