Spreadsheet: bcbe45f0-9a2d-4375-a078-9f21b6117145
Sheet: Sheet1

Test Referenced | Entry Type | Mayo ID | Description | Reporting Description | Method | Collection Container | Temperature | Stability | [NAME] | Unit of Measure | [NAME] | Minimum Volume | Reject Due To | Reference Values | Days/Times Performed | Analytic Time | Maximum Lab Time | CPT Units | CPT Code | LOINC® Code | LOINC® Attributes | Always Performed | Orderable Separately | Performing Location
PEWE | Test | PEWE | Porphyrins Evaluation, Washed Erythrocytes | [NAME], RBC | PEWE/31893: Spectrofluorometric PPFR/28116, PFR/28117: High-Performance Liquid Chromatography (HPLC) |  |  |  |  |  | All porphyrin tests on erythrocytes can be performed on 1 draw tube.    Collection Container/Tube:  Preferred: Green top (sodium heparin) Acceptable: Metal free, lavender top (EDTA) or green top (lithium heparin) Submission Container/Tube: Plastic vial Specimen Volume: Washed erythrocyte suspension Collection Instructions: 1. Patient should abstain from alcohol for 24 hours. 2. Collect and process whole blood specimen as follows: a. Immediately place specimen on wet ice. b. Transfer entire specimen to a 12-mL graduated centrifuge tube. [NAME] specimen for 10 minutes at 2,000 rpm. d. Record volume of packed cells and the total volume of the specimen. [NAME] supernatant plasma. f. [NAME] packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing. g. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.  Additional Information: 1. Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen. 2. Include a list of medications the patient is currently taking. Forms: 1.	1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (Supply T576) is available in Special Instructions. 2.	2. If not ordering electronically, submit a Biochemical Genetics Request Form (Supply T439) with the specimen. | 1 mL of washed and resuspended e | Hemolysis	NA Thawing	Warm reject; Cold OK Lipemia	NA Icterus	NA Other	Cell suspension not available | PORPHYRINS, TOTAL, RBC <80 mcg/[NAME] packed cells  FREE PROTOPORPHYRIN <20 mcg/[NAME] packed cells  ZINC-COMPLEXED PROTOPORPHYRIN <60 mcg/[NAME] packed cells  UROPORPHYRIN < or =2 mcg/[NAME] packed cells  HEPTACARBOXYLPORPHYRIN < or =1 mcg/[NAME] packed cells  HEXACARBOXYLPORPHYRIN < or =1 mcg/[NAME] packed cells  PENTACARBOXYLPORPHYRIN < or =1 mcg/[NAME] packed cells  COPROPORPHYRIN < or =2 mcg/[NAME] packed cells  PROTOPORPHYRIN <80 mcg/[NAME] packed cells | Monday through Friday; 8 a.m. | 3 days (not reported on Saturday or Sunday) | 4 days | 1 | 84311 |  |  |  |  | Rochester
PEWE | [NAME] | WRBC | Washed RBC |  |  |  | Frozen | 14 days
PEWE | Order Question | BG569 | Total cell suspension |  | Plain Text
PEWE | Order Question | BG570 | Packed cell volume |  | Plain Text
PEWE | Result | 31942 | Total Porphyrins, RBC | Total Porphyrins, RBC | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 2814-2
PEWE | Result | 31943 | Interpretation | Interpretation | Alphanumeric |  |  |  |  |  |  |  |  |  |  |  |  |  |  | 59462-2
PEWE | Result | BG569 | Total cell suspension | Total cell suspension | Alphanumeric |  |  |  |  |  |  |  |  |  |  |  |  |  |  | In Process
PEWE | Result | BG570 | Packed cell volume | Packed cell volume | Alphanumeric |  |  |  |  |  |  |  |  |  |  |  |  |  |  | In Process
PEWE | [NAME] | PFR | Porphyrins, Fractionation, [NAME] | Porphyrins, Fractionation | Only orderable as part of a reflex test. For further information see PEE/88886 [NAME], Erythrocytes. |  |  |  |  |  | Only orderable as part of a reflex test. For further information see PEE/88886 [NAME], Erythrocytes. | Whole blood: 3 mL Washed & resus |  | Only orderable as part of a reflex test. For further information see PEE/88886 [NAME], Erythrocytes. | Monday through Friday; 8 a.m. | 2 days (not reported on Saturday or Sunday) | 3 days | 1 | 82492 |  |  | No | No
PFR | [NAME] | WRBC | Washed RBC |  |  |  | Frozen | 21 days
PFR | Result | 2340 | Uroporphyrin | Uroporphyrin | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 14180-4
PFR | Result | 2341 | Heptacarboxyl Porphyrins | Heptacarboxyl Porphyrins | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 9446-6
PFR | Result | 2346 | Hexacarboxyl Porphyrins | Hexacarboxyl Porphyrins | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 16963-1
PFR | Result | 2347 | Pentacarboxyl Porphyrins | Pentacarboxyl Porphyrins | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 18250-1
PFR | Result | 2329 | Coproporphyrin | Coproporphyrin | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 18257-6
PFR | Result | 2328 | Protoporphyrin | Protoporphyrin | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 2896-9
PFR | Result | 29355 | Interpretation | Interpretation | Alphanumeric |  |  |  |  |  |  |  |  |  |  |  |  |  |  | 59462-2
PEWE | [NAME] | PPFR | Protoporphyrins, Fractionation, [NAME] | Protoporphyrins, Fractionation | Only orderable as part of a reflex test. For further information see PEE/88886 [NAME], Erythrocytes. |  |  |  |  |  | Only orderable as part of a reflex test. For further information see PEE/88886 [NAME], Erythrocytes. | Whole blood: 3 mL Washed & resus | Specimens Other Than:		Frozen erythrocyte suspension or  refrigerated, whole blood Anticoagulants Other Than: Hemolysis:			Yes Thawing:			Warm reject; Cold Ok; Note  to Lab Lipemia:			No | Only orderable as part of a reflex test. For further information see PEE/88886 [NAME], Erythrocytes. | Monday through Friday; 8 a.m. | 2 days (not reported on Saturday or Sunday) | 3 days | 1 | 84202 |  |  | No | Yes (order PPFE)
PPFR | [NAME] | VARIES | Varies |  |  |  | Frozen
PPFR | Result | 2327 | Zinc-Complexed Protoporphyrin | Zinc-Complexed Protoporphyrin | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 33007-6
PPFR | Result | 2326 | Free Protoporphyrin | Free Protoporphyrin | Numeric |  |  |  |  | mcg/dL |  |  |  |  |  |  |  |  |  | 2892-8
PPFR | Result | 28434 | Interpretation | Interpretation | Alphanumeric |  |  |  |  |  |  |  |  |  |  |  |  |  |  | 59462-2