Spreadsheet: 96a4f872-880a-4b8a-aad2-ffcdc87bdeae
Sheet: Sheet1

Table S5: Phosphopeptide identification and quantification of anti-GAP45 column eluates (MaxQuant Report) | Unnamed: 1 | Unnamed: 2 | Unnamed: 3 | Unnamed: 4 | Unnamed: 5 | Unnamed: 6 | Unnamed: 7 | Unnamed: 8 | Unnamed: 9 | Unnamed: 10
Protein | [NAME] | aa | Pos | LocProb | [NAME] | Phospho (STY) Probabilities | [NAME] | Ratio H/L | Ratio H/L Normalized by Protein | Manual validation
42.m08072 | hypothetical protein (GAP45) | S | 153 | 1 | _QMQEALKQEEMS(ph)PREK_ | QMQEALKQEEMS(1)PREK | 52.55 | 1.6583 | 0.99715 | correct
42.m08072 | hypothetical protein (GAP45) | Y | 158 | 0.993498 | _Y(ph)DKLASPEDSASETTMATQPQK_ | Y(0.993)DKLAS(0.005)PEDS(0.001)ASETTMATQPQK | 70.2 | 1.7061 | 0.99499 | correct
42.m08072 | hypothetical protein (GAP45) | S | 163 | 0.999987 | _LAS(ph)PEDSASETTM(ox)ATQPQK_ | LAS(1)PEDSASETTMATQPQK | 57.46 | 1.7065 | 0.99522 | correct
42.m08072 | hypothetical protein (GAP45) | S | 167 | 0.498011 | _YDKLAS(ph)PEDSASETTMATQPQK_ | YDKLAS(0.498)PEDS(0.498)AS(0.004)ETTMATQPQK | 57.46 |  |  | incorrect
42.m08072 | hypothetical protein (GAP45) | S | 169 | 0.933387 | _LASPEDSAS(ph)ETTMATQPQK_ | LASPEDS(0.066)AS(0.933)ETTMATQPQK | 57.46 | 1.8146 | 1.0583 | correct
42.m08072 | hypothetical protein (GAP45) | S | 184 | 0.499987 | _VAEHS(ph)SAAVTDR_ | VAEHS(0.5)S(0.5)AAVTDR | 61.92 | 6.7211 | 3.9197 | correct*
42.m08072 | hypothetical protein (GAP45) | S | 185 | 0.499987 | _VAEHSS(ph)AAVTDR_ | VAEHS(0.5)S(0.5)AAVTDR | 61.92 | 6.7211 | 3.9197 | correct*
42.m08072 | hypothetical protein (GAP45) | T | 189 | 0.999999 | _VAEHSSAAVT(ph)DR_ | VAEHSSAAVT(1)DR | 61.92 | 6.2837 | 3.6647 | correct
42.m08072 | hypothetical protein (GAP45) | S | 239 | 1 | _HIDLS(ph)DAPLLN_ | HIDLS(1)DAPLLN | 13.85 |  |  | incorrect
B6KB18 | Myosin light chain (TgMLC1) | S | 55 | 0.99974 | _VGEYDGACES(ph)PSCR_ | VGEYDGACES(1)PSCR | 41.03 | 2.8271 | 0.99574 | correct
B6KB18 | Myosin light chain (TgMLC1) | T | 98 | 0.988892 | _VST(ph)GDAMILAR_ | VS(0.011)T(0.989)GDAMILAR | 34.63 | 4.3317 | 1.6491 | correct
B6KB18 | Myosin light chain (TgMLC1) | S | 132 | 1 | _SGDNLDYAS(ph)FQK_ | SGDNLDYAS(1)FQK | 45.23 | 4.7772 | 1.6981 | correct
46.m00001 | Myosin light chain (TgMyoA) | S | 20 | 0.77047 | _S(ph)SDVHAVDHSGNVYK_ | S(0.77)S(0.229)DVHAVDHSGNVYK | 29.29 | 3.1296 | 1.284 | correct
B9PK54 | Calcium dependent ATPase, putative | S | 450 | 0.99914 | _ASS(ph)FDGAAAIR_ | AS(0.001)S(0.999)FDGAAAIR | 47.41 | 1.78 | 0.86536
B9PK54 | Calcium dependent ATPase, putative | S | 460 | 0.49974 | _ES(ph)SHGFETVVVR_ | ES(0.5)S(0.5)HGFET(0.001)VVVR | 33.94
B9PK54 | Calcium dependent ATPase, putative | S | 461 | 0.49974 | _ES(ph)SHGFETVVVR_ | ES(0.5)S(0.5)HGFET(0.001)VVVR | 33.94
B9PK54 | Calcium dependent ATPase, putative | T | 471 | 0.958688 | _T(ph)LGSLQTGDAATGVTLGR_ | T(0.959)LGS(0.041)LQTGDAATGVTLGR | 40.61
B9PK54 | Calcium dependent ATPase, putative | S | 719 | 0.956987 | _ETAFSSALDS(ph)AENLPAPGSSVDHR_ | ETAFS(0.008)S(0.035)ALDS(0.957)AENLPAPGSSVDHR | 13.86 | 2.377 | 1.1556
B9PK54 | Calcium dependent ATPase, putative | S | 1146 | 0.83176 | _NSGDSTGATS(ph)PASPGLLR_ | NSGDST(0.001)GAT(0.083)S(0.832)PAS(0.083)PGLLR | 47.81 | 1.5454 | 0.7513
[NAME] | Dense granule antigen protein GRA6 (Fragment) | S | 134 | 0.892039 | _RYS(ph)SVQEPQAK_ | RY(0.008)S(0.892)S(0.1)VQEPQAK | 14.84 | 0.21479 | 1.0255
[NAME] | Dense granule antigen protein GRA6 (Fragment) | S | 135 | 0.999135 | _[NAME]_ | [NAME] | 14.84 | 0.20425 | 0.97516
540.m00334 | zinc finger (C3HC4 RING finger) protein, putative | S | 506 | 0.816956 | _S(ph)GSLDADTQR_ | S(0.817)GS(0.183)LDADTQR | 32.12 | 1.0578
46.m01634 | transporter, putative | S | 96 | 0.999998 | _GPPAYS(ph)LEIR_ | GPPAYS(1)LEIR | 7.27
B6KHU7 | Translation initiation factor eIF-5A, putative | S | 2 | 0.999899 | _(ac)S(ph)DAEDVTFETADAGASHTYPMQAGAIK_ | S(1)DAEDVTFETADAGASHTYPMQAGAIK | 35.15 | 0.06105 | 0.43283
83.m02148 | translation initiation factor 3 subunit 8, putative | S | 287 | 1 | _TKDEGALS(ph)PR_ | TKDEGALS(1)PR | 24.92 | 0.16776
B6KFG2 | Syntaxin binding protein, putative | S | 544 | 0.988949 | _LAS(ph)ATEQTIGR_ | LAS(0.989)AT(0.011)EQTIGR | 28.53
Q9GSE9 | surface antigen Tax_Id=5811 | S | 201 | 0.936094 | _MGPSDIS(ph)THVR_ | MGPSDIS(0.936)T(0.063)HVR | 53.34 | 0.72874 | 1.1475
B9PUJ2 | Ribonuclease, putative | T | 738 | 0.299754 | _TSVHAET(ph)AEGASM(ox)PYRTRELR_ | T(0.128)S(0.139)VHAET(0.3)AEGAS(0.234)MPY(0.089)RT(0.11)RELR | 2.45
49.m05723 | ras-GTPase-activating protein binding protein, putative | S | 343 | 0.978028 | _[NAME]_ | EAVVPS(0.011)S(0.011)PS(0.978)PR | 20.84 | 0.6679 | 2.5291
B9Q026 | Putative uncharacterized protein | T | 232 | 1 | _[NAME]_ | [NAME] | 2.59 | 1.6763
83.m01196 | phosphatase domain-containing protein | S | 149 | 1 | _YAS(ph)QMLQDAESAR_ | YAS(1)QMLQDAESAR | 57.34 | 16.192
44.m00020 | pfs77-related | S | 548 | 1 | _AAS(ph)GVGLGEEAQISA_ | AAS(1)GVGLGEEAQISA | 25.75
55.m04819 | Na+/H+ antiporter-related | S | 2204 | 1 | _DFQEQS(ph)PR_ | [NAME] | 7.26
72.m00687 | lysine decarboxylase, putative | S | 112 | 0.925963 | _LSPSS(ph)FDAR_ | LSPS(0.074)S(0.926)FDAR | 16.06
35.m00901 | KH domain-containing protein | S | 259 | 0.985788 | _ALHASGS(ph)PGGER_ | ALHAS(0.014)GS(0.986)PGGER | 21.62 | 0.47392
A0SIY4 | Granule antigen protein GRA7 | S | 227 | 0.999687 | _SRQPALEQEVPES(ph)GEDGEDARQ_ | SRQPALEQEVPES(1)GEDGEDARQ | 7.8 | 0.60782 | 2.1723
55.m00233 | casein kinase II alpha subunit, putative | S | 238 | 0.929373 | _AAS(ph)ASSAGTTAGEAAR_ | [NAME] | 60.04 | 6.7395
B9PZH4 | [NAME] water dikinase, putative | S | 1353 | 1 | _GAS(ph)FVEEAR_ | GAS(1)FVEEAR | 15.15
42.m00015 | 28 kDa antigen | S | 184 | 1 | _AAHVPVPDFS(ph)Q_ | [NAME] | 23.59
49.m05723 | ras-GTPase-activating protein binding protein, putative | S | 400 | 0.997913 | _[NAME]_ | APNAPAT(0.002)PIS(0.998)PR | 23.51 | 0.10442 | 0.3954
72.m00380 | 3',5'-cyclic phosphodiesterase, putative | S | 1074 | 1 | _RAS(ph)LEPAGLEVGGA_ | RAS(1)LEPAGLEVGGA | 26.97 | 4.384
B9PMW9 | Dense granule protein, putative | S | 120 | 1 | _VEEELS(ph)LLRR_ | VEEELS(1)LLRR | 49.36 | 0.60383 | 1
Q0GC66 | Dense granule protein (Fragment) | S | 4 | 0.99974 | _(ac)ATAS(ph)DDELM(ox)SR_ | ATAS(1)DDELMSR | 24.36 | 0.59395 | 1
B9Q1A6 | Putative uncharacterized protein | S | 271 | 0.999961 | _[NAME] | ALGTRYDS(1)PR | 15.64 | 4.2737
B9PGN2 | Putative uncharacterized protein | T | 114 | 1 | _M(ox)QT(ph)DILEEHEQLM(ox)R_ | MQT(1)DILEEHEQLMR | 39.02 | 2.9135 | 1
B9PPL4 | Putative uncharacterized protein | S | 17 | 1 | _LHS(ph)CAGGEESSGQCLEK_ | [NAME] | 24.39 | 6.0644
B6K8V6 | Putative uncharacterized protein | S | 892 | 0.741353 | _TRGNS(ph)EVAGDGIVQVR_ | T(0.259)RGNS(0.741)EVAGDGIVQVR | 12.47
B6KMN7 | Putative uncharacterized protein | S | 132 | 1 | _REPS(ph)PPAR_ | REPS(1)PPAR | 19.52 | 2.2142
B9PHI3 | Putative uncharacterized protein | S | 5 | 1 | _S(ph)AEVVYATGLK_ | S(1)AEVVYATGLK | 48.67 | 0.025273
Q1JSJ0 | Putative uncharacterized protein | S | 556 | 1 | _ANS(ph)LQNLQR_ | ANS(1)LQNLQR | 27.73 | 2.6478
B9PKU6 | Putative uncharacterized protein | T | 40 | 1 | _YAGT(ph)EVIR_ | YAGT(1)EVIR | 44.01
50.m03340 | conserved hypothetical protein | S | 2 | 0.99118 | _(ac)S(ph)TLQDIR_ | S(0.991)T(0.009)LQDIR | 36.25 | 2.6282
583.m05501 | conserved hypothetical protein | S | 651 | 0.99955 | _GGS(ph)ESLAGESR_ | GGS(1)ESLAGESR | 36.41 | 8.2349
25.m02949 | hypothetical protein | S | 78 | 0.999611 | _NTLETGDS(ph)LR_ | NTLETGDS(1)LR | 29.51 | 0.81728 | 1.4977
25.m02949 | hypothetical protein | S | 183 | 0.999971 | _VMEDDS(ph)GDEDWEPSFR_ | VMEDDS(1)GDEDWEPSFR | 19.69 | 0.45678 | 0.83706
42.m00041 | hypothetical protein | S | 739 | 0.999412 | _LRS(ph)VSEDPEEVR_ | LRS(0.999)VS(0.001)EDPEEVR | 24.28
44.m00047 | hypothetical protein | S | 460 | 0.999997 | _EAES(ph)EDAEDTEE_ | EAES(1)EDAEDTEE | 30.04
44.m04614 | hypothetical protein | S | 58 | 1 | _GAEGQGLS(ph)PHQQR_ | GAEGQGLS(1)PHQQR | 8.27 | 1.0668
50.m03142 | hypothetical protein | S | 304 | 1 | _GRFS(ph)DDDMAR_ | GRFS(1)DDDMAR | 41.6 | 7.9895
583.m00634 | hypothetical protein | S | 312 | 0.659319 | _[NAME]_ | [NAME] | 36.01 | 5.5183
76.m01553 | hypothetical protein | S | 1043 | 0.821157 | _DARKVTDGLES(ph)K_ | [NAME] | 3.53
50.m03374 | hypothetical protein | T | 168 | 1 | _[NAME]_ | [NAME] | 15.49