Spreadsheet: 08615aaa-cdf4-4a75-bb24-0779efb7933a
Sheet: Sheet1

Instructions for AAV Reference Standard Characterization Report | Unnamed: 1
These instructions are intended to aid in the completion of this largely automated  report of assay data  associated with the characterization of AAV reference material.
General Guidelines:
1 | Save this file as data is entered with a name that includes your institution and your name.
2 | Due to the automatic nature of this report most cells are locked and can not be selected.
3 | Cells that require data are high-lighted green. Instructions for each sheet follow below.
4 | Spaces for institution, technician, reviewer, and all dates are unlocked but are not high-lighted.
5 | Once this report is completed save it with a name including your institution, your name, and the date submitted.
Summary Report
1 | Data values on this page  automatically fill as assay data is entered in its respective space.
2 | Values displayed on this sheet are simply copies of the data entered on other sheets.
3 | Upon review of reported data values the reviewer must enter their name and the date in the space provided below the data.
4 | When all assay data is reported, enter the institution name, submitted by, and date sections.
Physical Titer by qPCR
1 | Enter assay data in appropriate locations indicated by green high-light. Enter names and dates where indicated.
2 | PCR efficiency, [NAME], and Average GC/ mL are automatically calculated and reported to the summary report.
Identity and Purity by SDS PAGE
1 | Each column of each table poses a Yes/ No question. Enter Yes, No, or NA as appropriate in the green high-lighted cells. Enter names and dates where indicated.
2 | Gel B SYPRO Ruby Reduced also asks for % Purity. Enter this value as a number, i.e. enter 90% as 90.
3 | Following the guidelines outlined in the protocol, enter pass of fail in the indicated cell for each gel. This value is automatically entered in the summary report.
[NAME] by ELISA
1 | Enter A450, dilution, and particles/ mL, determined according to the protocol,  in green high-lighted cells.
2 | Enter names and dates where indicated.
3 | Average Particles/ mL is automatically calculated and entered in the summary report.
Infectious Titer by GFP
1 | Follow the directions to enter data in green high-lighted cells. Enter names and dates where indicated.
2 | Dilutions averaging less than 20 GFU/ well are labeled "low" and dilutions averaging greater than 300 GFU/ well are labeled "High".
3 | Dilutions with 20 to 300 GFU/ well are used to automatically calculate GFU/ mL. Averaged GFU/mL is automatically entered in the summary report.
4 | "Input Vector Physical Titer by qPCR" automatically appears as an average GC/ mL when the Physical Titer by qPCR assay data is entered. Pt:GFU ratios are automatically calculated and entered in the summary report.
Infectious Titer by TCID50
1 | Enter names and dates where indicated.
2 | Paste the pre-determined input-subtracted copies per well for each replicate of each dilution into the green high-lighted cells in the same orientation they were in on the PCR plate.
3 | Positive replicates  automatically high-light yellow and the number of positives in each dilution is automatically entered to the right of the table
4 | "Input Vector Physical Titer by qPCR" automatically appears as an average GC/ mL when the Physical Titer by qPCR assay data is entered. Input vector values are automatically calculated.
5 | Log(TCID50), standard error, coefficient of variation, [NAME], and P:I ratio are automatically entered as each trial worksheet is completed.
TCID50 Trial Worksheets
1 | Follow the directions to enter data in the green high-lighted cells. Enter names and dates where indicated.
2 | "Vector concentration (GC/ mL)" automatically appears as an average GC/ mL when the Physical Titer by qPCR assay data is entered.
3 | All calculations automatically complete, are entered in the Infectious Titer by TCID50 page and, subsequently, in the summary report.